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All Journal Meditory : The Journal of Medical Laboratory Jurnal Analis Medika Biosains (JAMBS) JPP Jurnal Kesehatan Poltekkes Palembang Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Kesehatan Tambusai Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Indonesian Health Issue Jurnal Laboratorium Khatulistiwa Journal of Indonesia Laboratory Students (JILTS) Jurnal Indonesia Mengabdi Galen: Jurnal Ilmu Farmasi dan Kesehatan
Thomas Tandi Manu
Jurusan TLM Poltekkes Kemenkes Mataram
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Genetic Diversity of Plasmodium falciparum Glutamate Rich Protein in Patients Attending the Merauke Hospital in Papua Province, Indonesia Thomas Tandi Manu; Puspa Wardhani; Heny Arwati; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 27, No 2 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i2.1662

Abstract

Malaria remains an important health problem in Indonesia with the highest transmission in Papua Province, an easternpart of this country. The genetic diversity of malaria parasites is the main problem in understanding several aspects ofmalaria infections and the dynamics of their transmission, which also play a role in the development of a vaccine.Plasmodium falciparum is the deadliest of the human malaria parasites. Plasmodium falciparum glutamate-rich protein(Pfglurp) is one of the many erythrocytic stages antigens currently under development for a vaccine. The Pfglurp gene hasbeen extensively used as a marker to investigate the genetic diversity, Multiplicity of Infection (MOI), the level of malariatransmission, immunity against malaria, as well as a discriminatory instrument to distinguish new from recrudescentinfections of the field parasite population. Thus, this genotyping study aimed to find out the genetic population ofP.falciparum at the Merauke District, Province of Papua, Indonesia. DNA samples were isolated from Dried Blood Spots(DBS) obtained from P.falciparum infected patients in the Regional Public Hospital of Merauke, Province of Papua, Indonesiaduring May 2019-July 2019. The isolated DNAs were then amplified for nested Polymerase Chain Reaction (PCR) prior toPfglurp genotyping. The glurp gene was identified in all 51 DBS samples of P.falciparum-infected patients, and 18 variants ofallele were found. Among them, 45.10% were found to bear multigenotype infections. The size of the dominant allele(12.5%) was 701-750 bp. The MOI was 1.58. The genetic population of P.falciparum in Merauke Hospital has contained ahigher percentage of multigenotypes compared with monogenotypes indicating the high transmission of malaria in thestudied area.
GAMBARAN KADAR ASAM URAT DARAH KARYAWAN POLITEKNIK NEGERI INDRAMAYU Sari Artauli Lumban Toruan; Thomas Tandi Manu; Putri Sukma Diani
Jurnal Kesehatan Tambusai Vol. 3 No. 4 (2022): Desember 2022
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v3i4.8272

Abstract

Salah satu Penyakit Tidak Menular adalah penyakit sendi, bedasarkan hasil survey yang dilakukan WHO pada tahun 2017 Indonesia merupakan negara terbesar ke 4 didunia yang penduduknya menderita asam urat. Gejala klinik penyakit sendi berupa gangguan nyeri pada persendian yang disertai kekakuan, merah dan pembengkakan yang bukan disebabkan karena benturan/ kecelakaan dapat berlangsung kronis, salah satu penyebab dari penyakit sendi adalah tingginya nilai asam urat dan salah satu factor penyebabnya adalah aktifitas fisik yang tidak seimbang dengan pola makan sehat. Penelitian ini dilakukan untuk mengetahui gambaran hiperuresemia pada karyawan Politeknik Negeri Indramayu. Jenis penelitian ini adalah deskriptif dengan desain potong lintang. Populasi penelitian ialah karyawan tetap Politeknik Negeri Indramayu, pengambilan sampel dilakukan dengan metode total sampling dengan responden berjumlah 30 reponden dan kriteria inklusi yaitu pegawai aktif di Politeknik Negeri Indramayu dengan lama kerja kurang lebih 8 jam. Berdasarkan hasil penelitian didapatkan sebagian besar responden memiliki kadar asam urat darah normal dan menunjukkan bahwa perempuan lebih banyak memiliki kadar asam urat normal dibandingkan laki-laki. Berdasarkan penelitian ini dapat disimpulkan sebagian besar karyawan Politeknik Negeri Indramayu memiliki kadar asam urat dalam batas normal.
PEMANFAATAN AIR KELAPA MUDA SEBAGAI MEDIA ALTERNATIF MAC CONCEY UNTUK PERTUMBUHAN Escherichia coli DAN Salmonella typhi Sari Artauli Lumban Toruan; Thomas Tandi Manu; Paulina Rosa Evriarti
Journal of Indonesian Medical Laboratory and Science Vol 4 No 1: April 2023
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v4i1.143

