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<![CDATA[IDENTIFIKASI ALEL DAN KEKUATAN PEMBEDA EMPAT LOKUS DNA MIKROSATELIT KROMOSOM-Y PADA MASYARAKAT KLAN PANDE DI BALI UNTUK KEPENTINGAN FORENSIK ]]>
<![CDATA[I Ketut Junitha]]>;
<![CDATA[I Made Sara Wijana]]>
<![CDATA[ Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 7 (2017): Indonesian Journal of Legal and Forensic Sciences ]]>
Publisher : <![CDATA[Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana ]]>
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DOI: 10.24843/IJLFS.2017.v07.i01.p01
<![CDATA[This research was conducted to know alleles variation and power of discrimination of four pair of primer: DYS19, DYS390, DYS393 and DYS395 of male Pande clan. Epithelial cell mucus was collected from 59 male of Gainer, Klungkung, Badung regencies and Denpasar city. Phenol chloroform method was used to extraction DNA from epithel cells sample. Four primer pair was used to amplify DNA samples in PCR machine on 52-55oC annealing temperature. Amplicon were running on PAGE 10% and visualized with silver nitrate staining. The DNA typing were conducted to determinate of alleles size of amplicon with plotting migration distance of amplicon on semi-log pepper. Genetic diversity and power of discrimination was calculated used Microsoft Exell program. The result of this research showed that 29 alleles were found range of 6-9 with mean 7.25 per locus. The genetic diversity is high category (0.739 ± 0.003, the highest diversity on DYS 390 locus is 0.809 ± 0,004 followed by DYS395, DYS393 are 0,793 ± 0.004 and 0,720 ± 0.005 and the small one is 0.633 ± 0.003 on DYS19 locus. The power of discrimination (PD) of all loci are high category with average value is 0.892, because of / for this reason all of loci usfulnes for forensic purpose on pande clan in Bali.]]>
<![CDATA[Penanda DNA mikrosatelit Kromosom-Y untuk Penelusuran Soroh-soroh/Kawitan Masyarakat Bali ]]>
<![CDATA[I Ketut Junitha]]>
<![CDATA[ Journal of Tropical Ethnobiology 2021: Prosiding Seminar Nasional PMEI V 2020 ]]>
Publisher : <![CDATA[The Ethnobiological Society of Indonesia ]]>
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<![CDATA[Soroh is a Balinese term for a clan in Indonesian. Each soroh is a group of Balinese Hindus who believe they are descended from one common ancestor, and has one main temple as a place of worship to the ancestors, which is called the kawitan temple. Each main soroh can be divided into several sub-soroh based on the male lineage of the main ancestor. Each sub-soroh also has a temple for their ancestors’ worship which is also called pura kawitan. Therefore, there are known subsoroh and pura kawitan main temples, whose members are a combination of sub-soroh and sub-sections. Soroh Catur Sanak Bali Mula believes he is a descendant of Mpu Kamereka which developed into four sub-soroh, namely Kayu Selem, Celagi, Kayuan, and Terunyan. The family system in Bali adheres to a patrilineal descent where all children from one family will follow the cheer of their father. For Balinese Hindus, knowing their soroh or kawitan temple is an obligation to be able to offer prayers to their kawitan temple as a form of respect for each offspring to their ancestors. Losing traces of ancestors (kawitan) is believed to be a major problem in the family life. Based on the belief that each soroh is descended from one ancestor and the family system in Bali adheres to a patrilineal lineage, soroh or kawitan tracing can be carried out by DNA analysis with Ychromosome microsatellite markers. DNA or genes on the Y chromosome that are only present in males will be passed from males to only males and so on. Therefore, the Y-chromosome microsatellite profile can be used as a search tool for Soroh or Kawitan in Balinese society.]]>
