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All Journal HAYATI Journal of Biosciences International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana Bumi Lestari SIMBIOSIS ISM (Intisari Sains Medis) : Jurnal Kedokteran Advances in Tropical Biodiversity and Environmental Sciences

Ni Putu Adriani Astiti

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 220296

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

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AKLIMATISASI ANGGREK HITAM (Coelogyne pandurata Lindl.) HASIL PERBANYAKAN IN VITRO PADA MEDIA BERBEDA Ni Kade Ayu Purnama Adi; Ida Ayu Astarini; Ni Putu Adriani Astiti
SIMBIOSIS Vol 2 No 2 (2014)
Publisher : Jurusan Biologi Universitas Udayana

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Black orchid (Coelogyne pandurata Lindl.) is an orchid endemic to the island ofBorneo. However, its existence is increasingly threatened with extinction. Conventionalpropagation efforts require a long time. Therefore in vitro propagation was performed. Thepurpose of this study was to determine the growth response of black orchids on the media anddifferent planting techniques. Black orchid plantlets that have been sub-cultured wasacclimatized in four different media types ie moss, fern, wood charcoal, wood charcoal andcoconut fiber mixture and different planting techniques namely compot (community pot) andthe individual, were allowed to grow for three months. Randomized block design (RBD) wasutilised, obtained 8 combination treatments, with 7 replicates. The results showed blackorchid has a good growth response in the media moss, ferns, and a mixture of wood charcoaland coconut fiber, while the wood charcoal media showed unfavorable results. Differentplanting techniques showed no significant results. Percentage of high life shown in mixedmedia wood charcoal and coconut fiber and compot techniques.Keyword : Black orchid (Coelogyne pandurata Lindl.), acclimatization, media

EFEKTIFITAS DAYA HAMBAT BAKTERI Streptomyces sp TERHADAP Erwinia sp PENYEBAB PENYAKIT BUSUK REBAH PADA TANAMAN LIDAH BUAYA (Aloe barbadensis Mill) SARMILA TASNIM; RETNO KAWURI; NI PUTU ADRIANI ASTITI
SIMBIOSIS Vol I, No 1, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

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Streptomyces sp was conducted from December 2010 - June 2011 at the Laboratoryof Microbiology, Biology Department, Math and Science Faculty, UdayanaUniversity Bukit Jimbaran-Bali. Implementation stages of the research consisted ofisolation and testing of the antibiotic activity Streptomyces sp to inhibit growthbacterial pathogens Erwinia sp as a cause of disease in plants fallen foul (Soft rot) ofAloe barbadensis Mill.The results of this study have eight isolates of Streptomyces spwith macroscopic and microscopic characters are varied. Furthermore, all isolateswere obtained and then tested against antibiotic activity to inhibit growth the bacteriaErwinia sp. Test results obtained by Streptomyces sp that has the most effective ininhibiting the ability of the bacteria Erwinia sp isolates are Streptomyces sp2for (45%).

IDENTIFIKASI SENYAWA AKTIF DAUN PLETEKAN (Ruellia tuberosa L.) DENGAN MENGGUNAKAN GC-MS Ida Ayu Nopiari; Ni Putu Adriani Astiti; Ngurah Intan Wiratmini
SIMBIOSIS Vol 4 No 2 (2016)
Publisher : Jurusan Biologi Universitas Udayana

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ABSTRACT The use of traditional medicines in human therapies has been claimed to be much safer than that of synthetic medicines with many negative side effects. Pletekan plant (Ruellia tuberosa L.) has been used empirically for years as an antidiabetic as its leaves contain poly phenolic compounds with the ability to stimulate beta cells of the pancreas so that its capability to produce insulin improved. The main objective of our research was to identify active compounds contained in Ruellia tuberosa L. leaves extracted with ethanol. Fractionation of these active compounds was conducted by applying column and thin layer chromatography using Dichlorometan and Ethylacetat with a ratio of 6:3 as eluent. Each active compound resulted from fractionation was identified using GC-MS analysis. The results showed that four active compounds (Hexadecanamide, 9-Octadecenamide, (Z), Octadecenamide and 1,2-Benzenedicarboxylic acid were identified in this analysis. Keywords: Ruellia tuberosa L., identification of active compounds.

