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All Journal Jurnal Ilmu Pertanian Indonesia Biospecies Jurnal Natur Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Jurnal Agroindustri JURNAL BIOLOGI INDONESIA Jurnal Biodjati JURNAL SELULOSA Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Konservasi Hayati Jurnal Ilmiah Biologi UMA (JIBIOMA) Jurnal Jaring SainTek Jurnal Penelitian Inovatif BIOVALENTIA: Biological Research Journal Jurnal Kedokteran Hewan Organisms: Journal of Biosciences AMMA : Jurnal Pengabdian Masyarakat Laporan Upaya Nyata Inovasi Ilmu Komputer (LUNIK) Bioma : Jurnal Biologi dan Pembelajaran Biologi Analit: Analytical And Environmental Chemistry
Rochmah Agustrina
Universiy of Lampung, Jln. Soemantri Brojonegoro 1, Bandar Lampung 35145
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Bandotan (Ageratum conyzoides L.) as Bio-Fungicide for Controlling Fusarium oxysporum in Chili (Capsicum annuum L.) Agustrina, Rochmah; Fadhilla, Nurul; Tamara, Derlian Ella; Chrisnawati, Lili; Yulianty, Yulianty; Mahfut, Mahfut
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.27470

Abstract

Fusarium oxysporum L. is a fungus responsible for causing plant wilt disease in various horticultural crops. Meanwhile, Bandotan (Ageratum conyzoides L.) is commonly known as a weed that contains secondary metabolites with antifungal activity. Therefore, this study aimed to determine the effect of Bandotan stem and leaf extract on the growth of Fusarium oxysporum in chili (Capsicum annuum L.) infected during the germination phase. Evaluation of stem and leaf extract was carried out separately using a one-factor Complete Randomized Design (CRD). A total of four experimental units were used consisting of C+ (sprouts not infected with Fusarium sp. and not given stem or leaf of Bandotan extract), C- (sprouts infected with Fusarium sp. but not given stem or leaf of Bandotan extract), T1 (sprouts soaked with stem or leaf of Bandotan extract and infected with Fusarium sp.), and T2 (sprouts infected with Fusarium sp., and given stem or leaf of Bandotan extract). Each experimental unit was repeated five times, and parameters observed included disease severity, as well as dry and wet weight. The results showed that based on ANOVA test at the 5% significance level, the administration of 60% dry Bandotan stem extract to chili sprouts before Fusarium sp. infection significantly reduced disease severity. However, when the treatment was carried out after sprouts were infected, disease severity was not reduced. The administration of Bandotan leaf extract to sprouts both before and after being infected with Fusarium sp. reduced disease severity but a decrease in dry weight was also observed
Efektivitas Ekoenzim Kulit Pisang Kepok Manado Muda sebagai Insektisida Nabati terhadap Hama Penghisap Buah Kakao (Helopeltis sp.) Agustin, Meli; Pratami, Gina Dania; Priyambodo; Agustrina, Rochmah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2025): February 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.9288

Abstract

Kakao merupakan tanaman perkebunan yang mempunyai peran cukup penting dalam perekonomian nasional. Salah satu kendala pada budidaya kakao adalah kehadiran organisme pengganggu tanaman, yaitu kepik penghisap buah kakao (Helopeltis sp.). Pemakaian insektisida kimia secara berlebihan dapat menimbulkan dampak negatif sehingga diperlukan insektisida alternatif yang ramah lingkungan. Ekoenzim kulit pisang kepok manado muda memiliki kandungan bahan aktif yang berpotensi dijadikan insektisida nabati. Penelitian ini bertujuan untuk mengetahui konsentrasi ekoenzim kulit pisang kepok manado muda yang efektif dalam mematikan hama penghisap buah kakao. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) faktorial 2 faktor perlakuan dan 3 ulangan. Faktor pertama adalah konsentrasi ekoenzim (0%, 5%, 10%, 15%, dan 20%). Faktor kedua adalah waktu pengamatan (6, 12, 24, 48, dan 72 jam). Data hasil pengamatan dianalisis menggunakan ANOVA dengan uji lanjut Tukey dan nilai LC50 dianalisis menggunakan probit. Hasil penelitian menunjukkan bahwa ekoenzim kulit pisang kepok manado muda berpotensi sebagai insektisida nabati terhadap hama penghisap buah kakao dengan konsentrasi paling efektif adalah 20%. Sementara hasil analisis probit didapatkan nilai LC50 sebesar 17,95% pada waktu pengamatan 72 jam.
Effects of Magnetic Fields and Metal Ions (Cu, Pb, Al and Fe) on Growth of Anoxygenic Photosynthetic Bacteria (APB) Sumardi; Farisi, Salman; Agustrina, Rochmah; Yunita
Biospecies Vol. 12 No. 2 (2019): Juli 2019
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.961 KB) | DOI: 10.22437/biospecies.v12i2.7595

