Sintho Wahyuning Ardie
Department Of Agronomy And Horticulture, Faculty Of Agriculture, IPB University, Dramaga Campus, Bogor 16680, Indonesia

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Journal : Journal of Tropical Crop Science

Improvement of Shelf Life and Sensory Quality of Pummelos by Fruit Waxing and Wrapping Aji, Titistyas Gusti; Susanto, Slamet; Sukma, Dewi; Ardie, Sintho Wahyuning
Journal of Tropical Crop Science Vol 4 No 1 (2017): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (197.349 KB) | DOI: 10.29244/jtcs.4.1.13-20

Abstract

The aims of this study were to evaluate the physical and chemical change of pummelo fruit during storage with waxing and wrapping, and to determine the effect of waxing and wrapping and their  its interaction in affecting shelf life of pummelo fruits. This research was arranged in a randomized complete block design with two factors and three replications. The first factor was waxing consisted of without waxing, 10% beeswax, 5% beeswax, and chitosan. The second factor was wrapping consisted of without wrapping and wrapping using either transparent yellow or white plastic wrap. Pummelo fruits experience weight loss, reduced total soluble solid (TSS), decrease in firmness and total acidity during 48 days of storage in room temperature. Waxing using beeswax or wrapping using plastic wraps significantly suppressed weight loss and maintained fruit firmness compared to those without waxing and wrapping. However, waxing and wrapping did not significantly affect the quality of pummelo fruits. Based on the fruit’s weight loss, firmness, and panelist preferences, 5% or 10% bees wax can be used as waxing. Both transparent yellow and white plastic wraps can be used to maintain fruit weight, firmness, and appearances during storage. 
Biodiversity Assessment of Foxtail Millet (Setaria italica L.) Genotypes Based on RAPD Marker ARDIE, Sintho Wahyuning; Khumaida, Nurul; Fauziah, Nurul; Yudiansyah, Yudiansyah
Journal of Tropical Crop Science Vol 4 No 1 (2017): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1155.767 KB) | DOI: 10.29244/jtcs.4.1.21-25

Abstract

Foxtail millet (Setaria italica L.) is an important crop in areas where harsh environmental conditions limit crop productivity, including in high salinity and drought prone areas. In Indonesia millet is cultivated in certain areas; however, superior varieties are less developed in the country. The objective of this study was to analyze the genetic diversity among foxtail genotypes using RAPD markers. Genomic DNA of ten foxtail millet genotypes was amplified using 26 random primers through RAPD analysis. Of these primers, 22 produced reproducible amplicons and were polymorphic among the 10 foxtail millet genotypes. The number of polymorphic markers for each primer varied from 1 (primer E15) to 14 (primer M17). The amplified product size ranged from 120 to 2500 base pairs (bp). A dendrogram constructed based on the UPGMA clustering method put all genotypes in 5 distinct groups at 0.64 coefficient level. Diverse genotypes identified in this study can be used as potential parents in an efficient crop improvement program.
Nonparametric Stability Analysis of Starch Content of Gamma Irradiated Cassava at Three Locations in West Java, Indonesia Agustina, Ferra Anggita; Khumaida, Nurul; Ardie, Sintho Wahyuning; Syukur, Muhamad
Journal of Tropical Crop Science Vol 7 No 02 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (647.771 KB) | DOI: 10.29244/jtcs.7.02.66-74

