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Jurnal Laboratorium Khatulistiwa
Published by Poltekkes Kemenkes Pontianak
ISSN : 25979523     EISSN : 25979531     DOI : https://doi.org/10.30602/jlk
Core Subject : Health, Science, Education,
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of Medical Laboratory. Jurnal Laboratorium Khatulistiwa particularly focuses on the main problems in the development of the Medical Laboratory health areas as follows: Toxicology Immunoserology Bacteriology Clinical Chemistry Parasitologi Micology And other related disciplines.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Imunologi dan Mikrobiologi (Lain-Lain)
Articles 156 Documents
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Analisis Kadar Asam Asetat (CH3COOH) Dalam Cuka Rambai Yang Difermentasi Pada Industri Rumahan Di Desa Sungai Pangkalan Kecamatan Sungai Raya Triana, Linda; Sari, Emilda
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1669

Abstract

Rambai fruit (Baccaurea motleyana) is a fruit that is still unable to compete with other local fruits, so it has lower economic value. Not only is it eaten directly, but rambai fruit can also be processed into vinegar. Vinegar is a substance made from various sugary or starchy ingredients through alcoholic fermentation followed by acetic fermentation. The vinegar sold must contain at least 4% (4 grams of acetic acid per 100 ml), must be fresh, and be made from fruit that is suitable for consumption. The vinegar that is widely available on the market and consumed by the public is made from chemicals that are harmful to the body. For this reason, it is necessary to make natural innovations in making vinegar, for example by using fruit. This research aims to determine the levels of acetic acid (CH3COOH) in rambai vinegar fermented for 1, 3, 12, and 36 months. The research method uses a descriptive research design. The sample used was rambai vinegar produced from a home industry in Sungai Pangkalan Village, Sungai Raya District, fermented for 1, 3, 12 and 36 months. Titrimetric examination method. Research on acetic acid levels in rambia fruit vinegar fermented for 1, 3, 12, and 36 months found 1.5802%, 1.5848%, 1.5917%, and 1.6991%. The conclusion was that the acetic acid content in Zambia fruit vinegar fermented for 1, 3, 12, and 36 days was still below the Indonesian national standard, namely 4-12%.
IDENTIFIKASI INFEKSI CACING GOLONGAN Soil Transmitted Helminths (STHs) PADA ANAK USIA SEKOLAH DASAR DI DESA TALAWAAN ATAS KECAMATAN WORI Lalangpuling, Indra Elisabet; Ganing, Nauvila Salsabila; Katiandagho, Dismo; Suwarja, Suwarja
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1541

Abstract

Helminthiasis remains a public health problem in Indonesia, particularly among elementary school-aged children. This is due to geographical factors, socioeconomic conditions, and behaviors that neglect hygiene. Infection with Soil-Transmitted Helminths (STHs) can have detrimental effects on children's health and development. This study aimed to determine the prevalence of STH infections among elementary school children in Talawaan Atas Village, Wori District, North Minahasa Regency. This study employed a descriptive method. Samples were examined using both native and Kato-Katz methods. The study sample consisted of 30 elementary school students from GMIM Talawaan Atas who met the inclusion and exclusion criteria. Data was collected through fecal examination and questionnaires. The results showed that of the 30 samples examined using the native method, 3 samples (10%) were found to be infected with STHs, specifically Ascaris lumbricoides. Using the Kato-Katz method, the same 1 sample (3.3%) was found to be infected with Ascaris lumbricoides. The conclusion of this study is that there is a 10% prevalence of STH infections among elementary school children in Talawaan Atas Village, Wori District, North Minahasa Regency.
Deteksi Jamur Malassezia spp. Penyebab Infeksi Jamur Kulit Pada Santri Pondok Pesantren Kota Denpasar Prasetya, Didik; Abadi, Moh Fairuz
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1673

Abstract

Islamic boarding schools are educational or non-formal educational institutions to learn more about religion. Boarding school is a place that has the potential to affect skin health. Skin infection is one of the diseases often suffered by students. One of them is a skin infection caused by fungi or better known as Tinea versicolor or tinea versicolor. Factors that can affect skin health include physical factors, chemical factors, and biological factors. This study aims to identify Malassezia spp fungi in the skin scrapings of students and determine the percentage or prevalence of Malassezia spp. fungi causing skin fungal infections in students at Hidayatullah Islamic Boarding School in Denpasar City. This type of research is descriptive with a population of all high school student council members, 17 male students and 23 female students with a total of 40 students. The results obtained from the examination were that there was 1 sample from male students who were positive for Malassezia spp. which causes Pityriasis versicolor or also called panu. Skin diseases can infect easily if you do not maintain personal hygiene.
UJI EFEKTIVITAS KOMBINASI PERASAN BUAH LABU AIR DAN JAHE MERAH SEBAGAI ANTIBAKTERI Salmonella typhi Djohan, Herlinda; Triana, Linda; Nurhayati, Etiek
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1584

