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Jurnal Laboratorium Khatulistiwa
Published by Poltekkes Kemenkes Pontianak
ISSN : 25979523     EISSN : 25979531     DOI : https://doi.org/10.30602/jlk
Core Subject : Health, Science, Education,
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of Medical Laboratory. Jurnal Laboratorium Khatulistiwa particularly focuses on the main problems in the development of the Medical Laboratory health areas as follows: Toxicology Immunoserology Bacteriology Clinical Chemistry Parasitologi Micology And other related disciplines.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Imunologi dan Mikrobiologi (Lain-Lain)
Articles 156 Documents
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Perbedaan Kadar Besi (Fe) pada Air Sumur Gali Di Dusun Wonodadi RT 07/ RW 10 yang Diberi PAC dengan yang Diberi Tawas Mutmainah Kartini; Ratih Indrawati; Suwono Suwono
Jurnal Laboratorium Khatulistiwa Vol 2, No 1 (2018): November 2018
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v2i1.319

Abstract

Abstract: Water is one of the important components in human life. The results of observations by researcher, the Wonodadi Hamlet  RT 07 / RW 10 people using dig well water as clean water source. The dig well water used contains high Fe content, so the people adds PAC and alum (Aluminum Sulfate) before using water to reduce Fe content. The purpose of this research was to analyze the difference of Fe content in dug well water in Wonodadi Hamlet RT 07 / RW 10 which was PAC (Poly Aluminum Chloride) added with alum (Aluminum Sulfate) added. The research design used was quasi experimental research because it did not use the actual research design. The samples were dig well water treated by PAC 100 mg and alum 150 mg adds in every 1000 ml sample. The number of samples in this reserch was determined by replication formula, 16 samples of dig well water were PAC added and 16 samples of dig well water were alum added so that 32 samples were obtained. Samples of dig well water were taken at Wonodadi Hamlet RT 07 / RW 10. Analysis of Fe content in this research using AAS (Atomic Absorbtion Spectrophotometer) method. The average of Fe content in the dig well water added by PAC was 0,9513 ppm. The average of Fe content in dig well water which was added by alum was 1,6735 ppm. Hypothesis in this research is alternative hypothesis (Ha) that there is difference of Fe content on dig well water in Wonodadi Hamlet RT 07 / RW 10 which PAC added with alum added. The result of computerized data processing through Paired T-Test obtained value p = 0,000 (p <0,05). Based on these results, Ha is received means that there is a difference in Fe content dig well water in Wonodadi Hamlet RT 07 / RW 10 which was PAC added with alum added.Abstrak: Air merupakan salah satu komponen penting dalam kehidupan manusia. Hasil observasi yang dilakukan peneliti, masyarakat Dusun Wonodadi RT 07 / RW 10 menggunakan air sumur gali sebagai sumber air bersih. Air sumur gali yang digunakan mengandung kadar Fe tinggi, sehingga masyarakat tersebut menambahkan PAC dan tawas (Aluminium Sulfat) sebelum menggunakan air untuk menurunkan kadar Fe. Tujuan penelitian ini untuk menganalisa perbedaan kadar Fe pada air sumur gali di Dusun Wonodadi RT 07 / RW 10 yang diberi PAC (Poly Aluminium Chloride) dengan yang diberi tawas (Aluminium Sulfat). Desain penelitian yang digunakan adalah penelitian quasi eksperimen karena tidak menggunakan rancangan penelitian yang sebenarnya. Sampel penelitian berupa air sumur gali yang diberi perlakuan dengan menambahkan PAC 100 mg dan tawas 150 mg pada setiap 1000 ml sampel. Jumlah sampel dalam penelitian ini ditentukan dengan rumus replikasi, 16 sampel air sumur gali diberi PAC dan 16 sampel air sumur gali diberi tawas sehingga didapat 32 sampel. Sampel air sumur gali diambil di Dusun Wonodadi RT 07 / RW 10. Analisa penentuan kadar Fe dalam penelitian ini menggunakan metode AAS (Atomic Absorbtion Spectrophotometer). Rata-rata kadar Fe pada air sumur gali yang ditambahkan PAC adalah 0,9513 ppm. Rata-rata kadar Fe pada air sumur gali yang ditambahkan tawas adalah 1,6735 ppm. Hipotesis pada penelitian ini adalah hipotesis alternatif (Ha) yaitu ada perbedaan kadar Fe pada air sumur gali di Dusun Wonodadi RT 07 / RW 10 yang diberi PAC dengan yang diberi tawas. Hasil pengolahan data secara komputerisasi melalui uji Paired T-Test diperoleh nilai p = 0,000 ( p < 0,05). Berdasarkan hasil tersebut, Ha diterima artinya ada perbedaan kadar Fe pada air sumur gali di Dusun Wonodadi RT 07 / RW 10 yang diberi PAC dengan yang diberi tawas.
Penggunaan Antikoagulan Naf pada Pengukuran Kadar Glukosa Darah Selama 2 Jam Etiek Nurhayati; Suwono Suwono; Everiandi Nur Fiki
Jurnal Laboratorium Khatulistiwa Vol 1, No 1 (2017): November 2017
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v1i1.93

