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All Journal HAYATI Journal of Biosciences Torani Journal of Fisheries and Marine Science Microbiology Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Jurnal Sain Peternakan Indonesia BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Tropical Animal Science Journal Indonesian Aquaculture Journal Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Aquacultura Indonesiana Journal of Tropical Ethnobiology Unri Conference Series: Community Engagement Current Research on Biosciences and Biotechnology International Journal of Islamic Education, Research and Multiculturalism (IJIERM) Makara Journal of Science OKTAL : Jurnal Ilmu Komputer dan Sains BULLET : Jurnal Multidisiplin Ilmu Bioeksperimen Biodiversitas Journal of Biological Diversity
Abinawanto
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Indonesia
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Pengaruh Variasi Konsentrasi Kuning Telur terhadap Abnormalitas Sperma Puntius bramoides Pascakriopreservasi 48 Jam Pertiwi, Primasari; Abinawanto
OKTAL : Jurnal Ilmu Komputer dan Sains Vol 4 No 06 (2025): OKTAL : Jurnal Ilmu Komputer Dan Sains
Publisher : CV. Multi Kreasi Media

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Abstract

The study aims to determine the effect of several concentrations of egg yolk as an effective natural cryoprotectant for cryopreservation and to analyze the quality of spermatozoa of Puntius bramoides fish (lukas fish) 48 hours after cryopreservation. This study used a Completely Randomized Design (CRD) with 9 treatments and 4 replications. The concentrations of egg yolk used as cryoprotectants were 0% (negative control), 5%, 7%, 9%, 11%, 13%, 15%, 17%, and the use of Carboxymethyl Cellulose (CMC) 1% as a positive control. The results showed that the use of several concentrations of egg yolk as a natural extracellular cryoprotectant was able to significantly affect (P <0.005) the abnormalities of spermatozoa of lukas fish 48 hours after cryopreservation. The use of 9% egg yolk concentration is the optimum concentration in reducing the percentage of abnormality of sperm in lukas fish with an average percentage obtained of 26.13 ± 1.49%.
Efek Kuning Telur Terhadap Motilitas Sperma Ikan Lukas (Puntius bramoides Val) Pascakriopreservasi Pertiwi, Primasari; Abinawanto; Farisi, Salman
BULLET : Jurnal Multidisiplin Ilmu Vol. 3 No. 1 (2024): BULLET : Jurnal Multidisiplin Ilmu
Publisher : CV. Multi Kreasi Media

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Abstract

The research aims to determine the effect of several concentrations of egg yolk as an effective natural cryoprotectant for cryopreservation and to analyze the quality of lukas fish spermatozoa (Puntius bramoides Val) 48 hours after cryopreservation. This research used a 9 treatments and 4 replications. The concentration of egg yolk used as a cryoprotectant was 0% (negative control), 5%, 7%, 9%, 11%, 13%, 15%, 17%, and 1% Carboxymethyl Cellulose (CMC) was used as a positive control. The results showed that the use of several concentrations of egg yolk as a natural extracellular cryoprotectant was able to significantly influence (P<0.005) the quality of Lukas fish spermatozoa 48 hours after cryopreservation. The use of egg yolk concentration of 9% is the optimum concentration in maintaining the motility of Lukas fish spermatozoa with the average percentage obtained each being 80.45 ± 0.93%.
A Comparative Study of Penicillin G Acylase Expression in Two Escherichia coli Strains: BL21 (DE3) and Arctic Express Cendana, Kartika Sari; Wulandari, Sri Rezeki; Sabbathini, Gabriela Christy; Ulfah, Maria; Achnafani, Dini; Abinawanto; Aniqah, Sunni Sofiah; Helianti, Is; Nurhayati, Niknik
HAYATI Journal of Biosciences Vol. 33 No. 3 (2026): May 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.3.596-604