EKSPLORASI SPATIAL DAN IDENTIFIKASI CENDAWAN ENDOFIT PADA TANAMAN KAKAO (Theobroma cacao L.) DI BALI Lilis Riana Tambunan; Meitini Proborini; Putu Adriani Astiti
SIMBIOSIS Vol 6 No 1 (2018)
Publisher : Jurusan Biologi Universitas Udayana

Cocoa plant (Theobroma cacao L.) can grow well in the highlands and lowlands, a plantation and industrial plantwhich is known as one of the export commodities, as raw material for the food and pharmaceutical industries which cancontribute to an increase in foreign exchange endophytic fungus is a fungus living on a live network of plants such asseeds, leaves, flowers, twigs, stems and roots. Truffles are generally mutualistic endophyte and is known to stimulate thegrowth of plants, capable of producing antibiotics and plant growth regulators (hormones). This study aims to determinethe diversity and identify endophytic fungi on Kakao.yang found in two different locations in Bali, in the village of KajaBuahan District of Payangan, Gianyar and the Village of Tua, Marga, Tabanan regency subdistricts. Research carried outin the field with the exploration methods, isolation, identification and diversity analysis conducted at the Laboratory ofPlant Taxonomy (Mycology), Department of Biology, Faculty of Mathematics, University of Udayana. Laboratoryresearch conducted by isolating the roots, leaves, plant stems and content of the soil (rhizosphere) cocoa. Once isolated,grown on PDA medium subsequently incubated at room temperature 250C. Isolates that grow back isolated and purifiedon a PDA medium for further identified to the genera or species level. A total of six isolates of endophytic fungi identifiedin the rhizosphere and cocoa plant that is where four of these isolates were identified to species, two isolates were notidentified until genus. Fungi were identified namely Aspergillus flavus, Rhizopus stolonifer, Cladosporiumsphaeropermum, Trichoderma viride, isolates 1 and 2 isolates.Keywords: cocoa, endophytic fungi, highlands and lowlands

PENGARUH EKSTRAK BUNGA FLAMBOYAN (Delonix regia Hook Raf) TERHADAP PERKECAMBAHAN DAN PERTUMBUHAN CABAI MERAH BESAR (Capsicum annuum L.) Royana Pakpahan; Ni Putu Adriani Astiti; I.G.A. Sugi Wahyuni
SIMBIOSIS Vol 5 No 1 (2017)
Publisher : Jurusan Biologi Universitas Udayana

The reach aim to the effect of flamboyant (Delonix regia Hook Raf.) flower extract on the growth of large red pepper plant (Capsicum annuum L.), for use a land under of flamboyant trees. The research design used was Random Block Design, with five treatments extract concentration, control, 5%, 10%, 15%, and 20%, each treatment was repeated five time. Observations were made during the 15 weeks. The results showed the percentage of germination and plant height are effect significant, while the number of leaves, root length and dry weight of the plant does not provide effect for the growth of large red pepper plant. Keywords : flamboyant, allelopathy, large red pepper

Analysis of Phenolic and Tannin Contents in the Methanol Extract of Sweet and Sour Star Fruit Plants (Averrhoa carambola L) Leaves Commonly Used as Raw Materials of Lawar (A Balinese Traditional Food) Ni Putu Adriani Astiti; Sang Ketut Sudirga; Yan Ramona
Advances in Tropical Biodiversity and Environmental Sciences Vol 3 No 1 (2019): ATBES
Publisher : Institute for Research and Community Services Udayana University

Lawar, a special Balinese food made of leaves of star fruit plant, is commonly served in any Balinese traditional events, such as temple festival or wedding parties. The leaves of sweet and sour star fruit plant are used to make this traditional food. In this research, phenolic and tannin content of leaves of these types of plant were analyzed with a view to investigate their potential as antioxidant source. Phenolic compound has been well known to have antioxidant activity by neutralizing free radicals and stabilizing singlet oxygen. This compound has capacity to reduce free radicals by chelating metal ions or inhibiting enzymatic system, such as cyclo-oxygenase, mono-oxygenase or xanthine oxidase that catalyze formation of free radicals. [4] reported that plants with high content of phenolic compounds tend to have high nutritious and antioxidant activities Tannin is a compound belongs to polyphenolic group. Such compound is abundantly found in any plants. This compound has capacity to bind proteins, alkaloid, and gelatin. In human body, this compound play a very complex role, such as chelating metal ions, precipitating proteins, and functioning as biological antioxidant. The results of this study showed that the phenol content of sour and sweet star fruit plants were 2830.99/100 mg GAE and 1959.77/100 mg GAE, respectively, while the tannin content of these two plants were 18810.43 mg/100 gr TAE and 11035.75 mg/100 gr TAE, respectively, indicating that the leaves of sour star frit plant had better antioxidant activity than the sweet one.