<![CDATA[Hubungan Genetika Pria Masyarakat Sembiran dan Tri Wangsa di Bali ]]>
<![CDATA[I Ketut Junitha]]>;
<![CDATA[Bambang Suryobroto]]>
<![CDATA[ Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 1 (2010) ]]>
Publisher : <![CDATA[Fakultas Biologi | Universitas Jenderal Soedirman ]]>
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DOI: 10.20884/1.mib.2010.27.1.185
<![CDATA[Balinese community has evolved since the prehistoric era. The present community is structured into Hinduism caste (Brahmana, Kesatria, Wesia and Sudra), but remnants of their ancient communities, collectively called Bali Aga, have not undergone this structure. The first three castes of modern Balinese that comprises Tri Wangsa gentry were derived from JavanesekingdomofMajapahit(1343 AD). One of Bali Aga communities is Sembiran of Northern Bali. Using chromosomal microsatellite DNA, allelic variability of Sembiran and Tri Wangsa communities was reported. Both of them had the same high frequency of alleles which suggested genetic intermixing or sharing of common ancestor. However, a few alleles were unique for each community. This uniqueness was related to patrilineal system of Balinese community and to high mutation rate of Y chromosomal microsatellite DNA.]]>
<![CDATA[Laju dekomposisi bangkai mencit (Mus musculus) yang dikubur selama empat minggu pada media tanah humus, kapur, dan pasir pantai ]]>
<![CDATA[Cakra Diarsa]]>;
<![CDATA[I Ketut Junitha]]>;
<![CDATA[I Ketut Sundra]]>
<![CDATA[ Jurnal Biologi Udayana Vol 26 No 1 (2022): JURNAL BIOLOGI UDAYANA ]]>
Publisher : <![CDATA[Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana ]]>
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DOI: 10.24843/JBIOUNUD.2022.v26.i01.p08
<![CDATA[Dekomposisi bangkai terjadi segera setelah organisme mati mulai dari dekomposisi tingkat jaringan hingga tingkat molekuler. Laju dekomposisi bangkai hewan yang dikubur dipengaruhi oleh tiga faktor yaitu faktor fisik, kimia, dan biologi dari media yang digunakan mengubur bangkai. Faktor fisik berupa struktur, porositas, dan kelembaban dari media. Faktor kimia berupa pH, konsentrasi natrium, nutrien, dan oksigen yang terkandung pada media penguburan. Faktor biologi berupa jumlah dan komposisi dari koloni bakteri, invertebrata, dan flora yang hidup pada media. Tujuan dari penelitian ini adalah untuk mengetahui apakah terjadi perbedaan kecepatan dekomposisi bangkai pada media penguburan yang berbeda. Penelitian ini dilakukan dengan menggunakan bangkai mencit sebanyak 36 ekor yang dikuburkan pada tiga media berbeda yaitu tanah humus, pasir pantai, dan tanah kapur di dalam toples plastik. Masing-masing sebanyak 12 toples diisi dengan media yang sama sebagai ulangan. Bangkai mencit diamati selama 28 hari dimana tiap tujuh hari dilakukan penimbangan terhadap berat bangkai mencit dengan timbangan digital dan kondisi fisik dari bangkai mencit dicatat dan diskoring. Laju dekomposisi bangkai mencit ditunjukkan dari perbedaan rata-rata penurunan berat bangkai mencit (%) tiap minggu pada masing-masing media. Metode analisis data dilakukan dengan uji ANOVA. Hasil dari penelitian didapatkan adanya perbedaan penurunan berat dan nilai skor kondisi fisik bangkai mencit pada tiap media penguburan. Kesimpulan penelitian ini adalah waktu dan perbedaan media penguburan berpengaruh pada penurunan berat dan nilai skoring kondisi fisik bangkai mencit.]]>
<![CDATA[Kuantitas Dan Kualitas DNA Hasil Ekstraksi Dari Bercak Darah Pada Pisau Pasca Paparan Sinar Ultraviolet Dan Matahari ]]>
<![CDATA[Putri Arie Prasetyoningrum]]>;
<![CDATA[I Ketut Junitha]]>;
<![CDATA[Dwi Ariani Yulihastuti]]>
<![CDATA[ Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023) ]]>
