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Jurnal Laboratorium Khatulistiwa
Published by Poltekkes Kemenkes Pontianak
ISSN : 25979523     EISSN : 25979531     DOI : https://doi.org/10.30602/jlk
Core Subject : Health, Science, Education,
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of Medical Laboratory. Jurnal Laboratorium Khatulistiwa particularly focuses on the main problems in the development of the Medical Laboratory health areas as follows: Toxicology Immunoserology Bacteriology Clinical Chemistry Parasitologi Micology And other related disciplines.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Imunologi dan Mikrobiologi (Lain-Lain)
Articles 156 Documents
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Gagal Ginjal Kronik Yang Menjalani Terapi Hemodialisa Di RSUD dr ABDUL AZIZ Nurhayati, Etiek; Kamilla, Laila; Lestari, cik Verra Budi
Jurnal Laboratorium Khatulistiwa Vol 7, No 1 (2023): NOVEMBER 2023
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i1.1246

Abstract

Hypertension is when a person experiences increased blood pressure on tissue and organ perfusion. One complication that can be caused by hypertension is kidney failure. Urea and creatinine are waste products of the body's metabolism. Ureum and creatinine levels will increase, indicating impaired kidney function. Damage that occurs to the kidneys must be treated one way with hemodialysis. The function of hemodialysis therapy is to reduce urea and creatinine levels in patients with chronic kidney failure.The study's general objective was to analyze urea and creatinine levels in hypertensive patients with chronic kidney failure undergoing hemodialysis therapy at dr. Abdul Aziz Singkawang General Hospital. The research design is analytically observational with a cross-sectional research design. Secondary data in this study were obtained from medical records and laboratory results of hypertensive patients with chronic kidney failure undergoing hemodialysis therapy without complications of DM, namely 37 respondents who performed urea and creatinine examinations before and after hemodialysis at dr. Abdul Aziz Singkawang by using a purposive sampling technique.The results of the study found 64 hypertensive patients undergoing hemodialysis and obtained a sample of 37 patients who met the criteria, namely hypertensive patients undergoing hemodialysis without complications of DM got an average urea level before hemodialysis 187.1919 mg/dl and after hemodialysis 136.0162 mg/dl. The intermediate creatinine level before hemodialysis was 14.0870 mg/dl and 11.0224 mg/dl after hemodialysis.The results of the Wilcoxon Signed Rank Test. They obtained p-value = 0.000 <α (0.05), which indicated differences in urea and creatinine levels before and after hemodialysis therapy in hypertensive patients with chronic kidney failure.
EFEKTIFITAS KOMBUCHA TEH BUNGA ROSELLA DALAM MENGHAMBAT PERTUMBUHAN BAKTERI ESHERICHIA COLI DAN SHIGELLA DYSENTERIAE UNTUK MENCEGAH TERJADINYA DIARE Fatayati, Imma; Djohan, Herlinda; Roofiif, Sayyid Al
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1348

Abstract

One probiotic product is kombucha tea. Kombucha tea can be combined with rosella tea. Some of the bacteria that attack the digestive tract are Escherichia Coli and Shigella dysenteriae which can cause diarrhea. It has been suggested that probiotics can be used to prevent diarrhea. This research aims to determine whether there are probiotic benefits of Rosella flower kombucha fermented for 7 days and 14 days to prevent diarrhea. Review of the growth inhibitory power of Escherichia Coli and Shigella dysenteriae bacteria. This research is in the form of Quasi-experimental. The population of this study was rosella flower kombucha. The sample for this research was rosella flower kombucha with 7 days of fermentation and 14 days of fermentation. The number of repetitions for each treatment was 16 replications, with total replications was 62 replications. The results of this research showed that the inhibitory power of rosella flower kombucha against Shigella dysenteriae bacteria with an average fermentation time of 7 days was 11.31 mm (strong), for 14 days of fermentation the average was 24.72 mm (very strong). Meanwhile, the inhibitory power of rosella flower kombucha against E. Coli bacteria with a fermentation time of 7 days averaged 11.09 mm (strong), for 14-day fermentation, the average was 20.34 mm (very strong). This research concluded that the inhibitory power of rosella flower kombucha fermented for 7 days was in the strong category and 14 days in the very strong category for both the growth of Shigella dysentriae and `E. coli bacteria.
PERBEDAAN MODIFIKASI LARUTAN ZnS (Zinc sulfite) DENGAN NaCl (Natrium Cloride) 0.9 % METODE FLOTASI TERHADAP JUMLAH TELUR CACING Soil Transmitted Helminth (STH) Siregar, Saadah; Manurung, Arnita Elisabet; Rizky, Vincentia Ade; Krisdianilo, Vinsensius
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1405

