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All Journal HAYATI Journal of Biosciences INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES METAMORFOSA Journal of Biological Sciences Jurnal Farmasi Udayana INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Biologi Udayana Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology SIMBIOSIS Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Kajian Bali Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Journal of Tropical Ethnobiology International Journal of Applied Science and Sustainable Development (IJASSD) International Journal of Scientific Multidisciplinary Research (IJSMR)

I Ketut Junitha

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 1918405

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mechanical Engineering Medicine & Pharmacology Social Sciences

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IDENTIFIKASI ALEL DAN KEKUATAN PEMBEDA EMPAT LOKUS DNA MIKROSATELIT KROMOSOM-Y PADA MASYARAKAT KLAN PANDE DI BALI UNTUK KEPENTINGAN FORENSIK I Ketut Junitha; I Made Sara Wijana
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 7 (2017): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2017.v07.i01.p01

This research was conducted to know alleles variation and power of discrimination of four pair of primer: DYS19, DYS390, DYS393 and DYS395 of male Pande clan. Epithelial cell mucus was collected from 59 male of Gainer, Klungkung, Badung regencies and Denpasar city. Phenol chloroform method was used to extraction DNA from epithel cells sample. Four primer pair was used to amplify DNA samples in PCR machine on 52-55oC annealing temperature. Amplicon were running on PAGE 10% and visualized with silver nitrate staining. The DNA typing were conducted to determinate of alleles size of amplicon with plotting migration distance of amplicon on semi-log pepper. Genetic diversity and power of discrimination was calculated used Microsoft Exell program. The result of this research showed that 29 alleles were found range of 6-9 with mean 7.25 per locus. The genetic diversity is high category (0.739 ± 0.003, the highest diversity on DYS 390 locus is 0.809 ± 0,004 followed by DYS395, DYS393 are 0,793 ± 0.004 and 0,720 ± 0.005 and the small one is 0.633 ± 0.003 on DYS19 locus. The power of discrimination (PD) of all loci are high category with average value is 0.892, because of / for this reason all of loci usfulnes for forensic purpose on pande clan in Bali.

ANALISA KERAGAMAN GENETIK KELAPA RANGDA (Cocos nucifera L.) DI BALI BERDASARKAN PENANDA DNA MIKROSATELIT Ni Luh Putu Rika Sugiantari; I Ketut Junitha; Eniek Kriswiyanti
SIMBIOSIS Vol 3 No 2 (2015)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.586 KB)

The research aimed to analyze genetic variation of Rangda coconut in Bali based on microsatellite DNA markers. The young leaf (busung) were collected from Gunaksa village at Klungkung regency, Tulikup village at Gianyar regency, Kubu village at Bangli regency, Sanghyang village, Jembrana regency one sample each, and three samples are from Ngis village at Karangasem regency. Doyle and Doyle DNA extraction method was used to extract DNA from the samples. Three primer pairs of CnCirC3, CNZ05 and CNZ51 were used to amplify DNA samples. Amplicons were electrophoresed on PAGE 10% and stained with silver nitrate. The allele size was determined by plotting the amplicon migration distance on semilog paper. The results showed there were 11 alleles with an average of 13.33 alleles per primer. There were 3 alleles on primer CNZ05, 4 alleles on primer CnCirC3 and CNZ51 each. Heterozigosity expectation (He) value on primer CnCirC3, CNZ05, and CNZ51 were 0,49±0,04, 0,27±0,03 and 0,71±0,06 consecutively. The result showed that Genetic relationship among 7 coconut samples were divided into two big groups. Key word : Rangda coconut, Microsatellite marker, Number allele, Genetic relationship

PENGARUH PENCUCIAN DETERJEN HASIL EKSTRASI DNA SPERMATOZOA PADA KAIN KATUN Christian Subagya Gunardi; I Ketut Junitha; Inna Narayani
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2021.v09.i02.p01

The presence of spermatozoa DNA is reliably counted as a solid evidence when dealing with rape cases. However, police department often finds difficulties to obtain it, mainly because it has been destroyed by the perpetrator. Such act can be done by washing semen stains on the clothing with detergent. The purpose of this research is to analyze the impact of detergent as a washing property towards spermatozoa DNA extract on a cotton fabric. Observed variables in this study are quantity and quality of spermatozoa DNA extract from semen stain on cotton fabric. Samples are divided based on two separate treatments; detergent-washed and unwashed. Furthermore, samples are stored with different storage time. This research applied two methods; DNA extraction using kit (Roche) and DNA quantification using spectrophotometer Nanodrop. The research was held at Biomedic Laboratory, Faculty of Medicine, Udayana University, Denpasar with ethical clearance. Seminal plasma sampel was obtained from a donor with informed consent. This research showed that seminal stain washing on cotton fabric doesn't remove the DNA until two-week-storage time.

