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All Journal HAYATI Journal of Biosciences International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana Bumi Lestari ISM (Intisari Sains Medis) : Jurnal Kedokteran
Ni Putu Adriani Astiti
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Medicine & Pharmacology

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ANALISIS KANDUNGAN FENOLIK EKSTRAK DAUN JATI (Tectona grandis L.) DENGAN WAKTU DEKOMPOSISI YANG BERBEDA Ni Putu Adriani Astiti
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p18

Abstract

Teak (Tectona grandis L.) is a plant with strong wood and is resistant to pests and diseases. In addition, its leaf extract is widely used by public as an additive in foods both as a preservative and as an alternative food coloring. The objective of this research was to determine phenolic compounds of teak leaves extracted with three different decomposition times (one, two and three weeks of decomposition). Extraction was conducted by using maceration method in which water was used as solvent. Identification of Phenolics was done by using Gas Chromatography. The results showed that the compounds of vanilic acid, salicylic acid, ferulic acid, cumaric acid, gallic acid, benzoic acid and cafeic acid were identified in the analysis. The concentration and composition of phenolic compounds in the extract was determined by decomposition.
PEMBERIAN KOLKHISIN DENGAN LAMA PERENDAMAN BERBEDA PADA INDUKSI POLIPLOIDI TANAMAN PACAR AIR (IMPATIENS BALSAMINA L.) Ni Made Sastriyani Wiendra; Made Pharmawati; Ni Putu Adriani Astiti
Jurnal Biologi Udayana Vol 15 No 1 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The purpose of this research was to induce polyploidy on Impatiens balsamina L. Seedlings of I. balsamina was immersed in 0,01% colchicines solution for 4, 6, 8, and 24 hours. Chromosome of I. balsamina was visualized using squash method stained with aceto-orcein. Morphological observations were conducted on plant height, stem circumference, leaf length and width, number of branches flowering time and size of flower. The result revealed that 0,01% solution of colchicine was able to induce polyploidy on I. balsamina. Obsevation on morphological characteristic showed that colchicine treatment increased plant height, stem circumference, leaf length and number of branches. Colchicine treatment induced earlier flowering time, but flower size was unaffected. Twelve chromosomes (2n = 2x = 12) were observed in the diploid seedlings while 25 chromosomes were observed in the tetraploid (2n = 4x = 24 seedlings.
KANDUNGAN FITOKIMIA EKSTRAK DAUN KAMBOJA (Plumeria sp.) DAN PENGARUHNYA TERHADAP PERTUMBUHAN TANAMAN JAHE EMPRIT (Zingiber officinale var. Amarum) Putu Yayun Antari Budaya; Ni Putu Adriani Astiti; Eniek Kriswiyanti
Jurnal Biologi Udayana Vol 19 No 1 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Tanaman kamboja mengandung senyawa alelopati yang menghambat pertumbuhan tanaman di sekitarnya.  Penelitian ini bertujuan untuk mengetahui kandungan fitokimia ekstrak daun kamboja dan pengaruhnya terhadap pertumbuhan tanaman jahe emprit (Zingiber officinale var. amarum). Penelitian dilaksanakan dalam dua tahap yaitu, tahap pertama uji fitokimia ekstrak daun kamboja merah dan daun kamboja putih, serta tahap kedua berupa uji respon pertumbuhan tanaman jahe emprit yang diberi perlakuan ekstrak daun kamboja. Variabel respon pertumbuhan yang diamati adalah jumlah tunas tumbuh, tinggi tanaman, jumlah daun, jumlah akar, berat rimpang, berat basah berangkasan, dan berat kering berangkasan. Hasil uji fitokimia menunjukkan bahwa pada ekstrak daun kamboja putih dan kamboja merah memiliki jenis golongan senyawa aktif yang sama yaitu triterpenoid, steroid, flavonoid, dan polifenol dalam intensitas yang berbeda. Ekstrak daun kamboja putih memberikan efekdaya hambat yang lebih kuat dibandingkan dengan ekstrak daun kamboja merah. Persentase daya hambat ekstrak daun kamboja putih terhadap tinggi tanaman jahe emprit sebesar 80,5%, berat basah berangkasan 64%, jumlah daun 54%, berat kering berangkasan 54%, jumlah tunas tumbuh 33,3%, dan berat rimpang 31,9%.
