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All Journal International Journal of Public Health Science (IJPHS) Jurnal Kesehatan Lingkungan indonesia Jurnal Kedokteran Brawijaya Jurnal Kebijakan Kesehatan Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Jurnal Kesehatan Masyarakat Indonesian Journal of Urology The Indonesian Journal of Dental Research Majalah Kedokteran Gigi Indonesia Indonesian Journal of Biotechnology Mutiara Medika: Jurnal Kedokteran dan Kesehatan Biomedika INDONESIAN JOURNAL OF PHARMACY Padjadjaran Journal of Dentistry Oto Rhino Laryngologica Indonesiana INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Multidiciplinary Output Research for Actual and International Issue (Morfai Journal) Journal of Nutrition Science JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Berita Biologi

Indwiani Astuti

Universitas Gadjah Mada

Author-ID : 235973

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Efikasi Fraksi Etanolik Akar Tempuyung (Sonchus arvensis L.) sebagai Kemoterapi Kanker Kolon Berdasarkan Ekspresi Caspase-9 Rohima, Brilliana Nur; Astuti, Indwiani; Ghufron, Muhammad
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 1 (2011)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v11i1.931

Kanker kolon merupakan salah satu kanker banyak dijumpai. Kanker kolon merupakan satu dari 10Â kanker primer paling sering di Indonesia pada 1988, 1989, dan 1991. Berdasarkan perkembangan globalisasi di Indonesia diperkirakan insidensi dan prevalensi kanker kolon akan meningkat. Apigenin adalah bioflavonoid subkelas flavone yang memiliki potensi terapeutik yang besar, salah satunya adalah memacu apoptosis. Apigenin terdapat dalam tempuyung (Soncus arvensis L.) . Tujuan penelitian ini adalah untuk mengetahui potensi fraksi etanolik akar tempuyung (Sonchus arvensis L.)Â untuk kemoterapi kanker kolon melalui ekspresi caspase-9 pada cell line kanker kolon WiDr. Penelitian dilakukan dengan cara sampel tanaman dideterminasi. Akar tempuyung difraksinasi dan dilakukan uji sitotoksisitas, kemudian dilakukan uji imunohistokimia pada cell line kanker kolon WiDr dengan ditambahkan fraksi akar tempuyung Â½ IC50, IC50, dan 2 IC50. Sebagai kontrol negatif digunakan media kultur dan kontrol positif digunakan fluorouracil dosis 46,56 Âµg/mL, kemudian dibuat preparat histopatologi. Hasil penelitian menunjukkan bahwa determinasi spesies sampel adalah Sonchus arvensis L. Hasil uji sitotoksisitas IC50 fraksi etanolik akar tempuyung adalah 2865,5 Âµg/mL. Pada penelitian ini tidak dilakukan uji imunohistokimia karena IC50 fraksi etanolik akar tempuyung terlalu besar (eâ€50 Âµg/mL). Disimpulkan bahwa fraksi etanolik akar tempuyung (Sonchus arvensis L.)Â memiliki potensi yang rendah untuk kemoterapi kanker kolon berdasarkan ekspresi caspase-9 pada cell line kanker kolon WiDr

PREDICTIVE VALUE OF SERUM PROSTATE SPECIFIC ANTIGEN IN DETECTING BONE METASTASIS IN PROSTATE CANCER Danarto, Raden; Astuti, Indwiani; Haryana, Sofia Mubarika
Indonesian Journal of Urology Vol 28 No 2 (2021)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v28i2.570

