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Jurnal Kedokteran Hewan

JKH
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Published by Universitas Syiah Kuala

ISSN : 1978225X EISSN : 25025600 DOI : 10.21157

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.

Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)

Articles 901 Documents

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IDENTIFIKASI PROTEIN PENENTU IMPLANTASI EMBRIO PADA TIKUS WISTAR (Rattus norvegicus) Agung Janika Sitasiwi; Muhammad Anwar Djaelani
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Penelitian dengan tujuan mengidentifikasi protein penentu implantasi embrio pada tikus Wistar (Rattus norvegicus) dilakukan sebagai upaya eksplorasi agen imunokontrasepsi baru untuk regulasi fertilitas hewan liar. Hewan uji yang digunakan adalah tikus Wistar betina dewasa kelamin dengan bobot badan berkisar 280-300 g. Tikus dibagi dalam dua kelompok, satu kelompok sebagai kontrol (tidak dikawinkan) sedangkan kelompok lain adalah kelompok yang dikawinkan. Tikus dipelihara dan dikawinkan dalam kondisi laboratorium yang terkontrol. Kebuntingan ditentukan dengan adanya vaginal plug pada tikus betina setelah dikawinkan. Protein diisolasi dari sampel uterus yang diambil pada umur kebuntingan 3-5 hari. Isolasi dan separasi protein dilakukan berdasarkan manual dan standar protein produk Bio-Rad. Hasil penelitian menunjukkan pita protein yang terekspresi pada hari ke-4 kebuntingan dengan berat molekul berkisar 45 kDa diduga leukemia inhibitory factor.

STUDI ANATOMIS DAN HISTOLOGIS PANKREAS BIAWAK AIR (Varanus salvator) (Anatomical and Histological Study of the Pancreas of Water Monitor Lizard (Varanus salvator)) Hamny Hamny; Muhammad Iqbal; Sri Wahyuni; Mustafa Sabri; M. Jalaluddin; Rinidar Rinidar
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

The aim of this research was to determine the anatomy and histology of pancreas of water monitor lizard (Varanus salvator). Two pancreas were used in this research (a male and a female). Water monitor lizard was euthanized using chloroform and subsequently necropsied to collect the pancreas. The pancreas was washed in 0.9 % NaCl solution. Anatomical observations were carried out including location, shape, and color of pancreas, also the length and weight measurements. Then, pancreas was fixed with 4% paraformaldehyde for 7 days to proceed to histological preparation and hematoxylin eosin (HE) staining. The results of the anatomical observations showed that pancreas of water monitor lizard have two lobes, dorsal lobe (splenic lobe) and ventral lobe (duodenal lobe). Dorsal lobe which was in oval form adhered with the spleen, and ventral lobe was located in the cranial of intestines with the form of long spherical. Both of pancreas lobes were white-yellow color with the length of 1.45±0.64 cm (dorsal lobe) and 7.00±0.42 cm (ventral lobe). Weight of dorsal lobe was 0.15±0.01 g and ventral lobe was 6.35±2.30 g. The histological observations found the acinar cells, ducts, and blood vessels in both of pancreas lobes. Additionally, the similar structure of Langerhans islet only found at the ventral lobe of pancreas. In conclusion, pancreas of water monitor lizard consists of two lobes which located differently. Ventral lobe is larger than the dorsal lobe. Both lobes have similar histological structure except the Langerhans islets are only appeared in the ventral lobe.