PROFIL STRUKTUR SERAT IBU TANGKAI DAUN ANTARA INDUK DAN ANAKAN KELAPA (Cocos nucifera L “Rangda”) Ni Made Puspawati; Eniek Kriswiyanti; I Ketut Junitha
SIMBIOSIS Vol I, No 2, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.905 KB)

The research aims to determine the anatomical structure of the leaf stalk fiber Coconut(Cocos nucifera L. "Rangda") on the seedling and parent plant. Sampling was conducted atSanghyang village Melaya district, Regency of Jembrana. Preparations and microscopicobservations made in the laboratory of Plant Development Structure Biology Department,FMIPA Unud. The research was conducted in October 2012 - April 2013. Maceration methodused for preparations sklerenkim fibers and xylem elements. The results showed that theanatomical parent plant and seedling petiole were torque and mostly composed bysclerenchym fibers but in the parent plant more torque than seedling. In the parent plant about81% the sclerenchym cell was torque but in seedling only 13%, with an average number of17 ± 1 torsion of the parent plant and 6 ± 1 in seedling.Keywords: the fiber structure, torque

TINGGI DAN BERAT BADAN SISWA UMUR 10-12 TAHUN DI SEKOLAH PEDESAAN DAN KOTA : SD DI DESA PINANG SEBATANG TIMUR DAN KOTA PEKANBARU PROVINSI RIAU Fita Efi Rafelina Siallagan; I Ketut Junitha; Iriani Setyawati
SIMBIOSIS Vol 10 No 1 (2022)
Publisher : Jurusan Biologi Universitas Udayana

ABSTRACT The difference in height and weight is influenced by genetic traits and environmental factors. The study population was primary school children from one elementary school in the area of Pinang Sebatang Timur Village, Tualang Regency and one elementary school in urban areas covering Pekanbaru City. The two research areas are located in the province of Riau. The research sample was 60 students from Nusantara elementary school in Pinang Sebatang Timur Village and 60 students from Santa Maria elementary school, which consisted of (30 boys and 30 girls). The age range of the probandous is about 10-12 years old. The method that is being used for the research is purposive sampling method using online survey. The parameters studied were height, weight, age, parental education, parental occupation, parental income, body mass index (BMI), age of manarche and first wet dreams. Body Mass Index (BMI) was calculated by dividing body weight (kg) by height square (m2).According to the Center for Disease Control and Prevention, BMI is categorized based on 4 categories, namely underweight (<18), normal (18-25), overweight (25-27), and obese (>27). The average of heights, weights and body mass indexes of male and female students in Pekanbaru city are higher than in the Pinang Sebatang Timur’s village. Factors that are affecting student’s heights and weights are their parent’s job, their parent’s allowances, heights, the age of menarche on the female children or wet dream on the male children, and also the physical activities the students do. Keywords: Growing pattern, height, weight, age, Body Mass Index (BMI)

FREKUENSI GEN CUPING MELEKAT, ALIS MENYAMBUNG, LESUNG PIPI DAN LIDAH MENGGULUNG PADA MASYARAKAT DESA SUBAYA, KECAMATAN KINTAMANI, KABUPATEN BANGLI Yulia Mirayanti; I Ketut Junitha; Ida Bagus Made Suaskara
SIMBIOSIS Vol 5 No 1 (2017)
Publisher : Jurusan Biologi Universitas Udayana

This study was conducted to find out the determinant allele frequencies and variations of characteristics of face and earlobe, based on the presence or absence of attached earlobes, connected eyebrows (unibrow), dimples, and tongue-rolling on Subaya village people, Kintamani, Bangli. In this study, 100 samples of proband (67 men and 33 women) as the research data were tested by using Chi-Square Test. Then, the data were analyzed by pedigree diagram to each family. The results of study show that 19% of people have the characteristic of attached earlobes, 12% of people have the characteristic of connected eyebrows, 7% of people have the characteristic of dimples, and 16% of people have the characteristic of tongue-rolling. The highest gene frequency value is the characteristic of attached earlobes (0,43), then, followed by the characteristics of connected eyebrows (0,34) and tongue-rolling (0,084) as well as the lowest gene frequency is the characteristic of dimples (0,036). Keywords: autosomal inheritance, genetic variation, Subaya village, inbreeding

