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All Journal HAYATI Journal of Biosciences INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES METAMORFOSA Journal of Biological Sciences Jurnal Farmasi Udayana INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Biologi Udayana Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology SIMBIOSIS Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Kajian Bali Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Journal of Tropical Ethnobiology International Journal of Applied Science and Sustainable Development (IJASSD) International Journal of Scientific Multidisciplinary Research (IJSMR)

I Ketut Junitha

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 1918405

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mechanical Engineering Medicine & Pharmacology Social Sciences

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VARIATION OF FINGERPRINT PATTERNS OF KAILINESE AND TORAJANESE TRIBES IN PALU AND SIGI REGENCY, CENTRAL SULAWESI Graciella Stevani Gulo; I Ketut Junitha; Iriani Setyawati
SIMBIOSIS Vol 8 No 2 (2020)
Publisher : Jurusan Biologi Universitas Udayana

A research on the variation of fingerprint patterns of Kailinese and Torajanese tribes have been done in Palu and Sigi Regency, Central Sulawesi. The aims of this research were to determine the variation of fingerprint patterns, Dankmeijer Index, Furuhata Index and Total Ridge Count of Torajanese and Kailinese. The population was divided into three groups, namely the Kaili Da'a (Kaili tribe which has phenotypic characteristics resembling the Australomelanesoid race), other Kaili groups and the Toraja tribe belonging to the Mongoloid race. The samples of fingerprints were taken by using the purposive sampling method with a total of 180 probandus, consisted of 60 people (30 males and 30 females) in each ethnic group. The tips of ten fingers were rubbed with paper that had contained 4B pencil rubbing, fingerprints were taken using clear tape and then the tape affixed on a probandus form. Data was analysed by Chi-square test and Student-t test. The fingerprint analysis showed that there were four fingerprint patterns found among the Kailinese and Torajanese tribes. The highest whorl frequency was found in the Torajanese at 50.17%, whereas Da'a and other Kaili groups had higher loop frequencies. Whorl type analysis showed simple whorl was the type most commonly found in all three populations. Furthermore, double loop whorl and the least frequency was the central pocket whorl. The Furuhata Index values ??for the Toraja, Kaili and Kaili Da'a tribes respectively were 102.73; 57,37; and 95.06 while the Dankmeijer Index values ??were 1.99; 5,61; and 2.77. The average of total ridge count in the Toraja, Kaili and Kaili Da'a tribes respectively were 134; 128.08 and 133.56.

Penanda DNA mikrosatelit Kromosom-Y untuk Penelusuran Soroh-soroh/Kawitan Masyarakat Bali I Ketut Junitha
Journal of Tropical Ethnobiology 2021: Prosiding Seminar Nasional PMEI V 2020
Publisher : The Ethnobiological Society of Indonesia

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Soroh is a Balinese term for a clan in Indonesian. Each soroh is a group of Balinese Hindus who believe they are descended from one common ancestor, and has one main temple as a place of worship to the ancestors, which is called the kawitan temple. Each main soroh can be divided into several sub-soroh based on the male lineage of the main ancestor. Each sub-soroh also has a temple for their ancestors’ worship which is also called pura kawitan. Therefore, there are known subsoroh and pura kawitan main temples, whose members are a combination of sub-soroh and sub-sections. Soroh Catur Sanak Bali Mula believes he is a descendant of Mpu Kamereka which developed into four sub-soroh, namely Kayu Selem, Celagi, Kayuan, and Terunyan. The family system in Bali adheres to a patrilineal descent where all children from one family will follow the cheer of their father. For Balinese Hindus, knowing their soroh or kawitan temple is an obligation to be able to offer prayers to their kawitan temple as a form of respect for each offspring to their ancestors. Losing traces of ancestors (kawitan) is believed to be a major problem in the family life. Based on the belief that each soroh is descended from one ancestor and the family system in Bali adheres to a patrilineal lineage, soroh or kawitan tracing can be carried out by DNA analysis with Ychromosome microsatellite markers. DNA or genes on the Y chromosome that are only present in males will be passed from males to only males and so on. Therefore, the Y-chromosome microsatellite profile can be used as a search tool for Soroh or Kawitan in Balinese society.

