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DECOLORIZATION OF TEXTILE DYES WITH CRUDE LIGNINOLYTIC ENZYMES FROM Coriolopsis caperata Agnestisia, Retno; Karelius, Karelius; Oksal, Efriyana; Nion, Yanetri Asi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10931

Abstract

The textile industry is a major contributor to global water pollution, releasing an estimated 280,000 tons of synthetic dyes annually into aquatic ecosystems. These dyes, particularly azo compounds, are often toxic, mutagenic, and resistant to conventional wastewater treatment methods. Microbial enzymatic degradation, especially by white-rot fungi, offers a promising eco-friendly alternative. This study aimed to optimize the production of ligninolytic enzymes—Laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP)—from Coriolopsis caperata, and assess their efficiency in degrading two azo dyes: Reactive Red 21 (RR21) and Reactive Orange 107 (RO107). The fungus, isolated from the Peat Swamp Forest in Sebangau, Central Kalimantan, was cultured in a modified glucose-peptone medium enriched with veratryl alcohol. The optimization parameters included variations in time, dye concentration, and the addition of hydrogen peroxide (H₂O₂). Enzyme activity was quantified spectrophotometrically, and dye decolorization was assessed over time at different dye concentrations. Among the enzymes, Lac showed the highest activity (4938.05 U/L), followed by LiP (995.26 U/L) and MnP (246.47 U/L). These values notably exceed several previously reported benchmarks for fungal enzyme activity. RO107 demonstrated greater susceptibility to enzymatic degradation, with 83.71% decolorization achieved at 24 hours, while RR21 reached 65.71% at 48 hours. The addition of 1 mM H₂O₂ significantly enhanced decolorization, increasing RR21 and RO107 removal to 95.71% and 99.30%, respectively. These results underscore the oxidative synergy between H₂O₂ and ligninolytic enzymes, particularly LiP and MnP. Overall, the study demonstrates the potential scalability of C. caperata-based enzymatic treatment systems for textile effluent bioremediation, supporting compliance with environmental discharge regulations and contributing to sustainable wastewater management.
Keanekaragaman Jamur Makroskopis di Hutan Gambut Arboretum Nyaru Menteng Kalimantan Tengah Desimaria Panjaitan; Frans Grovy Naibaho; Retno Agnestisia; Ivan Permana Putra
Konservasi Hayati Vol 19 No 2 (2023): OKTOBER
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/hayati.v19i2.29404

Abstract

Information on macroscopic fungi from Central Kalimantan is still limited compared to other regions in Indonesia. Arboretum Nyaru Menteng, a conservation area located in Central Kalimantan, poses many macrofungi due to its favorable environmental conditions that support the growth of various types of macrofungi. However, the diversity of macroscopic fungi from Arboretum Nyaru Menteng has never been reported. Thus, the current works aimed to provide initial information on the diversity of macroscopic fungi in this conservation area. The exploration was done using the opportunistic sampling method. Fruiting bodies were documented, collected, and identified based on macroscopic characteristics. Four environmental microclimate variables were measured in four random sampling locations: temperature, light intensity, air humidity, and soil acidity. The results confirmed that there were 27 species of macrofungi belonging to 18 genera and 14 families. Polyporaceae was the most dominant family found in this research. Most fungi grew on a dead log while the others were recognized as mycorrhizal fungi and parasites on plants. The environmental measurements showed that Arboretum Nyaru Menteng has an optimal habitat for recorded fungi growth. The record of macrofungi diversity in this research contributes to Indonesia mycoflora, particularly from Central Kalimantan.
Degradation of Humic Acids in Peat Water Using Fe3O4 Synthesized from Zircon Sand Tailing as Photo-Fenton Catalyst Toepak, Erwin Prasetya; Pasaribu, Marvin Horale; Agnestisia, Retno; Elsandika, Gabriela
Jurnal Kimia Riset Vol. 10 No. 1 (2025): June
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkr.v10i1.66061

Abstract

Zircon sand mining in Indonesia generates magnetic waste rich in magnetite (Fe₃O₄), which has semiconductor properties, making it an effective catalyst for degrading organic pollutants. This study explores the synthesis and application of Fe₃O₄ derived from zircon mining waste as a photo-Fenton catalyst for humic acid (HA) degradation in peat water. The Fe₃O₄ catalyst was synthesized using a co-precipitation method, confirmed by FTIR and XRD analyses. FTIR identified Fe–O bonds at 537 cm-1 and 419 cm-1, while XRD showed a spinel crystal structure with peaks at 2θ angles of 30.14°, 35.51°, 43.19°, and 56.96°. The catalytic activity was tested under UV light with varying H₂O₂ volumes and Fe₃O₄ masses. The optimized condition, using 0.03 g Fe₃O₄ and 1 mL H₂O₂, achieved a maximum HA degradation efficiency of 87.06% in 100 minutes. Kinetic analysis revealed second-order kinetics, with a rate constant (k) of 8.29 × 10-4 M-1.min-1 and R² = 0.9911, showing a strong correlation. The high efficiency is attributed to Fe₃O₄'s semiconductor properties, which facilitate hydroxyl radical formation and enhance electron transfer. These findings demonstrate the potential of Fe₃O₄ from zircon mining waste as a sustainable catalyst for environmental applications, particularly in treating organic pollutants in peat water.
Pendampingan Branding, Packaging, dan Digital Marketing Tingkatkan Penjualan Madu Kelulut Desa Tuwung Yuliana, Yuliana; Agnestisia, Retno; Saputra, Rizki Rachmad; Pasaribu, Marvin Horale
Journal of Appropriate Technology for Community Services Vol. 6 No. 1 (2025)
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jattec.vol6.iss1.art2

