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Jurnal Biologi Udayana
Published by Universitas Udayana
ISSN : 14105292     EISSN : 25992856     DOI : https://doi.org/10.24843/jbiounud
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Jurnal Biologi Udayana (p-ISSN 1410-5292 | e-ISSN 2599-2856 | DOI 10.24843/jbiounud) managed by the Department of Biology, Udayana University, published in two formats namely print and online regularly twice a year (June and December).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 262 Documents
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IndonesiaDeteksi Escherichia coli O157 pada air minum di Kelurahan Sekaran Gunungpati Semarang Devi Dwi Jayanti; R Susanti; Ari Yuniastuti; I Wayan Suardana
Jurnal Biologi Udayana Vol 24 No 2 (2020): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2020.v24.i02.p01

Abstract

Penelitian bertujuan untuk mendeteksi adanya bakteri Escherichia coli O157 pada air minum kemasan, air minum isi ulang, dan air sumur di Kelurahan Sekaran Gunungpati Semarang. Sampel yang diambil sebanyak 20 sampel yang terdiri atas 4 merk air minum kemasan, 8 sampel air minum isi ulang, dan 8 sampel air sumur. Penelitian diawali dengan tahap isolasi E.coli pada medium Eosin Methylen Blue Agar (EMBA), yang dilanjutkan ke medium Sorbitol MacConkey Agar (SMAC) untuk identifikasi E.coli O157 dilanjutkan uji lateks aglutinasi (OXOID) dan diakhiri dengan uji konfirmasi gen rfbE menggunakan teknik Polymerase Chain Reaction (PCR). Hasil penelitian menunjukkan 8 sampel yang positif E.coli pada medium SMAC menunjukkan positif E.coli O157 (colorless). Uji lateks aglutinasi juga menunjukkan 8 sampel positif E.coli O157 seperti kontrol ATCC 43894. E.coli ATCC 43894 dan 8 sampel E.coli dari berbagai air minum di Kelurahan Sekaran Gunungpati Semarang menunjukkan positif E.coli O157.
Distribusi dan keanekaragaman jenis makroalga pada ekosistem lamun dan terumbu karang di pantai Desa Haya, Kabupaten Maluku Tengah, Provinsi Maluku Karel M Melsasail; Umar Namakule
Jurnal Biologi Udayana Vol 24 No 2 (2020): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2020.v24.i02.p08

Abstract

Haya merupakan salah satu desa di Kabupaten Maluku Tengah yang memiliki keanekaragaman jenis makroalga tersebar pada berbagai habitat dan belum teridentifikasi jenis serta sebarannya pada ekosistem lamun dan terumbu karang. Selain itu juga, keberadaan makroalga terancam oleh berbagai macam aktifitas masyarakat seperti pembuangan sampah dan limbah rumah tangga ke pantai, serta aktifitas nelayan menggunakan perahu yang merusak habitat makroalga. Dengan demikian peneliti merasa sangat perlu untuk melaksanakan penelitian ini. Tujuan dari penelitian ini untuk mengetahui distribusi dan keanekaragaman jenis makroalga pada ekosistem lamun dan terumbu karang di pantai desa Haya. Pengumpulan data dilakukan dengan metode transek liniear kuadrat. Transek yang digunakan sebanyak 5 buah dengan kuadrat berukuran 1x1 meter sebanyak 10 buah, serta didukung dengan pengukuran parameter fisika kima perairan seperti suhu menggunakan termometer, kuat arus menggunakan bola arus, kedalaman menggunakan tongkat berskala, salinitas menggunakan refaktometer, dan pH menggunakan pH meter. Jenis makroalga yang ditemukan di pantai Desa Haya sebanyak 12 jenis, 5 jenis pada ekosistem lamun dan 7 jenis pada terumbu karang. Distribusi makroalga pada ekosistem lamun dengan cara melekat pada substrat pasir dan lumpur, sedangkan pada terumbu karang dengan cara melekat dan menancap pada batu karang di rataan terumbu. Nilai keanekaragaman jenis makroalga pada ekosistem lamun tergolong rendah (1,381), sedangkan pada terumbu karang tergolong sedang (2,806). Kondisi faktor fisika-kimia perairan berupa suhu (29,2°C), kuat arus (23,8 cm/detik), kedalaman (124 cm), dan salinitas air laut (32,9 ppt) masih berada dalam kisaran yang baik untuk pertumbuhan makroalga, sedangkan derajat keasaman (pH) air laut (6,07) berada dalam kisaran yang tidak baik bagi perumbuhan makroalga.
Efek pemberian ekstrak kedelai hitam (Glycine soja) terhadap histologi femur pada mencit (Mus musculus) ovariektomi mahhriani mahriani; Resa Miftahatu Yuniar; Masrurotul Hasanah; Eva Tyas Utami
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p05