Determination of Flavonoid, Tannin and Vitamin C Content from Methanol Extract Wrapping Stone Banana (Musa brachycarpa), Ketip Banana (Musa Paradisiaca Forma Typiaca) and Kepok Banana (Musa acuminata) Ni Putu Adriani Astiti; Dwi Ariani Yulihastuti
Advances in Tropical Biodiversity and Environmental Sciences Vol 1 No 2 (2017): ATBES
Publisher : Institute for Research and Community Services Udayana University

Research has been done to investigate the levels of flavanoid, tannin, and vitamin C of methanol extract of Stone banana (Musa brachycarpa), Ketip banana (Musa paradisiaca forma typiaca ), and Kepok banana (Musa acuminata). Jukut Ares is a Balinese traditional cuisine frequently served in any events of celebrations. The use of young stems of such banana varieties as the main raw materials in the process of making jukut ares has not been scientifically elucidated. Antioxidants are compounds with ability to inhibit oxidation-related reactions due to free radicals that cause disturbance of saturated fatty acids of cell membrane, blood vessels, DNA, as well as adipose tissues which lead to occurrence of various diseases. Plants components will have antioxidant activities if they contain compounds (phenol and flavanoid) with ability to scavenge free radicals. Tannin is a water based phenolic compound and vitamin C is also a water based vitamin that play important roles in preventing us from various diseases. Extraction was conducted by applying maceration method and to investigate the levels of flavanoid, spectrophotometric UV vis with aluminum chloride (AlCl3) was applied. The level of vitamin C of the materials was conducted by Iodine titration method, while the level of tannin was determind by applying spectrophotometri. The results showed that methanol extract of Ketip banana stem contained the highest level of flavanoid with a quantity of 53.13 mg/100gr QE, and this was followed by stone banana (36.28) and kepok banana (32.07) The highest vitamin C level was indicated in the extract of ketip banana (418.32 mg/100gr), which is followed by kapok banana and stone banana. In the calculation of tannin content, pisang ketip showed the highest level, while pisang batu contained the lowest level of tannin.

Lung Histopathology of Laying Hens Infected by Colibacillosis in the Animal Cages Experiments of the Disease Investigation Center 6, Denpasar, Bali Kadek Ayu Trisna Yanti; Iriani Setyawati; Ni Putu Adriani Astiti
Advances in Tropical Biodiversity and Environmental Sciences Vol 3 No 2 (2019): ATBES
Publisher : Institute for Research and Community Services Udayana University

This study aimed to determine the lungs histopathology of laying hens (Gallus gallus domesticus) at the Animal Cage Experiments in the Disease Investigation Center 6, Directorate General of Live Stock (DIC-6 DGLS), Denpasar, Bali, which died from colibacillosis infection. Sample of lungs were cut transversely then put into 10% of Neutral Buffer Formalin, then processed histologically by paraffin method and stained with Hematoxylin-Eosin. Observation under microscope (magnification 100x and 400x) was done for histopathological examination. Laying hens died from colibacillosis infection showed that their lungs were infected by colibacillosis, and there were found 62.50% of necrosis, 75% of inflammatory cells infiltration and 80% of hemorrhage in the lungs.

Efektifitas daun salam dalam mengurangi cemaran mikroba penyebab busuk telur itik Yulidia Iriani; Yan Ramona; Ni Putu Adriani Astiti
Intisari Sains Medis Vol. 13 No. 2 (2022): (In Press : 1 August 2022)
Publisher : DiscoverSys Inc.