Abstract

The growing demand for semisynthetic beta-lactams has directed attention towards enzymes, specifically Penicillin G Acylases (PGAs), for their potential in synthesizing these antibiotics. This study delves into the expression of Achromobacter xylosoxidans PGA (AxPGA) in Escherichia coli, with a focus on enhancing the yield of active PGA, often constrained by a complex maturation process. The optimization of PGA expression included variations in IPTG concentration and the addition of CaCl2. Furthermore, the study compared PGA expression in E. coli BL21 (DE3) with that in E. coli Arctic Express (DE3), capable of co-expressing chaperones (chaperonin Cpn60 and Cpn10). Induction with 0.5 mM IPTG resulted in the highest hydrolytic activity in both strains, with Arctic Express (AE) exhibiting significantly higher activity due to improved folding facilitated by cold-adapted chaperonins. Alongside optimal IPTG induction, the addition of 10 mM CaCl2 in the culture media significantly increased PGA activity in both strains, highlighting that Ca2+ supplementation is an effective strategy to enhance the yield of functional PGA. Subcellular fractionation demonstrates that the periplasmic fraction yielded higher volumetric and specific activities compared to the cytoplasmic fractions in both E. coli strains, highlighting the importance of periplasmic processing for PGA maturation. This suggests that extracting the periplasmic fraction is an effective strategy for recovering active PGA while avoiding or reducing contamination either from co-expressed cytoplasmic chaperones or other intracellular proteins. These findings emphasize that induction strategy, ionic stabilization, and host strain selection play synergistic roles in increasing active recombinant PGA expression.
Impact of Meat Processing on DNA Quantity, Purity, and Integrity in Commercial Pork Products Assessed by TaqMan qPCR Rahayu, Ernawati Puji; Abinawanto
Bioeksperimen: Jurnal Penelitian Biologi September 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.11031

Abstract

TaqMan qPCR analysis for detecting adulteration in processed meat products requires DNA with a high quantity, purity, and integrity. This study aims to evaluate the quantity, purity, and DNA integrity of various commercial pork products focusing on their ability to amplify the Cyt b gene. Fresh pork was used as a positive control, while five processed products - jerky, floss, meatball, canned corned, and smoked pork - were extracted for analysis. The DNA concentration and purity then measured, visualized using gel electrophoresis, and subjected to qPCR amplification. Statistical analysis of DNA concentration, purity, and ct value was carried out using one-way ANOVA followed by the Tukey post hoc test. The results showed significant differences in concentration, purity, and Ct values between all samples and positive controls (P˂0.05). Meanwhile, the Tukey test showed that all samples and fresh pork concentration differed significantly (P˃0.05) except between canned corned and fresh pork (P˂0.05). The purity was significantly different between the positive controls with pork meatball and smoked pork (P˂0.05), while the Ct​ value of all samples and positive controls were significantly different (P˂0.05) except for fresh pork and jerky (P˃0.05). All the commercially processed products experienced DNA fragmentation. Meat processing affects DNA concentration, purity, genomic integrity, and the amplification ability of qPCR.
Spatial Model of Suitability Area Development for Marine Ecotourism in East Sumba Regency Dewi Susiloningtyas; Abinawanto Abinawanto; Tito Latif Indra; Zefanya Cellina Aurawicona; Bintang Mahakarya Sembahen; Adam Heray Hutabarat
Torani Journal of Fisheries and Marine Science Vol. 9 No. 2 (2026): VOLUME 9, NOMOR 2, JUNE 2026
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35911/torani.v9i2.45259

Abstract

The coastal region of East Sumba Regency possesses significant natural resources with high productivity, including both renewable and non-renewable resources. Renewable coastal ecosystems such as mangroves, coral reefs, seagrass beds, and fisheries play an important role in supporting local livelihoods while offering strong potential for marine ecotourism development. Several coastal areas, including Londa Lima Beach, Walakiri Beach, Tapil Beach, Puru Kambera Beach, and Wanga Beach, exhibit diverse physical and social characteristics that can support ecotourism activities. This study aims to assess the sustainability of coastal resource utilization in East Sumba Regency by evaluating the suitability of marine ecotourism development through the integration of ecological, economic, social, cultural, and institutional dimensions. A quantitative approach was employed using a scoring-based method derived from field surveys, data processing, and descriptive analysis. The analysis applies several physical parameters, including beach type, coastline length, seabed material, current velocity, hazardous biota, land cover, and freshwater availability. Social aspects are assessed using the 4A tourism framework: attraction, accessibility, amenities, and ancillary services. The results provide insights to support sustainable marine ecotourism development in East Sumba Regency. The result shows that The Tourism Suitability Index (TSI) for Tapil Beach is 1.15, Wanga Beach with a score of 1.41, Walakiri Beach is 2.09, Puru Kambera  Beach with a score of 1.38,  and Londa Lima Beach with a score of 2.0. Based on the TSI analysis, the Walakiri Beach and Londa Lima Beach are suitable locations for beach-based ecotourism development 
Short Communication: Biodiversity of the Gaga chicken from Pinrang, South Sulawesi, Indonesia based on the bioacoustic analysis and morphometric study ABINAWANTO ABINAWANTO; PIPIH SUNINGSIH EFFENDI
Biodiversitas Journal of Biological Diversity Vol. 18 No. 4 (2017)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d180440