Abstract

Typhoid fever is an acute disease caused by the bacteria Salmonella typhi. This bacteria is usually found in contaminated water or food. Apart from that, this bacteria can also be transmitted from infected people. The treatment carried out is using modern drugs such as the antibiotic chloramphenicol. However, the use of modern medicine has several disadvantages such as side effects. On the other hand, the use of natural ingredients as natural medicine has been widely used. One of them is that water gourd is empirically used as a fever reducer. Water gourd contains secondary metabolites, namely saponins, steroids and phenols which have been proven to have antibacterial activity and are able to inhibit the growth of Salmonella typhi bacteria. Another natural ingredient that has antibacterial activity is red ginger. Red ginger contains active compounds that have antibacterial effects such as flavonoids, phenols, treponoids and essential oils. Antibacterial activity was obtained by measuring the diameter of the inhibition zone after administering 4 variations of the concentration of the juice sample. The positive control used amoxicillin 30 µg/disk and the negative control used distilled water. The research design used was a quasi-experimental method. The samples were water gourd and red ginger juice with varying concentrations of 40%, 60%, 80% and 100% and the average inhibition zone values were obtained, namely 8.875 mm, 9.875 mm, 11.25 mm and 14.25 mm. Based on the results of research and computerized data processing using the Kruskall Walis test, a value of p = 0.000 (p<0.05) was obtained, which means that there is an effect of the concentration of pumpkin juice and red ginger on the inhibitory power of Salmonella thypi bacteria.
ANALISIS ANTI BAKTERI SEDIAAN GEL PEMBERSIH TANGAN EKSTRAK ETANOL DAUN BELIMBING WULUH TERHADAP BAKTERI Escherichia coli Sya’baniar, Luthfiranda; Suwandi, Edy; Ihsan, Bagus Muhammad; Sari, Emilda; Fatayati, Imma
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1492

Abstract

Antiseptik digunakan untuk membunuh atau menghambat pertumbuhan mikroorganisme pada jaringan hidup. Daun belimbing wuluh mengandung senyawa metabolit antibakteri seperti alkaloid, flavonoid, saponin, tanin, fenol, steroid dan triterpenoid. Tujuan penelitian ini menjelaskan perbedaan gel pembersih tangan ekstrak daun belimbing wuluh dua macam formula dalam menghambat Escherichia coli. Dengan purposive sampling, sampel yang digunakan adalah ekstrak daun belimbing wuluh dengan 2 perlakuan yaitu kosentrasi 7,5% dan 15% yang masing-masing dilakukan pengulangan sebanyak 16 kali dengan metode difusi cakram. Hasil penelitian yaitu sediaan gel pembersih tangan memiliki aktivitas antibakteri terhadap Escherichia coli pada formula I memiliki zona hambat rata-rata 7,4mm dan formula II memiliki zona hambat rata-rata 9,6mm kategori sedang. Hasil organoleptis warna hijau bau khas berbentuk semi solid dan rata-rata pH 6. Hasil uji Wilcoxon didapatkan p value 0,000 < α 0,05 artinya terdapat perbedaan gel pembersih tangan ekstrak daun belimbing wuluh formula I dan formula II dalam mengahambat pertumbuhan Escherichia coli.
Analisis Tekanan Darah pada Penderita Diabetes Melitus di Puskesmas Jungkat, Kalimantan Barat Nurhayati, Etiek; Yustiana Putri, Naimatul Jannah; Slamet, Slamet
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1682