Abstract

Cassava is one of the largest starch producing tuber crops in Indonesia. Tapioca from cassava starch can be used as a raw materials for a variety of foods including biscuits, instant porridge, meatball, sausage, nuggets, and flour condiment. Breeding programs to improve cassava yield and starch content by using mutagen gamma rays irradiation was tested in four cassava generations (M1V4). Cassava is propagated by stem cuttings and the new characteristic obtained from gamma irradiation mutation is stable and can be passed from one generation to the next. Cassava mutants were obtained by evaluating the performance in different environmental conditions. The testing of yield adaptability and stability through a series of multi location is an important step before a new variety can be released. The aim of this research was to compare nonparametric stability and to evaluate the stability of the starch content of 16 genotypes (14 mutants and 2 varieties) in three locations in West Java Province i.e. Tapos (Depok), Cikarawang (Bogor), and Ciseeng (Bogor). Experiments were conducted in a completely randomized block design with three replications nested in each environment. Testing of yield stability in this study used four approaches with 10 parameters of nonparametric method. Starch content was estimated using gravimetric method. Based on frequency stability ranking of starch content from gravimetric method the G63142 genotype had the highest starch content estimation (29.99%), and the top five genotypes with high starch content were G63142, G61142, “Manggu”, G62151, and G63124. G61142 was categorized as genotype with static and dynamic stability, therefore this genotype is a very potential mutant to be released, whereas G63124 is categorized as a genotype with a static stability. Genotypes G63142, G62151, and “Manggu” had variable but relatively high starch content.
Evaluating the Efficiency of Metsulfuron-Methyl: The Role of Rice Cultivation Practices in Controlling Monochoria vaginalis Kansa Dianti Putri; Guntoro, Dwi; Sintho Wahyuning Ardie; Hariyadi
Journal of Tropical Crop Science Vol. 11 No. 02 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.02.175-183

Abstract

Monochoria vaginalis is an invasive weed that thrives in rice paddies across Indonesia. Herbicide control is challenging since Monochoria vaginalis is known to be sulfonylurea-resistant in Indonesian rice fields. This weed is highly competitive, with a rapid growth pattern that may diminish rice production. Metsulfuron-methyl, a widely used herbicide, can potentially eliminate sensitive weeds at low dosage levels. This study aimed to identify the optimal dosage of metsulfuron-methyl for controlling Monochoria vaginalis and assess the impact of monoculture rice growing patterns and herbicide application in controlling weeds. The experimental design was a split-plot design with four replications, conducted from June to July 2023 in the Cikabayan Greenhouse at IPB University. The major plot was the Monochoria vaginalis accession: exposed or not exposed to herbicide, consisting of seven sites. The subplot represented the dosage of the active ingredient (a.i.) metsulfuron-methyl: 0, 1, 2, 4, 8, 16, and 32 g.ha-1. In addition to the field experiments, interviews were performed with five farmers from each of the seven Monochoria vaginalis sample accessions to assess rice planting patterns and herbicide usage history. The results showed that metsulfuron-methyl at 1 g.ha-1 reduced weed populations by over 50% in rice accessions Bugel, Palumbonsari, and Ciasem Hilir. Variability in Monochoria vaginalis indicated resistance to metsulfuron-methyl, necessitating greater dosages up to 8 g a.i. per ha-1 for Pasirkaliki and Rancajaya accessions. Our study also indicated that some areas could use lower dosages of herbicides to effectively control Monochoria vaginalis. In contrast to other places that required larger, three-yearly dosages of 100 to 125 g.ha-1, Bugel, Palumbonsari, and Ciasem Hilir used a twice-yearly dosage of 20 g.ha-1. These findings highlight the importance of tailored herbicide application strategies based on local resistance patterns and historical herbicide use to manage Monochoria vaginalis effectively.
Genetic Diversity of Yellow and Red Berries Arabica Coffee Populations Grown in a Mix Populations in Garut, West Java, Indonesia, Based on SSR Markers Cahyono, Andy Agus; Ardie, Sintho Wahyuning; Rubiyo, Rubiyo; Sudarsono, Sudarsono
Journal of Tropical Crop Science Vol. 11 No. 03 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.03.207-216

