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All Journal Jurnal Ilmu Pertanian Indonesia Biospecies Jurnal Natur Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Jurnal Agroindustri JURNAL BIOLOGI INDONESIA Jurnal Biodjati JURNAL SELULOSA Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Konservasi Hayati Jurnal Ilmiah Biologi UMA (JIBIOMA) Jurnal Jaring SainTek BIOVALENTIA: Biological Research Journal Jurnal Kedokteran Hewan Organisms: Journal of Biosciences AMMA : Jurnal Pengabdian Masyarakat Laporan Upaya Nyata Inovasi Ilmu Komputer (LUNIK) Bioma : Jurnal Biologi dan Pembelajaran Biologi Analit: Analytical And Environmental Chemistry
Rochmah Agustrina
Universiy of Lampung, Jln. Soemantri Brojonegoro 1, Bandar Lampung 35145
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Social Sciences Veterinary Other

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Efektivitas Ekoenzim Kulit Pisang Kepok Manado Muda sebagai Insektisida Nabati terhadap Hama Penghisap Buah Kakao (Helopeltis sp.) Agustin, Meli; Pratami, Gina Dania; Priyambodo; Agustrina, Rochmah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2025): February 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.9288

Abstract

Kakao merupakan tanaman perkebunan yang mempunyai peran cukup penting dalam perekonomian nasional. Salah satu kendala pada budidaya kakao adalah kehadiran organisme pengganggu tanaman, yaitu kepik penghisap buah kakao (Helopeltis sp.). Pemakaian insektisida kimia secara berlebihan dapat menimbulkan dampak negatif sehingga diperlukan insektisida alternatif yang ramah lingkungan. Ekoenzim kulit pisang kepok manado muda memiliki kandungan bahan aktif yang berpotensi dijadikan insektisida nabati. Penelitian ini bertujuan untuk mengetahui konsentrasi ekoenzim kulit pisang kepok manado muda yang efektif dalam mematikan hama penghisap buah kakao. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) faktorial 2 faktor perlakuan dan 3 ulangan. Faktor pertama adalah konsentrasi ekoenzim (0%, 5%, 10%, 15%, dan 20%). Faktor kedua adalah waktu pengamatan (6, 12, 24, 48, dan 72 jam). Data hasil pengamatan dianalisis menggunakan ANOVA dengan uji lanjut Tukey dan nilai LC50 dianalisis menggunakan probit. Hasil penelitian menunjukkan bahwa ekoenzim kulit pisang kepok manado muda berpotensi sebagai insektisida nabati terhadap hama penghisap buah kakao dengan konsentrasi paling efektif adalah 20%. Sementara hasil analisis probit didapatkan nilai LC50 sebesar 17,95% pada waktu pengamatan 72 jam.
Colchicine Effect of Mitosis Root Tip by Banana Kepok Yellow (Musa paradisiaca L.) Polyploid Explants Yana, Dellya Vivi; Ernawiati, Eti; Agustrina, Rochmah; Handayani, Tundjung Tripeni
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 1 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i1.210

Abstract

Colchicine can inhibit spindle formation so that doubled chromosomes fail to separate during anaphase cytologically to induce mutations that produce polyploid plants. Yellow kepok banana is a triploid banana. The supply of yellow kepok bananas is hampered by conventional cultivation methods and the limited availability of quality seeds. This study aims to determine the effect of adding colchicine compound to tissue culture media on the mitotic index, chromosome number, and chromosomal abnormalities in yellow kepok bananas and the formation of polyploid plantlets. This study was included in the experimental study by conducting mitotic preparation and observing morphology after the test sample was induced by colchicine. The results of the research that has been done show that the addition of the compound colchicine to the tissue culture medium of yellow kepok banana causes a decrease in the mitotic index, an increase in the number of chromosomes, the presence of chromosomal abnormalities, the formation of polyploid plantlets, and an increase in the number of roots, but there is a reduction in root length.
Terathogenic Testing of Black Cumin (Nigella sativa L.) Extract on The Number of Fetuses, Length of Front and Back Extremities, and The Number of Malformation Fetus in Mice (Mus musculus L.) Fardhira, Zikra; Nurcahyani, Nuning; Agustrina, Rochmah; Tugiyono, Tugiyono
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 1 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i1.218

