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Short Communication: Ethnozoology – The perception of Tobelo Dalam tribal community in Saolat Village, East Halmahera, Indonesia towards cuscus (Phalangeridae) ELIZABETH NOVI KUSUMANINGRUM; JATNA SUPRIATNA; ABINAWANTO ABINAWANTO; ANOM BOWOLAKSONO
Biodiversitas Journal of Biological Diversity Vol. 19 No. 6 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190621

Abstract

Kusumaningrum EN, Supriatna J, Abinawanto, Bowolaksono A. 2018. Short Communication: Ethnozoology – The perception of Tobelo Dalam tribal community in Saolat Village, East Halmahera, Indonesia towards cuscus (Phalangeridae). Biodiversitas 19: 2140-2146. The complexity of the hard lives of Tobelo Dalam tribal community who live in Saolat village, district South Wasile provokes the high activity of animal poaching in Aketajawe-Lolobata National Park (TNAL) including cuscus hunting for both sale and consumption purposes. The study aimed to describe the traditional knowledge of Tobelo Dalam people in Saolat village in applying the systems of utilization and local conservation knowledge towards cuscus. This study was analyzed by the qualitative method with explorative survey technique, interview, and completing questionnaires. This study used 4 key informants (3 males, 1 female) and 50 adult respondents (25 males and 25 females). The results showed that the average percentage of traditional knowledge owned by males and females were 69.1% and 30.9 %, respectively, so the males had an advantage of knowledge around 38.3 % than females in addressing the traditional knowledge of cuscus. The community of Tobelo Dalam tribe hunts cuscus for four different purposes, i.e., for traditional ceremonies, traditional medicine, cuisine, and sell it for more income to support the economy of family.
Genetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia INA ERLINAWATI; ABINAWANTO ABINAWANTO; ANDI SALAMAH; RUGAYAH RUGAYAH
Biodiversitas Journal of Biological Diversity Vol. 19 No. 6 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190648

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Erlinawati I, Abinawanto, Salamah A, Rugayah. 2018. Genetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia. Biodiversitas 19: 2374-2380. Daluga or the giant swamp taro (Cyrtosperma merkusii (Hassk.) Schott, a member of Araceae, is one of the minor tuber crops in Indonesia. It has high nutritional value for alternative food. Sequences-Related Amplified Polymorphism (SRAP) primers combinations were used for the first time to assess the genetic diversity among 36 of daluga accessions from several locations in Siau, Sangihe and Talaud islands, North Sulawesi, Indonesia. All of the samples were successfully extracted from silica-gel dried leaves using CTAB methods with minor modifications. Using six SRAP primer combinations, a total of 80 DNA fragments were yielded, varied from 75-1500 bp. The scoring of the fragments resulted in 68 (85.03%) polymorphic bands. Amongst four populations studied, Siau Island population has the highest level of genetic variation with mean values of Na = 1.9375±0.2436, Ne = 1.6409 ± 0.3255, PPL = 93.75%, H = 0.3614 ± 0.1516 and I = 0.5299 ± 0.1978. Meanwhile, Salibabu, Talaud Island population has the lowest level of genetic variation with mean values of Na = 1.5125 ± 0.5030, Ne = 1.3075 ± 0.3723 , PPL = 51.25%, H = 0.1800 ± 0.1989 and I = 0.2700 ± 0.2855. The high of polymorphic bands were generated by SRAP markers suitable for further analysis method in applied and basic of the genetic study on Cyrtosperma species and also related genera. This new genetic information can be used for baseline data for further research especially to improving the variation of daluga and for the conservation of daluga in the future.
DNA barcoding reveals underestimated species diversity of mantis shrimp larvae (stomatopods) in Banten Bay, Indonesia ABINAWANTO ABINAWANTO; MARIANA D. BAYU INTAN; WISNU WARDHANA; ANOM BOWOLAKSONO
Biodiversitas Journal of Biological Diversity Vol. 20 No. 6 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200637