Objective: We determine the utility of Prostate Specific Antigen (PSA) for predicting the presence of skeletal metastasis on Bone Scan (BS) in prostate cancer patients. Material & Methods: Retrospective analysis of medical records of 70 consecutive prostate cancer patients subjected to bone scan during the last 2 years was done. 5 cases were excluded due to the following reasons: Serum PSA not available, hormonal or other therapy given prior to serum PSA measurement, and/or Bone Scan, and symptomatic for bone metastasis. In remaining 65 cases, PSA value and bone scan were evaluated. Results: BS was found to be positive in 20/65 (31%) and negative in 45(69%) patients. 24 (37%) had serum PSA > 100 ng/ml, 25 (38.5%) had PSA of 20‐100 ng/ml and only 16 (24.5%) had PSA < 20 ng/ml. Conclusion: Serum PSA < 20 ng/ml have high predictive value in ruling out skeletal metastasis. Our data are in corroboration with results from previous studies that BS should be performed only if PSA > 20 ng/ml. Using this cut‐off, unnecessary investigation can be avoided. Avoiding BS asymptomatic in this group of patients would translate into a significant cost‐saving and reduction in their psychological and physical burden.

Effect of Soybean-based Food Supplement on Insulin and Glucose Levels in Type 2 Diabetes Mellitus Patients Purwoko, Akhmad Edy; Astuti, Indwiani; Asdie, Ahmad Husain; Sugiyanto, Sugiyanto
Indonesian Journal of Pharmacy Vol 30 No 3, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Introduction: Indonesia is one of ten countries with the largest diabetic populations in the world. Giving soy supplements to people with diabetes can reduce blood glucose levels significantly. However, no studies have shown a reduction back to normal glucose levels. This study aimed to determine the increase of insulin levels and blood glucose suppression in glucose tolerance tests after supplementation of soy in patients with type 2 diabetes. Sequencing the gene of GLP-1 (37 amino acid). This experimental research was a randomized, treatment controlled; open clinical trial study conducted by comparing the control group treated with soybean supplement products containing 18g protein and placebo. Seventy-six treatment subjects with type 2 diabetes were recruited from Muhammadiyah Gamping Hospital and PERSADIA gymnastics group according to the inclusion and exclusion criteria and then grouped randomly in the supplement treatment and placebo groups. Blood sampling was drawn at 8 hours fasting, 2 hours after supplement administration and 2 hours after administration of 75g glucose for measuring blood insulin and glucose level. Blood insulin level 2 hours after supplement administration (meanÂ±SEM) increased 5.3Â±0.8ÂµIU/ml (n=37) while placebo decreased 0.9Â±0.4ÂµIU/ml (n=39) which was statistically significantly different (p<0.05). Blood glucose levels increased in the glucose tolerance tests, while the supplement group was 130.0Â±11.5mg/dl (n=37) lower than the placebo 146.7Â±8.2mg/dl (n=39) (p>0.05). Gene sequencing shows a nucleotide variation of GLP-1 (37 amino acid) in Javanese T2DM Giving Soybean supplements containing 18g protein increased blood insulin levels and suppressed blood glucose levels in glucose tolerance tests.

Determinants Levels of Urinary 8-Hydroxydeoxyguanosine among Chromium Electroplating Workers Yuliani Setyaningsih; Indwiani Astuti; Adi Heru Husodo
International Journal of Public Health Science (IJPHS) Vol 5, No 4: December 2016
Publisher : Intelektual Pustaka Media Utama

Electroplating workers were exposed by particulate and fog of chromium from electrolysis process during work. Chromium is highly carcinogenic when inhaled. Chromium can be reduced in the cells in the body. This process may generate Reactive Oxygen Species (ROS) causing lipid peroxidation and oxidative DNA damage. This research was aimed to analyse the influence of chromium to the levels of urinary 8-OHdG as an indicator to support early diagnosis of occupational disease. 66 electroplating workers in Tegal District were taken purposively as samples for this research. There were association between chromium level in urine, malondialdehyde (MDA) level, Body Mass Index, smoking habit, alcohol and supplement drink consumption and urinary 8-OHdG (p<0.05). There was not association between BMI and urinary 8-OHdG (p>0.05). Multivariate analysis using logistic regression showed the alcohol consumption had the most powerful influence to the level of urinary 8-OHdG. The levels of urinary 8-OHdG could be used as an indicator of oxidative DNA damage and early diagnosis of occupational disease among electroplating workers who were exposed by chromium.