Background: Pathogenic bacteria on eggshells or egg contents will be very dangerous for the health of humans who consume them. Chemicals should not be an option for preservatives because they will cause side effects. In this study, we explored the possibility of using bay leaf extract (Syzygium polyanthum (Wight) Walp) to control the growth of polluting bacteria in salted duck eggs so that the shelf life can be extended. The purpose of the study was to determine the potential of the active compounds contained in bay leaves in reducing the total plate count/microbial contamination in salted duck eggs. Methods: Extraction of bay leaf powder was carried out by the infusion method. The activity test of bay leaf decoction (ARDS) was carried out using the in vitro diffusion well method on Mueller Hinton media. Identification of active compounds was carried out using the GCMS technique. Results: ARDS crude extract at a concentration of 5% (v/v) could significantly reduce ALT and inhibit the in vitro growth of Proteus mirabilis bacteria at a concentration of 10% (w/v), with an inhibition zone diameter of 19.53 mm, with the minimum value inhibitory concentration (MIC) 0.7%. The results of the GCMS analysis of ARDS showed that the ten peaks produced, possibly synergized, and had antimicrobial activity. These compounds are 2-Pentanone4-Hydroxy-4 Methyl (CAS) Diacetone Alcohol; Ethanol, 2-(Ethoxythoxy) (CAS); 1,2,3 Propanetrriol Cas) Glycerol; Citronella (2,6-Octadien-1-Ol, 3,7-Dimethyl, (CAS); Beta Citronellol 6-Octen-1-Ol, 3,7 Dimethyl-(CAS); Trans Geraniol; Dodecanoic Acid, Methyl Ester (CAS) Methyl Laurate; Tetradecanoic Acid, Methyl Ester (CAS) Methyl Myristate; 1-Tetradecanol (Cas) Alfol 14 and Hexadecanoic Acid Methyl Ester CAS) Methyl Palmitate Conclusion: Leaf decoction can inhibit the growth of Proteus mirabilis in vitro, and the addition of ARDS with a concentration of 5% (v/v) can significantly reduce the value of bacterial contamination in salted duck eggs. Latar Belakang: Bakteri pathogen pada kulit telur atau isi telur akan sangat membahayakan kesehatan manusia yang mengkonsumsinya. Bahan kimia seharusnya tidak menjadi pilihan untuk pengawet, karena akan menimbulkan efek samping, Pada penelitian ini dieksplorasi, kemungkinan dipakainya ekstrak daun salam (Syzygium polyanthum (Wight) Walp) untuk mengontrol pertumbuhan bakteri pencemar pada telur itik yang diasinkan, sehingga masa simpannya dapat diperpanjang. Tujuan penelitian untuk mengetahui potensi senyawa aktif yang terdapat pada daun salam dalam menurunkan angka lempeng total/cemaran mikroba pada telur itik yang diasinkan. Metode: Ekstraksi serbuk daun salam dilakukan dengan metode infusa. Uji aktifitas air rebusan daun salam (ARDS) dilakukan dengan metode sumur difusi secara in vitro pada media Mueller Hinton. Identifikasi senyawa aktif dilakukan dengan teknik GCMS. Hasil: Ekstrak kasar ARDS pada konsentrasi 5% (v/v) secara nyata dapat menurunkan ALT dan menghambat pertumbuhan in vitro bakteri Proteus mirabilis pada konsentrasi 10% (b/v), dengan diameter zona hambat sebesar 19,53 mm, nilai minimum inhibitory concentration (MIC) 0,7%. Hasil analisis GCMS terhadap ARDS menunjukan bahwa sepuluh puncak yang dihasilkan, kemungkinan bersinergi dan mempunyai aktifitas sebagai antimikroba. Senyawa-senyawa tersebut adalah 2-Pentanone4-Hydroxi-4 Methyl (CAS) Diacetone Alkohol; Ethanol, 2-(Ethoxythoxy) (CAS); 1,2,3 Propanetrriol Cas) Glycerol; Citronella (2,6-Octadien-1-Ol, 3,7-Dimethyl, (CAS); Beta Citronellol 6-Octen-1-Ol, 3,7 Dimethyl-(CAS); Trans Geraniol; Dodecanoic Acid, Methyl Ester(CAS) Methyl Laurate; Tetradecanoic Acid,Methyl Ester (CAS) Methyl Myristate; 1-Tetradecanol (Cas) Alfol 14 dan Hexadecanoic Acid Methyl Ester CAS) Methyl Palmitate. Simpulan: Air rebusan daun dapat menghambat pertumbuhan Proteus mirablis secara in vitro dan penambahan ARDS dengan konsentrasi 5% (v/v) secara signifikan dapat menurunkan nilai cemaran bakteri (angka lempeng total) pada telur itik yang diasinkan.

Co-Authors A.A.S. A. Sukmaningsih AA Istri Mas Padmiswari Bernadina Metboki Deglory Tunmuni Dwi Ariani Yulihastuti ENIEK KRISWIYANTI I Gusti Ayu Sugi Wahyuni I Ketut Ginantra I Ketut Suada IDA AYU ASTARINI Ida Ayu Meva Lianawati Ida Ayu Nopiari Ida Bagus Gede Darmayasa IRIANI SETYAWATI Jeremy Pramana Kadek Ayu Trisna Yanti Lilis Riana Tambunan Luh Putu Wrasiati MADE PHARMAWATI Made Ria Defiani Meitini Proborini Wahyuni Ngurah Intan Wiratmini Ni Kade Ayu Purnama Adi Ni Komang Tia Pramesti Ni Luh Suriani Ni Made Dewi Wahyuni Ni Made Puspawati Ni Made Sastriyani Wiendra Ni Nyoman Nila Arieswari NI PUTU SRI PUSPITA WIDI YANTHI Ni Wayan Sudatri Pande Putu Purwaningsih Putu Yayun Antari Budaya Retno Kawuri Riana Dyah Suryaningrum Royana Pakpahan SANG KETUT SUDIRGA SARMILA TASNIM Yan Ramona Yuli Setiawati Yulidia Iriani Yulidia Iriani Yully Muharyati

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