UJI AKTIVITAS EKSTRAK REBUNG BAMBU TABAH (Gigantochloa nigrociliata BUSE-KURZ) TERHADAP PERILAKU KAWIN MENCIT JANTAN (Mus musculus L.) A. A. Istri Mas Padmiswari; A. A. S. A Sukmaningsih K; Ni Putu Adriani Astiti
Jurnal Biologi Udayana Vol 19 No 1 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitan ini bertujuan untuk mengetahui pengaruh ekstrak rebung bambu tabah terhadap perilaku kawin mencit jantan. Rancangan yang digunakan pada penelitian ini adalah rancangan eksperimental sederhana dengan4 kelompok perlakuan dan 6 ulangan, yaitu P0: Kontrol (Perlakuan  dengan pemberian CMC Na 0,5 %), P1: (Perlakuan dengan pemberian ekstrak 200 mg/kg bb), P2: (Perlakuan dengan pemberian ekstrak 300 mg/kg bb) dan P3: (Perlakuan dengan pemberian ekstrak 400 mg/kg). Perlakuan ekstrak rebung bambu tabah diberikan pada mencit jantan secara oral setiap hari selama 33 hari dan pengamatan perilaku kawin dilakukan 3 hari sekali. Variabel yang diamati untuk menilai perilaku kawin mencit jantan adalah jumlah mount. Data dianalisa dengan menggunakan uji One Way Annova. Hasil penelitian menunjukkan bahwa pemberian ekstrak rebung bambu tabah berpengaruh signifikan terhadap peningkatan perilaku kawin mencit jantan (P<0,05).
DAYA HAMBAT EKSTRAK DAUN RAMBUTAN RAPIAH (Nephelium lappaceum L.) TERHADAP PERTUMBUHAN TANAMAN TEMULAWAK A. A. Istri Mirah Dharmadewi; Ni Putu Adriani Astiti; Luh Putu Wrasiati
Jurnal Biologi Udayana Vol 18 No 2 (2014): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Rambutan rapiah (Nephelium lappaceum L.) merupakan salah satu tanaman yang dapat digunakan sebagai obat yang termasuk kedalam family Sapindaceae. Rambutan mengandung senyawa alelopati yang dapat menghambat pertumbuhan tanaman yang tumbuh disekitarnya. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak daun rambutan rapiah terhadap pertumbuhan temulawak serta mengetahui kemampuan tanaman temulawak untuk bertahan terhadap pemberian ekstrak daun rambutan. Parameter yang diamati adalah tinggi tanaman, panjang daun, lebar daun, jumlah daun, jumlah tunas dan berat rimpang yang diamati pada akhir pengamatan. Pemberian ekstrak daun rambutan dengan konsentrasi 5%,10%,15%, 20% mengakibatkan tinggi tanaman, panjang daun, lebardaun, jumlah daun dan berat rimpang lebih rendah jika dibandingkan dengan kontrol.
JENIS, STATUS DAN PEMANFAATAN TUMBUHAN JENIS POHON DI DESA ADAT BATURNING, KECAMATAN ABIANSEMAL, KABUPATEN BADUNG, BALI N. P. Adriani Astiti; Retno Kawuri; I K. Ginantra
Bumi Lestari Journal of Environment Vol 8 No 2 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

There were 65 species of trees have been identified in Baturning village, District of Abiansemal, Regency of Badung, Bali, which were spread out around the river bank, cemetery (around Dalem temple), around the settlement, non-irrigated farm (plantation or “tegalan”) and rice field. Thirty eight (38) % out of them were observed to be available around the settlement. Most of trees in Baturning (61 species or 94%) have been used by the local community. The rest were uncultivated. From the use, trees in Baturning most were used for human consumption (24%). On the village has not been found protected species, even though in the village were found 22 species of trees which considered to be rare in Bali, namely: Bayur, Bentenung, Beringin, Cempaka, Ee, Jaka, Jeruk Bali, Juwet, Kelor, Kendal, Klampuak, Lempeni, Lenggung, Mahoni, Mengkudu, Palem Raja, Poh-Pohan, Pule, Sandat, Sentul, Udu, and Wani.