MiR-141-3p Relative Expression Level from FFPE Samples as Biomarker of Prostate Adenocarcinoma Carcinogenesis in Yogyakarta, Indonesia Sari Eka Pratiwi; Sri Nuryani Wahyuningrum; Rachmagreta Perdana Putri; Danarto Danarto; Didik Setyo Heriyanto; Nur Arfian; Sofia Mubarika Haryana; Indwiani Astuti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 1 (2022): The future of diagnostic laboratory testing
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i1.2355

Globally, prostate cancer (PCA) is the second leading cause of male cancer-associated mortality. Micro-RNAs (miRNAs) are small non-coding RNAs considered promising biomarkers for diagnosis, prognosis, and treatment options. A miR-141 expression is frequently dysregulated and influences the development and progression of PCA. This study aimed to identify miR-141 expression level as a marker to differentiate PCA from another prostate anomaly, especially in Yogyakarta. Formalin-fixed paraffin-embedded (FFPE) tissues for each three groups: benign prostatic hyperplasia/BPH, high-grade prostatic intraepithelial neoplasia/HGPIN, and PCA (n=7/group) were stored in a commercial clinical laboratory in Yogyakarta. The total RNA was extracted from FFPE sections using miRNeasy FFPE kit, followed by the quantification of miR-141-3p expression level by RT-PCR. The result showed that miR-141 relative expression level on PCA was higher than other groups and significantly different (P<0.05, Kruskal Wallis test). The mean of the miR-141 relative expression level of BPH, HGPIN, and PCA were 1.04±0.87, 6.44±7.8, and 7.06±8.83, respectively. The relative expression level of miR-141 can potentially be a prognostic biomarker in PCA and could differentiate aggressiveness in prostate anomaly, especially BPH, HGPIN, and PCA.

Systemic IL-1β and TNF-α Productions of E. coli Lipopolysaccharide-Induced Periodontitis Model on Rats Alma Linggar Jonarta; Widya Asmara; Indwiani Astuti; Regina TC Tandelilin
The Indonesian Journal of Dental Research Vol 1, No 1 (2010)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Periodontal disease, a common inflammatory oral disease involved periodontal tissues, has been linked with the evidence of some systemic disorders. Recently, periodontal disease has been suspected as a trigger of systemic disorders. Penetration of bacterial products, such as lipopolysaccharide (LPS) may reach into deeper periodontal tissues. Therefore there may affect systemic blood and cytokines production. Interleukin-1β (IL-1β) and Tumour Nuclear Factor-α (TNF-α) are known as pro-inflammatory cytokines. The production of systemic IL-1β and TNF-α of E. coli lipopolysaccharide-induced periodontitis model on rats was investigated in this research. Fifteen male Wistar rats, aged 6-8 weeks used for this study were divided into 3 groups. For group 1 and 2, silk ligature 3/0 were inserted in interdental area between upper right molar 1 and 2. First and second group received solution containing 10μg/ml and 1mg/ml E. coli lipopolysaccharide, respectively, mixedwith 2% carboxymethylcellulose (CMC) diluted in 100μl of phosphate buffer saline (PBS). The solution was topically applied on gingival tissues around the gingival sulcus, a single topical application of solution onceper 2 days for 14 days. Untreated subjects were used as negative control. On day 15, the blood was collected from vena orbitalis, and rats were sacrificed. The blood serum of each group was divided into 2 groups andcultured for 4 hours with or without 20μl of 100ng/ml of E. coli LPS. ELISA techniques were used to measure the cytokine productions of the supernatant. The data was analysed using Repeated Measure ANOVA. This study showed that there was a significant increase of IL-1β production on low dose of LPS compared to control and high dose of LPS groups (p<0.05). Whereas TNF-α not significantly showed increasing trend. The increasing trend of pro-inflammatory cytokine productions, such as IL-1β and TNF-α, on LPS-induced periodontitis model in this experiment supports the previous studies about the contribution of periodontal disease in the pathogenesis of systemic diseases.