Abstract

Farmers in Garut, West Java, grow mixed varieties of Arabica coffee (Coffea arabica L.). Subsequently, they use harvested beans as seeds. Intercrossing among varieties may result in hybrid progenies and harvesting hybrid progenies as seed results in genetic variations. This research aims to evaluate the genetic diversity of Arabica coffee grown in a mixed population. Ninety-one Arabica coffees comprised 37 Arabica cv. “Ahernt GRT KN” (yellow-), 45 “Sigararutang” (red-), and nine “S795” (red berries) were sampled. Twenty SSR primer pairs were validated using 15 samples representing three varieties; six were polymorphic and used to genotype 91 Arabica accessions. Genetic data were analyzed using PowerMaker 3.25 and Darwin version 6 software. The results showed that the six SSR loci generated from 2 – 3 alleles, with an average of 2.17 alleles per locus. Genetic analysis of Arabica coffee from Garut, West Java, generated SSR markers with an average PIC of 0,33 across loci and varieties. The PIC within Arabica coffee cv. “Ahernt GRT KN” and “Sigararutang” were low, and within “S795” was moderate. Those PICs indicate the presence of more genetic diversity within “S795” than the other two cultivars. The Ho across Arabica coffee cv. “Sigararutang” and “S795” were lower than the He values, confirming their self-pollination nature. However, the Ho values of Arabica coffee cv. “Ahernt GRT KN” was larger than the others, indicating the presence of residual heterozygosity and a low percentage of recent outcrossing. The low Ho values of “Sigararutang” suggest that Arabica coffee is homozygous. Arabica coffee cv. “S795” also showed a low Ho value, but its moderate He value indicates the presence of more genetic diversity than the othercultivars.
Genetic Variability of Echinochloa crus-galli Ecotypes from West Java, Indonesia, by RAPD Analysis Pasaribu, Pesta Maria Hotnauli; Rizqullah, Ramadaniarto; Ardie, Sintho Wahyuning; Guntoro, Dwi
Journal of Tropical Crop Science Vol. 12 No. 01 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.01.18-24

Abstract

Echinochloa crus-galli (Barnyardgrass: Poaceae), is one of the most detrimental weeds in rice fields globally. As one of the leading rice producers in Indonesia, controlling barnyard grass in rice fields in West Java province is of great importance. Information on the genetic variability of barnyard grass is necessary to determine proper weed control. A molecular marker is considered the most accurate tool in determining genetic variability as its profile is unaffected by the environment. The objective of this study was to evaluate the genetic variability of barnyard grass collected from seven sub-districts in West Java province, Indonesia, using RAPD markers. Genomic DNA of barnyard grass ecotypes from “Bayusari”, “Majalaya”, “Klari”, “Cugenang”, “Cianjur”, “Ciomas”, and “Ciampea” sub-districts were analyzed using eight RAPD primers and resulted in a total of 87 reproducible amplicons. Of these amplicons, 59 were polymorphic, and 28 were monomorphic, with a polymorphism percentage ranging from 37.5-92.8%. Polymorphism information content (PIC) values ranged from 0.21 to 0.41, indicating the used RAPD markers are highly informative. All seven ecotypes were divided into three distinct groups with a coefficient level of 0.77 in a dendrogram constructed following the UPGMA clustering method. Group 1 consisted only of the “Bayusari” ecotype. Group 2 consisted of “Majalaya”, “Klari”, and “Cugenang” ecotypes, while Group 3 consisted of “Cianjur”, “Ciomas”, and “Ciampea” ecotypes. This research indicated remote dispersal of E. crus-galli, since ecotypes from distant locations were found to be closely related
Grafting-Induced Phenotypic and Genomic Variations in Cassava (Manihot esculenta Crantz) through Interspecific Compatibility with M. Glaziovi Ulfa, Umi Tahniah; Sudarsono, Sudarsono; Ardie, Sintho Wahyuning; Mulyaningsih, Enung Sri; Hartati, N. Sri; Anjarsari, Nurul Aeni; Dewi, Prita Sari
Journal of Tropical Crop Science Vol. 12 No. 03 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.03.559-571