EFEKTIVITAS EKSTRAK DAUN JATI ( TECTONA GRANDIS L. F) DALAM MENGHAMBAT PERTUMBUHAN JAMUR HORMISCIUM Sp. Ni Putu Adriani Astiti
Bumi Lestari Journal of Environment Vol 15 No 1 (2015)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A research on effectiveness of extracts of teak (Tectona grandis L. f) leaves in inhibit thegrowth of a species of fungi, Hormiscium sp. has been carried out.  The leaves used in thisresearch were young leaves (number 1 and 2), and old ones (number 5 and 6), with fourconcentrations: 0, 2, 5, and 10 %.  The media utilised were bean sprouts agar, and thereplications was 3 times.  Qualitative tests were undertaken for the flavonoids, while alkaloidtests were based on Culvenor Fitzgerald methods.  Qualitative tests of triterpenoids andsteroids were undertaken by utilising Lieberman – Burchard reagents (L-B reagents).Parameters observed were colony diameters, micellium weights (dry mass), and numbers ofconidia (ml).  Results of this research showed that extracts of teak (T. grandis) leaves wereable to inhibit the growth of Hormiscium sp.  The capability of inhibitions was increased asthe extract concentrations increased (for both, that of the young and old leaves), eventhough extracts of the young provided a higher capability of inhibitions compared to theold ones.
Efektifitas daun salam dalam mengurangi cemaran mikroba penyebab busuk telur itik Yulidia Iriani; Yan Ramona; Ni Putu Adriani Astiti
Intisari Sains Medis Vol. 13 No. 2 (2022): (In Press : 1 August 2022)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (712.767 KB) | DOI: 10.15562/ism.v13i2.1321

Abstract

Background: Pathogenic bacteria on eggshells or egg contents will be very dangerous for the health of humans who consume them. Chemicals should not be an option for preservatives because they will cause side effects. In this study, we explored the possibility of using bay leaf extract (Syzygium polyanthum (Wight) Walp) to control the growth of polluting bacteria in salted duck eggs so that the shelf life can be extended. The purpose of the study was to determine the potential of the active compounds contained in bay leaves in reducing the total plate count/microbial contamination in salted duck eggs. Methods: Extraction of bay leaf powder was carried out by the infusion method. The activity test of bay leaf decoction (ARDS) was carried out using the in vitro diffusion well method on Mueller Hinton media. Identification of active compounds was carried out using the GCMS technique. Results: ARDS crude extract at a concentration of 5% (v/v) could significantly reduce ALT and inhibit the in vitro growth of Proteus mirabilis bacteria at a concentration of 10% (w/v), with an inhibition zone diameter of 19.53 mm, with the minimum value inhibitory concentration (MIC) 0.7%. The results of the GCMS analysis of ARDS showed that the ten peaks produced, possibly synergized, and had antimicrobial activity. These compounds are 2-Pentanone4-Hydroxy-4 Methyl (CAS) Diacetone Alcohol; Ethanol, 2-(Ethoxythoxy) (CAS); 1,2,3 Propanetrriol Cas) Glycerol; Citronella (2,6-Octadien-1-Ol, 3,7-Dimethyl, (CAS); Beta Citronellol 6-Octen-1-Ol, 3,7 Dimethyl-(CAS); Trans Geraniol; Dodecanoic Acid, Methyl Ester (CAS) Methyl Laurate; Tetradecanoic Acid, Methyl Ester (CAS) Methyl Myristate; 1-Tetradecanol (Cas) Alfol 14 and Hexadecanoic Acid Methyl Ester CAS) Methyl Palmitate Conclusion: Leaf decoction can inhibit the growth of Proteus mirabilis in vitro, and the addition of ARDS with a concentration of 5% (v/v) can significantly reduce the value of bacterial contamination in salted duck eggs.   