Abstract

The growth of microorganisms requires media as a material consisting of a mixture of nutrients. used to grow microbes This study is laboratory experiment that aims to determine the protein composition on an old coconut water which is used as an alternative Mac Conkey Agar that can be used for growth of Escherichia coli and Salmonella typhi to characterized by non fermenter lactose and fermenter lactose colony. Research method is experimental test using inoculated on alternative media and Mac Conkey Agar as a control. Old coconut water media composition composed resemble for Mac Conkey Agar and media control that the order for Mac Conkey Agar. The bacterium is Escherichia coli and Salmonella typhi as test bacteria fermenter non fermenter lactose and lactose. Analysis of data using T test results showed an old coconut water alternative media can foster better Escherichia coli when compared to Salmonella typhi, because there is no difference in the number and diameter of colonies significantly to media controls.  In the alternative media do not growth old coconut water Salmonella typhi the same controls. Based on the results of the research can be concluded that Escherichia coli and Salmonella typhi can grow on alternative media of Old Coconut Water.
UJI KUALITATIF ALKOHOL URINE PEMINUM TUAK DALAM MENGETAHUI WAKTU SAMPLING YANG TEPAT I Made Suardika; Lale Budi Kusuma Dewi; Thomas Tandi Manu; I Wayan Getas; Ida Bagus Rai Wiadnya
Meditory : The Journal of Medical Laboratory Vol 11, No 1 (2023): Meditory, Volume 11 No. 1 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i1.2421

Abstract

Background:Accidents and criminal acts can be caused by alcohol use. Alcohol use by a person can be known by analyzing a urine sample. In the analysis of urine samples it is necessary to take the right sampling time. In addition, another factor to consider when detecting alcohol in urine is the time of last consumption. Aims:This study was conducted to determine the appropriate urine sampling time for alcohol consumption. Methode:The method used in this urine alcohol qualitative test uses the modified 2.5% potassium bichromate method in 50% H2SO4. The sample consisted of 10 respondents who drank palm wine after 1 hour, 2 hours and 3 hours of consumption. The results:The qualitative test results of urine drinkers of palm wine drinkers after 1 hour, 2 hours and 3 hours of consuming. 10 respondents obtained the results of 4 positive urine samples 1 hour after consuming and 2 positive urine samples 3 hours after consuming palm wine, as well as sampling in the 2nd hour the test results were negative. Conclusion:The positive test results depended on the habit of consuming, the volume consumed, and the age of the wine drinker
Genetic Diversity of Plasmodium falciparum Glutamate Rich Protein in Patients Attending the Merauke Hospital in Papua Province, Indonesia Thomas Tandi Manu; Puspa Wardhani; Heny Arwati; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 27 No. 2 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i2.1662

Abstract

Malaria remains an important health problem in Indonesia with the highest transmission in Papua Province, an eastern part of this country. The genetic diversity of malaria parasites is the main problem in understanding several aspects of malaria infections and the dynamics of their transmission, which also play a role in the development of a vaccine. Plasmodium falciparum is the deadliest of the human malaria parasites. Plasmodium falciparum glutamate-rich protein (Pfglurp) is one of the many erythrocytic stages antigens currently under development for a vaccine. The Pfglurp gene hasbeen extensively used as a marker to investigate the genetic diversity, Multiplicity of Infection (MOI), the level of malaria transmission, immunity against malaria, as well as a discriminatory instrument to distinguish new from recrudescent infections of the field parasite population. Thus, this genotyping study aimed to find out the genetic population of P.falciparum at the Merauke District, Province of Papua, Indonesia. DNA samples were isolated from Dried Blood Spots (DBS) obtained from P.falciparum infected patients in the Regional Public Hospital of Merauke, Province of Papua, Indonesiaduring May 2019-July 2019. The isolated DNAs were then amplified for nested Polymerase Chain Reaction (PCR) prior to Pfglurp genotyping. The glurp gene was identified in all 51 DBS samples of P.falciparum-infected patients, and 18 variants of allele were found. Among them, 45.10% were found to bear multigenotype infections. The size of the dominant allele (12.5%) was 701-750 bp. The MOI was 1.58. The genetic population of P.falciparum in Merauke Hospital has contained a higher percentage of multigenotypes compared with monogenotypes indicating the high transmission of malaria in the studied area.
Correlation Of Positive IgM or IgG RDT (Rapid Diagnostic Test) Results with CRP (C-Reactive Protein) Levels in Dengue Hemorrhagic Fever Patients Yaumi Tasu’a Salsabila; Pancawati Ariami; Ida Bagus Rai Wiadnya; Thomas Tandi Manu
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.319

