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All Journal HAYATI Journal of Biosciences INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES METAMORFOSA Journal of Biological Sciences Jurnal Farmasi Udayana INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Biologi Udayana Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Kajian Bali Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Journal of Tropical Ethnobiology International Journal of Applied Science and Sustainable Development (IJASSD) International Journal of Scientific Multidisciplinary Research (IJSMR) Metamorfosa: Journal of Biological Science Open Access DRIVERset

I Ketut Junitha

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 1918405

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mechanical Engineering Medicine & Pharmacology Social Sciences

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Variasi genetik dan jarak genetik suku Sakai di Provinsi Riau dengan suku Minangkabau di Desa Pagaruyung Sumatera Barat berdasarkan DNA mikrosatelit Tyara Tyara; I Ketut Junitha; Ni Nyoman Wirasiti
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p03

There are several versions can explain the origin of the Sakai tribe. Sakai people themselves believe that their ancestors came from Pagaruyung, West Sumatra Province. The purpose of this research is to determine the genetic variation and genetic distance of the Sakai tribe in Riau Province and the Minangkabau tribe in Pagaruyung Village, West Sumatra Province based on three microsatellite DNA loci (D2S1338, D16S539, and D13S317). A total of 10 alleles were found in three microsatellite loci of the Sakai tribe and 11 alleles in the Minangkabau tribe. Data analysis using GenAlex 6.503 software showed that the heterozygous value of the Sakai and Minangkabau tribes was moderate with a value of 0.557 ± 0.062 in the Sakai tribe and 0.615 ± 0.073 in the Minangkabau tribe. Meanwhile, the value of genetic differentiation (F’ST) in these two tribes shows moderate genetic differences (0.088 ± 0.039). Genetically, Sakai people and Minangkabau tribes have similarities based on the D2S1338, D16S539, and D13S317 microsatellite DNA loci with a genetic distance of 0.269 and the proportion of identical genes (Genetic identity) of 0.764. This result is supported by alleles outflow or entry in the Sakai and Minangkabau tribes with a migration rate is four people per generation.

VARIASI GENETIK SOROH BRAHMANA BUDHA DI BALI BERDASARKAN PENANDA DNA MIKROSATELIT KROMOSOM-Y Ida Ayu Manik Damayanti; I Ketut Junitha; Ida Bagus Made Suaskara
Jurnal Biologi Udayana Vol 18 No 2 (2014): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (736.611 KB)

Perbedaan sifat-sifat indukatau orang tua dalam ilmu genetika akan diturunkan secara turun-temurun kepada keturunannya sehingga terdapat variasi genetik antar individu. Variasi genetik dapat dilihat dari ragam alel pada masing-masing lokus mikrosatelit kromosom Y (Y-spesifik). Penelitian ini bertujuan untuk mengetahui variasi genetik soroh Brahmana Budha di Bali. Pada penelitian ini digunakan empat lokus DNA mikrosatelit kromosom Y yaitu DYS19, DYS390, DYS393 dan DYS395. Amplifikasi DNA dilakukan dengan menggunakan reagen PCR master mix i-Taq dengan mesin PCR dan amplikon dielektroforesis pada gel poliakrilamid 6% serta pewarnaan dengan perak nitrat. Hasil penelitian ini mendapatkan bahwa tidak semua Kabupaten di Bali terdapat Griya pendeta Budha. Ditemukan sembilan ragam alel yaitu masing-masing dua pada lokus DYS19 (200 dan 204 pb), DYS390 (203 dan 207 pb), dan DYS393 (125 dan 137 pb) dan lokus DYS395 ditemukan tiga ragam alel (123,127 131 pb). Padapenelitian ini ditemukan tiga haplotipe yaitu haplotipe A,Bdan C. Haplotipe A memiliki frekuensi paling tinggi (0,85) yang tersebar di lima daerah, haplotipe B ditemukan di daerah Karangasem sedangkan haplotipe C hanya ditemukan di Kabupaten Tabanan. Kombinasi alel pada haplotipe C yang sangat berbeda dengan haplotipe A dan B, menunjukkan bahwa individu dengan haplotipe C bukan keturunan soroh Brahmana Budha. Nilai keragaman rata-rata atau heterozigositas pada soroh Brahmana Budha di Bali rendah yaitu 0,15 ± 0,07.