Abstract

Abstract: NaF or Sodium Fluoride are anticoagulants which often used for sampling tests of blood glucose levels because NaF is considered to inhibit the process of glycolysis. The purpose of this study was to determine the difference of blood glucose level that delayed for 2 hours using NaF anticoagulant and without using NaF anticoagulant at students of Medical Laboratory Pontianak. This research method was in the form of Cross-Sectional research. Sampling technique by random sampling with 39 people in total. Glucose examination method used was a method of glucose oxidase using blood serum. The result was the average value of blood glucose level using anticoagulant NaF was 80,256 mg/dl. While the average of blood glucose levels without the use of NaF anticoagulants was 73,589 mg/dl. The data obtained were analyzed statistically using paired t-test result (p = 0,001 <a 0,05). It can be concluded that there was a difference of blood glucose level when using NaF anticoagulant and without using NaF anticoagulant on students of Medical Laboratory Pontianak.Abstrak: Antikoagulan NaF atau Natrium Flourida adalah antikoagulan yang sering digunakan untuk sampling bahan pemeriksaan kadar glukosa darah, karena NaF dianggap mampu menghambat proses glikolisis. Tujuan penelitian ini untuk mengetahui perbedaan kadar glukosa darah sewaktu yang ditunda selama 2 jam menggunakan antikoagulan NaF dan tanpa menggunakan antikoagulan NaF pada mahasiswa/I Analis Kesehatan Pontianak. Desain penelitian menggunakana Cross Sectional. Teknik pengambilan sampel dengan cara simple random sampling dengan jumlah 39 orang. Metode pemeriksaan glukosa yang digunakan adalah metode glucose oxidase menggunakan sthe erum darah Hasil penelitian adalah rata–rata nilai kadar glukosa darah yang menggunakan antikoagulan NaF adalah 80,256 mg/dl. Sedangkan rata-rata kadar glukosa darah yang tanpa menggunakan antikoagulan NaF adalah 73,589 mg/dl. Data yang diperoleh dianalisis statistik menggunakan uji t-berpasangan didapatkan hasil (p = 0,001<a 0,05). Dapat disimpulkan terdapat perbedaan kadar glukosa darah sewaktu menggunakan antikoagulan NaF dan tanpa menggunakan antikoagulan NaF pada mahasiswa /i Analis Kesehatan Pontianak. 
Potensi Air Perasan Kunyit Putih, Kunyit Kuning Dan Kunyit Hitam Terhadap Bakteri Escherichia coli Penyebab Diare Sari, Emilda; Suwandi, Edy; Triana, Linda; Nurhidayattulloh, Ariffialdi
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1392