Peningkatan Jumlah Mikronukleus pada Mukosa Gingiva Kelinci Setelah Paparan Radiografi Panoramik Rurie Ratna Shantiningsih; Suwaldi Suwaldi; Indwiani Astuti; Munakhir Mudjosemedi
Majalah Kedokteran Gigi Indonesia Vol 20, No 2 (2013): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Mikronukleus merupakan salah satu tanda awal terjadinya kerusakan DNA yang ditemukan pada mukosa gingiva manusia setelah paparan radiografi dental panoramik. Peningkatan jumlah mikronukleus terjadi paling tinggi pada hari ke-10 dan selanjutnya mengalami penurunan sampai dengan hari ke-14. Kelinci memiliki karakter dan periode turn-over mukosa gingiva yang hampir sama dengan manusia berkisar antara 10-12 hari. Tujuan penelitian ini untuk mengevaluasi apakah peningkatan jumlah mikronukleus pada mukosa gingiva kelinci setelah paparan radiografi panoramik. Sembilan ekor kelinci dibagi menjadi 3 kelompok untuk mewakili hari ke-3, 6 dan 9 setelah paparan radiografi panoramik. Sebelum dan sesudah diberikan paparan radiografi panoramik, setiap hewan coba dilakukan apusan pada mukosa gingiva anterior rahang bawah menggunakan cervical brush. Hasil apusan dilakukan pewarnaan dengan modifikasi Feulgen-Rossenbeck dan dihitung jumlah mikronukleus menggunakan mikroskop yang disambungkan dengan optilab. Analisis statistik dilakukan menggunakan paired t-test. Analisis statistik menunjukkan adanya perbedaan yang signifikan (p<0,05) antara jumlah mikronukleus sebelum dan 9 hari sesudah paparan radiografi panoramik. Akan tetapi tidak ditemukan perbedaan yang signifikan (p>0,05) antara sebelum paparan dibandingkan hari ke-3 dan ke-6 setelah paparan radiografi panoramik. Kesimpulang dari hasil penelitian ini sejalan dengan penelitian sebelumnya pada manusia bahwa peningkatan jumlah mikronukleus terjadi pada hari ke-9 setelah paparan radiografi panoramik. Hasil ini mengindikasikan bahwa pada kelinci juga menunjukkan peningkatan jumlah mikronukleus di mukosa gingiva akibat paparan radiografi panoramik.Micronucleus Increase After Panoramic Radiography Exposure In Rabbit’s Gingival Mucosa. Micronucleus is one of the early states of DNA damage found in human gingival mucosa after dental panoramic radiography exposure. The increasing amount of micronucleus will reach a peak in the tenth day after the exposure, and it will continuously decrease right after the fourteenth day. Rabbit has almost the same gingival mucosa and turn-over period with human for about 10-12 days. The purpose of this research is to evaluate the increasing amount of micronucleus in rabbit’s gingival mucosa after panoramic radiography exposure. A total of nine New Zealand rabbits were divided into 3 groups to represent day of 3rd, 6th and 9th after the panoramic radiography exposure. The mandibular anterior gingival mucosa of each animals was swabbed using a cervical brush before and after panoramic radiography exposure. The samples were stained with Feulgen-Rossenbeck modification, and the amount of micronucleus was counted using a microscope that is connected to Optilab. Statistical analysis was performed using paired t-test. The statistical analysis showed that there was significant difference (p <0.05) between the number of micronucleus before exposure and 9th day after panoramic radiography exposure. Moreover, there was no significant difference (p> 0.05) between the amount of micronucleus before exposure compared with 3rd and 6th day after panoramic radiography exposure. Based on the experiment, it is concluded that the result is consistent with previous studies conducted in human that there was increasing amount of micronucleus at the 9th day after panoramic radiography exposure. This result indicates that rabbit performs the increasing amount of micronucleus in gingival mucosa because of panoramic radiography exposure