Abstract

Bone is a dynamic structure and has the process to remodelling or regeneration. Bone remodelling includes the process of formation by osteoblasts and the process of resorption by osteoclasts. The balancing between formation and resorption are assisted by the estrogen hormone.Estrogen deficiency can lead the bone resorption. Ovariectomy is a model for estrogen deficiency by removing the ovaries. One way to overcome estrogen deficiency can be solved by offering phytoestrogens. This research have purpose to determine the effect of black soybean extract (Glycine soja) on the histology of the femur mice (Mus musculus) ovariectomized. In this research used a dose of black soybean extract as many as 0.42 g /ml/day and 0.63 g/ml/day, which are given orally for 30 days. The research is conducted with a completele randomized design with 4 treatments and 6 replications. Observations are made on the number of osteoblasts and the number of osteoclasts on the histological of the femur. The results showed that offering black soybean extract at a dose of 0.42 g/ml/day for 30 days have significantly increased the number of osteoblasts and have decreased the number of osteoclasts in the femur of ovariectomized mice.
Pengelolaan sampah anorganik pada Alila Villas Uluwatu, Bali Sulfa Ilmiyah; A.A.G. Raka Dalem; I Ketut Muksin
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p09

Abstract

Environmental problems are complex things that they are very influential on all components in the ecosystem. One of the environmental problems is caused by the production of waste that is not treated properly, for example in the hotel industry. Tourism is one of the activities that produce various kinds of waste, both environmentally friendly and not. Products that are not environmentally friendly can pollute the environment if they do not handled properly. Alila Villas Uluwatu (AVU) is one of hotels in Bali that are committed to environmentally friendly and efforts to protect the environment by minimizing waste production. The objective of this research was to find out AVU's strategies in protecting the environment, especially in handling the resulting inorganic waste, such as glass, paper and plasctics. The method utilized in data collection on this study was through conducting interview, direct observation in the field and examination of related documents. Data analysis was performed descriptively, either in qualitative and quantitative forms. The results showed that the waste management strategy in AVU included efforts to minimize inorganic waste production and by using more environmentally friendly materials such as toothbrushes with wooden handles, paper straws and bamboo straws.
Penapisan toleransi kekeringan padi lokal Lampung pada fase perkecambahan Lili Chrisnawati; Yulianty Yulianty; Eti Ernawiati; Umilia Fitriyani; Aprilia Eka Putri
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p01

Abstract

Seed germination is one of the most critical stages in plant growth. Screening at this stage can provide preliminary information on drought tolerance. This study aims to evaluate drought tolerance in local Lampung rice varieties Lumbung Sewu Cantik in the germination phase. This study used a completely randomized design with three concentrations of PEG and three replications. The PEG concentrations given were 0%, 10%, and 15%. The observed data were analyzed for variance at the level a test = 0.05 and further analysis used Duncan's Multiple Range Test (DMRT). The results showed that the higher the PEG concentration, the plumulae length and seminal root length of rice decreased. However, in radicles, PEG 20% did not decrease length growth. Giving PEG 20% also did not decrease the dry weight of the radicles and in the seminal roots, there was no significant difference in weight between PEG 10% and PEG 20%. This indicated that Lumbung Sewu Cantik Padi has the potential to tolerate drought in the germination phase.
DNA barcoding pada invertebrata laut Agus Mohammad Hikam; Nurul Jadid Mubarakati; Muhammad Dailami; Abdul Hamid A. Toha
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p06

Abstract

A large number of species of marine invertebrates that have similar morphology cause misidentification very possible. Molecular species identification is important in taxonomy study accurately. This study aims to determine the identity of Aquatic Papua marine invertebrates with DNA barcoding technique using markers COI gene (Cytochrome c oxidase I). The study identified 29 individuals of marine invertebrates, which came from sea cucumbers (6 samples), lobsters (6 samples), octopus (6 samples), chitons (5 samples), and sea urchins (6 samples). Molecular identification methods consist of DNA isolation, PCR COI gene fragments, sequencing, and DNA sequence analysis using bioinformatics software. The results showed that the sea cucumber came from the Bohadschia marmorata species, the lobster came from the Panulirus versicolor species, the octopus came from the Octopus cyanea species, the chiton came from the Ischnochitonaustralis species, and the sea urchins came from the Tripneustesgratilla species. The similarity of samples and references are in the range of 84.58 to 100.00%. The disparity index, genetic distance, and phylogenetic tree supported this result.
Histomorfometrik duodenum kelinci jantan setelah diberi pakan komersial disuplementasi minyak hati ikan kod Ni Gusti Ayu Manik Ermayanti; Ni Wayan Sudatri; Ni Nyoman Wirasiti; I Gusti Ayu Manik Widhyastini
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p10