VARIASI SPESIES DIATOM PADA TIPE PERAIRAN BERBEDA UNTUK KEPENTINGAN FORENSIK SEBAGAI PETUNJUK KEMATIAN AKIBAT TENGGELAM Adelina Arifiani Purnomo; I Ketut Junitha; Ni Made Suartini
SIMBIOSIS Vol 3 No 1 (2015)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.417 KB)

The purpose of this research was to determine diatom species in Unda River, BadungRiver, Lake Beratan, estuarine and marine waters of Padang Galak, that can be used to indicatedeath scene caused by drowning. Waters collected to 50 liters by using plankton net and carriedin a 25 ml glass bottle. The results of the research found that there were 60 species diatom from26 genera, 23 families, 2 orders, and 1 class. Among the 60 species in this research, can beindicate as 12 species are typical species of Unda River, 5 species are typical species of BadungRiver, 1 species is typical of Lake Beratan, 9 species are typical of Padang Galak Estuary, and 19species are typical to Padang Galak Sea. Among the typical species in every research site, thereare one species that has higher number: Fragilaria sp. 1 in Unda River, Gomphonema sp. 3 inBadung River. Cymbella sp. 3 in Lake Beratan, Cyclotella sp., in Padang Galak Estuarine, andCocconeis sp. 2 in Padang Galak Sea.Keywords: forensic, diatom, river, lake, sea

DNA HASIL EKSTRAKSI DARI BERCAK SPERMA PADA KAIN KATUN DAN POLIESTER YANG DISIMPAN HINGGA 40 HARI I Gede Yeyen Suharta; I Ketut Junitha; A.A.S.A. Sukmaningsih
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

DNA is an important evidence in forensic-based investigation which is useful in the process of solving criminal cases. Rape is an act of crime which could leave evidence such as sperm stain on fabric. The said stain can be processed to obtain the perpetrator’s DNA and uncover their identity. This study aims to determine whether DNA from sperm stains on cotton and polyester fabrics which had been stored for 0, 20, and 40 days could be extracted and to determine the quality and quantity of the extracted DNA. This study conducted from September to December 2020 at the Serology and Molecular Forensics of UPT Laboratory and the Integrated Biomedical Laboratory of Udayana University. The research method consisted of sample preparation, DNA extraction using Chelex 100 resin, and DNA quantification and qualification using NanoDrop. Data from DNA quantity are analyzed using two-way ANOVA and chi-squared test method. Results of this study shows that DNA can be extracted from sperm stains on cotton and polyester fabrics which are stored for up to 40 days. The decrease of DNA quality and quantity in all samples directly proportional to the increase of the storage time treatment. The decrease of DNA quantity on cotton fabric occurs by 80.42% (fresh-0 day), 12.02% (0 day-20 days), and 0.78% (20 days-40 days). Decrease of DNA quantity on polyester fabric occurs by 62.16% (fresh-0 day), 11.40% (0 day-20 days), and 8.47% (20 days-40 days). Statistical analysis on the quantity of DNA showed that the rate of reduction in DNA concentration was influenced by the type of cloth used.

VARIASI POLA SIDIK JARI SOROH BRAHMANA SIWA DI BALI Ida Bagus Bajing Agastya; I Ketut Junitha; Ni Nyoman Wirasiti
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

ABSTACTResearch was conducted to determine the patterns of Brahmana Shiva fingerprint in Bali. The fingerprint samplesas many as 240 people, which consist of 120 men and 120 women from four generation of Brahmana Shiva in Bali. Thefingerprints were collected using a fingerprint pad then the data were analyzed using Chi–Square test, Student-t test,Dankmerijer index and Furuhata index. The result of fingerprints examination shown that there were four fingerprintspattern i.e. arch (A), loop ulnar (LU), loop radial (LR), and whorl (W) from four sub clan (Brahmana Manuaba, Kemenuh,Keniten, Mas), with different frequencies and anequal distribution from each fingers and both hands. The arch (A) patternin clan Brahmana Manuaba and Kemenuh have the same 0.83 % frequencies, while the frequencies of Keniten 1.17 % andMas 1.67 %. The frequencies of Loop ulnar (LU) pattern from clan Brahmana Mas were 42.66 %, Manuaba 36 %, Keniten39 %, and Kemenuh 34.33 %. The frequency of loop radial (LR) pattern from clan Brahmana Manuaba were 42.17 %,Keniten 41.83 %, Mas 37.83 %, and Kemenuh 29.51 %. The frequencies of whorl (W) pattern from sub clan BrahmanaKemenuh, Manuaba, Keniten, and Mas were 35.33 %, 22 %, 18 %, and 17.83 %, respectively. The result show asignificant differences between sub clan Mas and Kemenuh using Dankmerijer Index (DI). The result show a significantdifferences between sub clan Kemenuh with Mas, Keniten and Manuaba using Furuhata Index (FI). Total Ridge Countbetween men and women of Brahmana Shiva offspring showed unsignificantly diferences at level 5%. However, there wasa significant difference of Total Ridge Count from sub clan Manuaba with Mas, Kemenuh, and Keniten.Keywords : fingerprints, arch, loop, whorl, clan brahmana shiva.