Publisher : <![CDATA[Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana ]]>
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DOI: 10.24843/metamorfosa.2023.v10.i01.p19
<![CDATA[Examination of Deoxyribonucleic Acid is the primary identification method in forensic cases. Blood stains found at the scene of a crime are often affected by various environmental factors, one of which is ultraviolet light from the sun. Purpose of this study is to compare the quantity and quality of DNA extracted from blood stains on the blade after exposure to ultraviolet light and the sun for 0, 15 and 30 days. The method used is a factorial design: light source (UVA, indirect sunlight and direct sunlight); and duration of treatment (0, 15 and 30 days). Blood stains are made by dripping a 50?L of human blood on one side of the blade and then given treatment. DNA analysis includes: extraction with Chelex 5%; DNA quantity and quality test with SimpliNano spectrophotometer; total DNA quality test with agarose gel electrophoresis; and PCR. Quantitative data analysis using Univariate test followed by Duncan test. The results showed that the quantity of DNA in samples exposed to UVA and sunlight (directly) increased on the 15th day due to DNA fragmentation while samples exposed to sunlight (indirectly) decreased the quantity of DNA with the duration of treatment. DNA quality in the form of DNA purity produces an extract of DNA that is not pure. The total DNA quality with agarose gel electrophoresis on all samples showed that the longer the treatment (0, 15 and 30 days) resulted in a dimmer or thinner band of DNA luminescence with smears.]]>
<![CDATA[Optimasi digesti enzim restriksi untuk deteksi mutasi daerah D-loop DNA mitokondria dengan metode PCR-RFLP ]]>
<![CDATA[Ni Putu Senshi Septiasari]]>;
<![CDATA[I Ketut Junitha]]>;
<![CDATA[Ni Nyoman Wirasiti]]>
<![CDATA[ Jurnal Biologi Udayana Vol 27 No 1 (2023): JURNAL BIOLOGI UDAYANA ]]>
Publisher : <![CDATA[Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana ]]>
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DOI: 10.24843/JBIOUNUD.2023.v27.i01.p07
<![CDATA[Metode PCR-RFLP merupakan salah satu metode untuk deteksi mutasi pada daerah D-loop DNA mitokondria. Metode ini menggunakan enzim restriksi untuk dapat memotong DNA mitokondria dan menghasilkan ukuran fragmen DNA yang berbeda-beda. Enzim restriksi memerlukan kondisi yang optimal untuk melakukan pemotongan DNA. Penelitian ini bertujuan untuk mengetahui kondisi optimal enzim restriksi agar dapat melakukan digesti pada daerah D-loop DNA mitokondria. Optimasi dilakukan dengan membuat dua kombinasi formula digesti (formula 1 dan 2) dan empat macam waktu digesti (2 jam, 4 jam, 6 jam dan overnight). Hasil penelitian menunjukkan optimasi dari lima macam enzim restriksi (HaeIII (BsuRI), HindIII, HinfI, MboI dan HpyP31 (DdeI)) didapatkan bahwa ada perbedaan formula dan waktu digesti tergantung dari jenis enzim. Enzim HaeIII(BsuRI), HinfI dan MboI menunjukkan formula 2 merupakan formula optimal, sedangkan enzim HpyP31 (DdeI) formula 1 merupakan formula yang optimal. Waktu digesti 2 jam menunjukkan hasil optimal pada Enzim HaeIII(BsuRI), MboI dan HpyP31 (DdeI), sedangkan enzim HinfI waktu digesti optimal adalah 4 jam. Enzim HindIII tidak mendapatkan hasil potongan fragmen DNA setelah digesti, maka enzim HindIII tidak memiliki situs pemotongan pada daerah D-loop DNA mitokondria.]]>
<![CDATA[Ethnobotany of Medicinal Plant Diversity as A Traditional Medicine in Bugbug Karangasem, Bali, Indonesia ]]>
<![CDATA[Dewa Ayu Sri Ratnani]]>;
<![CDATA[I Ketut Junitha]]>
<![CDATA[ International Journal of Scientific Multidisciplinary Research Vol. 3 No. 1 (2025): January 2025 ]]>
Publisher : <![CDATA[PT FORMOSA CENDEKIA GLOBAL ]]>