Abstract

Worms is a disease caused by the entry of parasites in the form of worms into the human body due to the influence of environmental factors or food that is not kept clean. Factors that cause the risk of helminthiasis in humans include food that is infected with worm eggs, feet that are in direct contact with soil containing worm vectors because they do not wear footwear, the habit of defecating in random places, availability of clean water, hand washing, washing habits. plates, And nail hygiene. The purpose of this study was to determine the difference in the number of worm eggs to be found using ZnS 1% and NaCl 0.9% solution using the flotation method. The data obtained from faecal examination using the flotation method with ZnS 1% and NaCl 0.9%, namely Soil Transmitted Helminth (STH) worm eggs, then the number of eggs will be presented in table form and analyzed descriptively. Result With the average yield of worm eggs found in  a solution of ZnS  1% and NaCl 0.9% using the flotation method for the number of Soil Transmitted Helminth (STH) worm eggs. In ZnS 1% solution the average is 2.12 with 4 Ascaris lumbricoides eggs, Trichuris trichura 2 and 3 hookworms while in 0.9% NaCl solution the average is 1.31 with 5 Ascaris lumbricoides worm eggs, Trichuris trichura 1 and worms mine 1. The Manny Whitney statistical test showed results of Sig 0.003 (P<0.005), so it was concluded that there was a difference in the number of STH worm eggs found using ZnS 1% solution and 0.9% NaCl using the flotation method.
UJI EFEKTIVITAS MINYAK ATSIRI DAUN KEMANGI (Ocimum sanctum Linnaeus) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus Sutriswanto, Sutriswanto; Kurniawati, Fifi; Sulistiyowati, Endah; Syopingi, Syopingi
Jurnal Laboratorium Khatulistiwa Vol 7, No 1 (2023): NOVEMBER 2023
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i1.1245

Abstract

Herbal plants are plants that have a function in health as medicine. There are lots of plants that can be used as herbal medicine, one of which is the basil plant. Basil leaves contain chemical compounds including saponins, flavoniods, tannins and essential oils. The main ingredient in basil is essential oil. The essential oil of basil has the potential as an antibacterial. The purpose of this study was to analyze the effectiveness of basil leaf essential oil in inhibiting the growth of Staphylococcus aureus bacteria based on the standard antibiotic inhibition zone according to CLSI. This study used a quasi-experimental research design (Quasy Experiment) with a purposive sampling technique. The population of this research is basil (Ocimum sanctum Linnaeus). The samples used were basil essential oil concentrations of 30%, 32%, 34%, 36%, 38%, 40%, 42%, 44% and 46%. Then tested the antibacterial activity using the diffusion method. Based on research the average inhibition zone at a concentration of 30% inhibition zone formed was 15 mm, 32% concentration was 18 mm, 34% concentration was 16.67 mm, 36% concentration was 15.67 mm, 38% concentration was 17.67 mm, 40% concentration was 22.33 mm, 42% concentration was 16.33 mm, 44% concentration was 18 mm, 46% concentration of 21.33 mm. The conclusion in this study is that there is effectiveness of basil leaf essential oil with the interpretation of Sensitive results in inhibiting the growth of Staphylococcus aureus bacteria by the diffusion method based on the standard antibiotic inhibition zone according to CLSI.
PENGARUH ANTIBAKTERI SEDIAAN SABUN WAJAH CAIR EKSTRAK ETANOL DAUN BELIMBING WULUH (Averrhoa bilimbi L) TERHADAP Staphylococcus aureus METODE DIFUSI Tumpuk, Sri; Suriansih, Suriansih; Nurhayati, Etiek
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1391