Abstract

Pemakaian obat tradisional telah berkembang dengan baik dan saat ini sudah banyak digunakan sebagai obat alternatif untuk mengatasi masalah kesehatan. Obat tradisional akan memberikan khasiat yang baik apabila dikonsumsi dengan cara dan dosis yang tepat untuk tujuan pengobatan. Berdasarkan penelitian sebelumnya pemberian jintan hitam (Nigella sativa L) terhadap mencit betina menyebabkan terjadinya aktivitas antifertilisasi, antinociceptif, kontrasepsi postcoitus, dan antioksitosin yang diduga dapat melancarkan menstruasi. Tingkat keamanan konsumsi jintan hitam pada masa kehamilan terhadap perkembangan fetus, serta sistem reproduksi mencit betina yang menimbulkan malfomasi congenital pada anaknya belum banyak diteliti. Tujuan dari penelitian ini untuk mengetahui pengaruh ekstrak jintan hitam (Nigella sativa L) terhadap jumlah fetus mencit,ukuran panjang ekstremitas depan dan belakang pada fetus mencit, dan jumlah fetus malformasi pada  mencit. Penelitian ini menggunakan 20 ekor  mencit betina yang sudah bunting dan dibagi menjadi 4 kelompok yaitu kelompok K (kontrol) diberi 0,3 mL aquabides, kelompok perlakuan P1 dengan dosis 2,1 mg/g BB dalam 0,3 mL aquabides, kelompok perlakuan P2 dengan dosis 8,4 mg/g BB dalam 0,3 mL aquabides, dan kelompok perlakuan P3 dengan dosis 33,6 mg/g BB dalam 0,3 mL aquabides. Perlakuan diberikan pada hari ke 6  sampai ke 17 kebuntingan. Hasil menunjukkan bahwa pemberian ekstrak jintan hitam tidak memberikan pengaruh terhadap jumlah fetus yang dikandung dan tidak menurunkan  pertumbuhan ekstrimitas depan dan belakang fetus mencit karena tidak menunjukkan hasil yang signifikan antara kelompok kontrol (K) dan kelompok perlakuan (P1, P2, dan P3). Pemberian ekstrak jintan hitam (Nigella sativa L.) tidak meningkatkan jumlah fetus malformasi akan tetapi menyebabkan adanya fetus yang mati pada saat kelahiran dan mengalami malformasi pada beberapa fetus lainnya.     The use of traditional medicine has developed well and is now widely used as an alternative medicine to treat health problems. Traditional medicine will provide good efficacy if consumed in the right way and dosed for medicinal purposes. Based on previous research, giving black cumin (Nigella sativa L) to female mice caused infertility, antinociceptive, postcoitus contraception, and anti-oxytocin activities which were thought to be able to launch menstruation. The safety level of consumption of black cumin during pregnancy on fetal development, as well as the reproductive system of female mice that cause congenital malformations in their offspring, has not been widely studied. The purpose of this study was to determine the effect of black cumin extract (Nigella sativa L) on the number of mice fetuses, the length of the front and back extremities in mice fetuses, and the number of malformed fetuses in mice. This study used 20 pregnant female mice and was divided into 4 groups, namely group K (control) was given 0.3 mL aquabidest, treatment group P1 with a dose of 2.1 mg/g BW in 0.3 mL aquabidest, treatment group P2 with a dose of 8.4 mg/g BW in 0.3 mL aquabidest, and the P3 treatment group with a dose of 33.6 mg/g BW in 0.3 mL aquabidest. The treatment was given on the 6th to 17th day of pregnancy. The results showed that the administration of black cumin extract had no effect on the number of fetuses conceived and did not reduce the growth of the front and rear extremities of the mice fetuses because it did not show significant results between the control group (K) and the treatment group (P1, P2, and P3). The administration of black cumin extract (Nigella sativa L.) did not increase the number of malformed fetuses but caused fetal death at birth and malformations in several other fetuses.      
Penentuan Karakteristik dan Efektivitas Eco Enzyme Berbahan Dasar Limbah Organik yang Berbeda sebagai Pengawet Buah Tomat (Solanum esculentum MILL.): Determination of The Characteristics And Effectiveness of Eco enzyme Based on Different Organic Waste For The Preservation of Tomato Fruit (Solanum esculentum Mill) Setiawati, Primasari Linda; Sumardi, Sumardi; Irawan, Bambang; Nurcahyani, Endang; Agustrina, Rochmah
Biospecies Vol. 16 No. 1 (2023): Januari 2023
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v16i1.24110