Abstract

Turmeric is a herbal plant that is widely used by the public (Curcuma domestica Val) which is proven to contain ingredients that can function as an antibacterial. The antibacterial properties of turmeric rhizomes are caused by its main chemical content, namely curcuminoids and essential oils. Other active substances in turmeric that can be used as antibacterials are flavonoids, alkaloids and tannins. This compound functions as an antibacterial.This research aims to explain the differences in the inhibitory power of White Turmeric, Yellow Turmeric and Black Turmeric juice against Escherichia coli bacteria. The research design used was quasi-experimental. The samples in this study were the juice of White Turmeric, Yellow Turmeric and Black Turmeric which was repeated 10 times for each treatment.Based on the research results, it is known that white turmeric rhizome juice has an average barrier potential of 33.7 mm against Escherichia coli bacteria. This is no different from the juice of Yellow Turmeric rhizomes which has an average barrier potential of 32.7 mm against Escherichia coli bacteria. Meanwhile, the juice from Black Turmeric has a smaller potential resistance compared to the juice from the rhizomes of White Turmeric and Yellow Turmeric, namely an average of 26.2 mm.
Produksi Komponen Darah Packed-Red Cell (PRC), Liquid Plasma (LP), Thombocytes Concentrates (TC) dan Fresh-Frozen-Plasma (FFP) di UDD PMI Kabupaten Bojonegoro Muflikhah, Nina Difla; Nuraini, Fatia Rizki; Anggita, Etri Sukma
Jurnal Laboratorium Khatulistiwa Vol 7, No 1 (2023): NOVEMBER 2023
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i1.1163

Abstract

The processing of blood components is an act of separating the components of donor blood through a process by taking into account the quality and safety of blood component products. Its process produce various type of blood component, such as Packed Red Cell, Liquid Plasma, Thrombocytes Concentrate and Fresh Frozen Plasma. The quantity of each blood production depends on the mount of demant from hospital to threat the patient for transfusion. Indonesia Red Cross especially in Blood Donor Unit of each region have a important duty to collect and process the whole blood component as much as it needed. This type of research is a descriptive study with a cross sectional approach. The population used was the production of PRC, LP, TC and FFP  at UDD PMI Bojonegoro from June to July 2022 as many as 2124 kolf. The sampling technique used purposive sampling. Data analysis using univariate analysis which is presented in the form of a frequency distribution table and ghraphic for daily production. Production of blood components as much as 2124 kolf, Production of PRC as 984 kolf (46%), LP as 904 kolf (43%0, TC as 200 kolf (9%), and FFP as 36 kolf (2%). The highest blood component production is PRC, followed by LP, TC and FFP, respectfully.  
Identifikasi Bakteri Coliform pada Minuman Air Tahu dan Air Tebu yang Dijual di Wilayah Kota Pontianak Ramadan, Aditiya; Jordan, Aristo; Ardhani, Adha Riesta; Monalita, Ria; Munardi, Fhatia Natasya; Syahdilla, Ade; Nuswantoro, Ari; Triana, Linda
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1470

Abstract

Air tahu dan air tebu merupakan minuman manis dan menyegarakan yang cukup diminati oleh masyarakat Kota Pontianak terutama dari kalangan anak-anak, remaja, dan orang tua. Cemaran bakteri Coliform yang terdapat pada minuman air tahu dan air tebu yang dijual di Kota Pontianak dapat menyebabkan masalah serius pada kesehatan. Tujuan dari penelitian ini adalah untuk mengetahui adanya cemaran bakteri Coliform pada minuman air tahu dan air tebu yang dijual di pinggir jalan Kota Pontianak dengan menggunakan metode kultur. Desain penelitian ini adalah penelitian deskriptif dengan pengambilan sampel secara total sampling. Penelitian ini dilakukan dengan cara mengambil sebanyak 43 sampel air tahu dan 70 sampel air tebu yang dijual di pinggir jalan di Kota Pontianak. Hasil penelitian dari 43 sampel air tahu menunjukkan 17 sampel (39,5%) positif bakteri Coliform, 26 sampel lainnya (60,5%) negatif bakteri Coliform, dan hasil peneilitan dari 70 sampel air tebu menunjukkan 37 sampel (52,8%) positif bakteri Coliform, dan 33 sampel lainnya (47,2%) negatif Coliform.
Perbedaan Kadar Formalin Pada Ikan Asin Gabus Sebelum Dan Sesudah Perendaman Triana, Linda; Wahdaniah, Wahdaniah; Sari, Emilda
Jurnal Laboratorium Khatulistiwa Vol 7, No 1 (2023): NOVEMBER 2023
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i1.1256