Abstract

Anoxygenic Photosynthetic Bacteria (APB) has beneficial properties including as probiotic candidates, biofertilizers, bioremediation agents, and can reduce H2S concentrations in waters. However, the APB also has a weakness, slow growth.It is known that the interaction of metal ions and the exposure of magnetic fields increases enzyme activity and growth rates. The aim of this study is to determine the effect of metal ions and the exposure to magnetic fields on APB growth. The APB isolate, AM which is isolated from mangrove roots and tested for its effects on metal ions in seawater complete (SWC) media.The metal ions tested were Cu, Pb, Al and Fe with different concentrations. The metal ion is exposed to a 0.2 mT magnetic field for 10 minutes before being added to the media. The cell count results showed that both metal ions exposed and unexposed to magnetic field did not affect the growth of APB AM isolates. However, the metal ion in SWC media affects the spectra pattern of AM APB isolates.
KARAKTERISTIK DAN POLA SEBARAN PHYTOTELMATA SEBAGAI TEMPAT PERINDUKAN ALAMI NYAMUK DI KEBUN RAYA LIWA LAMPUNG BARAT Sitinjak, Mentoria Alviani; Rosa, Emantis; Agustrina, Rochmah; Sutyarso
Biospecies Vol. 13 No. 2 (2020): Juli 2020
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v13i2.9540

Abstract

Liwa Botanical Garden (KRL) is an ex-situ plant conservation area which has a large collection of plants. Therefore, there will be many types of plants in KRL that have phytotelmata. Phytotelmata is a plant in which the organs can hold water and is used by various organisms including mosquitoes as natural breeding grounds. The aim of this study was to determine the phytotelmata characteristics and phytotelmata distribution patterns in the Liwa Botanical Garden, West Lampung. This research was carried out in November 2019 - January 2020 at the Liwa Botanical Garden and the University of Lampung Botanical Gardens. The study was conducted descriptively with a survey method approach. The data obtained is displayed in tables and figures. The results showed that there were 7 types of phytotelmata in the Liwa Botanical Garden namely phytothelmata in the plants of Nepenthes mirabilis, Cyathea coopei, Bambusa sp. Areca catechu, Musa sp. Colocasia gigantea, and Crynum asiaticum with 2 phytotelmata distribution patterns: in groups with IP values ranging from 0.813-1,194 and uniform distribution patterns with IP values of -2.08.Keywords: Phytotelmata, Natural Breeding Ground, Kebun Raya Liwa.
Pertumbuhan Ipomoea reptans Poir. dengan Aplikasi The Kompos Serat Bromelain Terinduksi Trichoderma sp. dan Aspergillus sp. Firda Safira, Masnoni; Irawan, Bambang; Ekowati, Christina Nugroho; Agustrina, Rochmah
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 2 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.2.169