Hubungan Genetika Pria Masyarakat Sembiran dan Tri Wangsa di Bali I Ketut Junitha; Bambang Suryobroto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 1 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.1.185

Balinese community has evolved since the prehistoric era. The present community is structured into Hinduism caste (Brahmana, Kesatria, Wesia and Sudra), but remnants of their ancient communities, collectively called Bali Aga, have not undergone this structure. The first three castes of modern Balinese that comprises Tri Wangsa gentry were derived from JavanesekingdomofMajapahit(1343 AD). One of Bali Aga communities is Sembiran of Northern Bali. Using chromosomal microsatellite DNA, allelic variability of Sembiran and Tri Wangsa communities was reported. Both of them had the same high frequency of alleles which suggested genetic intermixing or sharing of common ancestor. However, a few alleles were unique for each community. This uniqueness was related to patrilineal system of Balinese community and to high mutation rate of Y chromosomal microsatellite DNA.

Laju dekomposisi bangkai mencit (Mus musculus) yang dikubur selama empat minggu pada media tanah humus, kapur, dan pasir pantai Cakra Diarsa; I Ketut Junitha; I Ketut Sundra
Jurnal Biologi Udayana Vol 26 No 1 (2022): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2022.v26.i01.p08

Dekomposisi bangkai terjadi segera setelah organisme mati mulai dari dekomposisi tingkat jaringan hingga tingkat molekuler. Laju dekomposisi bangkai hewan yang dikubur dipengaruhi oleh tiga faktor yaitu faktor fisik, kimia, dan biologi dari media yang digunakan mengubur bangkai. Faktor fisik berupa struktur, porositas, dan kelembaban dari media. Faktor kimia berupa pH, konsentrasi natrium, nutrien, dan oksigen yang terkandung pada media penguburan. Faktor biologi berupa jumlah dan komposisi dari koloni bakteri, invertebrata, dan flora yang hidup pada media. Tujuan dari penelitian ini adalah untuk mengetahui apakah terjadi perbedaan kecepatan dekomposisi bangkai pada media penguburan yang berbeda. Penelitian ini dilakukan dengan menggunakan bangkai mencit sebanyak 36 ekor yang dikuburkan pada tiga media berbeda yaitu tanah humus, pasir pantai, dan tanah kapur di dalam toples plastik. Masing-masing sebanyak 12 toples diisi dengan media yang sama sebagai ulangan. Bangkai mencit diamati selama 28 hari dimana tiap tujuh hari dilakukan penimbangan terhadap berat bangkai mencit dengan timbangan digital dan kondisi fisik dari bangkai mencit dicatat dan diskoring. Laju dekomposisi bangkai mencit ditunjukkan dari perbedaan rata-rata penurunan berat bangkai mencit (%) tiap minggu pada masing-masing media. Metode analisis data dilakukan dengan uji ANOVA. Hasil dari penelitian didapatkan adanya perbedaan penurunan berat dan nilai skor kondisi fisik bangkai mencit pada tiap media penguburan. Kesimpulan penelitian ini adalah waktu dan perbedaan media penguburan berpengaruh pada penurunan berat dan nilai skoring kondisi fisik bangkai mencit.