Latar Belakang: Bakteri pathogen pada kulit telur atau isi telur akan sangat membahayakan kesehatan manusia yang mengkonsumsinya. Bahan kimia seharusnya tidak menjadi pilihan untuk pengawet, karena akan menimbulkan efek samping, Pada penelitian ini dieksplorasi, kemungkinan dipakainya ekstrak daun salam (Syzygium polyanthum (Wight) Walp) untuk mengontrol pertumbuhan bakteri pencemar pada telur itik yang diasinkan, sehingga masa simpannya dapat diperpanjang. Tujuan penelitian untuk mengetahui potensi senyawa aktif yang terdapat pada daun salam dalam menurunkan angka lempeng total/cemaran mikroba pada telur itik yang diasinkan. Metode: Ekstraksi serbuk daun salam dilakukan dengan metode infusa. Uji aktifitas air rebusan daun salam (ARDS) dilakukan dengan metode sumur difusi secara in vitro pada media Mueller Hinton. Identifikasi senyawa aktif dilakukan dengan teknik GCMS. Hasil: Ekstrak kasar ARDS pada konsentrasi 5% (v/v) secara nyata dapat menurunkan ALT dan menghambat pertumbuhan in vitro bakteri Proteus mirabilis pada konsentrasi 10% (b/v), dengan diameter zona hambat sebesar 19,53 mm, nilai minimum inhibitory concentration (MIC) 0,7%. Hasil analisis GCMS terhadap ARDS menunjukan bahwa sepuluh puncak yang dihasilkan, kemungkinan bersinergi dan mempunyai aktifitas sebagai antimikroba. Senyawa-senyawa tersebut adalah 2-Pentanone4-Hydroxi-4 Methyl (CAS) Diacetone Alkohol; Ethanol, 2-(Ethoxythoxy) (CAS); 1,2,3 Propanetrriol Cas) Glycerol; Citronella (2,6-Octadien-1-Ol, 3,7-Dimethyl, (CAS); Beta Citronellol 6-Octen-1-Ol, 3,7 Dimethyl-(CAS); Trans Geraniol; Dodecanoic Acid, Methyl Ester(CAS) Methyl Laurate; Tetradecanoic Acid,Methyl Ester (CAS) Methyl Myristate; 1-Tetradecanol (Cas) Alfol 14 dan Hexadecanoic Acid Methyl Ester CAS) Methyl Palmitate. Simpulan: Air rebusan daun dapat menghambat pertumbuhan Proteus mirablis secara in vitro dan penambahan ARDS dengan konsentrasi 5% (v/v) secara signifikan dapat menurunkan nilai cemaran bakteri (angka lempeng total) pada telur itik yang diasinkan.
Co-Authors A.A.S. A. Sukmaningsih AA Istri Mas Padmiswari Bernadina Metboki Deglory Tunmuni Dwi Ariani Yulihastuti ENIEK KRISWIYANTI I Gusti Ayu Sugi Wahyuni I Ketut Ginantra I Ketut Suada IDA AYU ASTARINI Ida Ayu Meva Lianawati Ida Ayu Nopiari Ida Bagus Gede Darmayasa IRIANI SETYAWATI Jeremy Pramana Kadek Ayu Trisna Yanti Lilis Riana Tambunan Luh Putu Wrasiati MADE PHARMAWATI Made Ria Defiani Meitini Proborini Wahyuni Ngurah Intan Wiratmini Ni Kade Ayu Purnama Adi Ni Komang Tia Pramesti Ni Luh Suriani Ni Made Dewi Wahyuni Ni Made Puspawati Ni Made Sastriyani Wiendra Ni Nyoman Nila Arieswari NI PUTU SRI PUSPITA WIDI YANTHI Ni Wayan Sudatri Pande Putu Purwaningsih Putu Yayun Antari Budaya Retno Kawuri Riana Dyah Suryaningrum Royana Pakpahan SANG KETUT SUDIRGA SARMILA TASNIM Yan Ramona Yuli Setiawati Yulidia Iriani Yulidia Iriani Yully Muharyati