Abstract

Abinawanto, Effendi PS. 2017. Biodiversity of the Gaga chicken from Pinrang, South Sulawesi, Indonesia based on the bioacoustic analysis and morphometric study. Biodiversitas 18: 1618-1623. Gaga chicken (Gallus gallus domesticus) is the local ornamental chicken originated from South Sulawesi, Indonesia, being kept for the crows. Gaga chicken which also called “ayam ketawa” (“laughing chicken”) has the unique crowing song, which sounds like human laughing. Gaga chicken which has long and fast crowing song is called ‘dangdut’ type, while the short and slow crowing song is named ‘slow’ type. The objective of present study is to investigate the biodiversity of Gaga chicken from Pinrang, South Sulawesi, Indonesia based on morphometric and bioacoustic characters. Twenty samples of Gaga chicken have been collected, consisted of eight ‘slow’ types and twelve ‘dangdut’ types, respectively. Data were analyzed by t-test using SPSS ver. 22. The result showed that ‘slow’ type was bigger than ‘dangdut’ type. Morphometric characters could be applied to determine the biodiversity of Gaga chicken.
Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis LULUT DWI SULISTYANINGSIH; MARLINA ARDIYANI; ABINAWANTO ABINAWANTO; ANDI SALAMAH
Biodiversitas Journal of Biological Diversity Vol. 19 No. 2 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190202

Abstract

Sulistyaningsih LD, Abinawanto, Ardiyani M, Salamah A. 2018. Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis. Biodiversitas 19: 364-368. Smilax spp. (Smilacaceae) has long been used as medicinal herbs especially in East Asia and North America as they were known to be rich in steroidal saponin. Pharmacological study has been carried out in Indonesia. This genus is widespread in Indonesia and fairly abundant in Java and has been known either as edible fruit or medicinal plants. Characteristics of Smilax as a dioecious plant with high morphological variations make it thorny in species identification. Various molecular approaches have been devised to overcome identification problems such as DNA barcoding. This study, therefore was conducted to analyze the DNA barcoding application for phylogenetic and identification of Smilax in Java. A total of 31 samples were used in this study including 19 accession numbers from NCBI GeneBank. The genus Ripogonum was used as the out-group in phylogenetic reconstruction. Samples were successfully extracted by CTAB method with some modifications. rbcL region was used as the DNA barcode showed sufficient variation and conserved flanks. Two unidentified specimens have high similarity with S. leucophyla and lies in the same clade. The phylogenetic tree constructed by Maximum Likelihood analysis. The result showed that the monophyletic of Smilacaceae consisted of four clades. The genus Heterosmilax nested with Smilax though with low bootstraps value. It supports the monogeneric status of Smilacaceae.
Short Communication: Biometric of freshwater crayfish (Cherax spp.) from Papua and West Papua, Indonesia ABINAWANTO ABINAWANTO; HANI HAMIDAH; ANOM BOWOLAKSONO; RURY EPRILURAHMAN
Biodiversitas Journal of Biological Diversity Vol. 19 No. 2 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190216