Abstract

Eco enzyme is a solution of complex organic substances produced by the fermentation of organic waste, sugar and water. Eco enzyme liquid is dark in color and has a strong sour/fresh aroma (Hemalitha and Visantini, 2013). Eco enzyme contains acid and alcohol compounds which act as antimicrobials. The aim of this research was to determine the characteristics of eco enzyme based on five organic wastes, namely eco enzyme made from banana peels, eco enzyme made from lemon peel, eco enzyme made from pineapple peel, eco enzyme made from pieces of spinach stems and leaves, and eco enzyme made from cassava leaves and to know the effectiveness of eco enzyme in the preservation process of tomatoes. The study consisted of 2 research phases, namely quantitatively determining the characteristics of eco enzyme based on five organic materials and determining the effectiveness of the eco enzymeter in the preservation process of tomatoes. of 3.42. The highest total acid was found in the P5 treatment of 5.25%, the highest LAB and yeast populations were found in the P4 treatment, namely 2.34 Log CFU/ml and 2.02 Log CFU/ml. The effectiveness of the five eco enzyme products on the best preservation of tomatoes was found in the P4 treatment, which was 70%. Keywods: Eco Enzyme, Organic Waste, Tomatoes.   Abstrak Eco enzyme merupakan larutan zat organik kompleks yang dihasilkan oleh fermentasi limbah organik, gula dan air. Cairan eco enzyme berwarna gelap dan memiliki aroma asam/segar yang kuat (Hemalitha dan Visantini, 2013). Eco enzyme mengandung senyawa asam dan alkohol yang sangat berperan sebagai antimikroba. Penelitian ini bertujuan untuk mengetahui karakteristik eco enzim berbasis lima limbah organik, yaitu eco enzyme berbahan dasar kulit pisang, eco enzyme berbahan dasar kulit jeruk lemon, eco enzyme berbahan dasar kulit nanas, eco enzyme berbahan dasar potongan batang dan daun bayam, dan eco enzyme berbahan dasar daun singkong serta mengetahui efektifitas eco enzyme terhadap proses pengawetan buah tomat. Penelitian terdiri dari 2 tahap penelitian yaitu, penentuan karakteristik eco enzyme yang berbasis lima bahan organik secara kuantitatif dan penentuan efektifias eco enzyme terhadap proses pengawetan buah tomat. Hasil penelitian menunjukkan karakteristik eco enzyme berbasis lima limbah organik yaitu keasaman (pH) tertinggi terdapat pada perlakuan P2 sebesar 3,42. Total asam tertinggi terdapat pada perlakuan P5 sebesar 5,25%, populasi BAL dan populasi yeast tertinggi terdapat pada perlakuan P4 yaitu 2, 34 Log CFU/ml dan 2,02 Log CFU/ml. Efektifitas lima produk eco enzyme terhadap tingkat keawetan buah tomat terbaik terdapat pada perlakuan P4 yaitu 70 %. Kata Kunci: Eco Enzyme, Limbah Oranik, Buah Tomat.
Pengaruh Ekstrak Batang Bandotan (Ageratum conyzoides L.) Terhadap Pertumbuhan Fusarium oxysporum yang Menginfeksi Tanaman Cabai Merah Besar (Capsicum annuum L.) Fadhilla, Nurul; Agustrina, Rochmah; Chrisnawati, Lili; Mahfut, Mahfut
Konservasi Hayati Vol 19 No 2 (2023): OKTOBER
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/hayati.v19i2.26597