Abstract

Facial soap used to clean the face from exposure to dust, pollution, dirt, reduce sebum on the face and eradicate bacteria that can cause acne. Star fruit leaves are efficacious in healing several diseases, such as purulent skin inflammation, boils and medicine for gout. Phytochemical of star fruit leaves contain alkaloid, flavonoid, saponin, tannin and steroid compounds that can inhibit bacterial growth. This study aims to determine the effect of liquid facial soap containing of star fruit leaf extract (Averrhoa bilimbi L) on the growth of Staphylococcus aureus bacteria by diffusion method.This study used a Quasi Experiment research design. The population in this study were wuluh star fruit leaves. The sample used is star fruit leaf extract which is made into liquid facial soap preparations with concentrations of 5%, 10%, 15% and 20%. This antibacterial testing method uses the Kirby-Bauer diffusion method. The results of antibacterial research obtained the average diameter of the inhibition zone at 5% concentration of 10.60 mm, 10% concentration of 11.20 mm, 15% concentration of 12.10 mm, and 20% concentration of 13.23 mm. Based on the diameter of the inhibition zone formed at concentration of 5% is categorized as a moderate response and at concentration of 10%, 15% and 20% is categorized as a strong response.The results of simple linear regression statistical analysis obtained p value = 0.000 < α 0.05 which means Ha is accepted, which means that there is a significant effect on the preparation liquid facial soap ethanol extract of star fruit leaves (Averrhoa bilimbi L) against the growth of Staphylococcus aureus diffusion method.
UJI AKTIVITAS ANTI BAKTERI SEDIAAN SPRAY DAUN KEMANGI (Ocimum sanctum L.) TERHADAP PERTUMBUHAN staphylococcus epidermdis Dj, Herlinda Djohan; Ratnawati, Gervacia Jenny; Sugito, Sugito; D.M.T, Immaculata
Jurnal Laboratorium Khatulistiwa Vol 7, No 1 (2023): NOVEMBER 2023
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i1.1207

Abstract

Daun kemangi merupakan salah satu tanaman yang memiliki banyak manfaat salah satunya adalah sebagai anti bakteri. Daun kemangi memiliki senyawa kimia seperti flavonoid, tanin, fenolik, saponin dan minyak atsiri yang dapat berperan sebagai anti bakteri. Sehingga dapat digunakan untuk kesehatan dan  kebersihan kaki, terutama untuk penghilang bau kaki yang disebabkan oleh bakteri. Tujuan dari penelitian ini adalah untuk mengetahui adanya aktivitas anti bakteri pada sediaan spray perasan daun kemangi dalam menghambat pertumbuhan bakteri staphylococcus epidermidis. Metode uji yang digunakan ialah metode difusi cakram. Perasan daun kemangi diformulasikan menjadi spray dengan konsentrasi yang berbeda yaitu 15%, 20% dan 25% setiap konsentrasi dilakukan 9 kali pengulangan sehingga sampel yang digunakan sebanyak 27 sampel. Sediaan spray kemudian di uji evaluasi dan di uji aktivitas anti bakteri terhadap bakteri staphylococcus epidermidis. Hasil penelitian menunjukkan bahwa sediaan spray perasan daun kemangi memiliki aktivitas anti bakteri dalam menghambat pertumbuhan staphylococcus epidermidis dengan luas zona hambat berturut-turut sebesar 9,61 mm (F1=15%) kategori sedang, 10,56 mm (F2=20%) kategori sedang, 12,83 mm (F3=25%) kategori kuat.
Gambaran Jumlah Leukosit Pada Penderita Suspek So TB Paru di Rumah Sakit Patuh Patut Patju Reginata, Ni Luh Vira; Rohmi, Rohmi; Manu, Thomas Tandi; Khusuma, Ari
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1453