EKSTRAKSI DNA DARI SIKAT GIGI BERDASARKAN LAMA PEMAKAIAN DAN LAMA PENYIMPANAN SETELAH DIPAKAI Agriani Dewinta; I Ketut Junitha; Made Pharmawati
SIMBIOSIS Vol 10 No 1 (2022)
Publisher : Jurusan Biologi Universitas Udayana

In recent years in Indonesia there have been frequent disasters, including natural disasters, non-natural disasters, and social disasters that have resulted in many casualties. The identification process of victims who have experienced natural disasters, non-natural disasters, and social disasters often uses DNA. The primary identification method commonly used in DVI is DNA. Disaster Victim Identification (DVI) is a procedure for identifying victims who died from a mass disaster that can be scientifically accounted for and refers to the Interpol standard. Sources of DNA data consist of primary and secondary data sources. Toothbrush is a secondary source of DNA data. Toothbrush is commonly used as a source of comparative DNA samples selected in cases of identification of dead victims without a family as a comparison. In this study, it was shown that the time of use and the part of the toothbrush had no significant effect (P> 0.01) on the amount of DNA. The results of the quality test using gel electrophoresis were only 7 samples, namely 2b, 3a, 10a, 10b, 11a, 11b, 12b which showed that there was a thin band of luminescence and DNA smears. PCR-RAPD test was performed to test the extracted DNA could be amplified. PCR-RAPD results from 12 DNA samples, 7 samples were successfully amplified, indicating that the DNA extracted from a toothbrush could be amplified.

Co-Authors A.A. Gde Lanang Adelina Arifiani Purnomo Agriani Dewinta Anastesya Hartika Nur Saputri Ayu Saka Laksmita W Bagas Susilo Bagus Alit, Ida Bambang Suryobroto Bram Setyadji Budiningsih, Desak Nyoman Cakra Diarsa Chandra Yowani Christian Subagya Gunardi Desak Nyoman Budiningsih Dewa Ayu Sri Ratnani Dewa Ayu Sri Ratnani Dwi Ariani Yulihastuti ENIEK KRISWIYANTI Eswaryanthi Kusuma Yuni Fita Efi Rafelina Siallagan Graciella Stevani Gulo Gusti Ayu Putri Arnila Hanas, Dicky Frengky I Gde Suryadi I Gede Yeyen Suharta I Gusti Agung Eka Saraswati I K. Sukardika I Ketut Sundra I Made Sara Wijana I NYOMAN MANTIK ASTAWA I Wayan Arya Mahardika Ida Ayu Manik Damayanti Ida Bagus Alit Ida Bagus Bajing Agastya Ida Bagus Made Suaskara IMA Gelgel Wirasutha Inna Narayani IRIANI SETYAWATI Kezia Artanauli Purba Lucia Emy Octavia MADE PHARMAWATI Maya Agustina Maya Agustina Meitini Proborini Wahyuni Muhamad Masyrur NI KADEK YUNITA SARI Ni Luh Putu Ria Puspitha Ni Luh Putu Rika Sugiantari Ni Luh Watiniasih Ni Made Puspawati Ni Made Rai Suarni Ni Made Suartini Ni Nyoman Wirasiti Ni Putu Puniari Eka Putri, Ni Putu Puniari Eka Ni Putu Putri Wulandari Putri Arie Prasetyoningrum Putu Rosi Setyari SANG KETUT SUDIRGA Septiasari, Ni Putu Senshi Tyara Tyara Victor Peter Poli Yan Ramona Yossy Carolina Carolina YOSSY CAROLINA UNADI Yulia Mirayanti

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