Abstract

Abinawanto, Hamidah H, Bowolaksono A, Eprilurahman R. 2018. Short Communication: Biometric of freshwater crayfish (Cherax spp.) from Papua and West Papua,, Indonesia. Biodiversitas 19: 489-495. Freshwater crayfish, Cherax spp. is a native crayfish species in Papua, Indonesia. Biometric information of this crayfish species in Indonesia is limited. Hence, this study aimed to provide these data. Specimens were collected from West Papua Province, i.e., from Uter Lake (Atinjo Subdistrict, Maybrat) and Seremuk River (Haha Village, Seremuk Subdistrict, South Sorong), respectively. Besides, the specimens were also collected from Papua Province in Baliem River at Pike Village, Hubukiak Subdistrict, and Wesaput Village, Wesaput Subdistrict, Jayawijaya. Data were analyzed using canonical discriminant function (CDF). The results showed that the longest carapace of Cherax spp. was 97.83 mm found in Baliem River at Pike Village. CDF analysis showed that there were three population groups of Cherax spp. from four locations. The first group originated from Uter Lake (Atinjo Subdistrict) and Seremuk River (Haha village). The second group was from Baliem River (Pike Village; Hubukiak Subdistrict, Jayawijaya and the third group from Baliem River (Wesaput Village; Wesaput Subdistrict)
Characterization of Sentani gudgeon, Oxyeleotris heterodon (Weber, 1907) at Sentani Lake, Papua, Indonesia based on truss morphometric ABINAWANTO ABINAWANTO; EKA DEWI SRIYANI
Biodiversitas Journal of Biological Diversity Vol. 19 No. 3 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190333

Abstract

Abinawanto, Sriyani ED, Bowolaksono A. 2018. Characterization of Sentani gudgeon, Oxyeleotris heterodon (Weber, 1907) at Sentani Lake, Papua, Indonesia based on truss morphometric. Biodiversitas 19: 1013-1020. The study concerning the morphometry of Sentani gudgeon (Oxyeleotris heterodon, Weber 1907) was done from August 2016 until April 2017 in Sentani Lake, Jayapura District, Papua Province, Indonesia. The objectives of the study were to find out the diversity of the fish using truss morphometry approach. A total 56 individual sampled from three sampling sites were characterized for their 26 truss morphometric characters. The result showed that there were differences on morphometric characters of Sentani gudgeon. The highest correlation value found in character B2 and D5, which represented the dorsal portion and the tail fin of the fish body respectively. All variables showed significant different between each location (the p-value &lt;c0.05). The canonical diagram resulted of 26 truss morphometric characters illustrated three separated clusters which discriminated the three populations based on sampling sites. A genetic distance analysis among Sentani gudgeon indicated that the fish originated from Putali Village was morphologically closed to the fish population from Sosiri. The truss morphometry technique can be used to identify the diversity of Sentani gudgeon in Sentani Lake.
Short Communication: Variation in vocal cord morphometric characters among dangdut type and the slow type Gaga Chicken ABINAWANTO ABINAWANTO; ALFI SOPHIAN; PIPIH SUNINGSI EFENDI; TITIN SISWANTINING
Biodiversitas Journal of Biological Diversity Vol. 19 No. 5 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190542