Abstract

Grafting is a promising approach to enhance cassava (Manihot esculenta Crantz) productivity, particularly in improving tuber yield and quality. Despite its technical simplicity, research on the morphological and molecular consequences of grafting in cassava is limited. This study aimed to quantitatively assess phenotypic differences between grafted and shoot cutting plants and characterize genetic modifications in shoot apical meristems near the graft union. Three splice-grafting combinations were evaluated: M. glaziovii (cv. "Karet")/"Revita RV1" (KR), "Karet"/ "Carvita 25" (KC), and "Carvita 25"/ "Revita RV1" (CR), alongside shoot-cutting plants of each cultivar. Grafted seedlings and conventional cuttings were transplanted to the field, and growth was monitored up to five months after grafting (5 MAG). Shoot bud samples were collected for RAPD analysis at three months (3 MAG). Grafted plants showed differences in morphology compared to controls, especially in branching architecture, branch angle, stem diameter, and internode length. However, the overall plant form and primary stem type remained stable. The color of emerging shoots consistently reflected their genotypic origin, like scion or rootstock, as did the shoot buds nearest the graft union. RAPD analysis using multiple primers revealed polymorphic banding patterns, with OPE-12 producing the most diverse profiles. While specific bands were associated with each cultivar, grafted regenerants often exhibited altered or novel band profiles, suggesting somaclonal variation or graft-induced genetic/epigenetic changes. Some regenerants displayed unexpected band types, such as C1 in R-KR1 and R-KR3, which were absent from their known parental genotypes. Cluster analysis grouped 45 samples into five major clusters and eight subgroups, indicating genetic divergence among certain grafted combinations, particularly "Carvita 25"–"Revita RV1" and M. glaziovii–"Revita RV1". These results demonstrate that grafting can significantly impact cassava phenotype and genome stability. This study shows that interspecific grafting between cassava (Manihot esculenta Crantz) and M. glaziovii can induce significant phenotypic and genomic variations.
Pests and Diseases in Foxtail Millet (Setaria italica L. Beauv.) Cultivated in The Greenhouse Khairani, Hagia Sophia; Nurulalia, Lia; Ardie, Sintho Wahyuning
Journal of Tropical Crop Science Vol. 8 No. 03 (2021): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.8.03.146-153

Abstract

Greenhouse ecosystem with more stable abiotic factors could affect the population and diversity of pests and diseases found on foxtail millet (Setaria italica L. Beauv.) compared to their natural ecosystems. We observed and identified pests and diseases in seven genotypes of foxtail millet namely “Toraja”, “ICERI 5”, “ICERI 6”, “Botok 4”, “Botok 10”, “Mauliru”, and “Hambapraing”; these activities are important for the formulation of appropriate integrated pest management techniques. Using plant samples that were at the end of their vegetative phase, we found white mycelia of Fusarium incarnatum (yellowish-white colonies) and F. verticilloides (violetish-pink colonies) covering the ear-tip of the seeds and developed rapidly leading to seed rotten symptoms in “Toraja”, “ICERI 5”, and “ICERI 6”. The disease severity remained constant after these integrated management techniques were put in place. However, abnormalities in leaves leading to failure of panicle emergence occurred in “Botok 4”, “Botok 10”, “Mauliru”, and “Hambapraing”. These were caused by the fungi Penicillium sp. Identical controlling techniques were applied to this incidence and the disease incidence was reduced. Corn leaf aphids (Rhophalosiphum maidis) and rice mealybugs (Brevenia rehi) were recorded as main insect pests with severe attack. The aphid, R. maidis, colonized the stems and were associated with the sooty mold (Capnodium sp.), resulting in wilting. The mealybugs, B. rehi, colonized the flag leaves resulting in leaf rotting. Insecticide and isolating the attacked plants were used as the controlling techniques. Red-mites (Tetranychus urticae) were also detected as indicated by chlorotic spots on the upper part of the leaves. Acaricide was used to reduce its population. The pests and diseases found in the seven genotypes of foxtail millet are commonly known to occur in Poaceae. In general, greenhouse ecosystem for foxtail millet facilitated planting with high population with lower diversity of pests and diseases compared to the open field planting.
Harvesting Criteria and Drying Methods to Improve the Quality of Foxtail Millet Seeds (Setaria italica L. Beauv.) Lainufar, Putri Aulia; Qadir, Abdul; Suhartanto, M. Rahmad; Ardie, Sintho Wahyuning
Journal of Tropical Crop Science Vol. 8 No. 03 (2021): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.8.03.168-176