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Abstract. Abinawanto, Intan MDB, Wardhana W, Bowolaksono A. 2019. DNA barcoding reveals species diversity of mantis shrimp larvae (stomatopods) from Banten Bay, Indonesia. Biodiversitas 20: 1758-1763. Larvae of stomatopods can be morphologically identified by linking between larval and adult form, but it's difficult applied in the laboratory because of requirement of condition to rearing larvae collected either from plankton, or spawning adults in the laboratory. Molecular methods are the alternative approach to gather accurate taxonomic identification. One of molecular methods is DNA barcoding based upon sequence diversity in cytochrome c oxidase subunit 1 (COI). Research on stomatopod larvae diversity and distribution at six stations in Banten Bay has been studied from October 2013 to March 2014. This study was conducted to identify the diversity of stomatopod larvae through DNA barcoding and also to examine the distribution of stomatopod larvae in Banten Bay. DNA barcoding was used to identify mantis shrimp larvae in Banten Bay, Indonesia. Partial mitochondrial COI were obtained for 37 larvae, revealing five distinct molecular operational taxonomic units (MOTUs). All MOTUs distinctively fell into Squillidea clade. Two Molecular Operational Taxonomic Units, MOTUs were successfully identified to species level (i.e. Anchisquilla fasciata), while three others remained identified to genus level (i.e. Stomatopoda spp). The intraspecific variation of COI ranged from 0% to 3.1% (Kimura 2-parameter distance), whereas interspecific divergence ranged from 5.9% to 20.4%. These results imply that COI genes are suitable for species identification of stomatopod larvae in this area, though the number of unidentified species was higher than the identified ones. This is likely because of the limited database in GenBank. In addition, the two identified species represents new records for this area, suggesting that the mantis shrimp in the area have been underestimated based on traditional biodiversity measures. We recommend to develop a taxonomically-comprehensive DNA barcoding database to improve the accuracy and feasibility of using DNA barcoding for species identification of Indonesia mantis shrimps.
Isolation and partial characterization of alginate extracted from Sargassum polycystum collected from three habitats in Banten, Indonesia NIKEN DHARMAYANTI; JATNA SUPRIATNA; ABINAWANTO ABINAWANTO; YASMAN YASMAN
Biodiversitas Journal of Biological Diversity Vol. 20 No. 6 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200640

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Abstract. Dharmayanti N, Supriatna J, Abinawanto, Yasman. 2019. Isolation and partial characterization of Alginate from Sargassum polycystum of Banten, Indonesia. Biodiversitas 20: 1776-1785. The utilization of Sargassum polycystum as an alternative alginate source may reduce the dependence on alginate import as currently alginate demands in Indonesia are 100% supplied from overseas. The purpose of this study was to characterize alginate extracted from Sargassum polycystum obtained from three locations with different ecological characteristics. Isolation of alginate was conducted through partial hydrolysis to separate guluronic acid and mannuronic acid followed by freeze-drying and then the parameters were measured qualitatively and quantitatively using FTIR. The results showed that alginate extracted from Sargassum polycystum collected from Lima Island, Ujung Kulon and Binuangeun were 11.48%, 18.62%, and 5.75%, respectively with the viscosity were 35 cP, 62.50 cP, and 81.33 cP, respectively. The test result of partial hydrolysis of alginate showed that guluronic block (GG) in the alginate polymer of Lima Island, Ujung Kulon and Binuangeun were 67.60%, 59.00%, and 41.40%, respectively. These relate to the nature of the gel formed. The alginate from Lima Island tends to be more rigid and less flexible than that from Ujung Kulon and Binuangeun. The findings of this study suggest that there are differences in the concentration of the components of mannuronate and guluronate of Sargassum polycystum across different locations in Banten, Indonesia.
Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics ABINAWANTO ABINAWANTO; ALFI SOPHIAN; RETNO LESTARI; ANOM BOWOLAKSONO; PIPIH SUNINGSIH EFENDI; RUDI AFNAN
Biodiversitas Journal of Biological Diversity Vol. 20 No. 7 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200729

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Abstract. Abinawanto, Sophian A, Lestari R, Bowolaksono A, Efendi PS, Afnan R. 2019. Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics. Biodiversitas 20: 2004-2010. Insulin-like growth factor 1 (IGF-1) is defined as an essential gene for growth. Therefore, this research aims to identify the polymorphism of the IGF-1 gene from ayam ketawa with the dangdut and slow type vocal characteristics. Twenty male chickens were selected as an animal model. The sample consisted of 10 chickens with the dangdut type and 10 chickens with the slow type. The chickens were all obtained from five areas of the Pinrang district. The RFLP and HRM methods were selected to be used in this research. Results showed that the IGF-1 gene was 622 bp in length, while polymorphism analysis showed that the dangdut and slow type are homozygotic. The unrestricted DNA fragment indicated it after reaction of the restriction enzyme Pst1. Moreover, it confirmed that ayam ketawa with the dangdut or slow type had a similar sequence.
The detection of plantaricin-encoding genes and their amino acid profiles in Lactobacillus plantarum AKK30 isolated from Indonesian native chicken ELISABETH LOVIAN ULI BASA; Abinawanto Abinawanto; A. SOPHIAN; H. JULENDRA; A. SOFYAN
Biodiversitas Journal of Biological Diversity Vol. 21 No. 12 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d211241