Abstract

Maraknya penggunaan bahan tambahan pangan berbahaya yaitu formalin pada ikan asin dikarenakan formalin merupakan zat pengawet yang mudah didapatkan dengan harga yang murah. Pada ikan asin penggunaan formalin selain bertujuan untuk pengawetan agar ikan asin  tidak ditumbuhi jamur. Penelitian ini dilakukan untuk menganalisis perbedaan kadar formalin pada ikan asin gabus berformalin sebelum dan sesudah perendaman pada suhu 20°C, 70°C, dan 100°C. Penelitian ini menggunakan desain eksperimental (experiment), sampel yang digunakan adalah ikan asin gabus yang mengandung formalin. Jumlah pengulangan setiap perlakuan sebanyak enam kali dengan jumlah sampel sebanyak 24 sampel. Hasil penelitian rata-rata kadar formalin pada ikan asin gabus berformalin 3,14 mg/kg, setelah direndam selama 5 menit pada suhu 20ºC, 70°C dan 100°C secara berturut-turut adalah 3,01 mg/kg, 2,11 mg/kg, dan 1,68 mg/kg, penurunan kadar formalin sebesar 4,14%; 32,80% dan 46,50%. Berdasarkan hasil uji Friedman didapatkan hasil p = 0,000 < 0,05 maka dapat diketahui ada perbedaan kadar formalin pada ikan asin gabus berformalin sebelum dan sesudah perendaman pada suhu 20°C, 70°C, 100°C.
Proteksi Biji Kopi Dalam Memperbaiki Morfologi Sperma Pada Tikus Yang Diinduksi Streptozotocin Maulana, Mochamad Rizal; Sofyanita, Eko Naning
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1421

Abstract

Pemberian induksi Streptozotocin (Stz) dapat menurunkan kualitas sperma salah satunya morfologi sel spermatozoa, kopi mempunyai kandungan antikosidan yang tinggi yaitu asam klorogenat yang merupakan antioksidan kuat, mampu bertindak sebagai antioksidan eksogen yang melawan ROS dalam sel. Tujuan dari penelitian ini untuk mengatahui pengaruh pemberian kopi pada tikus yang diinduksi Stz. Post Test Control Group Design dengan sampel 24 tikus jantan Rattus norvegicus umur 3 bulan, berat badan ± 250 gram, dibagi menjadi 4 kelompok secara random. Setelah 1 minggu aklimasi, pengobatan diberikan 1 kali sehari selama 14 hari. Hari ke 15 dilakukan pemerkksaan morfologi sperma.. Hasil penelitian morfologi spermatozoa (%) pada kelompok KN (50,46) kelompok KP (64,58) kelompok KK1 (59,50) dan kelompok KK2 (72,05) berbeda artinya, p< 0,05. Kesimpulan pada penelitian ini yaitu Pemberian kopi mampu memperbaiki morfologi spermatozoa pada tikus yang diinduksi Stz.
FORMULASI SEDIAAN SPRAY HAND SANITIZER PERASAN KULIT NANAS (Ananas comosus L. Merr) DALAM MENGHAMBAT PERTUMBUHAN BAKTERI Escherichia coli SECARA IN VITRO Slamet, Slamet; Budi Sungkawa, Hendra; Dea Sari, Khafifah
Jurnal Laboratorium Khatulistiwa Vol 7, No 1 (2023): NOVEMBER 2023
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i1.1211