Abstract

Background: Laboratory diagnosis of Dengue Hemorrhagic Fever includes the IgM & IgG RDT (Rapid Diagnostic Test) examination which differentiates primary and secondary dengue infection. The presence of inflammation in the body caused by the dengue virus needs to be examined for C-Reactive Protein. Methods: This research is an analytical observational study with a cross sectional approach. Thesample used is a plasma sample of a patient diagnosed with DHF with a positive IgM or IgG RDT result. Results: Positive IgM RDT results were 6 people (17%) and positive IgG were 30 people (83%). The results of a qualitative CRP study were 24 people (67%) with positive CRP levels. Semi-quantitative CRP average level for positive IgG was29.33. The results of the Spearman's Rank statistical test obtained a value of p = 0.003 <0.05 which showed a significant result. Conclusion:Clinically it shows that the more positive IgM or IgG RDT results obtained, the higher the CRP level which indicates the severity/inflammation that is formed in the body.
Correlation of Leukocyte Esterase Positivity Levels Against the Results of Several Examination Parameters Laboratory Urinary Tract Infection in Patients at Mataram City Regional Hospital I Made Wahyu Waisnawa; Ida Bagus Rai Wiadnya; Thomas Tandi Manu; Agrijanti Agrijanti
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.326

Abstract

Background : Urinary tract infection (UTI) is caused by the multiplication of bacteria in the urinary tract. The standard proving procedures for UTI are urine culture examination and urine dipstick examination (leukocyte esterase and nitrite). Objective : This study aims to measure leukocyte and nitrite values, calculate the total number of bacteria, identify Gram type, species and antibiotic sensitivity and analyze the correlation of leukocyte esterase positivity to the results of several UTI examination parameters. Research Methods : The method used is an analytic observational design with cross sectional design using primary data and analyzed with the Spearman rank test. Results : The results showed that in 198 patients with suspected UTI, 123 (62%) were positive for leukocyte esterase, 16 (8.1%) were positive for nitrite. The total number of bacterial countshowed 82 people (41%) with bacteriuria ≥10 5 CFU/mL. Found 61 Gram-negative bacilli and 21 Gram-positive cocci with 21 different bacterial species. Antibiotic sensitivity test showed 51% sensitive, 6% intermediate and 43% resistant. Correlation of leukocyte esterase with nitrites (p 0.023<0.05), total bacteria count (p 0.00<0.05), Gram negative bacilli (p 0.00<0.05), and Gram positive cocci (p 0.533>0.05), antibiotic sensitivity test (p 0.794 >0.05) and bacterial species (p 0.316>0.05). Conclusion : The results showed that there was a correlation between the level of positivity of leukocyte esterase and nitrite, total bacteria count and Gram-negative bacilli, but there was no correlation between Gram-positive cocci, bacterial species and antibiotic sensitivity tests.
Utilization Of Kuku Pancar (Lawsonia Inermis Lin) Leaves as a Substitute For Eosin Dye in The Examination of Soil Transmitted Helminth (STH) Eggs Lala Deswinta Yusari; Lalu Srigede; Thomas Tandi Manu; Ari Khusuma
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.356