Keragaman genetik masyarakat Pande Bangke Mawong di Desa Sumita, Kabupaten Gianyar, Bali berdasarkan penanda DNA mikrosatelit kromosom Y I Gde Suryadi; I Ketut Junitha; Made Pharmawati
Jurnal Biologi Udayana Vol 24 No 1 (2020): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Masyarakat Pande Bangke Mawong di Desa Sumita merupakan salah satu bagian klan besar Pande di Bali. Asal-usul masyarakat Pande di Desa Sumita tersebut diketahui masih menjadi kontroversi. Penelitian ini dilakukan untuk mengetahui keragaman genetik masyarakat Pande Bangke Mawong di Desa Sumita berdasarkan penanda DNA mikrosatelit kromosom Y. Sampel diambil di Desa Sumita dan beberapa desa di Bali sebagai pembanding dengan metode purposive sampling. Tahapan penelitian meliputi ekstraksi DNA dengan metode fenol-kloroform yang telah dimodifikasi, amplifikasi DNA menggunakan penanda DNA mikrosatelit dengan lima macam primer yaitu DYS19, DYS390, DYS393, DYS395, dan SRY, elektroforesis produk PCR dengan Polyacrilamide Gel Electrophoresis (PAGE) 10%, visualisasi DNA dengan metode pewarnaan perak nitrat, dan DNA typing ditentukan melalui ukuran panjang DNA amplikon dengan mengukur jarak migrasinya pada gel yang diplot pada kertas semilog. Data frekuensi alel dan keragaman genetik dianalisis menggunakan software GenAlex 6.5. Hubungan kekerabatan dianalisis dengan metode UPGMA menggunakan software MEGA 7. Ditemukan delapan alel dan tiga haplotipe pada masyarakat Pande Bangke Mawong di Desa Sumita. Pada masyarakat Pande lainnya ditemukan 12 alel dan enam haplotipe. Nilai keragaman genetik masyarakat Pande di Desa Sumita adalah 0,035±0,014, sedangkan pada masyarakat Pande lainnya sebesar 0,432±0,112. Ragam alel dan haplotipe yang ditemukan pada masyarakat Pande Bangke Mawong menunjukkan adanya proses mikroevolusi yang disebabkan mutasi genetik. Hubungan kekerabatan masyarakat Pande di Desa Sumita paling dekat dengan masyarakat Pande di Desa Tenganan Pegringsingan dan Desa Renon yang dikenal sebagai salah satu desa tua di Bali.

SUCCESSFUL GENETIC CHARACTERIZATION OF BULLET TUNA (Auxis rochei) USING MICROSATELLITE MARKERS IN KARANGASEM BALI Maya Agustina; Made Pharmawati; Ketut Junitha
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 17, No 3 (2021): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.17.3.%p

Bullet Tuna (Auxis rochei) is classified under the neritic tuna group, which plays an essential role in small-scale fisheries in Karangasem Bali. The increasing catch of bullet tuna indicated its stock probably under threat. Therefore, genetic characterization is often required as the first step before building a conservation program. This study aims to categorize bullet tuna DNA using microsatellite. Of all five loci used, all were high polymorphism-type, with the number of alleles per locus varied between 18-27. Successful PCR (Polymerase Chain Reaction) created relatively high DNA concentration, ranging from 27.050 to 237.05 ng/ul, with a DNA purity level ranging from 2.073 to 2.239. Overall, the stock condition allegedly still in good condition, marked with high genes diversity (Ho=0.367-0.767 and He=0.934-0.966). All loci used can be amplified and well described.

Genetic Diversity and Population Structure of Bullet Tuna (Auxis rochei) from Bali and Its Adjacent Waters Maya Agustina; Bram Setyadji; Made Pharmawati; I ketut Junitha
HAYATI Journal of Biosciences Vol. 29 No. 4 (2022): July 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.4.507-514

Bullet tuna (Auxis rochei) dominates the neritic tuna catch, especially from the purse seine fleet within the western and southern Indonesian waters. However, high catches can lead to stock depletion and lower genetic diversity due to possible inbreeding. Therefore, population genetic information is important in monitoring the sustainability of fish stocks and proposing an appropriate species-specific conservation strategy. This study aimed to analyze the genetic diversity, population structure, and kinship relationship of bullet tuna in Bali and its adjacent waters. Sampling was carried out in September 2020 at landing sites/ports representing the north, east, south, and west region, whereas at least 30 samples were acquired at each location. The result showed that the DNA concentration obtained could produce DNA bands with allele length ranged from 94-260 bp. Observed heterozygosity (Ho) was around 0.440-0.627. While the expected heterozygosity (He) was between 0.932-0.945. The genetic variation among population, within-population, and individuals was 0.36%, 41.04%, and 58.60%, respectively. The results of the analysis of genetic diversity between individuals in the population showed very high genetic diversity. The population structure of the bullet tuna landed in West Bali, East Bali, South Bali and North Bali is the same population stock. The kinship relationship indicates that the four populations are closely related genetically.