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Abstract. Elisabeth LUB, Abinawanto, Sophian A, Julendra H, Sofyan A. 2020. The detection of plantaricin-encoding genes and amino acid profile in Lactobacillus plantarum AKK30 isolated from Indonesian native chicken. Biodiversitas 21: 5792-5799. Plantaricin-encoding genes of lactic acid bacteria (LAB) Lactobacillus plantarum AKK30 which was isolated from Indonesian native chicken has been characterized using DNA sequencing technology. In order to substitute antibiotics for chickens and enhance probiotics in their gut, characterization of the indigenous probiotics in the chicken should be conducted. A potential probiotic in native chicken gut is Lactobacillus plantarum AKK30. Genes that harbor the bacteriocin biosynthesis of the strain were reported for the first time. The bacterium has diverse plantaricin genes, i.e., plnA, plnEF, plnJ, plnK and plnO. According to the dendrogram, the five plantaricin genes were closely related to plnA, plnE, plnF, plnJ, plnK and plnO genes from L. plantarum WCFS1, L. plantarum C11, and L. plantarum V90. Intriguingly, besides various plantaricin-encoding genes, another important probiotic property in L. plantarum AKK30 is cholesterol-lowering activity. Amino acid profiling also indicated proteolysis activity and probiotic potential, for instance, GABA production. Thus, L. plantarum AKK30 may be added in chicken broiler’s diet to provide probiotic benefits.
Short Communication: Effect of cryopreservation on ultrastructure and mitochondrial function of albino Pangasius catfish spermatozoa Uswatun Hasanah; ABINAWANTO ABINAWANTO; A ALIMUDDIN; Arief Boediono; Eni Kusrini
Biodiversitas Journal of Biological Diversity Vol. 21 No. 10 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d211008

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Abstract. Hasanah U, Binawanto, Alimuddin A, Boediono A, Kursini E. 2020. Short Communication: Effect of cryopreservation on ultrastructure and mitochondrial function of albino Pangasius catfish spermatozoa. Biodiversitas 21: 4524-4528. Cryopreservation techniques have been carried out on many endangered species and animals with unique characteristics. Successful cryopreservation techniques vary between species depending on various factors. The study used cryopreserved spermatozoa of the albino Pangasius catfish as samples. The cryopreserved spermatozoa were analyzed by its ultrastructure, functional mitochondria, and viability. The cryopreservation was performed using a combination of 10% methanol, skim milk, and fish ringer extender. A deep freezer is used for cryopreservation at -80 °C with a storage period of 14 days. Observations were made on fresh spermatozoa, post-equilibration spermatozoa, and frozen-thaw spermatozoa. This study found there were differences in ultrastructure and morphology in the three treatments. Fresh spermatozoa and post-equilibration spermatozoa appeared intact membrane, mitochondrial, and flagellar structures. In contrast, in frozen-thaw spermatozoa, there was damage to the cell membrane. The study showed different percentages of yields on functional mitochondria of fresh spermatozoa (98 ± 2%), spermatozoa post-equilibration (57 ± 7%), frozen-thaw spermatozoa (42 ± 3.21%). Cell viability showed that there were differences in viability of fresh spermatozoa and frozen-thaw (p <0.05), the results of fresh spermatozoa (92 ± 0.57%) spermatozoa post-equilibration (80 ± 3.51%), frozen-thaw spermatozoa (61 ± 2.30%).The study concluded that the spermatozoa cryopreservation affects the ultrastructure, mitochondrial function, and viability in albino Pangasius catfish spermatozoa.
The genetic diversity of ayam ketawa (Gallus gallus domesticus, Linneaus, 1758) in Bangkalan District, Madura Island, Indonesia ABINAWANTO Abinawanto; TRI ZULISTIANA; RETNO LESTARI; ASTARI DWIRANTI; ANOM BOWOLAKSONO
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220617