Abstract

Bromelain fiber is the product of extracting the enzyme bromelain from pineapple hulls or peels. Bromelain fiber contains lignocellulose, which, when fully decomposed, is very beneficial for the environment, but it is quite challenging to degrade naturally. Hence, it requires a decomposer agent. The decomposer agents used in this study were cellulolytic fungi Aspergillus sp. (Bioggp 3) and ligninolytic Trichoderma sp. (Bioggp 2). This study aims to determine the effect of compost tea (CT) bromelain fiber induced by these fungi and obtain the best dose of the CT on the growth of land kale (Ipomoea reptans Poir.). This research was conducted in April–July 2022 at the Microbiology Laboratory of FMIPA Unila. This study used a completely randomized design (CRD) with 7 treatments and 4 replications, namely P0 (control), P1 (ACT 25%), P2 (NACT 25%), P3 (ACT 50%), P4 (NACT 50%), P5 (ACT 75%), P5 (NACT 75%). The parameters measured were plant height and number of leaves. The data were analyzed using ANOVA α = 5% followed by the Least Significant Difference (LSD) test with a 5% confidence interval. This study showed that the results with the highest height value and number of leaves were found in P3 (ACT 50%) while the lowest was in P0 (water control). It can be concluded that the ACT and NACT significantly affect the height and number of leaves, and the best dose was found in ACT 50% starting from day 10, 15 , 20, and 25 after planting. Keywords: Aspergillus sp., bromelain fiber, compost tea, Ipomoea reptans Poir, Trichoderma sp.
ANALISIS KANDUNGAN KARBOHIDRAT TERLARUT TOTAL PLANLET BUNCIS (Phaseolus vulgaris L.) MENGGUNAKAN METODE FENOL-SULFUR SECARA IN VITRO Nurcahyani, Endang; Mutmainah, Nurul Aniqotun; Farisi, Salman; Agustrina, Rochmah
Analit : Analytical and Environmental Chemistry Vol. 4, No. 01 April (2019) Analit : Analytical and Environmental Chemistry
Publisher : Jurusan Kimia FMIPA Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/aec.v4i1.2019.p73-80

Abstract

Buncis merupakan salah satu jenis kacang-kacangan yang cukup populer dikalangan masyarakat karena memiliki kandungan gizi yang tinggi dan memiliki potensi nilai ekonomi-sosial yang baik serta berdaya guna untuk mempertahankan kesuburan tanah. Rhizoctonia solanii merupakan salah satu mikoriza yang dapat dimanfaatkan untuk membantu meningkatkan proses penyerapan air tanah pada saat tanaman sedang mengalami cekaman kekeringan. Penelitian ini bertujuan untuk mengetahui efek pemberian Rhizoctonia solanii pada kandungan karbohidrat planlet buncis (Phaseolus vulgaris L.) dalam kondisi cekaman kekeringan secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap Faktorial dengan 2 faktor, yaitu inokulasi Rhizoctonia dengan 2 taraf [V0 (tidak diinokulasi Rhizoctonia) dan V1 (diinokulasikan Rhizoctonia)] dan konsentrasi Poly Ethylene Glycol (PEG 6000) dengan 3 taraf [P0 (0 %), P1 (15 %) dan P2 (30 %)]. Data dianalisis dengan menggunakan ANOVA dan uji lanjut dengan Beda Nyata Terkecil (BNT) pada taraf nyata 5 %. Masing-masing konsentrasi dilakukan 4 kali ulangan dan setiap ulangan terdiri dari 3 biji buncis dalam setiap botol kultur. Hasil penelitian menunjukkan bahwa inokulasi mikoriza Rhizoctonia solanii belum memberikan pengaruh terhadap kandungan karbohidrat terlarut total planlet buncis dalam kondisi cekaman kekeringan secara in vitro.doi: http://dx.doi.org/10.23960/aec.v4.i1.2019.p73-80 
EFEK INOKULASI Rhizoctonia solanii TERHADAP KANDUNGAN KARBOHIDRAT TERLARUT TOTAL PLANLET KACANG PANJANG (Vigna unguiculata (L.) Walp.) SECARA IN VITRO Nurcahyani, Endang; Sazilly, Muhamad Rizkci; Farisi, Salman; Agustrina, Rochmah
Analit : Analytical and Environmental Chemistry Vol. 4, No. 01 April (2019) Analit : Analytical and Environmental Chemistry
Publisher : Jurusan Kimia FMIPA Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/aec.v4i1.2019.p81-90