Abstract

Nail surfacing leaves (Lawsonia inermis lin) are natural materials commonly used as dyes, because of the content of chemical compounds in them such as lawsone, flavonoids, tannins and anthocyanins, therefore nail surfacing leaves can be used as an alternative dye to replace 2% Eosin in STH worm egg examination, because it has the same properties as 2% Eosin and can produce an orange yellow color after the extraction process. The research objective is to determine the results of nail surfacing leaves (Lawsonia inermis lin) can be used as an alternative substance to replace Eosin in the examination of STH (soil transmitted helminth) worm eggs. The methods of this research is using is experimental, where this study looks at the clarity of soil transmitted helminth (STH) worm eggs on preparations, using coloring from the results of soaking the leaves of the nail surf as a substitute for Eosin. The results of the research is that the coloring results obtained in each variation of immersion time are good. The conclusion of this research is that the staining of worm eggs using the results of immersion of nail surfacing leaves for 12 hours, 24 hours, 36 hours, and 48 hours can be used as an alternative dye in the examination of STH worm eggs.
Gambaran Jumlah Leukosit Pada Penderita Suspek So TB Paru di Rumah Sakit Patuh Patut Patju Reginata, Ni Luh Vira; Rohmi, Rohmi; Manu, Thomas Tandi; Khusuma, Ari
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1453

Abstract

Leukocyte examination is carried out to describe disease events or processes in the body, aspecially infectious diseases such as tuberculosis bacteria can attack immune system cells, including leukocytes. Bacteria can survive and reproduce in leukocytes. Pulmonary TB sufferers before treatment saw an increases in leukocyte levels, indicating the formation of many leukocytes to fight bacteria in the phatocytosis process. On of the laboratory tests used to support the diagnosis of tuberculosis is an examination of the leukocyte count. This study aimed to determine the description of the number of leukocytes in patients with Suspected SO Pulmonary TB at The Patuh Patut Patju Hospital Untuk. This study design is descriptive case study research. Sampling technique was carried out using accidental sampling technique and  30 respondents obtained. The results of this study showed that the average leukocyte levels of patients with suspected SO Pulmonary TB were 9,643/μl, with 13 people (1+), 12 people (2+), 5 people (3+). The highest average leukocyte count was found in the female gender, age group 56-65 years and on 5 months treatment
Perbedaan Jenis Spesimen Urine Terhadap Hasil Pemeriksaan Kimiawi Urine Metode Carik Celup Baiq Eliya Yusrina Eliya; Yunan Jiwintarum; Urip; Iswari Pauzi; Thomas Tandi Manu
Journal of Indonesia Laboratory Students (JILTS) Vol. 2 No. 1 (2023): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v2i1.12

Abstract

Pemeriksaan laboratorium merupakan pemeriksaan yang menunjang keselamatan untuk menegakkan diagnosis suatu penyakit salah satunya pemeriksaan urinalisis. Rancangan penelitian ini merupakan penelitian observase analitik dengan pendekatan cross sectional pada sampel urine pagi dan urine sewaktu penderita gagal ginjal. Menunjukkan tidak ada perbedaan antara urine pagi dan urine sewaktu pada pemeriksaan kimiawi urine metode carik celup 10 parameter. Jenis spesimen urine pagi dan sewaktu tidak ditemukan perbedaan yang signifikan saat pemeriksaan kimiawi urine.
Co-Authors Agrijanti, Agrijanti Ananda, Dewa Ayu Putu Wiweka Aryati Aryati Baiq Eliya Yusrina Eliya Cahayaningrum, Aryani Dewi, Lale Budi Kusuma Eka Atyi Purwasih Erlin Yustin Tatontos Fihiruddin, Fihiruddin Getas, I Wayan Helenia Ananda Feriska Felanis Heny Arwati I Gusti Ayu Ambarawati Iswara Sesa I Made Suardika I Made Wahyu Waisnawa I Wayan Getas Iswari Pauzi Khusuma, Ari Kristinawati, Erna Lala Deswinta Yusari Lalu Srigede Lalu Srigede, Lalu Maruni Wiwin Diarti Muhammad Luthfi Nurul Inayati Pancawati Ariami Paulina Rosa Evriarti Pauzi, Iswari Puspa Wardhani Putri Sukma Diani Putri, Ananda Kinanti RAI WIADNYA, IDA BAGUS Reginata, Ni Luh Vira Rohmi Rohmi, Rohmi Salsabila, Yaumi Tasu’a Sari Artauli Lumban Toruan Sufina Zikria Putri Urip Urip Urip, Urip Waisnawa, I Made Wahyu Widiasih, Ni Kadek Yaumi Tasu’a Salsabila Yunan Jiwintarum Yusari, Lala Deswinta Zaena Safitri, Trysia