Polymorphism of Transcription Factor 7-Like 2 Gene and HOMA-β Level of Individuals With and Without Type 2 Diabetes Mellitus Family History Waode Astria Sahrani; Indwiani Astuti; Ahmad Hamim Sadewa
Indonesian Journal of Biotechnology Vol 19, No 2 (2014)
Publisher : Universitas Gadjah Mada

Family history has considered as a risk factor of type 2 diabetes. Transcription factor-7 like 2 (TCF7L2) hasrole to regulates insulin secretion and blood glucose homeostasis. The aim of current study was to determine thers7903146 polymorphism of TCF7L2 gene and homeostatic model assessment-β (HOMA-β) level on individual withand without type 2 Diabetes Mellitus (DM) family history. This work is a case-control study. Thirty six subjectswith type 2 DM family history and 36 subjects without type 2 DM family history were recruited. HOMA-βmeasure to analyze the insulin secretion. Polymorphisms of TCF7L2 gene was analyzed by using PCR-RFLPmethod. Statistical analysis was performed by using T-test, Mann-Whitney and Chi-square with signifi cancelevel 0.05. The frequency of the T allele of the cases were 4.2% and the controls were 2.8% (p=0.500). The oddratio was 0.649 (CI;95%:0.106-4.055). The HOMA-β levels of the cases were signifi cant low (132.56±62.48)compared with the controls (266.09±1.68) with p=0.000. The subjects with type 2 DM family history have asimilar frequency of having T alleles and CT/TT genotypes. The subjects with type 2 DM family history hassignifi cantly lower HOMA-β levels than subject without DM family history.

Kebijakan Penggunaan Batas Wilayah Epidemiologi dalam Pengendalian Penyakit Malaria (Studi Kasus di Puskesmas Kokap II Kabupaten Kulon Progo, DIY) Sutjipto Sutjipto; Hari Kusnanto; Laksono Trisnantoro; Lutfan Lazuardi; Indwiani Astuti
Jurnal Kebijakan Kesehatan Indonesia Vol 4, No 2 (2015)
Publisher : Center for Health Policy and Management