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DOI: 10.55927/ijsmr.v3i1.12852
<![CDATA[Traditions and cultures used as tourism assets can ignore traditional practices with a concurrent loss of traditional knowledge. This study aims to reveal the traditional knowledge of medicinal plants used by the Bugbug community through analysis of the traditional knowledge of the diversity of medicinal plants, the fidelity level (FL) and Index of Cultural Significance (ICS) of medicinal plants. The study was conducted in Bugbug Village, Karangasem, from May to July 2022. Data were collected using qualitative methods through semi-structured interviews, moderate participation observation, and documentation. Key informants and respondents were selected using purposive and snowball sampling; 10 informants and 48 respondents were obtained. The fidelity level was calculated by FL; and cultural importance by ICS. The level of traditional knowledge of medicinal plants was measured by the Phillips and Gentry equations and analyzed by the Kruskal Wallis and Mann Whitney Test. As many as 124 species in 46 families are found at Bugbug Village. The best represented families were Fabaceae. Leaves are the most widely used plant parts for traditional medicine. The highest habitat is obtained from the roadsides. In terms of the life form, most of the used species were herbaceous. Traditional knowledge between age groups differs significantly. In contrast, traditional knowledge of gender was not found to be different. The highest fidelity levels are Piper betle L. and Imperata cylindrica L. And the most ICS is Piper betle L]]>
<![CDATA[Ethnobotany of Local Foodstuff Plants Used By Bugbug Community In Karangasem, Bali, Indonesia ]]>
<![CDATA[Dewa Ayu Sri Ratnani]]>;
<![CDATA[I Ketut Junitha]]>;
<![CDATA[Eswaryanthi Kusuma Yuni]]>;
<![CDATA[Desak Nyoman Budiningsih]]>
<![CDATA[ International Journal of Applied Science and Sustainable Development (IJASSD) Vol. 6 No. 1 (2024): International Journal of Applied Science and Sustainable Development (IJASSD) ]]>
Publisher : <![CDATA[Lembaga Penelitian dan `Pengabdian Kepada Masyarakat (LPPM) ]]>
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DOI: 10.36733/ijassd.v6i1.9124
<![CDATA[The aims of this research were (1) Reveal to the diversity of local foodstuff plants utilized by Bugbug community; (2) to analyze traditional knowledge of local foodstuffs plants of Bugbug community; (3) to analyze the Use Value (UV) and Index Cultural Significance (ICS) of local foodstuffs plants. The research was conducted in Bugbug Karangasem, Bali, from January 2021-March 2021. Data were collected using qualitative methods, semi-structured interviews, moderate participation observation, and documentation. Key informants were selected using purposive and snowball sampling to obtain ten key informants and 48 respondents. The use value of plants is calculated by UV, cultural importance with ICS. The level of traditional knowledge was measured by the Phillips and Gentry equations and analyzed by the Kruskal Wallis and Mann Whitney Test. The traditional knowledge of local foodstuff plants was calculated using the Phillips and Gentry equations and analyzed with the Kruskal Wallis and Mann Whitney Test. Traditional knowledge of local foodstuff plants between age groups is different. Meanwhile, knowledge between genders is not different. The diversity of local foodstuff plants in Bugbug Village is 126 species, 47 families, the most families being Musaceae. The high diversity of plants found is caused by specific ecosystems from hilly areas and beaches. The most widely used part of the plant is the fruit. Most plant habitus is an herb. Herbs are easy to grow in various locations and are found in semi-wild status. The highest plant use value and ICS are Arenga pinnata L.]]>