Abstract

Tuwung Village, located in the Kahayan Tengah District, Pulang Pisau Regency, Central Kalimantan, has significant potential in managing social forestry land to support food security. The local community, organized in the Social Forestry Business Group (KUPS), has successfully cultivated 120 hives of stingless bees (Trigona sp.), with monthly production capacity of up to 350 mL per hive. Although honey production is abundant, its sales remain very limited due to a marketing strategy that relies solely on word-of-mouth promotion. Therefore, this community service activity was introduced to provide guidance to KUPS Madu Kelulut on branding, product packaging, and digital marketing. With an attractive brand, high-quality packaging, and the right marketing strategy, it is hoped that the stingless bee honey business can become more competitive in a market already saturated with similar products. This community service program involved several stages, including preparation, implementation, and evaluation. The activity was carried out on July 9, 2024, with 40 participants. The evaluation results indicated an 81.25% increase in participants' understanding of branding, product packaging, and digital marketing. Furthermore, the participants successfully created the brand "Madu Kelulut Nyai Hapu," with an appealing packaging design and effective promotional content shared through social media and e-commerce platforms.
DECOLORIZATION OF TEXTILE DYES WITH CRUDE LIGNINOLYTIC ENZYMES FROM Coriolopsis caperata Agnestisia, Retno; Karelius, Karelius; Oksal, Efriyana; Nion, Yanetri Asi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10931

Abstract

The textile industry is a major contributor to global water pollution, releasing an estimated 280,000 tons of synthetic dyes annually into aquatic ecosystems. These dyes, particularly azo compounds, are often toxic, mutagenic, and resistant to conventional wastewater treatment methods. Microbial enzymatic degradation, especially by white-rot fungi, offers a promising eco-friendly alternative. This study aimed to optimize the production of ligninolytic enzymes—Laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP)—from Coriolopsis caperata, and assess their efficiency in degrading two azo dyes: Reactive Red 21 (RR21) and Reactive Orange 107 (RO107). The fungus, isolated from the Peat Swamp Forest in Sebangau, Central Kalimantan, was cultured in a modified glucose-peptone medium enriched with veratryl alcohol. The optimization parameters included variations in time, dye concentration, and the addition of hydrogen peroxide (H₂O₂). Enzyme activity was quantified spectrophotometrically, and dye decolorization was assessed over time at different dye concentrations. Among the enzymes, Lac showed the highest activity (4938.05 U/L), followed by LiP (995.26 U/L) and MnP (246.47 U/L). These values notably exceed several previously reported benchmarks for fungal enzyme activity. RO107 demonstrated greater susceptibility to enzymatic degradation, with 83.71% decolorization achieved at 24 hours, while RR21 reached 65.71% at 48 hours. The addition of 1 mM H₂O₂ significantly enhanced decolorization, increasing RR21 and RO107 removal to 95.71% and 99.30%, respectively. These results underscore the oxidative synergy between H₂O₂ and ligninolytic enzymes, particularly LiP and MnP. Overall, the study demonstrates the potential scalability of C. caperata-based enzymatic treatment systems for textile effluent bioremediation, supporting compliance with environmental discharge regulations and contributing to sustainable wastewater management.
Co-Authors Abdul Hadjranul Fatah Abdul Hadjranul Fatah Abed Nego Purba Abudarin Abudarin Abudarin Abudarin Adventus Panda Akhmad Damsyik Akhmad Damsyik Alfanaar, Rokiy Ansori Rahman Ariska, Risya Ayuchecaria, Noverda Chuchita Damsyik, Akhmad Deni Shidqi Khaerudini Desimaria Panjaitan Dessy Analinta Dewi, Sintia Ayu Diah Ayu Pramoda Wardani Dicky Perwira Ompusunggu Edy Prasetyo Eka Jhonatan Krissilvio Eka Jhonatan Krissilvio Elsandika, Gabriela Erwin Prasetya Toepak Erwin Prasetya Toepak Erwin Prasetya Toepak Erwin Prasetya Toepak F. Adany Frans Grovy Naibaho Hakim, Muh. Supwatul I Made Sadiana, I Made I Nyoman Sudyana I Nyoman Sudyana I Nyoman Sudyana I Nyoman Sudyana I Nyoman, Sudyana, Ilham Maulana Indri Susanti Iqbal, Rendy Muhamad Ivan Permana Putra Jumiati Jumiati Junita Dongoran Karelius Karelius Karelius Karelius Karelius Karelius Karelius, Karelius Marvin Horale Pasaribu Midun Efendi Patar Sihombing Mokhamat Ariefin Muhamad Iqbal, Rendy Muhammad Irmawan Narsito Narsito Noer Komari Nyahu Rumbang Oksal, Efriyana Okta Prianus Prianus, Okta Purba, Abed Nego Rasidah Rasidah, Rasidah Remi Ayu Pratika Rendy Muhamad Iqbal Rendy Muhamad Iqbal Rendy Muhamad Iqbal Riandy Putra Risfiah Ruli Cahyani Rizki Rachmad Saputra Rizkita, Aden Dhana Sinaga, Tiara Cristy Agatha Siswo Siswo Soaloon Sinaga Sri Yulandari Simangunsong Sugeng Mashabhi sunardi sunardi Sunardi Sunardi Suyanta Suyanta Syahrul Razikin Tampubolon, Mulani Jeni Lestari Wahyu Nugroho Wahyu Supriyati Wayan Adhi Nareyasa Whendy Trissan Widya Krestina, Luqman Hakim, Dyah Ayu Pramoda Wardani, Wulandari, Ika Octavia Yanetri Asi Nion, Yanetri Asi Yuliana Yuliana Yuliana Zimon Pereiz