THE IMPACT OF LAUNDRY DETERGENT AS A METHOD OF WASHING TOWARDS SPERM DNA EXTRACTION RESULT ON COTTON FABRIC Christian Subagya Gunardi; I Ketut Junitha; Inna Narayani
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

The presence of spermatozoa DNA is reliably counted as a solid evidence when dealing with rape cases. However, police department often finds difficulties to obtain it, mainly because it has been destroyed by the perpetrator. Such act can be done by washing semen stains on the clothing with detergent. The purpose of this research is to analyze the impact of detergent as a washing property towards spermatozoa DNA extract on a cotton fabric. Observed variables in this study are quantity and quality of spermatozoa DNA extract from semen stain on cotton fabric. Samples are divided based on two separate treatments; detergent-washed and unwashed. Furthermore, samples are stored with different storage time. This research applied two methods; DNA extraction using kit (Roche) and DNA quantification using spectrophotometer Nanodrop. The research was held at Biomedic Laboratory, Faculty of Medicine, Udayana University, Denpasar with ethical clearance. Seminal plasma sampel was obtained from a donor with informed consent. This research showed that seminal stain washing on cotton fabric doesn't remove the DNA until two-week-storage time.

KUANTIFIKASI DNA PADA MAHASISWA PEROKOK DAN BUKAN PEROKOK DI UNIVERSITAS NEGERI MEDAN KECAMATAN MEDAN TEMBUNG KOTA MEDAN PROVINSI SUMATERA UTARA Kezia Artanauli Purba; I Ketut Junitha; Ni Nyoman Wirasiti
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Individual identification is very important in forensics. DNA can be obtained from all parts of the body with the same profile in everyone. Oral mucosal epithelium is one of the sources of DNA that is often used to examine individuals because it is taken using a harmless swab method. Smoke is one of the behaviors that are mostly done by young people or teenagers. Cigarette smoke affects the cells of the oral mucosa because it is a free radical. Free radicals are reactive oxygen compounds which are compounds with unpaired electrons. Deoxyribonucleic acid (DNA) is a nucleic acid polymer that is systematically arranged and is a carrier of genetic information that is passed on to offspring. This study aims to determine the quality and quantity of DNA in non-smoking and smoking students at the State University of Medan, Medan Tembung District, Medan City, North Sumatra Province. Samples were taken by swab method, namely mucosal epithelium from 60 probands consisting of 30 smoker proband and 30 non- smoking proband aged 18-22. The cheek in the probandus is swabbed from the back to the front in one direction. In this study, DNA extraction was carried out using a chelex solution which aims to separate DNA from protein, test the quantity of DNA with a spectrophotometer and test the quality of DNA with agarose gel. The results showed that the average DNA quantity in the oral mucosal samples was 1.96 ng/µL in the smokers proband and 6.92 ng/µL in the non-smoker proband and. The results of the quality test using electrophoresis on agarose gel showed that smoking students had thin bands of fluorescence and in some samples no bands of fluorescence at all. Meanwhile, in the probandus of non-smoker students, there were several samples that showed thin bands of luminescence and stains.

THE ETHNOBOTANY OF SENSE DISEASE MEDICAL PLANT USED BY NGIS MANGGIS COMMUNITY KARANGASEM IN BALI, INDONESIA Dewa Ayu Sri Ratnani; I Ketut Junitha; Eniek Kriswiyanti; Desak Nyoman Budiningsih
International Journal of Applied Science and Sustainable Development (IJASSD) Vol. 4 No. 2 (2022): International Journal of Applied Science and Sustainable Development (IJASSD)
Publisher : Lembaga Penelitian dan `Pengabdian Kepada Masyarakat (LPPM)

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The Ngis Manggis community has ethnobotanical knowledge in utilizing plants from the forest for traditional medicine. Local people have little knowledge of managing forests sustainably. This study aimed to identify plants used for sense disease medicinal by the Ngis Manggis community, including species, family, local names, parts of plants used, processing method, usage method, obtained sources, and the Index of Cultural Significance (ICS). Qualitative method to obtain data on the local names of plants used for the sense disease medicinal. Purposive and Snowball sampling methods were applied to collect key informants through semi-structured interviews and moderate participation. Data analysis was qualitative and quantitative. The results showed that 55 species were distributed in 32 families to treat 17 diseases dominated by Fabaceae (6 species). Most of them were harvested from wild 29 (52.72%). The most widely used part of the plants is the leaf. Crushing is the most widely used preparation method by the community. The greatest number of medicinal uses of Ngis Manggis community is smeared. Based on the results of the ICS analysis, the highest ICS value is Arenga pinnata L. (61).