Abstract

Leukocyte examination is carried out to describe disease events or processes in the body, aspecially infectious diseases such as tuberculosis bacteria can attack immune system cells, including leukocytes. Bacteria can survive and reproduce in leukocytes. Pulmonary TB sufferers before treatment saw an increases in leukocyte levels, indicating the formation of many leukocytes to fight bacteria in the phatocytosis process. On of the laboratory tests used to support the diagnosis of tuberculosis is an examination of the leukocyte count. This study aimed to determine the description of the number of leukocytes in patients with Suspected SO Pulmonary TB at The Patuh Patut Patju Hospital Untuk. This study design is descriptive case study research. Sampling technique was carried out using accidental sampling technique and  30 respondents obtained. The results of this study showed that the average leukocyte levels of patients with suspected SO Pulmonary TB were 9,643/μl, with 13 people (1+), 12 people (2+), 5 people (3+). The highest average leukocyte count was found in the female gender, age group 56-65 years and on 5 months treatment
Efektivitas Sediaan Sabun Wajah Cair Ekstrak Daun Katuk (Sauropus androgynus (L.) Merr.) terhadap Pertumbuhan Bakteri Staphylococcus aureus Metode Difusi Salim, Maulidiyah; Gestiwana, Oksa; Kamilla, Laila
Jurnal Laboratorium Khatulistiwa Vol 7, No 1 (2023): NOVEMBER 2023
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i1.1255

Abstract

Sweet leaf bush are one of the vegetable plants that are rich in nutrients and secondary metabolites, so they are commonly used as vegetables and as herbal medicines. The results of the phytochemical screening of sweet leaf bush contain tannins, saponins, flavonoids and alkaloids, so that sweet leaf bush can be used as an antibacterial liquid facial soap preparation. This study aims to determine the effectiveness of liquid facial soap preparations of sweet leaf bush extract formula I with a concentration of 5%, formula II with a concentration of 10%, formula III with a concentration of 15% and formula IV with a concentration of 20% in inhibiting the growth of Staphylococcus aureus bacteria using the diffusion method.This research design uses Quasi Experiment. The population in this research is sweet leaf bush. The sample used was sweet leaf bush extract which was used to make antibacterial liquid facial soap preparations with formula I of 5% concentration, formula II with a concentration of 10%, formula III with a concentration of 15% and formula IV with a concentration of 20%. Antibacterial testing method using the Kirby-Bauer diffusion method.The results of the antibacterial study obtained an average diameter of the inhibition zone in formula I of 12,167 mm, formula II of 14,667 mm, formula III of 17,083 mm and formula IV of 18,667 mm. Based on the diameter of the inhibition zone formed in formulas I, II, III and IV it is categorized as a strong inhibition response.The results of the statistical analysis of the One Way Anova test obtained p value = 0.000 < α 0,05, which means that there is a difference between the average concentrations of the sweet leaf bush extract liquid face soap formula in inhibiting the growth of Staphylococcus aureus bacteria using the diffusion method.Sweet leaf bush are one of the vegetable plants that are rich in nutrients and secondary metabolites, so they are commonly used as vegetables and as herbal medicines. The results of the phytochemical screening of sweet leaf bush contain tannins, saponins, flavonoids and alkaloids, so that sweet leaf bush can be used as an antibacterial liquid facial soap preparation. This study aims to determine the effectiveness of liquid facial soap preparations of sweet leaf bush extract formula I with a concentration of 5%, formula II with a concentration of 10%, formula III with a concentration of 15% and formula IV with a concentration of 20% in inhibiting the growth of Staphylococcus aureus bacteria using the diffusion method.This research design uses Quasi Experiment. The population in this research is sweet leaf bush. The sample used was sweet leaf bush extract which was used to make antibacterial liquid facial soap preparations with formula I of 5% concentration, formula II with a concentration of 10%, formula III with a concentration of 15% and formula IV with a concentration of 20%. Antibacterial testing method using the Kirby-Bauer diffusion method.The results of the antibacterial study obtained an average diameter of the inhibition zone in formula I of 12,167 mm, formula II of 14,667 mm, formula III of 17,083 mm and formula IV of 18,667 mm. Based on the diameter of the inhibition zone formed in formulas I, II, III and IV it is categorized as a strong inhibition response.The results of the statistical analysis of the One Way Anova test obtained p value = 0.000 < α 0,05, which means that there is a difference between the average concentrations of the sweet leaf bush extract liquid face soap formula in inhibiting the growth of Staphylococcus aureus bacteria using the diffusion method.
Perbedaan Proporsi Sputum Bakteri Tahan Asam Positif pada Pasien Diabetes Melitus Terkendali dan Tidak Terkendali Harahap, Elga Citra Lylyanti; Purwanti, Angki; Hardianto, Nova
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1590