Abstract

Diabetes mellitus is a chronic disease, the pancreas does not produce enough insulin, or when the body cannot use insulin effectively. Diabetes mellitus can be followed by various complications, including increased blood pressure or hypertension. Diabetes mellitus is one of the diseases with the 5th highest number of patients at the Jungkat Community Health Center. This study aims to analyze blood pressure in diabetes mellitus patients at the Jungkat Community Health Center, Mempawah Regency, West Kalimantan. This research method is a cross-sectional study. The respondents in this study were diabetes mellitus patients who received treatment at the Jungkat Community Health Center, using an incidental sampling technique.  Research on 40 respondents showed the results: the average systolic blood pressure was 158.03 mmHg, and the average diastolic blood pressure was 87.92 mmHg, while the average blood glucose level was 289.83 mg/dl. The results of statistical analysis using the Chi Square correlation test showed that the p-value (0.001) was smaller than α (0.05). The results of statistical analysis show that there is a relationship between blood pressure and blood glucose levels in diabetes mellitus patients at the Jungkat Community Health Center, Mempawah Regency, West Kalimantan.
Pemanfaatan Tithonia diversifolia Sebagai Bahan Alami Sabun Cair Terhadap Bakteri Staphylococcus aureus Metode Difusi Putra, Rengga Khatulistiwa; Suwandi, Edy; Sugito, Sugito
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1494

Abstract

Sabun adalah salah satu produk pembersih kulit yang paling banyak dan sudah sejak lama berabad-abad silam yang lalu telah digunakan yang didasarkan pada surfaktan. Sabun berbahan baku bahan alam masih jarang ditemukan di pasaran. Kebanyakan dari sabun tersebut masih menggunakan bahan sintetik sebagai bahan aktifnya. Daun insulin memiliki kandungan  senyawa antibakteri diantaranya seperti tanin, flavonoid, alkaloid, hingga saponin yang dapat dijadikan sebagai bahan aktif. Tujuan dari penelitian ini adalah untuk menjelaskan perbedaan sabun cair ekstrak daun insulin formula I dan formula II dalam menghambat pertumbuhan bakteri Staphylococcus aureus metode difusi. Penelitian ini berbentuk Quasi Experimental Design dengan teknik sampling purposive. Sampel yang digunakan adalah sediaan sabun cair esktrak daun insulin yang dibuat sebanyak 2 perlakuan, yaitu sediaan sabun cair ekstrak daun insulin formula I (ekstrak 10%) dan formula II (ekstrak 15%) yang dibuat 16 kali pengulangan pada masing-masing perlakuan, sehingga total sampel yang digunakan untuk 2 kelompok perlakuan adalah 32 sediaan yang diuji kekuatan antibakterinya dalam menghambat Staphylococcus aureus dengan mengunakan metode difusi cakram Kirby-Bauer. Hasil penelitian sediaan sabun cair ekstrak daun insulin didapatkan pengukuran zona hambat pada formula I rata-rata sebesar 9,5 mm kategori sedang dan formula II rata-rata sebesar 14,9 mm kategori kuat. Hasil analisis statistik uji Wilcoxon didapatkan p value 0,000 < α 0,05 yang berarti terdapat perbedaan diameter zona hambat pada sabun cair ekstrak daun insulin formula I dan II dalam menghambat pertumbuhan bakteri Staphylococcus aureus metode difusi.
Analisis Antibakteri Sediaan Sabun Cair Ekstrak Etanol Batang Bajakah (Spatholobus littoralis Hassk) Terhadap Bakteri Staphylococcus aureus Secara In Vitro Aprisca, Meisya; Sungkawa, Hendra Budi; Wahdaniah, Wahdaniah
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1542