Abstract

Pineapple peel has flavonoid compounds that are disinfectant and very effective in inhibiting the growth of gram-positive and gram-negative bacteria. This is because flavonoids are polar so it is easier to penetrate the peptidoglycan layer. Pineapple peel has a high inhibitory power against Escherichia coli and Staphylococcus aureus bacteria. This study aims to determine the effect of pineapple peel (Ananas comosus L. Merr) hand sanitizer spray preparation in inhibiting the growth of Escherichia coli bacteria in vitro. The design of this research is experimental research. The method used was disc diffusion. The population in this study is pineapple peel juice. The sampling technique used was purposive sampling. Based on the results of the study of 6 concentrations of pineapple peel juice hand sanitizer, the lowest inhibition zone was 6 mm at a concentration of 50% and the highest at a concentration of 100%, namely a 10 mm inhibition zone. From the results of the organoleptical test examination of the pineapple peel juice hand sanitizer spray preparation, which is liquid, light yellow in color, slightly cloudy and has a distinctive smell of perfume. pH is 6 at 50% 60% 70% 80% concentration and 5 at 90% 100%. The irritation test obtained negative results so that the product is safe for use on the skin. Homogeneity test is not homogeneous. Based on the results of the examination, the results of the organoleptical test, pH test, and irritation test meet the requirements but the homogeneity test does not meet the requirements in the aesthetics of spray hand sanitizer products.
UJI AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL BIJI NANGKA (Artocarpus heterophyllus Lam.) TERHADAP STABILISASI MEMBRAN SEL DARAH MERAH Wahdaniah, Wahdaniah; Sabrina Azani, Aulia; Kamilla, Laila
Jurnal Laboratorium Khatulistiwa Vol 7, No 1 (2023): NOVEMBER 2023
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i1.1254

Abstract

Jackfruit seed (Artocarpus heterophyllus Lam.) is local plant that contains compounds such as flavonoids, saponins and steroids that have potential as antiinflammatory. This research aims to determine the difference in antiinflammatory activity of ethanol extract jackfruit seed (Artocarpus heterophyllus Lam.) from sodium diclofenac on red blood cell membrane stability. Antiinflammatory activity can be seen from the decrease in absorbance of hemoglobin lysed in test solution after being induced by hypotonic solution and compared with the positive control (sodium diclofenac). This research is a quasi experiment with the method of red blood cell membrane stability in vitro using purposive sampling technique with samples of ethanol extract jackfruit seed concentrations of 0,005%, 0,01%, 0,02% and 0,04% which were repeated 3 times. The results showed that ethanol extract jackfruit seed (Artocarpus heterophyllus Lam.) has the ability to stabilize red blood cell membrane with a percent stability at concentration of 0,005% is 41,70%, concentration of 0,01% is 50,70%, concentration of 0,02% is 54,20% and concentration of 0,04% is 64,60%. The antiinflammatory activity of ethanol extract jackfruit seed 0,02% is not different from sodium diclofenac 0,005% with p value > 0,05, whereas ethanol extract jackfruit seed 0,005%, 0,01% and 0,04% are different from sodium diclofenac.
HUBUNGAN SEKS BEBAS DENGAN KEJADIAN SIFILIS DI WILAYAH KERJA PUSKEMAS KOM YOS SUDARSO KOTA PONTIANAK Dj, Herlinda Djohan; Sutriswanto, Sutriswanto; Nurhayati, Etiek; Ratika, Meila; Salim, Maulidiyah
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1347

Abstract

Syphilis, also known as "the lion king", is an STI (Sexually Transmitted Infection), a disease caused by infection with the Treponema Pallidum bacteria. It is chronic and chronic. This is a dangerous disease because it can attack all organs of the body. This research was conducted at the Kom Yos Sudarso Pontianak Community Health Center because the Community Health Center is a referral health center for syphilis examinations and the respondents were people who were caught by field officers who allowed free sex.               This study aims to find out who suffers from syphilis among respondents who engage in casual sex, as well as finding out the percentage of syphilis incidents in people who engage in casual sex and to find out the relationship between casual sex and the incidence of syphilis in the work area of the Kom Yos Sudarso Community Health Center, Pontianak City.               This research design uses cross sectional with random sampling technique. The samples used were 123 patient blood samples using the TP Rapid method.               Based on the results of the research conducted, casual sex and the incidence of syphilis were obtained. The results of analysis using the chi square test using Continuity Correctionb obtained a value of p = 0.021 < 0.05, so HA was accepted so that there was a significant relationship between casual sex and the incidence of syphilis in the work area of the Community Health Center. Yos Sudarso, Pontianak City.

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