Abstract

Currently, when the prevalence of diabetes mellitus (DM) is high and predicted to continue to increase, the elimination of tuberculosis (TB) in Indonesia has not been successful. The challenge is greater due to the comorbidity of both diseases and the complexity of the relationship between the two that is not yet well understood. Therefore, studies on DM-TB comorbidity in various populations are still needed. This study aims to determine the difference in the proportion of positive acid-fast bacillus (AFB) sputum between groups of controlled DM patients (HbA1c <6.5%) and uncontrolled (HbA1c ≥6.5%). The design of this study is cross-sectional, using secondary data (medical records) of 126 DM patients at the Persahabatan Hospital, Jakarta, who underwent examinations in January-June 2024. The results showed that the proportion of positive AFB sputum in the uncontrolled DM patient group was 41.3 percent and in the controlled group was 11.1 percent (based on the Chi-Square test, p-value <0.0001). In addition, the uncontrolled DM patient group had a median (youngest-oldest) age of 55 (37-74) years, while the controlled DM group was 50 (32-70) years (based on the Mann-Whitney test, p-value 0.036). This study shows that the uncontrolled DM patient group had a higher proportion of positive BTA sputum and was significantly older than the controlled DM patient group.
Analisa Kadar Timbal Dalam Darah Dengan Kadar Hemaglobin Dan Hematokrit Pada Supir di Kota Palembang Lubis, Agnes Felicia; Ch, Lidwina Septie
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1567

Abstract

Palembang City is a major urban center located on the island of Sumatra that has experienced substantial growth and development. The rapid increase in population and industrial activity has led to widespread contamination of water, food, and beverages with heavy metals. This is worsened by other variables, including smoking habits and work-related issues. At now, there is a limited number of papers that examine blood as a biomarker for lead contamination, particularly in Palembang City. These articles mostly focus on study participants that are very likely to be exposed, such as drivers. The objective of this study was to evaluate the blood lead levels in a cohort of drivers in Palembang City as an indicator of lead pollution. The participants in this study were drivers, with an average age of 58 years, who had accumulated more than 20 years of work experience (average 28 years). Utilizing the APHA technique, quantifying blood lead concentrations, and employing the impedance method and Spearman test to establish a correlation between the results, evaluating hemoglobin and hematocrit levels. The research findings indicate a correlation between blood lead levels and hemoglobin values (significance: 0.037) as well as hematocrit (significance: 0.039). The mean blood lead concentrations in drivers remain below the threshold of 14.9804 µg/L. The investigation conducted indicates a clear association between the levels of lead in the blood and the values of hemoglobin and hematocrit in the blood of drivers in Palembang City. A strong association was seen between blood lead levels and hemoglobin and hematocrit values

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