Background: There are 396 endemic districts from the total of 495 districts in Indonesia, with an estimated 45% of the population live in the areas that are at risk of infected malaria disease. Kulon Progo Regency is one of regencies in Yogyakarta (DIY), which until now has not declared elimination of malaria. PHC Kokap II located in Kokap sub-district, is the largest contributor of positive malaria cases in Kulon Progo regency due to the potential for outbreaks of malaria, during the period 1997-2004, when the number of malaria positive patients in the PHC Kokap II ranged between 26% - 55% of patients of the total positive malaria cases in Kulon Progo. This study aims to produce epidemiological information that is important in the region of PHC Kokap II associated with the distribution and determinants of malaria that affects the possibility of local transmission. Methods: The study design was a descriptive study, to get an overview of the distribution and determinants of malaria. The observation unit is the population in the form of correlation studies population and a time series. To determine the pattern of malaria transmission we use secondary data between 2009- 2012 malaria cases. Malaria incidence patterns were analyzed by person, place and time. To determine the clusters of malaria we use clustering analysis with the data of malaria cases in the region PHC Kokap II year 2012. To determine the spread of malaria in the cross-border area between PHC Kokap II and PHC Kaligesing we use secondary data year 2010-2012. Results: In the area of PHC Kokap II, we found import cases every year. Even more, in the year 2009 the proportion of import cases was 82% compared with all patients that were found malaria positive. Clusters of malaria is in the west region of PHC Kokap II, which is bordering to the Subdistrict Kaligesing, Purworejo, Central Java Province. Region PHC Kokap II is an area of high vulnerability, the potential to get the risk of transmission of imported cases of malaria is due to the entry of patients or infective vectors from high transmission areas to low transmission. Conclusion: The focus of malaria transmission in the area of PHC Kokap II is at the western part bordering the sub-district Kaligesing, Purworejo. PHC Kokap II is the region of high vulnerability. Policies need to be made use of epidemiological boundaries in the malaria control program in the cross-border region. Latar belakang: Di Indonesia masih terdapat 396 Kabupaten endemis dari 495 Kabupaten yang ada, dengan perkiraan seki- tar 45% penduduk berdomisili di daerah yang berisiko tertular malaria. Kabupaten Kulon Progo merupakan salah satu kabupa- ten di Daerah Istimewa Yogyakarta (DIY), yang sampai saat ini belum dinyatakan eliminasi. Wilayah Puskesmas Kokap II yang berada di Kecamatan Kokap, merupakan penyumbang terbesar penderita positip malaria untuk wilayah Kabupaten Kulon Progo diantaranya karena potensi terjadinya KLB malaria, selama periode tahun 1997-2004, range jumlah penderita positip ma- laria di wilayah Puskesmas Kokap II berkisar antara 26% - 55% penderita positip malaria di Kabupaten Kulon Progo. Pe- nelitian ini bertujuan untuk menghasilkan informasi epidemiologi yang penting di wilayah Puskesmas Kokap II terkait dengan distribusi dan determinan penyakit malaria yang sangat berpe- ngaruh terhadap kemungkinan terjadinya penularan setempat. Metode: Rancangan penelitian ini adalah studi deskriptif untuk mendapatkan gambaran distribusi dan determinan penyakit malaria, unit pengamatan adalah populasi dalam bentuk studi korelasi populasi dan rangkaian berkala. Untuk mengetahui pola penularan malaria digunakan data sekunder kasus ma- laria tahun 2009-2012, dianalisis pola kejadian malaria menurut orang, tempat dan waktu. Untuk mengetahui kluster penderita malaria dilakukan analisis klustering menggunakan data kasus malaria di wilayah Puskesmas Kokap II tahun 2012. Untuk mengetahui penyebaran penderita malaria di daerah lintas batas antara Puskesmas Kokap II Kabupaten Kulon Progo dan Pus- kesmas Kaligesing Kabupaten Purworejo digunakan data sekunder tahun 2010-2012. Hasil: Di wilayah Puskesmas Kokap II setiap tahun selalu dike- temukan kasus import, bahkan dalam tahun 2009 kasus import proporsinya adalah 82% dibandingkan dengan seluruh pen- derita positif malaria yang diketemukan. Kluster penderita ma- laria berada di bagian barat wilayah Puskesmas Kokap II, yaitu berbatasan langsung dengan Kecamatan Kaligesing Kabupaten Purworejo, Provinsi Jawa Tengah. Wilayah Puskesmas Kokap II merupakan daerah vulnerebilitas tinggi, potensial untuk menda- patkan risiko penularan kasus import karena masuknya pende- rita malaria atau vektor yang infektif dari daerah penularan tinggi ke penularan rendah. Kesimpulan: Fokus penularan penyakit malaria di wilayah Puskesmas Kokap II berada pada wilayah bagian barat berba- tasan dengan wilayah administratif Kecamatan Kaligesing, Ka- bupaten Purworejo. Puskesmas Kokap II adalah wilayah vulnerabilitas tinggi, perlu dibuat kebijakan penggunaan batas wilayah epidemiologi dalam program penanggulangan malaria di wilayah lintas batas.