Ragam Alel Mikrosatelit DNA Autosom pada Masyarakat Bali Aga Desa Sembiran Kabupaten Buleleng Bali I Ketut Junitha; Ida Bagus Alit
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 1 (2011): February 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i1.60

Berdasarkan data sejarah dan arkeologis, masyarakat Bali sekarang ini merupakan hasil perkembangan sejarah zaman pra-sejarah. Masyarakat Bali kuno yang masih memiliki tradisi zaman pra-sejarah disebut masyarakat Bali Aga atau Bali Mula. Pada umumnya masyarakat Bali Aga menempati daerah pegunungan seperti desa Tenganan, Terunyan, Sembiran dan Sidatapa, sedangkan masarakat Bali lainnya disebut masyarakat bali Dataran yan tinggal di kota-kota dan daerah pantai. Sebanyak delapan penanda genetik mikrosatelit autosom (D2S1338, D3S1358, D5S818, D7S820, D11S1984, D13S317, D16S539 dan D21S11) digunakan untuk menentukan variasi alel yang tersebar pada masyarakat desa Bali Aga Sembiran kabupaten Buleleng Bali untuk kepentingan forensik. Dalam penelitian ini ditemukan sebanyak 46 ragam alel dari 8 lokus yang digunakan, ragam alel perlokus berkisar antara 3 pada lokus D5S818 sampai 9 alel pada lokus D11S1984. Nilai kemampuan pembeda (power of discrimination/PD) tertinggi ditemukan pada lokus D11S1984 (0,9394) diikuti oleh D21S11(0,8922), D16S539 (0,8915), D13S317 (0,8602), D7S820 (0,8398), D3S1358 (0,8014), D2S1338 (0,5518) dan D5S818 (0,0143). Hasil ini menunjukkan bahwa lokus D5S818 tidak baik digunakan dalam analisis DNA untuk kepentingan forensik pada masyarakat Bali Aga desa Sembiran.

Profil genetika DNA mikrosatelit kromosom-Y masyarakat laki-laki soroh Kayuan Pasek Catur Sanak Bali Mula I Ketut Junitha; Ni Luh Watiniasih; Ni Luh Putu Ria Puspitha
Jurnal Kajian Bali (Journal of Bali Studies) Vol 7 No 1 (2017): RELASI ETNISITAS DI BALI
Publisher : Pusat Kajian Bali Universitas Udayana

This research is conducted to find out the genetic profiles of Kayuan clan which is belong to Pasek Catur Sanak Bali Mula, indigenous Balinese people. The DNA profiles is identified by using Microsatellite DNA of Y chromosome. Genes or DNA in Y chromosome are descended from father to son (patrilinealism). Four loci of microsatellite DNA from Y chromosome are used, including DYS19, DYS390, DYS393 and DYS395 to amplified DNA samples from 67 males probands. The probands are people of Kayuan clan from Siakin, Songan, Blandingan and Kutuh villages, Kintamani sub district and others are originally from Jehem village, Tembuku sub district, Bangli regency. The research found 12 allele varieties from all the analyzed loci, which resulted in low genetic diversity (0.33 + 0.001). There are 4 combinations alleles of 4 loci that created12 various haplotypes, the greatest one is haplotype 1 (0.39), followed by haplotype 2 (0.16), haplotype 7 (0,12) and the smallest one is haplotype 6 (0,10). On the other hand, eight other alleles found on the smaller frequencies. Two similar allele combinations were also found on DNA profiles of Celagi clan, which is also belong to the similar family, Pasek Catur Sanak Bali Mula. The two allele are haplotype 6 and 5 found in both, Pasek Kayuan and Pasek Celagi. The haplotype 6 as dominant allele of Celagi clan by adoption, haplotype 5 however, the frequency was the lowest. This allele only detected in one member of each clan due to mutation.