Abstract

Foxtail millet is annual grasses with grains that are smaller than those of sorghum, rice, and wheat, and is considered one of the minor economic crops but with nutritional values similar to other major food crops. The development of millet as major cereal crop is considered challenging due to the low quality of its seeds, and studies have been conducted to improve millet’s seed quality. We conducted this study to determine the harvesting criteria of foxtail millet seeds based on the change in color of the panicles. We also wanted to determine the drying treatment, and evaluate the relationship between the position of the seed on the panicles and the seed viability and vigor. The first experiment was arranged based on a completely randomized design with panicle color as the first factor (green, yellow 75%, and brown) and drying time as the second factor (0 hours, 24 hours, 48 hours, 72 hours). The second experiment was arranged in a completely randomized design with seed position as the main factor (base, middle, tip). The study was conducted on two genotypes of millet, i.e. “BOTOK 4” and “BOTOK 10”. The highest seed quality of “BOTOK 4” and “BOTOK 10” genotypes were obtained when the panicles were brown and dried for 72 hours; the seed chlorophyll content was the lowest and water content was 8.88%, with the highest viability and vigor, i.e., germination rate of 88.3%-90%, maximum growth potential of 92%-95.3%, normal sprout dry weight 596-620 mg, vigor index of 33.6% - 21.6%, and growth rate of 18.2%-17.1% etmal-1. The highest seed viability and vigor in “BOTOK 4” genotypes were obtained from the base position, i.e., 78.7% germination rate, maximum growth potential of 83.7%, vigor index of 56.5%, growth speed of 19.6 etmal-1, normal sprout dry weight of 48 mg, radicle length of 3.3 cm) and “BOTOK 10” genotypes from the middle position with 91.5% germination, maximum growth potential of 97.2%, vigor index of 21.7%, growth speed of 17.0% etmal-1, and normal sprout dry weight 61 mg.
Assessment of Yellow and White Fleshed Cassava Tuberous Root Cultivars Reveals Different Responses to Post-harvest Physiological Deterioration Rahmawati, Rika Sri; Fathoni, Ahmad; Sukma, Dewi; Ardie, Sintho Wahyuning; Sudarsono, Sudarsono
Journal of Tropical Crop Science Vol. 11 No. 01 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.01.64-73