Abstract

Fish oil is a source of long-chain polyunsaturated fatty acids (LCPUFA) that can be used as supplementary feed in commercial feed to increase male rabbit productivity. PUFAs function in various physiological processes, including in the digestive system.This study aimed to determine the histomorphometrics of male rabbits duodenum after fed commercial feed supplemented by cod liver oil. The experimental design used was a Completely Randomized Design (CRD) with four feed packages, namely commercial feed without cod liver oil (P0) as a control, commercial feed supplemented with cod liver oil 3% (P1), 4.5% (P2) and 6% (P3). Each consists of five male local rabbits as replicates and given starting female rabbits 1-3 months. The parameters observed were villi height, villi width, crypt depth, and tunica mucosa thickness of the duodenum in male rabbits. The data obtained were analyzed with Anova and if significantly different were further tested with DMRT. The results showed that cod liver oil supplementation in commercial feed was significantly different (p<0.05) to the histomorphometric parameters (villi height, villi width, crypt depth, and thickness of the tunica mucosa) of the duodenum in male rabbits. Can be concluted that supplementation of cod liver oil in commercial feed can increase the histomorphometrics of male local rabbits duodenum.
Karakter morfologis dan molekuler udang regang (Macrobrachium sintangense De Man, 1898) dari Daerah Istimewa Yogyakarta Rini Rahmawati; Rury Eprilurahman; Lukman Hakim; Tuty Arisuryanti; Zuliyati Rohmah; Trijoko Trijoko
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p02

Abstract

Macrobrachium genus has many species members and distributed in various countries including Indonesia. One of the species belonging to the Macrobarachium genus that can be found in Indonesia was Macrobrachium sintangense or known as Sunda River Prawn. Identification of this species is important as an effort to expand studies on Sunda River Prawn in the Region of Yogyakarta. Identification of this species was carried out morphologically and molecularly with the 16S gene. The purpose of this study was to determine the morphological and molecular characters of M. sintangense. Morphological identification is done by identifying morphological, morphometric, and meristic characters according to the identification key. Molecular analysis was carried out by isolation, amplification, electrophoresis, and DNA visualization with UV illuminators, DNA sequencing by 1st Base. The results of this study indicate the morphological character of the rostrum slightly curved upwards or like a knife, rounded post antennular carapace, absent preanal carina, pubescene in the proximal half to two thirds of the chela. Morphologically, all samples were identified as members of the genus Macrobrachium. There are eight (8) specimens identifiable to the species level, as M. sintangense. The results of molecular identification of two (2) specimens showed a high similarity value and a close relationship with M. sintangense from GenBank samples and also close relationship with M. sintangense from Tukad Panti, Bali, Indonesia. The conclusion of this research is that morphologically and molecularly, the samples found in the Winongo.
Karakter morfologis dan molekular udang air tawar anggota Macrobrachium pilimanus species group di Sungai Opak, Winongo dan Sempor, Daerah Istimewa Yogyakarta Malya Adzillina Silmi; Rury Eprilurahman; Trijoko Trijoko; Lukman Hakim
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p07

Abstract

Macrobrachium is a genus of the Crustacea subphylum with the highest diversity. Identification of the morphology of the genus Macrobrachium is mostly challenging due to the characters for species level identification are complicated, whereas many characters are common to all Macrobrachium species. In addition, it is difficult to identify morphology because of the influence of social dominance in males. Therefore, another approach is needed to identify Macrobrachium accurately, one of which is by barcoding DNA using the 16S mitochondrial gene. This study was aimed to determine the morphological and molecular characters of Macrobrachium pilimanus species group based on the 16S mitochondrial gene from the Opak, Winongo, and Sempor rivers, in Daerah Istimewa Yogyakarta. Furthermore, shrimp samples were identified based on morphological, meristic, and morphometric characters. Meanwhile, molecular analysis uses 16Sar as forward primer and 16Sbr as reverse primer. Based on the morphological identification results, the shrimp samples obtained could only be identified up to the level of the Macrobrachium genus. In addition, the shrimp samples can also be included in the Macrobrachium pilimanus species group based on the general characteristics of the species group. The shrimp samples in this study have highest similarities in character with the species M. pilimanus and M. empulipke distributed in Java. Based on molecular analysis, sample from Winongo River (WNM1) has a similarity of 87.63% with Macrobrachium forcipatum (JQ362454.1) and 87,02% with M. dienbienphuense (JQ390474.1), which means that it is a different species from the species in the GenBank. Macrobrachium forcipatum and M. dienbienphuense are members of M. pilimanus species group. The conclusion of this research is that all the samples are member of M. pilimanus species group.
Meta-Analisis: Efektifitas pemberian pakan buatan untuk meningkatkan laju pertumbuhan dan kualitas reproduksi spesies Monopterus albus Tomi Apra Santosa; Rani Dwi Suci Hd Putri; Ramadhan Sumarmin; Dwi Hilda Putri; Abdul Razak
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p11

Abstract

The use of artificial feed for fish farming has been widely used at this time, however, it is not yet known the significant effect of artificial feed on the growth and reproduction rate of Monopterus albus. This study aims to determine the effectiveness of artificial feeding on the growth and reproduction rate of Monopterus albus species. This research is meta-analysis research. The data source comes from a search of 18 national and international articles published in 2010-2020 related to feeding the Monopterus albus species. Data obtained from google scholar database, DOAJ, ScienceDirect, sagejournal, Springer, and IEEE. The data analysis technique is a qualitative descriptive analysis with JASP software. The results showed that 35% of artificial feeding affected the growth of Monopterus albus and 28.5% affected the reproductive rate with an effect size of 1.2. This shows that artificial feeding has an effect of 0.334 in increasing the growth and reproductive quality of Monopterus albus.

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