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All Journal Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Lingkungan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Jurnal Biologi Tropis Jurnal Biogenerasi BIO-EDU: Jurnal Pendidikan Biologi Biofarm : Jurnal Ilmiah Pertanian BIOVALENTIA: Biological Research Journal Kenanga : Journal of Biological sciences and Applied Biology Journal Of Medicine And Health Sciences (Medisci) Journal Of Agriculture Sciences (Agrosci) Journal of Community Service (Abdisci)
Pratiwi, Dian Neli
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Oral Swab-Based DNA Extraction Of Cynopterus Brachyotis: An Initial Step For Species Confirmation Rhamadaningtyas, Nabila Aulia; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Srihanto, Eko Agus; Saswiyanti, Enny; Pratiwi, Dian Neli; Susanto, Alvin Wiwiet
Jurnal Agrosci Vol 3 No 3 (2026): Vol 3 No 3 January 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i3.1025

Abstract

Background. Identifying bat species based on morphological characteristics often faces challenges due to character overlap among species, especially within cryptic species groups. Cynopterus brachyotis is one of the fruit bats with high genetic diversity but relatively uniform morphology. Aims. Therefore, a molecular approach is needed to support accurate species confirmation. Under the HETI Research Grant of Innovation and Collaboration Batch 3- II Year 2025, this study aims to obtain genomic DNA from C. brachyotis bats caught at the Lampung Disease Investigation Center as the initial step of molecular species confirmation. Methods. Samples were obtained by taking oral swabs on bat individuals caught using mist nets. DNA extraction is performed using silica membrane-based methods with commercial kits. Quantitative evaluation of DNA was performed using a Qubit Fluorometer, while qualitative evaluation was performed by agarose gel electrophoresis. Conclusion. The results showed that the DNA concentration was in the low range (0.1–0.7 ng/μL), with a DNA band appearing very thin on electrophoresis. Implementation. Nevertheless, the quantification results confirm that DNA was successfully extracted and remains suitable for advanced molecular analysis, with optimization at the DNA amplification stage.
Learning Bats Handling For Oral Swab Sampling Azmi, Sevira Nur; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Saswiyanti, Enny; Srihanto, Eko Agus; Pratiwi, Dian Neli; Susandi, Waryoko
Jurnal Medisci Vol 3 No 1 (2025): Vol 3 No 1 August 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/medisci.v3i1.1014

Abstract

Background. The only flying mammals, bats, play an essential role in their natural habitat. Based on the type of food, bats can be divided into two groups: fruit-eating bats and insect-eating bats. Bats rank second-highest in species diversity, with 1,439 worldwide and 239 in Indonesia. Known as reservoirs of viruses, including coronaviruses. Aims. Bats are suspected to be related to COVID-19. Under the Research Innovation and Collaboration Program - Higher Education for Technology and Innovation Project (HETI) University of Lampung 2024-2025, and in collaboration with the Lampung Disease Investigation Centre, learning the procedures for handling bats properly so as not to physically harm bats in oral swab sampling and species recognition in Braja Harjosari, directly next to Way Kambas National Park was done. Methods. The life-trapping technique uses a mist net. Bat handling for taking oral swab was carried out using the pinch grip method, which is holding both arms of the bat backwards utilizing the thumb and middle finger with the bat positioned facing upwards. Conclusion. The bat's mouth is then blown open and a cotton swab is gently inserted into the bat's mouth. Afterwards, the bat is rested, given water drops, and released into nature. Oral swab samples from 10 individual bats: frugivorous bats, Cynopterus brachyotis (n = 8), Cynopterus horsfieldii (n = 1), and insectivorous bat, Scotophilus kuhlii (n = 1).
Co-Authors Adelia, Ersa Imelda Afandi, Aril Agus Subagyo Al Amin, Muhammadi Ridho Alfandi, Aril Alimudin, Salih Angga Kurniawan Ariyanto, Slamet Puji Arum, Setiawan Ashari, Minanti Mayda Azmi, Sevira Nur Azzahra, Laila Salwa Bayu , Raden Fadli Dwi Asmi Dwitama , Harry Putra Edi Santoso Eko Agus Srihanto, Eko Agus Elly Lestari Rustiati Elly Lestari, Rustiati Erdi Suroso Fahrezi, Aditya Febriansyah, Muhammad Febriyani, Dwi Ayu Firwantoni, Firwantoni Gina Dania Pratami, Gina Dania Imtitsal, Aulia Indah Ayu Lestari, Indah Ayu Jani Master, Jani Krismuniarti, Elisabeth Devi Kusuma, Andriyani Wijaya Lestari, Septi Wahyu Maharani, Annisa Lidya Novianasari, Tika P. Haryanto, Sugeng Permatasari, Nindy Priyambodo Priyambodo Priyambodo Priyambodo Priyambodo Raden Mohamad Herdian Bhakti Ramadan, Viki Ramadhan, Viki Rhamadaningtyas, Nabila Aulia Rustiaati, Elly Lestari Sa'uddah, Lousanja D. Samsul , Bakri Sandora, Desta Sandra, Shifa Saswiyanti, Enny Sendora, Desta Septiana, Ana Sidik, Mahfud Sri Retnaning Rahayu, Sri Retnaning Suhada Suhada, Suhada Sunandar Sunandar Supriyadi, Tatang Susandi, Waryoko Susanto, Alvin Wiwiet Suwarno Thesalonika, Natasya Virnarenata, Elsa Wahyono, Agus WINARNO Winarno Winarno Winda, Indriyanti Yani, Ani Andri Yanti Yulianti Yanti, Yulianti Yuliana Andriyani