EVALUASI STATUS VIRULENSI ISOLAT Bacillus anthracis ASALNUSA TENGGARA DAN PAPUA MENGGUNAKAN METODE POLYMERASE CHAIN REACTION MULTIPLEX Maxs Urias Ebenhaizar Sanam; Widya Asmara; Agnesia Endang Tri Hastuti Wahyuni; Michael Haryadi Wibowo; Rahmat Setya Adji
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengevaluasi status virulensi 22 isolat Bacillus anthracis (B. anthracis) asal Nusa Tenggara dan Papua menggunakan metode polymerase chain reaction (PCR) multiplex dengan dua pasang primer nukleotida yang memiliki target amplifikasi gen spesifik pada kedua plasmid. Ektraksi DNA dilakukan dengan metode lisis panas. Pasangan primer PA5 dan PA8 digunakan untuk mengamplifikasi gen pagA pada pXO1, sedangkan pasangan primer 1234 F dan 1301 R mengamplifikasi gen capABC pada pXO2. Hasil reaksi PCR menghasilkan dua pita DNA berukuran sekitar 600 dan 800 bp pada 20 isolat. Namun, dua isolat lain, masing-masing hanya memiliki salah satu dari kedua ukuran pita DNA tersebut. Sebagian besar koleksi isolat asal Nusa Tenggara dan Papua (91%) masih memiliki kedua plasmid secara lengkap (pXO1+/2+) dan karena itu bersifat virulen, sedangkan dua isolat lain (9%) telah kehilangan salah satu plasmid virulennya sehingga bersifat avirulen. Disimpulkan bahwa PCR multiplex dengan dua pasang primer dengan target amplifikasi pada plasmid dapat digunakan untuk evaluasi status virulensi isolat B. anthraci.

Histopathological Changes of Erythrocyte and Quantity of immature Erythrocytes in Tegal Duckling (Anas Javanica) Induced by Lead (Pb) Hamdani Budiman; Dwinna Aliza
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

The aim of this research is to find out the histopathological changes of erythrocyte and the quantity of immature erythrocyte of tegal duckling treated with lead (Pb). Twenty five young female ducks with the age of 7 days and 60.55.07 g body weight alloted into 4 treatment groups and 1 control group (Ko) with 5 replication each. The treatment group fed with K1 (5.7), K2 (11.4), K3 (17.1), and K4 (22.8) mg Pb/kg body weight for 10 days, respectively. Young ducks showed clinical symptoms such as the color of feces were brown to black, green color of diarrhea, anemic and decreasing body weight. Histopathologically, erythrocyte showed that immature and abnormal in shape (like teardrops, dumb bell, and bottles). The result of variant analysis followed by linier regression shows that the higher the dosage of Pb in diet the higher the quantity of immature erythrocyte andabnormal erythrocyte shape found (P0.01).

IDENTIFIKASI GROWTH DIFFERENTIATION FACTOR-9 (GDF-9) DARI MATURASI IN VITRO OOSIT SAPI DENGAN TEKNIK IMUNOSITOKIMIA Widjiati w; Desi Wulansari; Wurlina w; Ngakan Made Rai Widjaja
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengindentifikasi GDF-9 di dalam oosit yang berasal dari preantral folikel sapi yang dimatangkan secara in vitro. Penelitian ini menggunakan metode imunositokimia ikatan antara molekul avidin dan biotin yang terkandung dalam antibodi sekunder bentuk kompleks avidin-biotin. Ovarium sapi diambil dari rumah potong hewan (RPH) Pegirian Surabaya. Penelitian ini menggunakan 105 oosit matang yang berasal dari 62 ovarium. Hasil imunositokimia menunjukkan bahwa 77,14% GDF-9 teridentifikasi.

MEASUREMENT OF SERUM TESTOSTERONE IN KACANG GOAT BYUSING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUE: THE IMPORTANCE OF KIT VALIDATION (Pengukuran Testosteron Serum Kambing Kacang dengan Teknik Enzyme-Linked Immunosorbent Assay (ELISA): Pentingnya Validasi Kit) Gholib G; Sri Wahyuni; Okta Hilda Kadar; Mulyadi Adam; Triva Murtina Lubis; Azhar A; Muslim Akmal; Tongku Nizwan Siregar; Teuku Armansyah; Taufiq Purna Nugraha
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