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Abstract. Abinawanto, Zulistiana T, Lestari R, Dwiranti A, Bowolaksono A. 2021. The genetic diversity of ayam ketawa (Gallus gallus domesticus, Linneaus, 1758) in Bangkalan District, Madura Island, Indonesia. Biodiversitas 22: 3145-3155. Ayam Ketawa, a variation of Gallus gallus domesticus Gallus gallus domesticus, comes from  Sidendreng-Rappang (Sidrap) District, South Sulawesi, Indonesia. Apart from Sidrap, ayam ketawa farms are also found in Bangkalan District, on the island of Madura, Indonesia. Ayam ketawa from Bangkalan District often wins national level contests. Therefore, the research was carried out with the aim of knowing the genetic diversity of ayam ketawa in Kamal Subdistrict, Socah Subdistrict, and Burneh Subdistrict, based on bioacoustic, morphometric, and barcoding DNA analysis. The studied ayam ketawa consists of dangdut and slow types. The longest duration (6.00 ± 3.0) seconds was shown by ayam ketawa from Kamal Subdistrict, while the highest number of syllables (7.20 ± 5.80) came from Socah Subdistrict. The results of the Mann-Whitney test showed that eight morphological characters were significantly different (P <0.05) between ayam ketawa population in Kamal and Burneh subdistricts, namely body weight, femur length, shank length, shank circumference, wing length, comb height, third finger length, and breastbone length. Four morphological characters differed significantly (P <0.05) between ayam ketawa population in Kamal and Socah subdistricts, namely shank length, wing length, third finger length, and sternum length. Three morphological characters were significantly different (P <0.05) between the Burneh and Socah subdistricts population, namely body weight, femur length, and shank circumference. The analysis results of DNA Barcoding gene of the Cytochrome Oxidase subunit 1 (CO1) showed a genetic distance ranging from 0.025 to 1.872.
Morphometric analysis of Gorontalo (Indonesia) native chickens from six different regions ALFI SOPHIAN; ABINAWANTO ABINAWANTO; UPI CHAIRUN NISA; FADHILLAH FADHILLAH
Biodiversitas Journal of Biological Diversity Vol. 22 No. 4 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220420

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Abstract. Sophian A, Abinawanto, Nisa UC, Fadhillah. 2021. Morphometric analysis of Gorontalo (Indonesia) native chickens from six different regions. Biodiversitas 22: 1757-1763. Research on the diversity of Gorontalo native chickens based on morphometric analysis of body weight, leg length, head length, head length to beak and head width was conducted. This study was conducted to identify the diversity of native chickens in six regions spread across Gorontalo Province, Indonesia. The method used in conducting statistical analysis is the principal component analysis (PCA) method. This method was performed to identify which morphometric factors play a role in relationship analysis of Gorontalo native chickens. Based on statistical analysis, it was found that of the 5 variables used in this study, 3 variables had Measure of Sampling Adequacy (MSA) values > 0.5 ranged from 0.537 to 0.648, namely body weight, leg length, and head length. Meanwhile, the other 2 variables, namely Head to Beak Length and Head Width, have MSA values <0.5 with values of 0.313 and 0.316. This causes the other 2 variables to be used as a differentiating variable in analyzing the relationship between native chickens from six different regions scattered in the Gorontalo Province. The morphometric analysis of body weight means that the chickens sampled from Boalemo were morphometrically the largest in size, while the chickens sampled from Pohuwato were morphometrically the smallest. Chickens sampled from North Gorontalo, Bone Bolango, and Gorontalo City tended to be more uniform when compared to the chickens sampled from Pohuwato and Boalemo areas, while for Gorontalo District it was larger in size than Pohuwato, North Gorontalo, Bone Bolango, and Gorontalo City, but smaller than Boalemo.
Genetic diversity analysis and phylogenetic reconstruction of groupers Epinephelus spp. from Madura Island, Indonesia based on partial sequence of CO1 gene ABDUL BASITH; Abinawanto Abinawanto; ENI KUSRINI; YASMAN YASMAN
Biodiversitas Journal of Biological Diversity Vol. 22 No. 10 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d221020