Abstract

Identification of post-harvest physiological deterioration (PPD) tolerance in cassava is crucial, as PPD significantly hampers the cassava tuberous root industry by shortening storage periods post-harvest and diminishing product quality. Characteristics linked to PPD tolerance encompass high carotenoid levels and low dry matter content (DMC). This study aimed to evaluate the PPD responses of six yellow fleshed and ten white-fleshed cassava tuberous roots and determine the source of PPD tolerance. PPD and DMC assessments were conducted using standard methods at three storage periods: 2, 5, and 10 days after harvest (DAH). The k-means clustering analysis revealed six clusters, each corresponding to distinct PPD symptom patterns and tolerance statuses. Cluster 1, comprising three yellow-fleshed and three white-fleshed cassava cultivars, demonstrated PPD tolerance with minimal symptoms up to 5 DAH. Clusters 2 and 3 exhibited a moderate PPD response with elevated symptoms at 5 DAH, comprising three yellow-fleshed and three white-fleshed cassava cultivars. Clusters 4 to 6 displayed a sensitive response to PPD, showcasing a significant increase in symptoms at 5 and 10 DAH, with four white-fleshed cassava cultivars identified within this cluster. These findings underscored the presence of PPD tolerance in both yellow-fleshed and white-fleshed cassava tuberous roots. The correlation between PPD and DMC was significant only at 2 DAH, displaying a moderate positive correlation. Consequently, this study identified three cultivars, “Carvita-25”, “Manggu”, and ““Ubi Kuning””, with high DMC and low PPD incidence at 5 DAH, suggesting their suitability for further breeding programs.
Co-Authors , Krisantini Abdul Qadir Agus Zainudin Agustina, Ferra Anggita Ahmad Fathoni Aji, Titistyas Gusti Alex Hartana Anas D Susila Anas Dinurrohman Susila Anjarsari, Nurul Aeni Anshori, Muhammad Fuad Ashan, Muh Aswad Azis Natawijaya Bambang Sapta Purwoko Butarbutar, Lidya Kristina Sari Cahyono, Andy Agus Chusnah, Uswatun Dewi Sukma Didy Sopandie Dwi Guntoro Enny Sudarmonowati Enung Sri Mulyaningsih fatimah Fatimah Ferdinans, Ferdinans Ghulammahdi, Munif Hagia Sophia Khairani HAJRIAL ASWIDINNOOR Hariyadi Hartana, Alex Heni Safitri Hermanasari, Rini Ika Roostika Indrastuti A. Rumanti Irmanida Batubara Ismail maskromo Iswari Saraswati Dewi Iswari Saraswati Dewi Kansa Dianti Putri Karjunita, Nike Lainufar, Putri Aulia Lefin Kafindra Luthfiani, Marisa Vidya M. Syukur Mahrup Mahrup, Mahrup Mashuri Waite maskromo, Ismail Miyamoto, Takuji Mohamad Rahmad Suhartanto Muaz Adbdul Karim Muaz Adbdul Karim Muhamad Syukur Munif Ghulamahdi N. Sri Hartati, N. Sri N. Sri i Hartati Nampiah Sukarno Natawijaya, Azis Nugraha, Yudhistira Nugroho, Rizki Bagus Nurul Fauziah Nurul Khumaida Nurul Khumaida Nurulalia, Lia Pahlevi, M Reza Parlindo, Fitra Pasaribu, Pesta Maria Hotnauli Pratama, Syilvia Nosya Prita Sari Dewi Rahmawati, Rika Sri Rahmi Henda Yani Ramadaniarto Rizqullah Reflinur Resti Putri Septyani Resti Putri Septyani Rika Miftakhul Jannah Rizqullah, Ramadaniarto Romy Agus Saputro Romy Agus Saputro Rubiyo Rubiyo Rumanti, Indrastuti A. Sadewi Maharani, Sadewi Saenal, Nirwanti Safitri, dan Heni Safitri, Vika Ayu Satya Nugroho Satya Nugroho Siswati, Leni Siti Kurniawati Slamet Susanto Soekisman Tjitrosoedirdjo SRI RAHAYU Sri Ratnawati Subekti, Isnani Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono, Sudarsono Suwarno ,, Suwarno Suwarno Suwarno Suwarno, Suwarno Suwarno, Suwarno Syawaluddin, Dwi Dana Syukur, M. TRI JOKO SANTOSO TRI KOESOEMANINGTYAS Tri Lestari Trias Sitaresmi Trikoesoemaningtyas Tsugama, Daisuke Ulfa, Umi Tahniah Umezawa, Toshiaki Vincentia Esti Windiastri, Vincentia Esti Widowati, Sartika Willy Bayuardi Suwarno Yudhistira Nugraha Yudiansyah Yudiansyah Yudiwanti Wahyu E. Kusumo Yuli Irvana Dewi Yuli Sulistyowati Yullianida , Za’aziza Ridha Julia