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INDONESIA
AL KAUNIYAH
Published by Universitas Islam Negeri Syarif Hidayatullah Jakarta
ISSN : 19783736     EISSN : 25026720     DOI : 10.15408/kauniyah
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Materials Science & Nanotechnology
Al-Kauniyah: Jurnal Biologi (p-ISSN: 1978-3736, e-ISSN: 2502-6720) is an Open Access Journal published by Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islamic University Jakarta, and established since 2007. Since 2016 Al-Kauniyah has established a collaboration with the Association of Lecturer in Biology and Biology Education throughout the State Islamic Higher University (PTKIN) in Indonesia. Until 2015, Al-Kauniyah covered environmental biology solely, but since 2016 the journal has been extended to cover the entire field of biological science (bioscience). By publishing biannually, on April and October, Al-Kauniyah is intended to communicate original researches and current issues on the subject of biology. Since volume 9 issue 1 April 2016, Al-Kauniyah had been changes the layout. This journal warmly welcomes contributions from scholars of related disciplines. Manuscripts can be submitted to AL-KAUNIYAH
Arjuna Subject : -
Articles 460 Documents
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The Effect of Moringa Leaf Extract Administration on Sperm Morphology and Blood Glucose Reduction in Alloxan-Induced Sprague Dawley Rats Wahab, Dinda Nuraini Hanifah; Hasanah, Uswatun; Harfiani, Erna; Thadeus, Maria Selvester
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.37286

Abstract

AbstractIncreased blood sugar levels can be triggered by increased intake or insulin resistance, leading to increased oxidative stress that affects sperm quality during spermatogenesis. Moringa oleifera, rich in antioxidants, has been proven effective in improving sperm quality through several studies. This study aims to evaluate the influence of moringa leaf extract on sperm morphology and the reduction of blood sugar levels in Sprague Dawley white rats (Rattus norvegicus) induced by alloxan. Fasting blood sugar tests showed that rats induced with alloxan without moringa leaf extract had the highest blood sugar levels among the groups. In contrast, the negative control and treatment groups with moringa leaf extract successfully maintained blood sugar levels at normal levels. Normal sperm morphology reached 94.5% in the treatment group with the highest dose of moringa leaf extract. The results of the study indicate a significant relationship between blood sugar levels and sperm morphology in alloxan-induced white rats after the administration of moringa leaf extract. Moringa oleifera has the potential to be a therapeutic intervention to improve sperm quality and control blood sugar levels in hyperglycemic conditions.AbstrakPeningkatan kadar gula darah dapat dipicu oleh peningkatan asupan atau resistensi insulin menyebabkan peningkatan stres oksidatif yang mempengaruhi kualitas sperma selama spermatogenesis. Tanaman kelor (Moringa oleifera), tumbuhan yang kaya akan antioksidan, telah terbukti efektif dalam meningkatkan kualitas sperma melalui beberapa penelitian. Penelitian ini bertujuan untuk mengevaluasi pengaruh ekstrak daun kelor terhadap morfologi sperma dan pengurangan kadar gula darah pada tikus putih Sprague Dawley (Rattus norvegicus) yang diinduksi aloksan. Uji gula darah puasa menunjukkan bahwa tikus yang diinduksi aloksan tanpa perlakuan ekstrak daun kelor memiliki kadar gula darah tertinggi di antara kelompok-kelompok lainnya. Sebaliknya, kelompok kontrol negatif dan kelompok perlakuan dengan ekstrak daun kelor berhasil menjaga kadar gula darah pada tingkat normal. Morfologi sperma normal mencapai 94,5% pada kelompok perlakuan dengan dosis 400 mg/kgBB ekstrak daun kelor. Kesimpulan yang didapatkan pada penelitian ini berkaitan dengan temuan hubungan signifikan antara kadar gula darah dan morfologi sperma pada tikus putih yang diinduksi aloksan setelah pemberian ekstrak daun kelor. Tanaman kelor (Moringa oleifera) memiliki potensi sebagai intervensi terapeutik untuk meningkatkan kualitas sperma dan mengendalikan kadar gula darah dalam kondisi hiperglikemia. 
Antifungal, Hydrolytic Enzyme Activity, and Identification of Gut Bacterial in Feces of Black Soldier Fly (Hermetia illucens) Larvae Maulida, Meirina Nur; Fatmawati, Umi
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.37354