<![CDATA[Jenis-Jenis Diatom di Sungai Asahan dan Bak Mandi Sekitar Kabupaten Toba ]]>
<![CDATA[Putri Karenina Siregar]]>;
<![CDATA[I Ketut Junitha]]>;
<![CDATA[Ni Made Suartini]]>
<![CDATA[ Metamorfosa: Journal of Biological Sciences Vol. 11 No. 2 (2024) ]]>
Publisher : <![CDATA[Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana ]]>
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DOI: 10.24843/metamorfosa.2024.v11.i02.p6
<![CDATA[Diatom adalah organisme uniseluler, mikroskopis, serta tersusun dari dinding silika yang tahan terhadap pemanasan dan pengasaman. Diatom dapat ditemukan di berbagai jenis perairan. Penelitian ini bertujuan untuk mengetahui persamaan dan perbedaan jenis diatom yang ditemukan di Sungai Asahan dan bak mandi sekitar Kabupaten Toba. Sampel air diambil sebanyak 50 liter di tiap lokasi kemudian disaring menggunakan plankton net dan ditampung dalam botol kaca 25 ml. Sampel dalam botol diberi 10 tetes larutan iodin sebagai pengawet. Identifikasi diatom dilakukan dengan mengamati karakter morfologi dan morfometri menggunakan Image Raster III. Analisis data dilakukan secara deskriptif komparatif dan mengacu pada pustaka untuk menentukan jenis diatom. Hasil penelitian ini memperoleh sebanyak 40 jenis diatom dari 21 genus, 13 famili, sembilan ordo, dan satu kelas yang sama Bacillariophyceae. Sebanyak 37 jenis diatom ditemukan dari sampel air Sungai Asahan. Hanya empat jenis diatom yang ditemukan di wadah air mandi I. Demikian pula, hanya dua jenis diatom yang ditemukan di wadah air mandi III. Namun, lebih banyak jenis diatom yang ditemukan di wadah air mandi II, yaitu 14 jenis. Empat belas jenis diatom ditemukan baik di Sungai Asahan maupun di bak mandi. Jumlah sel diatom yang paling ditemukan adalah jenis Aulacoseira granulata. Kata kunci: bak mandi, diatom, sungai]]>
<![CDATA[Jenis-Jenis Diatom di Sungai Asahan dan Bak Mandi Sekitar Kabupaten Toba ]]>
<![CDATA[Putri Karenina Siregar]]>;
<![CDATA[I Ketut Junitha]]>;
<![CDATA[Ni Made Suartini]]>
<![CDATA[ Metamorfosa: Journal of Biological Sciences Vol. 11 No. 2 (2024) ]]>
Publisher : <![CDATA[Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana ]]>
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DOI: 10.24843/metamorfosa.2024.v11.i02.p6
<![CDATA[Diatom adalah organisme uniseluler, mikroskopis, serta tersusun dari dinding silika yang tahan terhadap pemanasan dan pengasaman. Diatom dapat ditemukan di berbagai jenis perairan. Penelitian ini bertujuan untuk mengetahui persamaan dan perbedaan jenis diatom yang ditemukan di Sungai Asahan dan bak mandi sekitar Kabupaten Toba. Sampel air diambil sebanyak 50 liter di tiap lokasi kemudian disaring menggunakan plankton net dan ditampung dalam botol kaca 25 ml. Sampel dalam botol diberi 10 tetes larutan iodin sebagai pengawet. Identifikasi diatom dilakukan dengan mengamati karakter morfologi dan morfometri menggunakan Image Raster III. Analisis data dilakukan secara deskriptif komparatif dan mengacu pada pustaka untuk menentukan jenis diatom. Hasil penelitian ini memperoleh sebanyak 40 jenis diatom dari 21 genus, 13 famili, sembilan ordo, dan satu kelas yang sama Bacillariophyceae. Sebanyak 37 jenis diatom ditemukan dari sampel air Sungai Asahan. Hanya empat jenis diatom yang ditemukan di wadah air mandi I. Demikian pula, hanya dua jenis diatom yang ditemukan di wadah air mandi III. Namun, lebih banyak jenis diatom yang ditemukan di wadah air mandi II, yaitu 14 jenis. Empat belas jenis diatom ditemukan baik di Sungai Asahan maupun di bak mandi. Jumlah sel diatom yang paling ditemukan adalah jenis Aulacoseira granulata. Kata kunci: bak mandi, diatom, sungai]]>