Abstract

Kendala yang dihadapi dalam memproduksi kacang panjang antara lain musim kemarau yang berkepanjangan. Mikoriza Rhizoctonia solanii dapat membantu menyerap unsur hara dan air bagi tumbuhan. Poly Ethylene Glykol (PEG 6000) diberikan pada medium kultur jaringan untuk mensimulasi cekaman kekeringan. Penelitian ini bertujuan untuk mengetahui efek inokulasi Rhizoctonia solanii terhadap kandungan karbohidrat terlarut total planlet kacang panjang yang diberi PEG 6000 secara in vitro. Penelitian ini dilakukan menggunakan pola dasar Rancangan Acak Lengkap Faktorial (RALF) yang terdiri dari 2 faktor yaitu faktor V; tanpa inokulasi Rhizoctonia solanii/Kontrol (V0), dan Inokukasi (V1), faktor P; PEG 0 % Kontrol (P1), 15 % (P2), 30 % (P3). Penelitian ini dilakukan dengan 4 pengulangan. Parameter yang diamati pada penelitian ini yaitu kandungan karbohidrat terlarut total. Data yang diperoleh di homogenkan menggunakan uji Levene, kemudian dilanjutkan uji ANOVA pada taraf nyata 5%, jika signifikan maka dilakukan uji Lanjut pada BNJ taraf 5%. Hasil penelitian menunjukkan bahwa inokulasi mikoriza Rhizoctonia solanii tidak berpengaruh nyata pada kandungan karbohidrat terlarut total, sedangkan PEG 6000 tidak berpengaruh nyata pada kandungan karbohidrat terlarut total. Berdasarkan hasil penelitian dapat disimpulkan bahwa inokulasi pada akar mikoriza Rhizoctonia solanii tidak memberikan pengaruh pada kandungan karbohidrat terlarut total planlet kacang panjang terhadap cekaman kekeringan.doi: http://dx.doi.org/10.23960/aec.v4.i1.2019.p81-90 
Pengaruh Perendaman Benih dalam Ekstrak Etanol Daun Rumput Teki (Cyperus rotundus L.) Terhadap Pertumbuhan Cabai Merah Besar (Capsicum annuum L.) Azzahra, Muthia; Yuianty, Yulianty; Chrisnawati, Lili; Agustrina, Rochmah
Jurnal Penelitian Inovatif Vol 4 No 3 (2024): JUPIN Agustus 2024
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/jupin.465