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Abstract. Basith A, Abinawanto, Kusrini E, Yasman. 2021. Genetic diversity analysis and phylogenetic reconstruction of groupers Epinephelus spp. from Madura Island, Indonesia based on partial sequence of CO1 gene. Biodiversitas 22: 4282-4290. Groupers populations in Indonesia, particularly from Madura Island, East Java are indicated to be over-fished, thereby requiring data collection of more accurate genetic resources as an important step for grouper conservation. A total of 14 samples of the Epinepheplus groupers were obtained from the fish landing port on Madura Island. The 617 bp CO1 gene sequence was utilized for genetic diversity analysis and phylogenetic tree reconstruction. Genetic diversity is based on the value of haplotype diversity (Hd) and nucleotide diversity (?). Reconstruction of the phylogenetic tree includes neighbor-joining (NJ) implementing K2P substitution model, while maximum likelihood (ML) is conducted by implementing HKY+G+I substitution model, both of which were evaluated by employing a bootstrap of 1000 replications. Analysis of genetic distance between species indicated that the farthest distance between E. heniochus and E. fasciatus was 0.189, while the closest distance between E. erythrurus and E. ongus was 0.099. Intrapopulation genetic diversity indicated a high value with details of Hd=0.978 and ?=0.12107. Furthermore, NJ and ML phylogenetic tree demonstrated similar topology in the observed Epinephelus spp. obtained from Madura Island grouped into 7 clades, that is Epinephelus coioides, E. bleekeri, E. areolatus, E. erythrurus, E. heniochus, E. fasciatus, and E. ongus.
Co-Authors A. SOFYAN A. Sofyan A. Sofyan A. SOPHIAN ABDUL BASITH Abdul Basith Achmad Dinoto Achmad Dinoto Achnafani, Dini Adam Heray Hutabarat Ade Sunarma Advent Tri Joy Simamora Ady Suryawan Afifa Hasna Agung Sudaryono Agus Haryadi Ahmad Aulia Jusuf ALFI SOPHIAN ALFI SOPHIAN ALFI SOPHIAN Alimuddin ANANG HARI KRISTANTO Andi Salamah Aniqah, Sunni Sofiah Anita Mayasari ANOM BOWOLAKSONO Arief Boediono Arief Boediono ASRI SULFIANTI Astari Dwiranti Ayu Mulia Sundari Ayu Mulia Sundari Azzizah, Isma Nur Bayu , Mariana D. Bintang Mahakarya Sembahen Cendana, Kartika Sari Dewi Susiloningtyas Diki Nurul Huda Djamhuriyah S. Said DODDY IRAWAN SETYO UTOMO EKA DEWI SRIYANI ELISABETH LOVIAN ULI BASA ELIZABETH NOVI KUSUMANINGRUM Endang C. Purba Endang Sukara ENI KUSRINI Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini, Eni Erma Primanita Hayuningtyas Fadhilah FADHILLAH FADHILLAH Farisi, Salman Fitrianingrum, Nisa Funahashi, Hiroaki H. JULENDRA H. Julendra HANI HAMIDAH Hidayat Hidayat Hiroaki Funahashi Huriyah Adani Saoemi INA ERLINAWATI IS HELIANTI is helianti IS HELIANTI Ita M. Nainggolan Jatna Supriatna L. Istiqomah Lina Mulyawati LULUT DWI SULISTYANINGSIH Lulut Dwi Sulistyaningsih LUTHFIRALDA SJAHFIRDI M. F. Karimy M. F. Karimy Maria Ulfah Maria Ulfah Mariana D. Bayu MARIANA D. BAYU INTAN Marina Silalahi MARLINA ARDIYANI Marlina Ardiyani Maylasari, Roospita MILANI ANGGIANI Nataniel, Jocelyn NIKEN DHARMAYANTI NIKNIK NURHAYATI NIKNIK NURHAYATI Nisyawati Nova Anita Noviyanti, Rintis Nurfitriani, Risya Nurul Muhammad Prakoso Odang Carman PIPIH SUNINGSI EFENDI PIPIH SUNINGSIH EFENDI PIPIH SUNINGSIH EFFENDI Prakoso, Nurul Muhammad Primasari Pertiwi PUSPITA LISDIYANTI Putra Mahanaim Tampubolon Rabbil Pratama Aji Rahayu, Ernawati Puji Rahma Suryaningsih Randall C Kyes RETNO LESTARI RETNO LESTARI Retno Lestari Retno Lestari Retno Lestari RETNO LESTARI Retno Lestari Riska S. Wahyuningtyas Rita Rostika RONI RIDWAN RUDHY GUSTIANO Rudi Afnan RUGAYAH RUGAYAH RURY EPRILURAHMAN Sabar Pambudi Sabbathini, Gabriela Christy Sanya Khaerunnisa Sawung Cindelaras, Sawung SHANNI FERNANDA Shanti Ratnakomala Siswantining, Titin Siti Z Musthofa SUCI LESTARI Sulistiani ., Sulistiani Sulistiani Sulistiani Sulistiani Sulistiani, Sulistiani Sunarma , Ade Sundari, Ayu Mulia Taniawati Supali Tito Latif Indra TRI ZULISTIANA Tri Aprilliana Wulandari Trianti, Lely Upi Chairun Nisa Uswatun Hasanah Uswatun Hasanah UTAMA ANDI Valerry Athalia Priyanka Wibowo Mangunwardoyo WIBOWO MANGUNWARDOYO WISNU WARDHANA Wulandari, Sri Rezeki Wulandhary, Sendy Yasman Yasman Yasman Yushinta Fujaya Zefanya Cellina Aurawicona Zuliyanto Zakaria ‪Melisnawati H. Angio