This study was conducted to validate a commercial testosterone enzyme-linked immunosorbent assay (ELISA) kits (DRG EIA-1559) inanalytic and biological manner for measuring serum testosterone concentrations in kacang goats. This study used 18 healthy kacang goats, six bucks (2 years), six kids (6 months), and six does (2 years). Blood samples were collected from jugular vein and prepared as serum. Two validation tests were performed, an analytical validation comprises a parallelism, accuracy, precision and sensitivity and a biological validation by comparing testosterone concentration from bucks, kids, and does. Testosterone concentrations were measured using ELISA technique. Data of analytical validation were analyzed descriptively and test of equality of slope was performed to see the parallelism between samples and standard curves. Analysis of variance (ANOVA) was used for biological validation data. Results of parallelism showed that sample curve was parallel to the standard curve. Accuracy, precision (% CV of intra-and inter-assay) and sensitivity of the assay were 99.65±4.27%, 10%, 15% and 0.083 ng/ml, respectively. Results of biological validation showed that the assay used were accurately measured testosterone which testosterone concentrations in bucks were significantly higher compared to kids and does (P0.05). In conclusion, a commercial testosterone ELISA kits (DRG EIA-1559) is a reliable assay for measuring serum testosterone concentration in kacang goats. Key words: analytical and biological validations, ELISA, testosterone, kacang goat

POTENSI SUSPENSI DAN EKSTRAK DAUN KATUK SEBAGAI ANTELMINTIK TERHADAP NEMATODA GASTROINTESTINAL PADA TERNAK KAMBING Razali R; Azhari A; Andi Novita; Teuku Reza Ferasyi; Ridwan R; Ari Munandar
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui potensi ekstrak air dan ekstrak etanol daun katuk (Sauropus androgynus) sebagai antelmintik dalam mengurangi jumlah nematoda gastrointestinal pada ternak kambing. Dalam penelitian ini digunakan 15 ekor kambing kacang jantan lokal berumur 1 tahun dengan bobot badan awal 12,6±1,15 kg. Rancangan yang digunakan dalam penelitian ini adalah rancangan acak lengkap dengan 3 perlakuan dan 5 ulangan. Ketiga perlakuan tersebut adalah P0 (kontrol) hanya diberi akuades; P1, diberikan ekstrak air daun katuk sebanyak 7,44 g/hari; dan P2, diberikan ekstrak etanol daun katuk sejumlah 1,89 g/hari. Perlakuan diberikan per oral sebanyak 2 kali sehari selama 40 hari. Makanan kambing berupa hijauan diberikan 2 kali sehari, sedangkan air minum diberikan ad libitum. Data berat badan dan jumlah nematoda dalam feses dihitung pada hari ke-0, 10, 20, 30, dan 40 pascaperlakuan. Hasil penelitian menunjukkan bahwa pemberian ekstrak air dan ekstrak etanol daun katuk menurunkan jumlah telur nematoda gastrointestinal pada kambing secara signifikan (P

CHARACTERIZATION OF GLYCOCUNJUGATE DISTRIBUTION IN THE EPIDIDYMIS OF THE JAVAN MUNTJACS (Muntiacus muntjak muntjak) Sri Wahyuni; Hamny Sofyan; Hafizuddin Hafizuddin; I Ketut Mudite Adnyane; Gholib Gholib; Muhammad Jalaluddin; Fadli A. Gani; Mulyadi Adam; Tongku Nizwan Siregar; Srihadi Agungpriyono
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

The presence of glycoconjugates in caput, corpus, and cauda epididymis of the Javan muntjacs was performed using lectin histochemical method. The epididymis organs were obtained from two adult male Javan muntjacs in hard antler stage and then immediately fixed in Bouin’s solution and processed in histological manner. Six types of biotinylated lectins namely Peanut agglutinin (PNA), Soybean agglutinin (SBA), Wheat germ agglutinin (WGA), Ricinus communis agglutinin (RCA), Concanavalin A (Con A), and Ulex europaeus agglutinin I (UEA I) were applied to detect the specific glycoconjugates in the epididymal tissue of muntjacs. The result showed the presence of glycoconjugates were detected by five types of lectins except UEA I in caput, corpus, and cauda epididymis with specific distribution and also the intensity of lectins binding. Glycoconjugates β-galactose, β-glucose, mannose, N-acetyl-galactosamine, N-acetyl-glucosamine, and sialic acid were stained with various intensity in epithelial cells, basal cells, stereocilia, spermatozoa, and stroma of caput, corpus, and cauda epididymis. The presence of sialic acid on the surface of spermatozoa detected by WGA are thought to be play a role on sperm protection during their maturation and transit along epididymal lumen. In conclusion, the difference of glycoconjugates distribution pattern and lectin binding in the epididymal caput, corpus, and cauda of the Javan muntjac indicates the regional differences in epididymal function in spermatozoa maturation.