The expression of multidrug resistance protein 5 and thymydilate synthase on fluorouracil resistance WiDr colon cancer cell line Yolanda Dyah Kartika; Indwiani Astuti; Woro Rukmi Pratiwi
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 7, No 2, (2015)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol7.Iss2.art5

Background: Colorectal cancer becomes one of the most common cancer in Indonesia. Fluorouracil is an essential drug in colorectal cancer therapy as it has been supplemented in about 69% of current regiments of chemotherapy. However, drug resistance has reduced its clinical applications. Many factors that contribute to fluorouracil resistance have been investigated including overexpression of Multidrug Resistance Protein 5 (MRP 5) and high level expression of Thymidylate Synthase (TS). Therefore, the expression of those proteins may become key indicators to predict the presence of resistance in the model of fluorouracil-resistance-WiDr colon cancer cell line. Objective: The aim of this study is to characterize a 5-FU acquired resistance WiDr colon cancer cell line, focus on the expression of MRP5 and TS. Methods: This study was a post test controlled group design. The expression of MRP5 and TS were analyzed using immunocytochemistry method. Before protein expression analysis, 4 groups of WiDr colon cancer cell line, group 1, 2, 3, and 4 (control) were treated with 4 different concentration of fluorouracil 12.3 μM, 6.15 μM, 3.08 μM, and 0 μM respectively. Previous study showed that a significant increased level of IC50 value is observed in group induced with fluorouracil 3.08 μM (group 3). Statistical tests for protein expression performed with SPSS version 19 using Kruskall Wallis with 95% confidence level (p<0.05). Results: The expression of MRP5 and TS in the group developed fluorouracil resistance (group 3) were significantly increased compared to control group (p<0.05). In contrast, the other group exposed with higher dose of fluorouracil did not show both significant increased level of IC50 nor significant different of MRP5 and TS expression compared with the control group. Conclusion: The model of fluorouracil-acquired resistant WiDr cell line expresses Thymidilate Synthase and Multidrug Resistance Protein 5. The expressions of these proteins are in accordance with the profile of fluorouracil resistancy.

Co-Authors Adi Heru Husodo Adi Heru Husodo Adi Heru Sutomo Agus, Zaenal Arifin Nang Ahmad Hamim Sadewa Alma Linggar Jonarta Angga Dwi Prasetyo Anwar, Sufyan Arief Budiyanto Asdie, Ahmad Husain Bambang Hariwiyanto Brilliana Nur Rohima, Brilliana Nur Danarto Danarto Devi Usdiana Rosyidah Dibyo Pramono Didik Setyo Heriyanto Em Sutrisna Enda Silvia Putri Eni Mahawati Erna Kristin Ernawati Ernawati Eva Flourentina Kusumawardani Hajid Rahmadianto Mardihusodo, Hajid Rahmadianto Hari Kusnanto Hermawan, Dessy Husin, Hasanuddin Isadora Gracia, Isadora Itza Mulyani Iwan Dwiprahasto Kadarsih, Sri Laksono Trisnantoro Lorettha Wijaya Lutfan Lazuardi Mae Sri Hartati Wahyuningsih Marniati Marniati Marniati, Marniati Muhammad Ghufron Mulyani, Itza Munakhir Mudjosemedi Mustofa Mustofa Nur Arfian Pratiwi, Woro Rukmi Purwoko, Akhmad Edy Putri, Enda Silvia Putri, Suci Eka R. Danarto, R. Rachma Greta Perdana Putri Raden Danarto Regina TC Tandelilin Rurie Ratna Shantiningsih Sagung Rai Indrasari Sari Eka Pratiwi Sarto Sitarina Widyarini Soenarto Sastrowijoto, Soenarto Sofia Mubarika Haryana Sri Kadarsih Soejono Sri Nuryani Wahyuningrum Suci Eka Putri Sugiyanto, Sugiyanto Sunarti . Sunarti, ' Supriatno Sutjipto Sutjipto Suwaldi . Totok Utoro Waode Astria Sahrani Widya Asmara Yolanda Dyah Kartika Yuliani Setyaningsih Yuni Wijayanti Zainal Arifin Nang Agus

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