Abstract

AbstractBlack soldier fly (BSF) (Hermetia illucens) is a type of fly that has larvae called maggots with potential as a waste bioremediation agent. Maggot from BSF has unique digestive characteristics with a diversity of bacteria in it which helps maggot digest organic materials by producing various hydrolytic enzymes. Fecal bacteria in maggots also have potential antifungal activity. This research aimed to identify the hydrolytic and antifungal enzyme activity of maggot fecal bacteria (Hermetia illucencs). Hydrolytic enzyme activity is carried out by measuring the hydrolytic zone in the test medium. The antifungal antagonist test was carried out on Phytophthora sp. using the dual agar culture method. The results of the enzyme activity test showed that isolate MNM 001 had proteolytic enzyme activity, MNM 002 had amylolytic, and proteolytic enzyme activity, and was able to dissolve P elements. MNM 003 had cellulolytic, amylolytic, and proteolytic enzyme activity. From the results of the antagonist test, MNM 001, MNM 002, and MNM 003 have antifungal activity against Phytophthora sp. of the three isolates, isolates MNM 002 and MNM 003 had the best hydrolytic enzyme activity and were identified using the 16S rRNA gene. The results of amplification of the 16S rRNA gene from MNM 002 and MNM 003 indicated that the two isolates were close to the genus Brevibacterium.AbstrakBlack soldier fly (BSF) (Hermetia illucens) adalah jenis lalat yang memiliki larva disebut maggot dengan potensi sebagai agen bioremediasi sampah. Maggot dari BSF memiliki karakterisitik pencernaan unik dengan keragaman bakteri di dalamnya yang membantu maggot mencerna bahan organik dengan menghasilkan beragam enzim hidrolitik. Bakteri pada feses maggot juga memiliki potensi aktivitas antifungi. Tujuan dari penelitian ini adalah untuk mengidentifikasi aktivitas enzim hidrolitik dan antifungi dari bakteri feses maggot (Hermetia illucens). Aktivitas enzim hidrolitik dilakukan dengan mengukur zona hidrolitik pada medium uji. Uji antagonis antifungi dilakukan terhadap Phytophthora sp. dengan metode dual culture agar. Hasil uji aktivitas enzyme menunjukkan isolat MNM 001 memiliki aktivitas enzim proteolitik, MNM 002 memiliki aktivitas enzim amilolitik, proteolitik, dan mampu melarutkan unsur P. MNM 003 memiliki aktivitas enzim selulolitik, amilolitik, dan proteolitik. Hasil uji antagonis MNM 001, MNM 002, dan MNM 003 memiliki aktivitas antifungi terhadap Phytophthora sp. Ketiga isolat tersebut, isolate MNM 001, MNM 002, dan MNM 003 memiliki aktivitas enzim hidrolitik terbaik dan diidentifikasi menggunakan gen 16S rRNA. Hasil amplifikasi gen 16S rRNA dari MNM 002 dan MNM 003 mengindikasikan bahwa kedua isolat tersebut memiliki kedekatan dengan genus Brevibacterium.
Effect of EM4 (Effective Microorganism 4) on Growth and Productivity of Cucumber (Cucumis sativus L.) Yuniati, Ratna; Damayanti, Margaretta Elsa; Wardhana, Wisnu
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.37561

Abstract

AbstractCucumber (Cucumis sativus L.) is a nutritious and healthy vegetable that is commonly consumed by Indonesian people. To fulfill self-sufficiency for household scale needs during the COVID-19 pandemic, cucumber cultivation can be carried out in home gardens, using containers such as polybags. Growing cucumbers on limited land requires a carefully optimized planting media composition by applying Effective Microorganism 4 (EM4) to the polybag media when planting. The research has been conducted which aims to determine the best EM4 dosage for the growth and productivity of cucumbers. The study used a Randomized Block Design consisting of control and three treatment doses of 10% concentration EM4, namely 20, 40, and 60 mL per polybag with six replications. The planting media used is a mixture of loam soil and goat manure. NPK fertilizer is given as an additional nutrient. The EM4 application is done by pouring it every eight days into the planting media in polybags. The results showed an increase in growth parameters and productivity of cucumber plants namely plant height, leaf chlorophyll content, time of flower emergence, number of flowers, and number of flowers that form fruit. 40 mL EM4 is the dose that showed the highest growth and productivity.AbstrakMentimun (Cucumis sativus L.) merupakan sayuran bergizi dan menyehatkan yang banyak dikonsumsi masyarakat Indonesia. Untuk memenuhi swasembada kebutuhan skala rumah tangga di masa pandemi COVID-19, budidaya mentimun dapat dilakukan di pekarangan rumah, dengan menggunakan wadah polybag. Menanam mentimun di lahan terbatas memerlukan optimalisasi komposisi media tanam secara cermat dengan menerapkan Effective Microorganism 4 (EM4) pada media dalam polybag. Telah dilakukan penelitian yang bertujuan untuk mengetahui dosis EM4 terbaik untuk pertumbuhan dan produktivitas tanaman mentimun. Penelitian menggunakan Rancangan Acak Kelompok yang terdiri atas kontrol dan tiga perlakuan dosis EM4 konsentrasi 10% yaitu 20, 40, dan 60 mL per polybag dengan enam ulangan. Media tanam yang digunakan adalah campuran tanah lempung dan kotoran kambing. Pupuk NPK diberikan sebagai unsur hara tambahan. Penerapan EM4 dilakukan dengan cara disiram setiap delapan hari sekali ke dalam media tanam di polybag. Hasil penelitian menunjukkan adanya peningkatan parameter pertumbuhan dan produktivitas tanaman mentimun yaitu tinggi tanaman, kandungan klorofil daun, waktu munculnya bunga, jumlah bunga, dan jumlah bunga yang membentuk buah. Dosis yang menunjukkan pertumbuhan dan produktivitas tertinggi adalah 40 mL EM4.
Analysis of Turtle Conservation Activities Effectiveness on Kelapa Dua Island, Kepulauan Seribu Gulo, Graciella Stevani; Tumuyu, Sri Setiawati; Patria, Mufti Petala
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.37694

Abstract

AbstractThe exploitation of turtles has resulted in a decline in the turtle population. The relocation of turtle eggs from nesting habitats is a widely accepted conservation practice. This research aims to analyze the effectiveness of turtle conservation activities on Kelapa Dua Island. The study adopts a mixed-methods approach, collecting primary data through field observations and interviews and secondary data from the Kepulauan Seribu National Park Office (BTNKpS). The collected data includes information on turtle nest monitoring activities, turtle preservation techniques, and the hatching success rate. The research results show that the hawksbill turtle (Eretmochelys imbricata) is the most commonly found turtle species. The average hatching success rate over the past six years is 71.98%. This value can still be optimized to reach 80% by establishing hatcheries on the nesting islands or islands near the nesting sites. Through this strategy, monitoring can be conducted more regularly, the turtle egg relocation process can be carried out relatively quickly, and vibrations or shocks to the turtle eggs during transportation can be minimized, thus increasing the hatching success rate. Regular monitoring of the environmental conditions of the artificial nests, including temperature, pH, and humidity, is also essential to improve the hatching percentage.AbstrakEksploitasi penyu telah menyebabkan penurunan populasi penyu. Relokasi telur penyu dari habitat penetasan adalah praktik konservasi yang umum diterima. Penelitian ini bertujuan untuk menganalisis efektivitas kegiatan konservasi penyu di Pulau Kelapa Dua. Studi ini mengadopsi pendekatan metode campuran, mengumpulkan data primer melalui observasi lapangan dan wawancara serta data sekunder dari Balai Taman Nasional Kepulauan Seribu (BTNKpS). Data yang terkumpul meliputi informasi tentang kegiatan pemantauan sarang penyu, teknik pelestarian penyu, dan tingkat keberhasilan penetasan. Hasil penelitian menunjukkan bahwa penyu sisik (Eretmochelys imbricata) merupakan spesies penyu yang paling banyak ditemukan. Tingkat keberhasilan penetasan rata-rata selama enam tahun terakhir adalah 71,98%. Nilai ini masih bisa dioptimalkan hingga mencapai 80% dengan mendirikan hatchery di pulau-pulau peneluran atau pulau-pulau yang berdekatan dengan lokasi peneluran. Melalui strategi ini, pemantauan dapat dilakukan lebih rutin, proses relokasi telur penyu dapat dilakukan dengan relatif cepat, dan getaran atau benturan pada telur penyu selama proses transportasi dapat diminimalkan, sehingga meningkatkan tingkat keberhasilan penetasan. Pemantauan teratur terhadap kondisi lingkungan sarang buatan, termasuk suhu, pH, dan kelembaban, juga penting untuk meningkatkan persentase penetasan. 
Endophytic Bacteria From Banana Plants Improves The Growth of Rice (Oryza sativa) and Maize Plants (Zea mays) Sidiq, Yasir; Silviani, Defina Anggita; Rahayu, Triastuti; Saputra, Alanindra; Wahibah, Ninik Nihayatul; Widianto, Donny
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.37884

Abstract

AbstractFive isolates of endophytic bacteria from the roots of klutuk and ambon banana plants produce indole acetic acid (IAA) hormone. However, the evidence of its potential as Plant Growth Promoting Bacteria (PGPB) has not been observed. Therefore, this study aims to determine the effect of bacterial isolate to increase the growth of rice and maize plants. The growth rates of bacterial isolates were measured before the inoculation into the plant root. The root inoculation was performed following a complete randomized design. Root inoculation was carried out using 5 isolates of endophytic bacteria in rice and maize plant seedlings, and the process was repeated 15 times (n=15). The growth of rice and maize plants was monitored from 1st day after planting (dap) to the 14th dap. The collected data were analyzed by student t-test with a significant level of 5% (a= 0.05). The results showed that the logarithmic phase of all isolates was reached at 4 to 16 hours and K10, K25, K35, K111, and A41 isolates increased the height of plants while the K111 isolate can significantly increase the plant height compared to the control. This experiment revealed that the endophytic bacteria from banana plants increase the rice and maize plant’s growth.AbstrakLima isolat bakteri menghasilkan hormon indole acetic acid (IAA) yang berpotensi memacu pertumbuhan tanaman. Namun, bukti empiris potensinya sebagai Plant Growth Promoter Bacteria (PGPB) belum didapatkan. Penelitian ini bertujuan untuk mengetahui pengaruh isolat bakteri terhadap peningkatan pertumbuhan padi dan jagung. Penelitian ini menggunakan metode eksperimen dengan desain rancangan acak lengkap dan setiap perlakuan diulang 15 kali (n= 15). Hasil pertumbuhan tanaman yang diinokulasi dengan isolat bakteri masing-masing dibandingkan dengan perlakuan kontrol. Sebelum perlakuan, tingkat pertumbuhan bakteri endofit diukur sehingga didapatkan fase logaritmik yang cocok untuk dilakukan inokulasi ke akar tanaman. Setelah perlakuan, pertumbuhan tanaman padi dan jagung dipantau dari hari ke-1 setelah tanam (hat) sampai dengan ke-14 hat. Data dianalisis dengan uji t dengan taraf signifikan 5% (α= 0,05). Hasil penelitian menunjukkan bahwa 5 isolat yang berasal dari tanaman pisang mengalami fase logaritmik pada masa inkubasi 4 hingga 16 jam dan semua isolat bakteri meningkatkan tinggi tanaman serta isolat K111 dapat meningkatkan tinggi tanaman secara signifikan dibandingkan dengan kontrol. Penelitian ini mengungkapkan kemampuan bakteri endofit dari tanaman pisang untuk meningkatkan pertumbuhan tanaman.
Antibacterial Activity of Bacterial Pigment Extracts Isolated From Fruit and Vegetable Waste Against Staphylococcus epidermidis Salsabila, Haniya; Maharani, Andrea; Siregar, Abdul Rahman
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.37936

Abstract

Acne affects approximately 9.4% of the global population and become one of the big eight skin diseases due to Staphylococcus epidermidis infection. This infection can be treated using bacterial pigments for their potential activities as antioxidant, anticancer, and antimicrobial with low toxicity and stable productivity. In this study, pigments were harvested and purified from pigment-producing bacteria which were isolated from fruit and vegetable waste, and the antibacterial activity was conducted with disc diffusion method against S. epidermidis. There were three pigment-producing isolates (LBS 6, LBS 12, and LBS 14) that produced green pigments with antibacterial activity against S. epidermidis. Among the pigments produced by the three isolates, pigments from LBS 14 had the widest zone of inhibition and the strongest antibacterial activity followed by LBS 6 and LBS 12 respectively. In addition, through Two-Way ANOVA analysis, it was found that there was a significant effect on the utilization of pigments from different bacterial isolates and variations in concentration on the diameter of the inhibition zone as well as the interaction between them
Bacteriocin Activity of Lactic Acid Bacteria from Giant Prawn (Macrobrachium rosenbergii) Meysari, Della; Helmi, Henny; Lingga, Rahmad
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.38124

Abstract

AbstractFood is one of the necessities of life. Food is often added with preservatives such as chemicals that harm human health. One of the safe natural preservatives is bacteriocin compounds. Bacteriocins can be produced by lactic acid bacteria (LAB). These bacteriocins have known as Generally Recognized as Safe (GRAS) status. This study aimed to isolate and identify BAL from the digestive tract of giant shrimp (Macrobrachium rosenbergii), as well as test the ability of the bacteriocin produced to the proteolytic enzyme, temperature, pH, and salt. The research methods used were bacterial isolation, bacterial characterization, hemolysis test, bacteriocin antibacterial activity tests, proteolytic enzyme influence tests on bacteriocin activity, temperature, pH, and salt content tests on bacteriocin activity, and antibiotic tests. The research results showed that there were 37 LAB isolates and there were 7 isolates that produced bacteriocins. The LAB isolated from the digestive tract of giant prawns is Gram-positive bacteria in the form of bacilli, catalase-negative, gamma hemolytic, methyl red positive, and homofermentative. The bacteriocins can inhibit the pathogenic bacteria Staphylococcus aureus and Escherichia coli and be degraded by the Protease-K enzyme. Moreover, the bacteriocins have the characteristics of being stable at acid to neutral pH (pH 2–7), stable at low and high temperatures (4–100 °C), and stable under conditions with a salt content of 2–6.5%. The results of the identification of LAB belonged to the Lactobacillus genus.AbstrakMakanan merupakan kebutuhan pokok dalam kehidupan sehari-hari manusia. Makanan sering kali ditambahkan bahan pengawet seperti bahan kimia yang berpengaruh buruk terhadap kesehatan manusia. Salah satu alternatif bahan pengawet alami yang aman bagi kesehatan manusia adalah senyawa bakteriosin. Bakteriosin dapat dihasilkan dari bakteri asam laktat (BAL). Bakteriosin yang diproduksi oleh BAL sudah berstatus Generally Recognized as Safe (GRAS). Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi BAL dari saluran pencernaan udang galah (Macrobrachium rosenbergii), serta menguji kemampuan bakteriosin yang dihasilkan terhadap enzim proteolitik, suhu, pH dan kadar garam. Metode penelitian yang dilakukan adalah isolasi bakteri, karakterisasi bakteri, uji hemolisis, uji aktivitas antibakteri bakteriosin, uji pengaruh enzim proteolitik, suhu, pH dan kadar garam terhadap aktivitas bakteriosin. Hasil isolasi terdapat 37 isolat BAL dan 7 isolat yang menghasilkan bakteriosin. BAL yang diisolasi dari saluran pencernaan udang galah merupakan bakteri Gram positif berbentuk basil, katalase negatif, gamma hemolisis, methyl red positif dan homofermentatif. Bakteriosin mampu menghambat bakteri patogen Staphylococcus aureus dan Escherichia coli, dapat didegradasi oleh enzim Protease-K, stabil pada pH asam hingga netral (pH 2–7), stabil pada suhu rendah maupun tinggi (4°–100 °C) dan stabil pada kondisi dengan kadar garam 2–6,5%.  Hasil identifikasi BAL dari usus udang galah yaitu bakteri termasuk dalam Genus Lactobacillus.
Isolation And Characterization of Bacteria from Shallots (Allium cepa L.) as In-vitro Biocontrol Agent of Fusarium oxysporum f.sp cepae Radiastuti, Nani; Amalia, Wuliani; Suryadi, Yadi; Susilowati, Dwi N; Kosasih, Jajang
Al-Kauniyah: Jurnal Biologi Vol. 17 No. 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i2.38132