Abstract

Fusarium oxysporum is a fungal pathogen that causes plant wilt which infects many horticultural crops, including red chili (Capsicum annum L.). The continual control of pathogenic fungus with synthetic pesticides can have a negative impact on the environment, so it is necessary to look for safe and environmentally friendly alternative pathogen control methods, one of which is by using natural pesticides. Bandotan (Ageratum conyzoides L.) is known as a weed, containing secondary metabolites with antifungal activity. This study aims to determine the effect of bandotan stem extract on the growth of Fusarium oxysporum in infected chili plants during the germination phase. The study was conducted using a Completely Randomized Design (CRD) with one treatment factor, namely the method of giving bandotan extract consisting of 5 replications. ANOVA results at α = 5% showed that 65% dry bandotan stem extract did not reduce Fusarium oxysporum growth on the red chili plants.
Cytological analysis of Kepok Abu Polyploid's Banana Plantlets (Musa paradisiaca L.) Due to Colchicine's Application in Tissue Culture Media istiqomah, Istiqomah; Wahyuningsih, Sri; Ernawiati, Eti; Agustrina, Rochmah
Biosfer: Jurnal Tadris Biologi Vol 12 No 2 (2021): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v12i2.8501

Abstract

This research aimed to determine the mitotic abnormalities, changes in the number of chromosomes, mitotic index, and the number and length of kepok abu banana plantlet roots due to administration of 0.1% colchicine in tissue culture media. This research was performed at the Botanical Laboratory of the Biology Department, Faculty of Mathematics and Natural Sciences, UNILA. This research compared the cytology by employing the Squash method between root tip cells of kepok abu banana plantlets after administering 0.1% colchicine and the control group. The researchers analyzed the data descriptively and presented them in tables and bar charts. The results showed that the administration of 0.1% colchicine resulted in mitotic abnormalities. It also increased the number of chromosomes in the controls 2n=3x and 2n=3x+4. The mitotic index decreased, and the average number of roots was lower. However, the size was longer compared to the control group.
Induksi Poliploid Planlet Pisang Kepok Batu Dengan Media Kultur Jaringan Sabana, Amaliya; Ernawiati, Eti; Priyambodo, Priyambodo; Agustrina, Rochmah
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 2 No. 1 (2022): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/organisms.v2i1.10243

Abstract

Colchicine is often used in plant breeding to induce mutations and produce polyploid plants with superior traits. Cytologically polylpoid plants have variations in shape, number of chromosomes and larger cell sizes. The use of colchicine in kepok batu bananas is expected to produce polyploid banana kepok batu plants. The purpose of this study was to determine the effect of colchicine compounds on mitotic abnormalities, changes in chromosome number, decreased mitotic index, and the number and length of roots in banana kepok batu plantlets formed on tissue culture media. The research was carried out at the Botanical Laboratory, Department of Biology, FMIPA Unila. This study compared cytology with the squash method between root tip cells of banana kepok batu plantlets after the addition of 0.1% colchicine with controls. Data were analyzed descriptively and displayed in the form of tables and bar charts. The results showed that administration of 0.1% colchicine resulted in mitotic abnormalities (mitotic abnormalities) and increased the number of chromosomes in the control 2n=3x or 33 and 0.1% colchicine treatment, namely 2n=3x+6 or 39. The mitotic index decreased, but averaged – the average number of roots was more and their size was longer than the control. From these results it can be concluded that the administration of 0.1% colchicine was able to induce polyploid cells in banana kepok batu plantlets.AbstrakKolkisin sering digunakan dalam pemuliaan tanaman untuk menginduksi mutasi dan menghasilkan tanaman poliploid dengan sifat unggul. Secara sitologi tanaman polilpoid memiliki variasi bentuk, jumlah kromosom serta ukuran selnya lebih besar. Penggunaan kolkisin pada pisang kepok batu diharapkan dapat menghasilkan tanaman pisang kepok batu poliploid. Tujuan penelitian ini untuk mengetahui pengaruh senyawa kolkisin terhadap abnormalitas mitosis, perubahan jumlah kromosom, penurunan indeks mitosis, serta jumlah dan panjang akar pada planlet pisang kepok batu yang terbentuk pada media media kultur jaringan. Pelaksanaan penelitian di Laboratorium Botani Jurusan Biologi FMIPA Unila. Penelitian ini membandingkan sitologi dengan metode squash antara sel ujung akar planlet pisang kepok batu setelah penambahan kolkisin 0,1% dengan kontrol. Data dianalisis secara deskriptif dan ditampilkan dalam bentuk tabel serta diagram batang. Hasil penelitian menunjukkan bahwa pemberian kolkisin 0,1% memunculkan kelainan mitosis (abnormalitas mitosis) serta meningkatkan jumlah kromosom pada kontrol 2n=3x atau 33 dan perlakuan kolkisin 0,1% yaitu 2n=3x+6 atau 39. Indeks mitosisnya menurun, namun rata – rata jumlah akar lebih banyak dan ukurannya lebih panjang dibandingkan dengan kontrol. Dari hasil tersebut dapat disimpulkan bahwa pemberian kolkisin 0,1% mampu menginduksi sel – sel poliploid pada planlet pisang kepok batu.
Efektivitas Ekoenzim Kulit Pisang Kepok Manado Terhadap Mortalitas Kutu Putih Tanaman Pepaya Pratiwi, Rahayu Fathanah; Pratami, Gina Dania; Mumtazah, Dzul Fithria; Agustrina, Rochmah
Bioma : Jurnal Biologi dan Pembelajaran Biologi Vol. 9 No. 2 (2024): BIOMA: JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v9i2.2467