CORRELATION BETWEEN ESTROGEN HORMONE CONCENTRATION AND ESTROUS CYCLE OF RAT FED SOYBEAN FLOUR AND TEMPEH FLOUR Safrida Safrida; Dedy Duryadi Solihin; Mustafa Sabri
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

The objective of this research was to examine the correlation between estrogen level and estrous cycle of rat fed with soybean flour and tempeh flour. A completely randomized design was applied in this study with 6 treatment groups and 5 replications. Rats in group 1, 2, and 3 were non-ovariectomized rats which were fed with pellet, 10 g soybean flour/100 g body weight/day, and 10 g tempeh flour/ 100 g body weight/day, respectively. The rats in group 4, 5, qnd 6 were ovariectomized rat which were fed with pellet, 10 g soybean flour/100 g body weight/day, and 10 g tempeh flour/100 g body weight/day, respectively. The rats were after fed with pellet, soybean and tempeh flour for 4 weeks, the estrous cycle phases of rats were examined based on the presence of vaginal epithelial cells and the number of qualitative vaginal epithelial cells, while the estrogen concentrations in serum were measured by radioimmunoassay method. The data was analyzed using analysis of variance and followed by Duncan multiple range test at 95% confidence. Non-ovariectomized rats given tempeh flour have longer estrus cycle than the non-ovariectomized rats which given soybean flour. The isoflavon contained in tempeh flour and soybean flour induced proliferation and cornification of vaginal epithelial cell. Feeding the soybean flour and tempeh flour to ovariectomized rats could optimize the estrogen hormone in initiating the estrus phase, in which of the tempeh flour addition had better effect than soybean flour. Correlation between the level of estrogen to the length of estrous cycle in both nonovariectomized rats and ovariectomized rats was positive, in which the higher the levels of estrogen the longer the length of estrous cycle.

PROFILE OF ALLERGY HYPERPLASMA PATHOLOGIC ANTIBODY AND IMMUNOGENIC CHARACTERISTIC Nazaruddin Nazaruddin; Hamdani Budiman; Basri A. Gani; Subhaini Jakfar; M. Hasan; Muhammad Hanafiah
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): March
Publisher : Universitas Syiah Kuala

The objective of this research was to determine the allergy hyperplasma pathologic antibody and to evaluate the immunogenic characteristics. Blood and serum were collected from human suffering rhinitis, skin, eye and asthma allergies. To obtain a cloned allergen plasma protein (protein allergen that had been induced with the serum from human with allergic rhinitis, skin, eye and asthma), the goat was used as an intermediate animal. Hematological analysis showed that the leukocyte cell such as neutrophil, lymphocyte, monocyte, and eosinophil increase in allergic-suffered human. The blood smear test exhibited that the mastocyte cell was dominant which contributes to allergy activities in human body. The sodium dodecyl sulphate-polyacrylimide gel electrophoresis (SDS-PAGE) assay detected protein allergens with molecular weight of 188 kDa (IgE) and 60-62 kDa (mastocyte cell). The reactivity assay using enzyme linked immunosorbent-assay (ELISA) revealed that cloned-allergens (whole hyperplasma allergen from goat isolates) express the best reactivity at various concentrations of IgE than the leukocyte cells. This research concluded that the clones of protein allergen have better immunogenic characteristic and those proteins can be recommended as the candidate of allergen to induce the humoral immunity on host and deliver specific product of anti-allergy such as milk.

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Tongku Nizwan Siregar

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Contact Name Tongku Nizwan Siregar

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Editorial Address Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

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Abstract

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Contact Name
Tongku Nizwan Siregar

Contact Email
jkh@usk.ac.id

Phone
+626517551536

Journal Mail Official
jkh@usk.ac.id

Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA