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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Distribusi Gen Enterotoksin Staphylococcus aureus dari Susu Segar dan Pangan Asal Hewan Siti Isrina Oktavia Salasia; Khusnan -; Sugiyono -
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Staphylococcus aureus is a potential pathogen causing disease in human and animals due to severalvirulence factors. Staphylococcal enterotoxins which is responsible for foodborne disease is considered tobe one of the important virulence factor for the bacteria. The research was conducted to identify variousenterotoxin genes of S. aureus. Twenty three S. aureus isolates from milk cows (12 isolates) and foodanimal products (11 isolates) were used to study various enterotoxins genes (sea, seb, sec, sed, see, seg, seh,sei, and sej). The enterotoxins genes of S. aureus were investigated by using polymerase chain reaction(PCR) with specific primers. There were 3 isolates (13.04%) negative for staphylococcal enterotoxin genes.Twenty isolates (86.96%) harboured for one or more staphylococcal enterotoxin genes such as: sec (6 isolates/26.09%), see, seh for 1 isolate (4.35%), combination of 2 genes se (b,i), se (c,g), se (g,i) for 1 isolate of each(4.35%), se(c,e) for 2 isolates (8.70%), se(b,c) for 4 isolates (17.39%). Staphylococcal enterotoxin could bedetected in 3 combination genes of se(b,c,i), se(c,e,i), se(c,g,i) for 1 isolate of each (4.35%).
Re-Evaluate Interrelationship Dose-Response of Diminazene Aceturate Against to Infected Mice of Trypanosoma Evansi Bangkalan Isolates Muchamad Lazuardi; Muhamad Teguh Wahyudi; Ratna Sofaria Munier
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In vivo drug experiment involving Trypanosoma evansi (T.evansi) of Bangkalan isolated from outbreakof surra at 1988 in Madura Island, (Indonesia) infected mice  were performed. Single dose of diminazeneaceturate at the range of dose 5.0 to 9.7 mg/kg bw intra peritoneal were tested against 4th parasitaemicgrade level of infected mice. The assesment of trypanocide dose was using  probit test analysis at confidenceinterval  95% after observing trial groups data from thick blood smear examination of vein tail. Apparentlythe dose of diminazene at  6.858 mg/kg bwt had eliminated 50% of the bloodstream parasites and 9.138mg/kg bw for  99% had eliminated parasites. The research result indicates that Bangkalan isolate of T.evansi had expressed reduced susceptible to diminazene more than 1.3 times than regimentation dose toresearch report on 2005 year at the same research subject namely mice (at about 5.22 mg/kg bw).
Diagnosis and Molecular Marker Analysis of Bali’s Rabies Virus Isolates (DIAGNOSIS DAN ANALISIS PENANDA MOLEKULER VIRUS RABIES ISOLAT BALI) I Nyoman Dibia; Bambang Sumiarto; Heru Susetya; Anak Agung Gde Putra; I Gusti Ngurah Kade Mahardika; Helen Scott-Orr
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The direct fluorescent antibody test (dFAT) was recommended by both World Health Organization(WHO) and Office International des Epizooties (OIE) as a standard diagnostic technique for rabies. Sincethe outbreak of rabies in Bali, it was ascertain the importance to develop a reverse transcriptase-polymerasechain reaction (RT-PCR) technique with specific primers as an alternative diagnostic method. The aim ofthis study was to develop a RT-PCR technique for rabies diagnosis in animals and find out the molecularmarker of Bali’s rabies virus (BRV) isolates based on the sequence of nucleoprotein (N) gene. Brainsamples were obtained during 2009 from 14 suspected rabid dogs and one cattle, where rabies viruseswere isolated. The dFAT was used to detect the presence of rabies viral antigen. Ribonucleic acid (RNA) ofrabies viruses was extracted with TRIzol reagent. Fragment of N gene was amplified using one-step RTPCRmethod with specifically-designed primer pairs and sequenced using ABI automatic sequencer. Multiplealignment of nucleotide and deduced amino acid sequences were analyzed using ClustalW of MEGA 4.0program. This study found that twelve out of fifteen animal brain samples confirmed as rabies by dFAT.Similarly, a single band of 1215 bp PCR product for rabies virus was also detected in twelve out of twelve(100%) dFAT rabies positive samples. It is therefore evident that alternative diagnostic of rabies inanimals can be established using RT-PCR technique. The results showed that the RT-PCR has a very highagreement with dFAT. Polymorphic sites of N gene of twelve BRV isolates were identified at the position186, 501, 801, 840, 1068 and 1153. Bali’s rabies virus isolates have conserved amino acid (isoleucine)alterations at position 308 (open reading frame). Isoleucine distinguished between all Bali’s isolates andthe all of isolates from other area of Indonesia and other part of the world. This finding significantlydifferent as compared to other rabies virus isolates from other part of Indonesia or the world documentedon the GenBank. Accordingly it is proposed that it can be used as molecular marker and believed to be thefirst study of molecular marker of rabies virus in Indonesia.
Pemanfaatan Ekstrak Hipotalamus Kambing Sebagai Upaya Optimalisasi Kesuburan Kambing Kejobong Betina (THE USE OF GOAT HYPOTHALAMIC EXTRACT TO OPTIMALIZE FERTILITY OF FEMALE KEJOBONG GOATS) Zulkarnain .; Sutiyono .; Enny Tantini Setiatin
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to investigate the effect of hypothalamic extracts level on quality levelof estrous, pregnancy percentage and litter size of Kejobong goats. The materials used in this experimentwere the hypothalamus glands and 23 female Kejobong goats aged between 3-4 years. The experimentaldesign used in this study was a completely randomized design with 3 treatments 7-8 replicates. Thetreatment groups used in this study were T0 injected with 5 mL of 0.9% physiologic saline, T1 injected witha solution consisted of 2 mL of hypothalamic extract added 3 mL of 0.9% physiologic saline, T2 injectedwith solution consisted of 4 mL of hypothalamic extract added 1 mL of 0.9%physiologic saline. Theparameters were the percentage of estrous, estrous quality, onset of estrous, estrous length, pregnancypercentage and litter size. The data were analyzed by using analysis of variance and Chi-square. Theresults showed that hypothalamic extract was highly significant (P <0.01) affected the estrous length andhad no effect (P> 0.05) on the other parameters. In conclusion hypothalamic extracts may optimize thefertility of female Kejobong goats evaluated from estrous length, pregnancy percentage and litter size.
Efek Radiasi Sinar-X pada Ovarium-Uterus Tikus Putih (Rattus norvegicus) (THE EFFECT X-RAY RADIATION OF THE OVARIUM-UTERUS Rattus norvegicus) Pudji Rahardjo
Jurnal Veteriner Vol 1 No 1 (2000)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Efek Radiasi Sinar-X pada Ovarium-Uterus Tikus Putih (Rattus norvegicus)   (THE EFFECT X-RAY RADIATION OF THE OVARIUM-UTERUS Rattus norvegicus)
Aktivitas Penyembuhan Luka Sediaan Topikal Ekstrak Bawang Merah (Allium cepa) terhadap Luka Sayat Kulit Mencit (Mus Musculus) (THE ACTIVITY OF TOPICAL EXTRACT OF ONIONS (ALLIUM CEPA) ON WOUND HEALING PROCESS IN MICE (MUS MUSCULUS)) Visa Yunanda; Tristia Rinanda
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Wound healing is a physiological response of the body to restore continuity, structure and function ofthe injured tissue. Onion is one of the plants that are empirically used by the community to heal wounds.The purpose of this study was to observe the effect of topical extract of onions (Allium cepa) on woundhealing process in mice (Mus musculus) strain BalbC. This study used the posttest-only control groupdesign with completely randomized design (CRD). The samples were 12 male mice with weight ranged of22-32 g divided into four treatments; i.e.: Vaseline, topical extract of onion 5%, topical extract of onion30% and topical extract of onion 55% with 3 replications. Wound healing evaluated macroscopically toobserve hyperemia, wound contraction, granulation, crusting and pus production; and microscopically bycounting the number of fibroblasts on day 10. Hyperemia, granulation, crusting and pus production wereanalyzed descriptively. Wound contraction and the number of fibroblasts were analyzed using ANOVA(p<0.05) and followed with Duncan’s test (p<0,05). Descriptive observations obtained hyperemia lasteduntil day 3, granulation in wounds used topical extract of onion 30% and 55% were faster than the other,crusting lasted until day 6 and the production of pus was not found in any treatment. Statistical testresults showed that topical extract of onion 55% significantly affected wound contraction and topicalextract of onion 30% and 55% significantly affected maturation, which characterized by the decreasednumber of fibroblasts. The results of this study showed that topical extract of onion had significant effect(p<0.05) on wound healing and the effect was in line with the increased concentration of the onion extract.
Aktivitas Antipiretik Ekstrak Etanol Buah Belimbing Wuluh (Averrhoa bilimbi) pada Tikus Putih Jantan (ANTIPYRETIC ACTIVITY OF ETHANOL EXTRACT OF BELIMBING WULUH (AVERRHOA BILIMBI) IN MALE WHITE RAT) Andriyanto Andriyanto; Ni Made Ria Isriyanthi; Edwin Ligia Sastra; Ridi Arif; Aulia Andi Mustika; Wasmen Manalu
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.873 KB) | DOI: 10.19087/jveteriner.2017.18.4.597

Abstract

The experiment was conducted to study the activity of ethanol extract of belimbing wuluh (EEBW) as antipyretic. Fever induction was done by using difteri pertusis tetanus (DPT) vaccine intramuscularly at a dose of 0.1 mL/100 g BW. Male white rat strain spraque dawley with range of weight by 200 to 300 g was used in this research.Fifteen experimental rats were used to explore the specific time of fever (time of early fever and time of peak fever) which were divided in 2 treatments, i.e. 10 experimental rats with DPT vaccine injection and 5 experimental rats without DPT vaccine injection as control.Fourty experimental rats were used to study effectivity of EEBW as antipyretic which were grouped with factorial randomized design with 4 x 2 and 5 replications. The first factor was various substance administered which consisted of aquadest (control), EEBW 0.88 g/kg BW, EEBW 1.75 g/kg BW, and aspirin 0.004 g/kg BW.The second factor was time of fever detection, i.e. time of early stage fever and time of peak fever. The variable was measured with rectal temperature of the experimental rats by using digital thermometer (correction factor 0.01oC) every 30 minutes. The data were analyzed with t-student dan general linear model (GLM). Increasing of rectal temperature of experimental rats with DPT injection began at 30 minutes post-DPT injection (time of early stage fever) and reached time of peak fever at 210 minutes post-DPT injection. Ethanol extract of belimbing wuluh at dose of 1.75 g/kg BW administered at early fever lowered rectal temperature of the experimental rats compared to the other groups. It was concluded that the administration of EEBW at dose of 1.75 g/kg BW was effective combination in dose and time administration as an antipyretic.
Performance of Female Garut Lambs Fed Diet in Different Ratio of Protein and Total Digestible Nutrients (PERFORMANS ANAK DOMBA GARUT BETINA MASA PERTUMBUHAN YANG DIBERI PAKAN DENGAN IMBANGAN PROTEIN DAN TOTAL DIGESTIBLE NUTRIENT BERBEDA) Iman Hernaman; Budi Ayuningsih; Diky Ramdani; Mesa Maisarah; Siswoyo Siswoyo
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.228 KB) | DOI: 10.19087/jveteriner.2018.19.4.568

Abstract

The study aimed to study the effect of protein and total digestible nutrients on performance of female Garut lambs. A total of 20 female Garut lambs aged 7-8 months at averaged body weight 15.72 ± 1.33 kg were allocated randomly into four treatment diets with different crude protein and total digestible nutrients ratios. The treatments were: T1 = 11.53%:63.13%; T2 = 10.78%:70.06%; T3 = 13.54%:63.18%; and T4 = 13.67%:69.18%. Each treatment was replicated five times. The research was conducted experimentally for 12 weeks and the collected data were analyzed by Duncan’s multiple range test. The dry matter intake was significantly (p<0.05) higher in the treatments of T1 (707.70 g/d), T2 (677.71 g/d) and T4 (687.91 g/d) compared to T3 (587.01 g/d). The highest (p<0.05) average daily gain and the smallest (p<0.05) feed conversion ratio were significantly obtained by T4 (89.88 g/d and 7.75). Dry matter digestibility and organic matter digestibility was significantly (p<0.05) greater for T2 (63.93% and 64.10%) and T4 (59.73% and 65.14%) compared to T1 (49.98% and 55.34%) and T3 (44.68% and 50.60%) treatments. Based on the results, it can be concluded that T4 or the diet containing 13.67% crude protein and 69.18% total digestible nutrients yielded the best performance.
Fertilitas Semen Beku dalam Tris Kuning Telur dan Skim yang Diberi Omega-3 pada Sapi Simmental dengan Ransum Berimbuhan Seng dan Selenium Minimal Asep Kurnia; Soeparna Soeparna; Raden Iis Arifiantini; Rahmat Hidayat
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.337 KB) | DOI: 10.19087/jveteriner.2018.19.2.251

Abstract

This study aims to examine the quality of frozen semen in Tris egg yolk (TEY) extender and skimmed milk extender with or witout omega-3. A total of 18 Simmental bulls belong to Lembang Artificial Insemination Centre were divided into three groups. Each was fed with standard feed (R1), standard feeds supplemented with minimal Se and Zn (R2) and standard feed supplemented with maximal of Se and Zn concentration. Semen were collected using an artificial vagina and were evaluated macro- and microscopically. The semen then were divided into four tubes and each diluted with Skimmed, SkimmedOmega 3, TEY or TEY-O. The semen was then packed into a 0.25 ml straw and equilibrated at 5 oC for 4 hours, then frozen above liquid nitrogen vapor, and stored in liquid nitrogen container (-196 oC). The qualities of frozen semen were evaluated on the motility, individual score, viability and integrity of the plasma membrane of sperms. Sperm motility of bulls fed with standard feed (R1) in TEY extender and R3 in TEY and TEY-Omega-3 extender were higher (p <0.05) compared to the other combinations. No difference was found on the individual score. The viability of sperms in bulls fed with standard feed in SkimmedOmega-3 extender was higher than the other treatments and the highest sperm plasma membrane integrity was demonstrated by sperm in bull feeding with R2 in TEY extender.
Uji pigmen dan deteksi kapsul polisakarida pada Staphylococcus aureus isolat asal broiler PIGMENT TEST AND DETECTION OF POLISAKARIDA CAPSULES ON Staphylococcus aureus ISOLAT BROILER Khusnan Khusnan; Dwi Kusmanto
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.109 KB) | DOI: 10.19087/jveteriner.2019.20.3.369

Abstract

Staphylococcus aureus is a pathogenic bacterium causing disease in humans and animals. In broilers it cause septicemia, tendosinovitis, dermatitis, endocarditis, wound infections and arthritis and bumblefoot. The ability of Staphylococcus aureus to cause disease depends on the virulence factors they bear. The purpose of this research is to investigate the distribution of pigment production type and the existence of genes of polysaccharide capsule phenotype and genotype as determinant factor of virulence of bacteria on 15 isolates of Staphylococcus aureus from broiler. Pigment production test showed that 86.7% of isolates producing yellow pigment and 13.3% isolates produce orange pigment. The detection of polysaccharide capsules was phenotypically performed with hydrophobicity test with serum soft agar medium (SSA) showed 53.3% isolate grow compact and 46,7% isolate grown difuse and hydrophobicity test by salt aggregation test method (SAT) showed 66,7% hydrophil and 33.3% are hydrophobic. Genotype detection of polysaccharide capsule genes by polymerase chain reaction (PCR) showed 66.7% detected cap5 (amplicon 361bp) and 33.3% detected cap8 (173bp ampliole). The type of pigment production and the presence of polysaccharide capsules are some of the virulent factors in Staphylococcus aureus.

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