Abstract

Kutu putih (Paracoccus marginatus Williams & Granara de Willink, 1992) merupakan hama penting dalam budidaya tanaman pepaya di Indonesia. Kandungan metabolit sekunder dalam kulit pisang kepok Manado (Musa x paradisiaca L.) berpotensi sebagai zat toksik bagi serangga. Penelitian ini bertujuan untuk mengetahui pengaruh dan konsentrasi ekoenzim dari kulit pisang kepok manado yang efektif dalam menekan mortalitas hama kutu putih. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan 5 perlakuan konsentrasi yaitu 0%, 5%, 10%, 15%, dan 20%. Setiap perlakuan dilakukan 3 kali ulangan menggunakan 10 ekor kutu putih setiap ulangan. Mortalitas kutu putih diamati pada jam ke 6, 12, 24, 48, dan 72 jam setelah perlakuan. Data dianalisis menggunakan analisis probit untuk menentukan nilai LC50 dan Analysis of Variance (ANOVA) dengan taraf 5% serta uji lanjut dengan uji Tukey’s. Hasil analisis menunjukkan ekoenzim dari kulit pisang kepok manado konsentrasi 20% berpengaruh nyata dan menghasikan mortalitas tertinggi hama kutu putih pepaya. Selain itu, berdasarkan nilai LC50, ekoenzim dari kulit pisang kepok manado efektif dalam mematikan hama kutu putih pepaya setelah 48 jam perlakuan dengan nilai LC50 sebesar 14,71%.
ISOLASI, IDENTIFIKASI DAN PEMBUATAN INOKULUM FUNGI SEBAGAI PENDEGRADASI LIMBAH PADAT TANAMAN TEBU (Saccharum officinarum L.) Supriyanti, Arni; Sumardi; Yusnaini, Sri; Agustrina, Rochmah; Handayani, Kusuma; Musa; Pramana, Yanuar Sigit
Jurnal Agroindustri Vol. 15 No. 1 (2025): May 2025
Publisher : BPFP Faperta UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jagroindustri.15.1.97-111

Abstract

Penelitian ini bertujuan untuk memperoleh isolat fungi yang mampu dalam mendegradasi bagas tebu. Mengetahui pengaruh substrat jagung dan onggok terhadap pertumbuhan isolat fungi. Mengetahui bobot dan ratio C/N bagas tebu yang diberi inokulum fungi yang diambil dari media jagung dan onggok. Penelitian dilakukan di Laboratorium Mikrobiologi Universitas Lampung. Bulan November 2023 s.d April 2024.  Penelitian dilakukan secara faktorial dalam Rancangan Acak Lengkap (RAL).  Faktor pertama jenis substrat; jagung dan onggok.  Faktor kedua adalah isolat yang digunakan yaitu isolate Trichoderma sp komersial isolat fungi diduga Trichoderma sp., isolat diduga fungi Coprinus sp., isolat diduga fungi Neurospora sp., dengan pengulangan 3 kali.  Parameter yang diamati adalah jumlah inokulum, bobot degradasi bagas tebu dan rasio C/N.  Data heterogen dan tidak normal, dilakukan uji Kruskal-Wallis, dilanjutkan dengan uji lanjut Mann-Whitney pada taraf 5%. Uji Kruskal-Wallis tidak menunjukkan adanya beda nyata akibat perlakuan.  Hasil yang diperoleh adalah isolat yang mampu mendegradasi bagas tebu, yaitu diperolehnya sembilan isolat, tujuh diantaranya termasuk genus Trichoderma, satu genus Coprinus dan satu genus Neurospora. ubstrat jagung dan onggok dapat digunakan sebagai media alternatif dalam pembuatan inokulum fungi, dimana keduanya tidak menunjukkan perbedaan yang signifikan terhadap respon jumlah inokulum.  Penurunan bobot serta rasio C/N selama 28 hari dengan pemberian inokulum fungi tidak menunjukkan hasil yang baik.
THE EFFECT OF MAGNETIC FIELD ON ANTIBIOTIC INHIBITION FOR Escherichia coli AND Bacillus sp. Sumardi, Sumardi; Agustrina, Rochmah; Ekowati, Christina Nugroho; Putra, Rizani Oktanisyah; Hartono, Madi
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i1.8051