Abstract

Abinawanto, Sophian A, Effendi PS, Siswantining T. 2018. Short Communication: Variation in vocal cord morphometric characters among dangdut type and the slow type Gaga Chicken. Biodiversitas 19: 1902-1905. Gaga chicken is one of the ornamental chicken originating from Sidendreng and Rapang (South Sulawesi). Gaga chicken has a unique crowing sound, like people laugh. Gaga’s chicken which has a long and fast crowing sound known as the dangdut type, while those with short and slow crowing sound are known as slow type. Study was conducted in Pinrang (South Sulawesi), where one of the pure strains are located. Samples were collected from Kanie, Bullo, Macege, Rappang, and Sidenreng. The purpose of study was to determine the characteristics of the vocal cord morphometric among the dangdut type and the slow type of Gaga’s chicken. All of the morphometric data were recorded and analyzed by Mean Test, using SPSS (version 22). The results showed that there was no significant difference (a = 0.010) among the dangdut type and the slow type based on syrinx morphometric. Meanwhile, according to the trachea muscle morphometric the results showed that the trachea muscle of dangdut type was longer than the slow type (a &lt; 0.010). In addition, either the right or the left trachea muscle of dangdut type was longer than either the right or the left trachea muscle of the slow type (a &lt; 0.010).
Co-Authors A. SOFYAN A. Sofyan A. Sofyan A. SOPHIAN ABDUL BASITH Abdul Basith Achmad Dinoto Achmad Dinoto Achnafani, Dini Adam Heray Hutabarat Ade Sunarma Advent Tri Joy Simamora Ady Suryawan Afifa Hasna Agung Sudaryono Agus Haryadi Ahmad Aulia Jusuf ALFI SOPHIAN ALFI SOPHIAN ALFI SOPHIAN Alimuddin ANANG HARI KRISTANTO Andi Salamah Aniqah, Sunni Sofiah Anita Mayasari ANOM BOWOLAKSONO Arief Boediono Arief Boediono ASRI SULFIANTI Astari Dwiranti Ayu Mulia Sundari Ayu Mulia Sundari Azzizah, Isma Nur Bayu , Mariana D. Bintang Mahakarya Sembahen Cendana, Kartika Sari Dewi Susiloningtyas Diki Nurul Huda Djamhuriyah S. Said DODDY IRAWAN SETYO UTOMO EKA DEWI SRIYANI ELISABETH LOVIAN ULI BASA ELIZABETH NOVI KUSUMANINGRUM Endang C. Purba Endang Sukara ENI KUSRINI Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini, Eni Erma Primanita Hayuningtyas Fadhilah FADHILLAH FADHILLAH Farisi, Salman Fitrianingrum, Nisa Funahashi, Hiroaki H. JULENDRA H. Julendra HANI HAMIDAH Hidayat Hidayat Hiroaki Funahashi Huriyah Adani Saoemi INA ERLINAWATI IS HELIANTI is helianti IS HELIANTI Ita M. Nainggolan Jatna Supriatna L. Istiqomah Lina Mulyawati LULUT DWI SULISTYANINGSIH Lulut Dwi Sulistyaningsih LUTHFIRALDA SJAHFIRDI M. F. Karimy M. F. Karimy Maria Ulfah Maria Ulfah Mariana D. Bayu MARIANA D. BAYU INTAN Marina Silalahi MARLINA ARDIYANI Marlina Ardiyani Maylasari, Roospita MILANI ANGGIANI Nataniel, Jocelyn NIKEN DHARMAYANTI NIKNIK NURHAYATI NIKNIK NURHAYATI Nisyawati Nova Anita Noviyanti, Rintis Nurfitriani, Risya Nurul Muhammad Prakoso Odang Carman PIPIH SUNINGSI EFENDI PIPIH SUNINGSIH EFENDI PIPIH SUNINGSIH EFFENDI Prakoso, Nurul Muhammad Primasari Pertiwi PUSPITA LISDIYANTI Putra Mahanaim Tampubolon Rabbil Pratama Aji Rahayu, Ernawati Puji Rahma Suryaningsih Randall C Kyes RETNO LESTARI RETNO LESTARI Retno Lestari Retno Lestari Retno Lestari RETNO LESTARI Retno Lestari Riska S. Wahyuningtyas Rita Rostika RONI RIDWAN RUDHY GUSTIANO Rudi Afnan RUGAYAH RUGAYAH RURY EPRILURAHMAN Sabar Pambudi Sabbathini, Gabriela Christy Sanya Khaerunnisa Sawung Cindelaras, Sawung SHANNI FERNANDA Shanti Ratnakomala Siswantining, Titin Siti Z Musthofa SUCI LESTARI Sulistiani ., Sulistiani Sulistiani Sulistiani Sulistiani Sulistiani, Sulistiani Sunarma , Ade Sundari, Ayu Mulia Taniawati Supali Tito Latif Indra TRI ZULISTIANA Tri Aprilliana Wulandari Trianti, Lely Upi Chairun Nisa Uswatun Hasanah Uswatun Hasanah UTAMA ANDI Valerry Athalia Priyanka Wibowo Mangunwardoyo WIBOWO MANGUNWARDOYO WISNU WARDHANA Wulandari, Sri Rezeki Wulandhary, Sendy Yasman Yasman Yasman Yushinta Fujaya Zefanya Cellina Aurawicona Zuliyanto Zakaria ‪Melisnawati H. Angio