Abstract

Cabai merah besar (Capsicum annuum L.) merupakan salah satu tanaman hortikultura yang memiliki banyak manfaat. Peran dan manfaatnya tersebut menjadikannya sebagai salah satu komoditas hortikultura dengan tingkat produktivitas yang tinggi. Produksi cabai merah besar (C. annuum L.) cenderung terus menurun dan salah satu penyebabnya yaitu gulma. Gulma yang biasa hidup berdampingan dengan tanaman hortikultura ialah rumput teki (Cyperus rotundus L.). Rumput teki mengandung senyawa alelokimia yang akan mempengaruhi tanaman disekitarnya. Senyawa alelokimia tersebut dapat memacu sekaligus menghambat pertumbuhan tanaman. Oleh karena itu dilakukan uji perendaman benih C. annuum L. di dalam ekstrak etanol daun C. rutundus L. untuk mengetahui pengaruhnya terhadap pertumbuhan C.annuum L. Penelitian ini dilakukan menggunakan Rancangan Acak Lengkap (RAL) dengan ekstrak etanol daun rumput teki (C. rotundus L.)  dalam 6 taraf konsentrasi (0%, 5%, 10%, 15%, 20%, dan 25%) dengan 4 kali pengulangan.  Parameter yang diamati yaitu persentase perkecambahan, tinggi tanaman, jumlah daun, panjang akar, berat basah tanaman, berat kering tanaman, kandungan klorofil dan skor keracunan. Data yang diperoleh dianalisis dengan ANOVA dan dilanjutkan uji lanjut Beda Nyata Jujur (BNJ) dengan taraf 5 % (α= 5 %). Hasil penelitian menunjukkan bahwa ekstrak rumput teki pada konsentrasi 5% dapat memacu pertumbuhan jumlah daun, menghasilkan jumlah berat basah dan berat kering terbesar pada tanaman cabai merah besar, sedangkan konsentrasi 25% dapat menghambat proses perkecambahan, menekan pertumbuhan tinggi tanaman, menekan jumlah kandungan klorofil dan menyebabkan keracunan pada daun.
Antibacterial Activity Test of Ethanol Extract of Fiddlehead (Diplazium esculentum) Agains the Growth of Staphylococcus aureus and Escherichia coli Bacteria in Vitro: Uji Aktivitas Antibakteri Ekstrak Etanol Pakis Sayur (Diplazium esculentum) terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Escherichia coli secara in Vitro Saragih, Hana Dipa Christine; Agustrina, Rochmah; Farisi, Salman; Sumardi
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 2 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Infectious diseases are a significant public health issue in both developed and developing countries. Infectious diseases are caused by the bacteria Staphylococcus aureus and Escherichia coli, which cause high rates of morbidity and mortality. Treatment of infectious diseases caused by pathogenic bacteria generally uses antibiotics, but the continuous use of antibiotics can have side effects such as the occurrence of antibiotic resistance.  Diplazium esculentum is one of the medicinal plants that is often used to treat infectious diseases.  The content of secondary metabolite compounds contained in D. esculentum includes saponins, steroids, alkaloids, tannins, glycosides, triterpenoids, phenols, flavones, and flavonoids.  This study aims to determine the effect of bacteriostatic activity of ethanol extract of D. esculentum and its effective concentration in inhibiting the growth of pathogens S. aureus and E. coli.  The study was conducted using a Randomised Complete Block Design (RAKL) with four concentrations of ethanol extract of vegetable fern, namely 20%, 33%, 43%, and 50%.  As a positive control, the antibiotic chloramphenicol was used and the negative control used sterile distilled water.  Data in the form of inhibition zone diameter formed from each treatment were analysed using Kruskal Wallis non-parametric test and continued with Mann Whitney Post Hoc test.  The results of the study showed that the concentration of ethanol extract of D. esculentum showed bacteriostatic activity against S. aureus bacteria at concentrations of 43% and 50% with inhibition zones of 6.52 mm and 5.55 mm, as well as E. coli bacteria at concentrations of 43% and 50% with inhibition zones of 5.11 mm and 4.37 mm, but was not effective in inhibiting the growth of S. aureus and E. coli bacteria.
Co-Authors A.A. Ketut Agung Cahyawan W Abda Abda Agustin, Meli Ajeng Pratiwi Azzahra, Muthia Bambang Irawan Christina Nugroho Ekowati, Christina Nugroho Dania Pratami, Gina DEWI SARTIKA Endang Nurcahyani Erika Erika Ernawiati, Eti Fadhilla, Nurul Fardhira, Zikra Farisi, Salman Firda Safira, Masnoni Fithria Mumtazah, Dzul Gina Dania Pratami, Gina Dania istiqomah istiqomah Kusuma Handayani, Kusuma Lili Chrisnawati, Lili Madi Hartono Maemonah, Maemonah Mahfut Mohammad Kanedi, Mohammad Mulyono Mulyono Mumtazah, Dzul Fithria Musa Mutmainah, Nurul Aniqotun Novitasari, Vina Nuning Nurcahyani Nur Qolbi, Hana Pramana, Yanuar Sigit Pratiwi, Rahayu Fathanah Priyambodo Priyambodo Priyambodo Priyambodo Priyambodo Putra, Rizani Oktanisyah Putri, Desma Ramadhina Putri, Jean Delliana Rosa, Emantis RR. Ella Evrita Hestiandari Sabana, Amaliya Saragih, Hana Dipa Christine Sazilly, Muhamad Rizkci Sembiring, Rinawati Setiawati, Primasari Linda Sitinjak, Mentoria Alviani Sri Yusnaini Sumardi Sumardi . Supriyanti, Arni Sutyarso Tamara, Derlian Ella Tengku Riza Zarzani N Tugiyono Tugiyono Tundjung Tripeni Handayani, Tundjung Tripeni Yana, Dellya Vivi Yuianty, Yulianty Yulianty Yulianty YUNITA