Abstract

AbstractShallot is one of the leading vegetable commodities with many benefits such as for seasonings and herbal medicinal ingredients. The demand for shallots continues to increase; however, shallot production is still relatively low. One of the limiting factors causing low shallot production is due to wilt disease caused by Fusarium oxysporum f.sp cepae (Foc). Bacteria have many roles in suppressing the growth of Foc, and this study aims to obtain potential bacterial isolates from the shallot plant to inhibit the growth of Foc Based on fungal diameter zone inhibition, degree of inhibition, and chitinase test, it was obtained 9 isolates which could suppress the growth of Foc. The results indicated that the AB3, TB2, and UB1 bacterial isolates could inhibit the growth of Foc with a percentage of inhibition of 46.80; 40.24; and 35.11%, respectively. The analysis showed that AB3, TB2, and UB1 isolates were categorized as moderate in suppressing the growth of Foc. The 16S rRNA sequencing results showed that AB3 and TB2 isolate had similarities with Bacillus subtilis by 99,75%, and 100%, respectively, while UB1 isolate had similarities with Pseudomonas nitroreducens by 89,35%. Based on the result showed that Bacillus sp. AB3 and TB2 isolates, and P. nitroreducens UB1 isolate have more potential as biological control agents to control the Fusarium wilt at in vitro assay. The field efficacy studies on these potential antagonists need to be done in the future.AbstrakSalah satu faktor pembatas yang menyebabkan rendahnya produksi bawang merah adalah penyakit layu yang disebabkan oleh Fusarium oxysporum f.sp cepae (Foc). Bakteri antagonis memiliki banyak peran dalam menekan pertumbuhan Foc, dan penelitian ini bertujuan untuk mendapatkan isolat bakteri antagonis potensial asal tanaman bawang merah untuk menghambat pertumbuhan Foc. Berdasarkan nilai zona hambat diameter jamur, derajat hambat dan uji kitinase, diperoleh 9 isolat yang dapat menekan pertumbuhan Foc. Hasil penelitian menunjukkan bahwa isolat bakteri AB3, TB2, dan UB1 mampu menghambat pertumbuhan Foc dengan persentase penghambatan masing-masing sebesar 46,80; 40,24; dan 35,11% dengan kategori penghambatan pertumbuhan Foc moderat. Hasil sekuensing 16S rRNA, menunjukkan bahwa isolat AB3 dan TB2 memiliki kemiripan dengan Bacillus subtilis masing-masing sebesar 99,75%, dan 100%, sedangkan isolat UB1 memiliki kemiripan dengan Pseudomonas nitroreducens sebesar 89,35%. Berdasarkan hasil penelitian menunjukkan bahwa Bacillus sp. isolat AB3, TB2, dan P.nitroreducens isolat UB1 berpotensi digunakan sebagai agen pengendali hayati untuk mengendalikan penyakit layu Fusarium pada uji in vitro. Studi kemanjuran lapangan terhadap isolat antagonis potensial ini perlu dilakukan di masa depan.
Analysis of Microglia Morphology and Number in Wistar Rats Brain After CIDR1α-PfEMP1 Recombinant Protein Injection Sulistyaningsih, Erma; Putri, Izza Amalia; Rachmania, Sheilla
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.38242

Abstract

AbstractOne malaria vaccine candidate is Cysteine-rich Interdomain Region 1α (CIDR1α) of Plasmodium falciparumErythrocyte Membrane Protein 1 (PfEMP1), an essential protein involved in the pathogenesis of cerebral malaria. Microglia in the brain act as the first line of defense against brain pathological changes. The study aimed to evaluate the response of brain microglia to the CIDR1α-PfEMP1 recombinant protein injection by observing microglia morphology and number in rat’s cerebral cortex. 12 Wistar rats were divided into the control group, which was injected with normal saline solution, and the treatment group, which was injected with 150 µg CIDR1α-PfEMP1 recombinant protein combined with adjuvants. Injection was conducted thrice within three-week intervals (day 1, 21, and 42). Wistar rats were euthanized on day 56, and histological slides were prepared with Hematoxylin-Eosin staining. Examination using a microscope, 400x, and Fiji Image J software showed microglia morphology of ramified and rod cells in both the control and treatment groups. The microglia number in the control group was 93.00 ± 5.77, and the treatment group was 105.75 ± 15.62. Statistical analysis using an independent t-test showed no significant differences between groups (p= 0.15). The result indicated that the injection of CIDR1α-PfEMP1 recombinant protein did not provoke pathological changes in brain tissue, which induced a microglia response. This study strengthens the potential of the CIDR1α-PfEMP1 recombinant protein as a peptide-based malaria vaccine candidate.AbstrakSalah satu kandidat vaksin malaria adalah Cysteine-rich Interdomain Region 1α (CIDR1α) dari Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1), protein penting dalam patogenesis malaria serebral. Mikroglia di otak berperan sebagai pertahanan lini pertama terhadap perubahan di otak. Penelitian ini bertujuan mengevaluasi respon mikroglia otak terhadap pemberian protein rekombinan CIDR1α-PfEMP1 dengan mengamati morfologi dan jumlah mikrolia pada korteks serebri otak tikus. 12 tikus Wistar dibagi dalam kelompok kontrol yang diinjeksi normal saline dan  kelompok perlakuan diinjeksi 150 µg protein rekombinan CIDR1α-PfEMP1 yang dikombinasikan dengan adjuvant. Injeksi dilakukan tiga kali dengan interval tiga minggu (hari 1, 21, dan 42). Tikus dieuthanasia pada hari ke-56 dan preparat histologi otak disiapkan dengan pengecatan Hematoxyline-Eosin. Pengamatan menggunakan mikroskop 400x dan Fiji Image J software menunjukkan morfologi ramified dan rod cell pada kelompok kontrol maupun perlakuan. Jumlah mikroglia pada kelompok kontrol 93,00 ± 5,7 sedangkan kelompok perlakuan 105,75 ± 15,62). Analisis statistik menggunakan independent-t test menunjukkan tidak terdapat perbedaan yang bermakna antara 2 kelompok (p= 0,15). Hasil ini mengindikasikan bahwa pemberian protein rekombinan CIDR1α-PfEMP1 tidak menimbulkan patologi pada jaringan otak yang memicu respon mikroglia. Hal ini menguatkan potensi protein rekombinan CIDR1α-PfEMP1 sebagai kandidat vaksin malaria berbasis peptida.
Differences in Knowledge, Attitude, and Practice of The Community Parrots in Ternate and Sofifi, North Maluku Ramadani, Annisa; Setia, Tatang Mitra; Mangunjaya, Fachruddin M.; Siregar, Benny A.; Basalamah, Fitriah
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.38275