RAGAM ALEL DAN KEKUATAN PEMBEDA LIMA LOKUS MIKROSATELIT DNA AUTOSOM MASYARAKAT SUKU BATAK DI DENPASAR DAN BADUNG I Ketut Junitha; Yossy Carolina Carolina
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 6 (2016): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Five loci of microsatellites DNA autosomal that is: D2S1338, D13S317, D16S539, D18S351, and D19S433 used to determination kind of allele and power of discrimination each locus of Batak ethnic in Denpasar and Badung. DNA sample were amplified used five pairs of primer D2S1338, D13S317, D16S539, D18S351, and D19S433 loci in PCR machine used PCR mix Invitrogen with annealing temperature range of 48-56oC. The result of this research was found 54 alleles. The most allele was found on D2S1338 locus, 13 alleles followed by D18S351, 12 alleles, D13S317 and D19S433 locus each 10 alleles and the least one is 8 allele on D16S539 locus. The highest of power of discrimination that is 0,984 D2S1338 locus followed by D18S351, D19S433, D16S539 and of D13S317 0,973, 0,956, 0,949, and 0,947 respectively. The five loci in this studied had many kind of alleles and high degree of power discrimination value so that loci is useful to forensic purpose for Batak society in Bali.

KARAKTER MORFOLOGI BERAS SEBAGAI PEMBEDA VARIETAS PADI Dicky Frengky Hanas; Eniek Kriswiyanti; I Ketut Junitha
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 7 (2017): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2017.v07.i01.p04

This study aims to determine the morphological character of white, red and black rice as a differentiator ofrice varieties. This research uses 10 rice samples which are 6 local rice of Kupang, 2 varieties of local superior riceand 2 Italian rice. The method used is direct observation of both qualitative and quantitative characteristics of rice.Long grain size of rice is a character that can indicate the distinguishing of local rice, domestic superior and ricefrom abroad. Character of rice morphology is a character very easily observed in knowing the difference of ricevarieties. Character of rice morphology are easily characters observed in knowing the difference of rice varieties.Characters difference can be used to determine the adulteration of rice mixed.

IDENTITAS GENETIK KETURUNAN SOROH PANDE DI KECAMATAN SERIRIT, BULELENG BERDASARKAN DNA MIKROSATELIT KROMOSOM Y I Wayan Arya Mahardika; I Ketut Junitha; Inna Inna Narayani
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 6 (2016): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2016.v06.i01.p01

The Balinese clans embraced patrilineal system.They divided into groups of soroh or clans which means respect for ancestors. Pandeclan is a unique soroh which comes from ancestors who have expertise in memande. The genetic identityof this clan in Bali is unknown.Therefore the aim of this study is to find out the genetic identity from the varieties of allelic diversity which found on Pande community inside and outside Seririt using microsatellite DNA analysis of Y chromosome, namely DYS19, DYS390, DYS393, and DYS395. Resultsof this study have foundthat in Pande clan there are 21 varieties of allelic diversity and 25 haplotypes with the average heterozygosity of four loci is 0.72 ± 0.03. The dendogram indicates that Pande clanfrom district of Seririt did not cluster based on it’s dadia.

Co-Authors A.A. Gde Lanang Adelina Arifiani Purnomo Agriani Dewinta Anastesya Hartika Nur Saputri Ayu Saka Laksmita W Bagas Susilo Bagus Alit, Ida Bambang Suryobroto Bram Setyadji Budiningsih, Desak Nyoman Cakra Diarsa Chandra Yowani Christian Subagya Gunardi Dewa Ayu Sri Ratnani Dwi Ariani Yulihastuti ENIEK KRISWIYANTI Eswaryanthi Kusuma Yuni Fita Efi Rafelina Siallagan Graciella Stevani Gulo Gusti Ayu Putri Arnila Hanas, Dicky Frengky I Gde Suryadi I Gede Yeyen Suharta I Gusti Agung Eka Saraswati I K. Sukardika I Ketut Sundra I Made Sara Wijana I NYOMAN MANTIK ASTAWA I Wayan Arya Mahardika Ida Ayu Manik Damayanti Ida Bagus Alit Ida Bagus Bajing Agastya Ida Bagus Made Suaskara IMA Gelgel Wirasutha Inna Narayani IRIANI SETYAWATI Kezia Artanauli Purba Lucia Emy Octavia MADE PHARMAWATI Maya Agustina Maya Agustina Meitini Proborini Wahyuni Muhamad Masyrur NI KADEK YUNITA SARI Ni Luh Putu Ria Puspitha Ni Luh Putu Rika Sugiantari Ni Luh Watiniasih Ni Made Puspawati Ni Made Rai Suarni Ni Made Suartini Ni Made Suartini Ni Nyoman Wirasiti Ni Putu Puniari Eka Putri, Ni Putu Puniari Eka Ni Putu Putri Wulandari Putri Arie Prasetyoningrum Putri Karenina Siregar Putu Rosi Setyari SANG KETUT SUDIRGA Septiasari, Ni Putu Senshi Tyara Tyara Victor Peter Poli Yan Ramona Yossy Carolina Carolina YOSSY CAROLINA UNADI Yulia Mirayanti

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