Abstract

This study was aimed to test the growth of bacteria Escherichia coli (E. coli) and Bacillus sp. which were exposed to magnetic fields. In the first stage of the study, the effect of magnetic fields on the growth of E. coli and Bacillus sp were observed. The futher study was aimed to evaluate the effect of magnetic field on antibiotic sensitivity againts the growth of E. coli and Bacillus sp. The magnetic fields treatments were 0.0 mT (control), 0.1 mT, 0.2 mT, 0.3 mT which were exposed for 10 hours for each treatment. Five antibiotics (trimethoprim, ampicillin, nalidixic acid, streptomycin and chloramphenicol) were used for each bacteria. The result showed that the magnetic field did not influence the colony growth of E.coli, but in Bacillus sp. was seen the increasing of colony area in magnetic field of 0.1 mT and 0.2 mT compared with control treatment. Antibiotic of trimethoprim, nalidixic acid, and ampicillin increase the growth inhibition of E. coli when the bacteria have been exposed to 0.1 mT; 0.2 mT; 0.3 mT magnetic field for 10 minutes. The inhibition by streptomycin and chloramphenicol antibiotic on E. coli did not affected by magnetic field exposure. The inhibition of Bacillus sp by trimethoprim and ampicillin increased when the bacteria have been exposed to 0.2 mT and 0.3 mT magnetic field for 10 minutes. The inhibition of nalidixic acid, streptomycin and chloramphenicol to Bacillus sp. did not affected by the magnetic field exposure.
Co-Authors A.A. Ketut Agung Cahyawan W Agustin, Meli Ajeng Pratiwi Bambang Irawan Christina Nugroho Ekowati, Christina Nugroho Dania Pratami, Gina DEWI SARTIKA Endang Nurcahyani Erika Erika Ernawiati, Eti Fadhilla, Nurul Fardhira, Zikra Farisi, Salman Firda Safira, Masnoni Fithria Mumtazah, Dzul Gina Dania Pratami, Gina Dania istiqomah istiqomah Kusuma Handayani, Kusuma Lili Chrisnawati, Lili Madi Hartono Maemonah, Maemonah Mahfut Mohammad Kanedi, Mohammad Mulyono Mulyono Mumtazah, Dzul Fithria Musa Mutmainah, Nurul Aniqotun Novitasari, Vina Nuning Nurcahyani Nur Qolbi, Hana Pramana, Yanuar Sigit Pratiwi, Rahayu Fathanah Priyambodo Priyambodo Priyambodo Priyambodo Priyambodo Putra, Rizani Oktanisyah Putri, Desma Ramadhina Putri, Jean Delliana Rosa, Emantis RR. Ella Evrita Hestiandari Sabana, Amaliya Sazilly, Muhamad Rizkci Sembiring, Rinawati Setiawati, Primasari Linda Sitinjak, Mentoria Alviani Sri Wahyuningsih Sri Yusnaini Sumardi Sumardi . Supriyanti, Arni Sutyarso Tamara, Derlian Ella Tengku Riza Zarzani N Tugiyono Tugiyono Tundjung Tripeni Handayani, Tundjung Tripeni Yana, Dellya Vivi Yulianty Yulianty YUNITA