Abstract

 Abstract The community's habit of owning wild animals is still ongoing until now. This has become one of the threats to the existence of wildlife in their natural habitat. Parrots, with their ability to imitate surrounding sounds, have become a popular pet choice. Therefore, the purpose of this study is to determine the influence of knowledge, attitudes, and practices about parrots on the profile of the community. The study differentiates between those who owner bird and those who do not. Data collection was conducted for two months in Ternate and Sofifi, North Maluku. The method used was purposive sampling, interviewing as many as 104 respondents who had been determined. The interview data was processed into multiple linear regression values and canonical correlations. The results of this research identified that the majority of those who owned parrots were aged between 42–57 (36%); female (52%); with high school graduates (46%); and engaged in various professions, such as housewives or entrepreneurs (71%). On the other hand, those who were non-owner parrots were dominated by individuals aged 10–25 (46%); male (65%); with undergraduate degrees (62%); and categorized as students (38%). The results of this study show that knowledge and attitudes have an influence on education, profession, and age of the community on the topic of curly-billed bird maintenance. Abstrak Kebiasaan masyarakat memiliki satwa liar masih berlangsung hingga sekarang. Hal ini menjadi salah satu ancaman terhadap keberadaan satwa liar di habitat aslinya. Burung paruh bengkok memiliki kemampuan meniru suara di sekitarnya, dan menjadi satwa liar yang populer sebagai peliharaan. Oleh karena itu, tujuan penelitian ini adalah untuk mengetahui pengaruh pengetahuan, sikap, dan kesadaran tentang pemeliharaan burung paruh bengkok terhadap profil masyarakat. Studi ini membedakan, antara masyarakat yang memelihara burung dan yang tidak. Pengumpulan data dilakukan selama dua bulan di Kota Ternate dan Sofifi, Maluku Utara. Metode yang digunakan purposive sampling, mewawancarai sebanyak 104 responden yang telah ditentukan. Data wawancara tersebut diolah menggunakan software SPSS 22.0 untuk memperoleh nilai regresi linier berganda dan kolerasi kanonikal. Hasil penelitian, mengidentifikasi bahwa mayoritas dari mereka yang memiliki burung paruh bengkok berusia antara 42–57 (36%); berjenis kelamin perempuan (52%); tingkat pendidikan SMA (46%); dan memiliki profesi lainnya, seperti ibu rumah tangga atau pengusaha (71%). Di sisi lain, masyarakat yang tidak memelihara burung paruh bengkok, didominasi yang berusia 10–25 (46%); berjenis kelamin laki-laki (65%); tingkat pendidikan sarjana (62%); dan lebih ddidominasi oleh pelajar/mahasiswa (38%). Hasil penelitian ini menunjukkan bahwa pengetahuan dan sikap memiliki pengaruh dengan pendidikan, profesi, dan usia masyarakat terhadap topik pemeliharaan burung paruh bengkok.

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