FORENSIC IDENTIFICATION BASED ON PRIMARY AND SECONDARY XAMINATION AS DETERMINATION OF VICTIMS IDENTIFIERS: NORTH LUWU FLOOD CASE STUDY, SOUTH SULAWESI Anastesya Hartika Nur Saputri; I Ketut Junitha; Ida Bagus Made Suaskara
SIMBIOSIS Vol 11 No 1 (2023)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2023.v11.i01.p01

Forensic identification in mass disasters is carried out to determine the identity of the deceased. The identification process is important to provide psychological peace to the family with the certainty of the identity of the victim. Forensic identification methods are divided into primary examination (fingerprints, teeth, DNA) and secondary examination (medical characteristics and properties). This case study aims to determine the identity of flash flood victims in North Luwu, South Sulawesi based on primary and secondary examinations, determine the effect of the duration of the discovery on identification, and determine the further examination of unidentified victims. The research was carried out at the South Sulawesi Police Biddokes DVI post (RS. Hikmah Masamba) on 16-23 July 2020 and DNA examination was carried out at the Cell and Molecular Biology Laboratory, Faculty of Medicine, Udayana University accompanied by ethical clearance in June-August 2021. The material in In this study was the victims of the flash flood on the North Luwu, there were 40 victims, consisting of 31 victims who had been declared identified, 6 victims were missing or have not been found, and 3 victims had not been identified. Victims who are declared identified are based on the results of the examination (post mortem) which are compared with ante mortem data in accordance with the identification principle of Interpol (International Criminal Police Organization). Thirty-one victims identified were subjected to primary and/or secondary examination, of which 20 victims were identified by secondary examination and 11 victims were identified by primary and secondary examination, and 3 victims who had not been identified were conducted to DNA primary examination. Based on the results of DNA examination, it was found that the suspect's family was most likely a relative with one of the victims with a PM number 008.

Kuantitas Dan Kualitas DNA Hasil Ekstraksi Dari Bercak Darah Pada Pisau Pasca Paparan Sinar Ultraviolet Dan Matahari Putri Arie Prasetyoningrum; I Ketut Junitha; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p19

Examination of Deoxyribonucleic Acid is the primary identification method in forensic cases. Blood stains found at the scene of a crime are often affected by various environmental factors, one of which is ultraviolet light from the sun. Purpose of this study is to compare the quantity and quality of DNA extracted from blood stains on the blade after exposure to ultraviolet light and the sun for 0, 15 and 30 days. The method used is a factorial design: light source (UVA, indirect sunlight and direct sunlight); and duration of treatment (0, 15 and 30 days). Blood stains are made by dripping a 50?L of human blood on one side of the blade and then given treatment. DNA analysis includes: extraction with Chelex 5%; DNA quantity and quality test with SimpliNano spectrophotometer; total DNA quality test with agarose gel electrophoresis; and PCR. Quantitative data analysis using Univariate test followed by Duncan test. The results showed that the quantity of DNA in samples exposed to UVA and sunlight (directly) increased on the 15th day due to DNA fragmentation while samples exposed to sunlight (indirectly) decreased the quantity of DNA with the duration of treatment. DNA quality in the form of DNA purity produces an extract of DNA that is not pure. The total DNA quality with agarose gel electrophoresis on all samples showed that the longer the treatment (0, 15 and 30 days) resulted in a dimmer or thinner band of DNA luminescence with smears.

