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All Journal Jurnal Teknologi Dan Industri Pangan Bionatura Dentika Dental Journal Journal of Dentistry Indonesia The Indonesian Journal of Dental Research ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) Interdental Jurnal Kedokteran Gigi (IJKG) Journal of Functional Food and Nutraceutical Narra J Odonto dental journal Narra X Malacca Pharmaceutics Jurnal of Syiah Kuala Dentistry Society SDJ
Boy Bachtiar
Department Of Oral Biology, Faculty Of Dentistry, Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health

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Isolation and Identification of Indonesian Lactobacillus reuteri strain from Saliva of Young Adults Armelia Sari Widyarman; Stella Pranoto; Citra Fragrantia Theodorea; Endang Winiati Bachtiar; Boy Muchlis Bachtiar
Scientific Dental Journal VOLUME 2, ISSUE 2, MAY 2018
Publisher : Faculty of Dentistry, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/sdj.v2i2.2840

Abstract

Background: Biofilms are involved in a wide variety of microbial infections, including dental caries and periodontitis. The use of probiotics has been a promising prevention and treatment modality with which to combat biofilm-related diseases in the oral cavity. The probiotic Lactobacillus reuteri has been proven to reduce gingivitis and plaque index inside the oral cavity. These bacteria can be found in the digestive system and also in the human oral cavity. Objective: The aim of this study was to identify L. reuteri in the saliva of Indonesian young adults. Methods: Forty saliva samples were collected from 18–24 year-old Indonesian subjects. DNA extraction was performed, and then, the identification of L. reuteri was accomplished using PCR. Six subjects showed positive results. The positive samples were cultured in Rogosa Agar for 24 hours at 37°C anaerobically. Several single colonies were further cultured separately in broth medium before DNA extraction and PCR identification were performed. The four thickest bands were selected for DNA sequencing. Results: An analysis performed using BLAST showed that two of the L. reuteri strains obtained from the Indonesian saliva isolates had 96% (isolate 3.11) and 95% (isolate 5.14) values. This confirmed the presence of new strains based on average nucleotide identity (ANI). The isolate strains of 3.11 and 5.14 have been registered at DDBJ/EMBL/GenBank under the accession number LC382415 and LC382416, respectively. Conclusion: L. reuteri novel strain can be isolated from the saliva of Indonesian young adults.  Further studies involving biochemical tests and phenotypic analysis are needed to better understand these new L. reuteri strains.
Adjunctive Probiotics as Host Modulation Agent in Advanced Periodontitis: A Systematic Review of Randomized Controlled Trials Cherry Erlin Lindo; Boy Muchlis Bachtiar; Claudia Isabella; Karina Natalie Natalie; Karina Putri Juwita
Scientific Dental Journal VOLUME 8, ISSUE 1, JANUARY 2024
Publisher : Faculty of Dentistry, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/sdj.v8i1.19460

Abstract

Periodontitis, a complex oral inflammation, poses health risks, especially in advanced stages. Traditional treatments like nonsurgical periodontal therapy (NSPT) have limitations, prompting exploration of adjunctive approaches like probiotics. This systematic review focuses on randomized controlled trials (RCTs) assessing probiotics' impact when used alongside NSPT for advanced periodontitis. Periodontitis, a chronic oral condition, requires effective interventions, especially in advanced stages marked by severe tissue damage. Probiotics, particularly Lactobacillus and Bifidobacterium, have gained attention for their potential in supporting oral health. This review aims to analyse RCTs exploring probiotics as adjuncts to NSPT in advanced periodontitis. From 59 identified studies, 5 met inclusion criteria, published between 2019 and 2021. These studies examined probiotics' effects on probing pocket depth (PPD) and clinical attachment level (CAL) in stage III/IV Grade C periodontitis. Variations in probiotic strains, formulations, and therapy durations were observed. Risk of bias assessment indicated generally low risks across selected studies. Findings on PPD reduction were inconclusive, with two studies favouring probiotics, while others showed minimal differences. CAL gain was observed in all three studies assessing it, but no significant differences between probiotics and control groups were found. The benefits of adjunctive probiotics in advanced periodontitis remain unclear, contributing to the lack of firm conclusions. While probiotics show promise in influencing periodontal parameters, the current evidence does not firmly establish their efficacy as adjuncts to NSPT in advanced periodontitis. Homogeneous RCTs with standardized protocols are crucial for a more comprehensive evaluation of probiotics' potential benefits in this context
Co-Authors A. Fieki Rachmatillah Abas, Abdul Hawil Afifah Bambang Sutjiatmo, Afifah Bambang Aimee Monica Aimee Monica Alphianti, Likky T. Anissha D Putri Annisa Qashdina Alghassani Ariadna A Djais Armelia Sari Widyarman Basri A. Gani Basri A.Gani C Hanny Wijaya Celik, Ismail Cherry Erlin Lindo Claudia Isabella Dede R. Adawiyah Dewi Fatma Suniarti Diana S. Ningsih Diana Setya Ningsih, Diana Setya Elisabeth A Putri Endang W Bachtiar Endang W. Bachtiar Erwin Gunawan Euis Reni Yuslianti Fathia Agzarine Deandra Ghazi M. Idroes Ghazi Mauer Idroes Hedijanti Joenoes Heriandi Sutadi I. Wayan T. Wibawan Idroes, Ghazi M. Ikrima, Abidah Ira Tanti Karina Dhaniarti Karina Natalie Natalie Karina Putri Juwita Kemala, Pati Khairan Khairan Khairan Khairan Lisa Prihastari, Lisa Lukman Hakim Makmun Lukman Hakim Makmun Maryati Maryati Maulydia, Nur B. Maulydia, Nur Balqis Megantoro, Aryo Mindia Yuniastuti Mindya Yuniastuty Mindya Yuniastuty Mochamad Fahlevi Rizal Nasution, Aini Hariyani Natalina Natalina , Ningsih, Diana S. Nur B. Maulydia Nurtami Soedarsono Pati Kemala Paulina N. Gunawan Ratna Ramadhani Refyan, Sarah Athiyyahmaulidya Ria Puspitawati Rinaldi Idroes Rintoko, Bimo Robert Lesang Robert Lesang Robert Lessang Rohmah, Devi Kartika Santi Chismirina Sari, Bernadeta RE Silvia Sulistiani Stella Pranoto Sulistiani, Silvia Sutjiatmo, Afifah B Theodorea, Citra Fragrantia Tri Wibawa Trina E. Tallei Trina E. Tallei, Trina E. Wahyu Sulistiadi Widya Asmara Widyarman, Armelia Sari Yulianti Kemal Yulianti Kemal Yundari, Yundari Yuslianti, Euis Reni Zaki Mubarak Zinatul Hayati Zuchra Helwani