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All Journal Jurnal Ilmu Informasi Perpustakaan dan Kearsipan Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Jurnal Medika Veterinaria
Legowo, Djoko
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Library & Information Science Medicine & Pharmacology Veterinary

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Effect of Red Dragon Fruit Extract (Hylocereus polyrhizus) on MDA Metabolite Products and CD36 Expression in Artery Carotid Communis Mice (Mus muscullus) Fed a High-Fat Diet Larasati, Galuh Wiedani Kusuma Dyah; D.K. Wardani, Lailia; Legowo, Djoko
Jurnal Medika Veterinaria Vol 18, No 1 (2024): J.Med.Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v18i1.35653

Abstract

Red Dragon fruit (Hylocereus polyrhizus) has the potential for the prevention of diseases associated with inflammatory and oxidative processes such as cardiovascular diseases due to the presence of their antioxidant compounds including phenols, flavonoids, tocopherol, ascorbic acid, thiamin, niacin, riboflavin, minerals, betacyanin, -carotene, lycopene, p-coumaric acid, protocatechuic acid, vanillic acid, gallic acid, syringic acid, and p hydroxybenzoic acid. Antioxidant compounds can prevent the atherogenesis process by reducing MDA metabolite products and CD36 expression in artery carotid communis. MDA metabolite products are indicators of lipid peroxidation, while CD36 expression is a scavenger receptor which responsible for the retention and absorption of LDL-Ox in macrophages and endothelial cells. This study aimed to prove the effect of red dragon fruit extract in reducing MDA metabolite products and CD36 expression in artery carotid communis of mice fed a high-fat diet. This research was in vivo experimental study with a randomized post test only control group design. This research used 40 male mice which were divided into five groups, namely negative control K0 (without high fat diet and extract), positive control K1 (high fat diet without extract), P1 (5mg extract/mice/day), P2 (extract 10mg/mice/day), P3 (extract 20mg/mice/day). A high fat diet was given for 56 days. Red dragon fruit extract was given for 28 days. At the end of the study, all mice were euthanized and their artery carotid communis were taken for immunohistochemistry staining. MDA and CD36 expression measurements were carried out using the Immune Reactive Score (IRS), which is the result of multiplying the percentage of immunoreactive cells (A) with the color intensity score (B) of immunoreactive cells.
The effect of epigallocatechin 3-gallate (EGCG) on Sertoli and Leydig cells counts and spermatozoa quality of rats (Rattus norvegicus) exposed to monosodium glutamate Indira, Sitti Az-Zahra; A Ashiila Tsabita; susilowati, Suherni; Sri Pantja Madyawati; Yudaniayanti, Ira Sari; kusnoto, kusnoto; Legowo, Djoko; Hidajati, Nove; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 15 No. 1 (2026): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v15i1.2026.56-64

Abstract

This study aimed to determine the preventive effect of epigallocatechin 3-gallate (EGCG) on the number of Sertoli and Leydig cells in white rats (Rattus norvegicus) exposed to monosodium glutamate (MSG). This study used 25 male rats which were divided into five groups. The C– group was given only 1% Na-CMC, while the C+ group received 1% Na-CMC and 120 mg/kg BW of MSG. The T1, T2, and T3 groups were administered EGCG at doses of 4, 8, and 16 mg/kg BW, respectively, along with 120 mg/kg BW of MSG. All treatments were administered orally for 28 days. After the treatment period, the rats were sacrificed on day 29. Their testes were collected and processed into histological specimens using hematoxylin eosin (H&E) staining. The results showed that MSG exposure significantly decreased (p <0.05) the number of Sertoli and Leydig cells in the C+ group compared to the C– group. In contrast, the T1, T2, and T3 groups showed increased cell counts following EGCG administration. Epigalocathecin-3-gallate at a dose of 8 mg/kg BW showed the best potential in maintaining spermatozoa motility, viability, and plasma membrane integrity in rats exposed to MSG. EGCG, as an antioxidant, can reduce lipid peroxidation in the spermatozoa plasma membrane, thereby improving spermatozoa viability, motility, and plasma membrane integrity. In conclusion, the administration of EGCG mitigates the harmful effects of MSG on Sertoli and Leydig cell counts in white rats, with an optimal dose of 8 mg/kg BW.
Co-Authors A Ashiila Tsabita Ahmad Saeroji Amaris, Aulia Puspa Andriani, Ratna Arimbi Boedi Setiawan Chairul Anwar Charles, Vanessa Ann Chusniati, Sri D.K. Wardani, Lailia Dadik Rahardjo, Dadik Darmawan, Moch. Rizky Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Suprihati Hani Plumeriastuti Hayuning Nurrodhiya Hidajati, Nove Indira, Sitti Az-Zahra Ira Sari Yudaniayanti Ismiyati, Ismiyati Ismudiono Ismudiono, Ismudiono Kadek Rachmawati Kadek Rachmawati Kuncorojakti, Suryo Kurniati, Yuniar Kusnoto Kusnoto, Kusnoto Larasati, Galuh Wiedani Kusuma Dyah Lilik Maslachah Mochamad Lazuardi Mufasirin Nusdianto Triakoso Permatasari, Roselia Yuliani Poedji Hastutiek Prakosa, Dwi Untung Pujiyanto, Mahendra Rendragraha, Briantono Willy Rochmah Kurnijasanti Sholikah, Mar'atus Sri Pantja Madyawati Suherni Susilowati Suzanita Utama Tita Damayanti Lestari Tyasningsih, Wiwiek Wahyuni, Retno Sri Xin, Irene Teh Kai Yustinasari, Lita Rakhma Zainal Arifin