Abstract

Bajakah adalah salah satu keragaman hayati yang tumbuh liar di tengah hutan, batang bajakah diketahui memiliki lebih dari satu kandungan senyawa kimia yang memiliki aktivitas farmakologi yang baik. Berdasarkan hasil pengujian fitokimia yang dilakukan pada ekstrak batang bajakah menunjukkan bahwa tumbuhan ini memiliki kandungan metabolit sekunder seperti alkaloid, flavonoid, tanin, saponin dan terpenoid yang dapat menghambat pertumbuhan bakteri sehingga dapat dimanfaatkan untuk menjadi alternative bahan pembuatan sabun cair. Tujuan dari penelitian ini adalah untuk menetapkan potensi aktivitas sediaan sabun cair ekstrak batang bajakah formula 1 (konsenstrasi 30%), formula 2 (konsentrasi 40%) dan formula 3 (konsentrasi 50%) terhadap diameter zona hambat pertumbuhan bakteri Staphylococcus aureus secara in vitro. Penelitian ini menggunakah metode difusi secara in vitro. Desain penelitian ini adalah (Quasi experiment). Populasi pada penelitian ini adalah sabun cair ekstrak etanol batang bajakah. Sampel pada penelitian ini adalah sabun cair ekstrak etanol batang bajakah formula 1 (konsenstrasi 30%), formula 2 (konsentrasi 40%) dan formula 3 (konsentrasi 50%) dengan 9 kali replikasi sehingga jumlah sampel yang digunakan adalah 27 sampel yang diuji kekuatannya terhadap Staphylococcus aureus. Hasil penelitian menunjukkan pada formula 1 (konsentrasi 30%) didapatkan diameter zona hambat sebesar 14,44 mm, pada formula 2 (konsentrasi 40%) didapatkan diameter zona hambat sebesar 15,22 mm, pada formula 3 (konsentrasi 50%) didapatkan diameter zona hambat sebesar 16,22 mm. Berdasarkan hasil penelitian disimpulkan bahwa pada formula 1 (konsentrasi 30%), formula 2 (konsentrasi 40%), dan formula 3 (konsentrasi 50%) adalah berpotensi kuat maka dapat disimpulkan bahwa hipotesis (H1) diterima.
POTENSI TEPUNG KULIT PISANG RAJA SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN JAMUR Penicillium sp. Kamilla, Laila; Fadilla, Alda Nur; Nuswantoro, Ari; Tumpuk, Sri
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1665

Abstract

Sumber karbohidrat yang mudah ditemukan dan belum banyak dimanfaatkan adalah tepung kulit pisang raja. Kulit pisang raja memiliki potensi untuk digunakan sebagai media alternatif karena memiliki kandungan zat seperti karbohidrat, protein, dan lemak. Tujuan penelitian ini untuk mengetahui potensi tepung kulit pisang raja sebagai media pertumbuhan jamur Penicillium sp. Penelitian ini menggunakan desain penelitian Quasi Experiment. Populasi dalam penelitian ini yaitu kulit pisang raja. Sampel yang digunakan adalah tepung kulit pisang raja yang dibuat dalam konsentrasi 10%, 20%, 30%, 40%, 50% dengan 5 kali pengulangan didapatkan jumlah sampel 25. Hasil penelitian yaitu konsentrasi 10% rata- rata 11,6 CFU, konsentrasi 20% rata-rata 12,8 CFU, konsentrasi 30% rata-rata 17,4 CFU, konsentrasi 40% rata-rata 21,8 CFU, konsentrasi 50% rata-rata 28 CFU dan media standar Sabouraud Dextrose Agar (SDA) koloni diperoleh rata-rata 152,6 CFU. Hasil uji Anova didapatkan nilai 0,000 < 0,05 yang menyatakan ada perbedaan pertumbuhan jumlah koloni pada tiap konsentrasi media alternatif dengan media standar Sabouraud Dextrose Agar (SDA) dapat disimpulkan media alternatif tepung kulit pisang raja berpotensi digunakan sebagai media pertumbuhan jamur Penicillium sp. namun tidak dapat menggantikan media standar Sabouraud Dextrose Agar (SDA).
Evaluasi Robustness Metode Deteksi Cepat Berbasis MPN untuk Legionella pneumophila terhadap Gangguan Mikroflora Non-Target pada Sampel Air dari Menara Pendingin Ulfia, Phisa Isyah; Sungkawa, Hendra Budi; Fatayati, Imma
Jurnal Laboratorium Khatulistiwa Vol 8, No 2 (2025): Mei 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i2.1887

Abstract

Legionellosis is an infection caused by Legionella pneumophila, commonly transmitted through artificial water systems such as cooling towers. Early detection of this pathogen is crucial, particularly in high-risk facilities. This study aimed to assess the robustness of a rapid detection method based on the Most Probable Number (MPN) principle, focusing on the effectiveness of pretreatment in reducing interference from non-target microorganisms. A total of 30 positive trays from Legiolert™ tests were subcultured on Tryptic Soy Agar (TSA) to identify any surviving non-target microbes. Robustness was defined by the proportion of trays without non-target microbial growth. Results showed a robustness rate of 93.33%, with full robustness (100%) observed in low and moderate microbial load groups, and 75% in the high-load group. A non-parametric binomial test using SPSS was performed to evaluate the statistical significance of the deviation from the robustness cut-off. The resulting p-value was 0.446 (p > 0.05), indicating no statistically significant difference. These findings demonstrate that the Legiolert™ method maintains reliable performance even in complex sample matrices, supporting its use in routine water quality monitoring in accordance with Indonesian Ministry of Health Regulation No. 2 of 2023.

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