Optimasi digesti enzim restriksi untuk deteksi mutasi daerah D-loop DNA mitokondria dengan metode PCR-RFLP Ni Putu Senshi Septiasari; I Ketut Junitha; Ni Nyoman Wirasiti
Jurnal Biologi Udayana Vol 27 No 1 (2023): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2023.v27.i01.p07

Metode PCR-RFLP merupakan salah satu metode untuk deteksi mutasi pada daerah D-loop DNA mitokondria. Metode ini menggunakan enzim restriksi untuk dapat memotong DNA mitokondria dan menghasilkan ukuran fragmen DNA yang berbeda-beda. Enzim restriksi memerlukan kondisi yang optimal untuk melakukan pemotongan DNA. Penelitian ini bertujuan untuk mengetahui kondisi optimal enzim restriksi agar dapat melakukan digesti pada daerah D-loop DNA mitokondria. Optimasi dilakukan dengan membuat dua kombinasi formula digesti (formula 1 dan 2) dan empat macam waktu digesti (2 jam, 4 jam, 6 jam dan overnight). Hasil penelitian menunjukkan optimasi dari lima macam enzim restriksi (HaeIII (BsuRI), HindIII, HinfI, MboI dan HpyP31 (DdeI)) didapatkan bahwa ada perbedaan formula dan waktu digesti tergantung dari jenis enzim. Enzim HaeIII(BsuRI), HinfI dan MboI menunjukkan formula 2 merupakan formula optimal, sedangkan enzim HpyP31 (DdeI) formula 1 merupakan formula yang optimal. Waktu digesti 2 jam menunjukkan hasil optimal pada Enzim HaeIII(BsuRI), MboI dan HpyP31 (DdeI), sedangkan enzim HinfI waktu digesti optimal adalah 4 jam. Enzim HindIII tidak mendapatkan hasil potongan fragmen DNA setelah digesti, maka enzim HindIII tidak memiliki situs pemotongan pada daerah D-loop DNA mitokondria.

Co-Authors A.A. Gde Lanang Adelina Arifiani Purnomo Agriani Dewinta Anastesya Hartika Nur Saputri Ayu Saka Laksmita W Bagas Susilo Bagus Alit, Ida Bambang Suryobroto Bram Setyadji Budiningsih, Desak Nyoman Cakra Diarsa Chandra Yowani Christian Subagya Gunardi Desak Nyoman Budiningsih Dewa Ayu Sri Ratnani Dewa Ayu Sri Ratnani Dwi Ariani Yulihastuti ENIEK KRISWIYANTI Eswaryanthi Kusuma Yuni Fita Efi Rafelina Siallagan Graciella Stevani Gulo Gusti Ayu Putri Arnila Hanas, Dicky Frengky I Gde Suryadi I Gede Yeyen Suharta I Gusti Agung Eka Saraswati I K. Sukardika I Ketut Sundra I Made Sara Wijana I NYOMAN MANTIK ASTAWA I Wayan Arya Mahardika Ida Ayu Manik Damayanti Ida Bagus Alit Ida Bagus Bajing Agastya Ida Bagus Made Suaskara IMA Gelgel Wirasutha Inna Narayani IRIANI SETYAWATI Kezia Artanauli Purba Lucia Emy Octavia MADE PHARMAWATI Maya Agustina Maya Agustina Meitini Proborini Wahyuni Muhamad Masyrur NI KADEK YUNITA SARI Ni Luh Putu Ria Puspitha Ni Luh Putu Rika Sugiantari Ni Luh Watiniasih Ni Made Puspawati Ni Made Rai Suarni Ni Made Suartini Ni Nyoman Wirasiti Ni Putu Puniari Eka Putri, Ni Putu Puniari Eka Ni Putu Putri Wulandari Ni Putu Senshi Septiasari Putri Arie Prasetyoningrum Putu Rosi Setyari SANG KETUT SUDIRGA Tyara Tyara Victor Peter Poli Yan Ramona Yossy Carolina Carolina YOSSY CAROLINA UNADI Yulia Mirayanti

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