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ISOLASI DAN IDENTIFIKASI KHAMIR SECARA MORFOLOGI DI TANAH KEBUN WISATA PENDIDIKAN UNIVERSITAS NEGERI SEMARANG -, Jumiyati; Bintari, Siti Harnina; Mubarok, Ibnul
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 1 (2012): March 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i1.2265

Abstract

Tujuan penelitian untuk mengisolasi dan mengidentifikasi khamir secara morfologi di tanah Kebun Wisata Pendidikan Unnes. Penelitian ini merupakan penelitian eksplorasi yang dilakukan di Laboratorium Mikrobiologi Jurusan Biologi FMIPA Unnes. Populasi penelitian adalah khamir di tanah Kebun Wisata Pendidikan Unnes. Sampel penelitian adalah isolat khamir yang diambil dengan teknik Purposive Sampling yaitu membagi kebun menjadi lima zona dan setiap zona diambil secara acak lima titik pengambilan kemudian dihomogenkan. Sampel dibawa ke Laboratorium untuk dilakukan isolasi, purifikasi dan identifikasi secara morfologi koloni dan sel. Simpulan penelitian ditemukan tujuh isolat khamir dan termasuk ke dalam lima genus yaitu Saccharomyces, Candida, Debaromyces, Brettanomyces dan Saccharomycodes. The purpose of the research was to isolate and to identify morphologically the individually-isolated yeasts from the soil of Biology Educational Garden Semarang State University. The exploration research was conducted in the Microbiology Laboratory Department of Biology, FMIPA Semarang State University. The yeasts were collected using purposive sampling technique in five zones and from each zone five random plots were selected to obtain the samples. Samples were isolated, purified and identified morphologically in terms of the colonies and the cells. Result revealed that seven isolated yeasts from five generas (Saccharomyces, Candida, Debaromyces, Brettanomyces, and Saccharomycodes) had been collected from the soil of Biology Educational Garden Semarang State University.
Efek Interaksi Ragi Tape dan Ragi Roti terhadap Kadar Bioetanol Ketela Pohon (Manihot Utilissima, Pohl) Varietas Mukibat Kurniawan, Tri Budi; Bintari, Siti Harnina; Susanti, R.
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 2 (2014): September 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i2.3783

Abstract

Ketela pohon (Manihot utilissima, Pohl) varietas mukibat berpotensi sebagai bahan baku bioetanol. Penelitian bertujuan untuk mengetahui efek interaksi ragi tape dan ragi roti terhadap kadar bioetanol ketela pohon mukibat dan menentukan interaksi terendah yang efektif menghasilkan bioetanol yang dapat terbakar. Sampel yang digunakan adalah ketela pohon mukibat umur 7 bulan dengan berat rata-rata 500 gram. Penelitian menggunakan Rancangan Acak Lengkap pola faktorial, yaitu konsentrasi ragi tape (0%, 0,3%, 0,6% dan 0,9%) dan konsentrasi ragi roti (0%, 0,3%, 0,6% dan 0,9%) dengan 3 kali ulangan. Parameter yang diukur meliputi jumlah sel khamir (data pendukung) dan kadar bioetanol (data utama). Data kadar bioetanol yang diperoleh dianalisis menggunakan anava dua arah dan dilanjutkan uji Duncan (DMRT) pada taraf kesalahan 5%. Hasil penelitian menunjukkan ada pengaruh interaksi ragi tape dan ragi roti terhadap kadar bioetanol ketela pohon mukibat p>0,05. Konsentrasi ragi tape dan ragi roti yang tinggi menghasilkan kadar bioetanol yang tinggi. Interaksi ragi tape 0,6% dan ragi roti 0,3% (T2R1) merupakan interaksi terendah yang efektif menghasilkan kadar bioetanol yang dapat terbakar (36%). Interaksi ragi tape 0,9% dan ragi roti 0,9% (T3R3) menghasilkan kadar bioetanol tertinggi dengan rata-rata mencapai 49,8%.Cassava (Manihot utilissima, Pohl) var Mukibat is potential for bioethanol feedstock. The study aimed to determine the effects of the interaction of bread yeast and tape yeast on the concentration of mukibat cassava bioethanol and to determine the lowest effective interaction can produce flammable bioethanol. The samples used were mukibat cassava aged 7 months with an average weight of 500 grams. Research design was completely randomized design factorial, tape yeast concentration (0%, 0.3%, 0.6% and 0.9%) and the concentration of yeast bread (0%, 0.3%, 0.6% and 0.9%) with 3 replications. Parameters measured include the number of yeast cells (supporting data) and ethanol concentration (primary data). Bioethanol content data obtained were analyzed using two-way ANAVA and Duncan continued test (DMRT) at the level of 5% error. The results showed that no effect of the interaction of breads yeast and tape yeast on levels of cassava bioethanol p> 0.05. The concentration of bread yeast and tape yeast yielded in high concentration of ethanol. The interaction of yeast tape 0.6% and 0.3% bread yeast (T2R1) gave the lowest effective interaction produced 36% ethanol. The interaction of tape yeast 0.9% and 0.9% bread yeast (T3R3) resulted in the highest ethanol concentration with an average reached 49.8%.
Efek Inokulasi Bakteri Micrococcus luteus Terhadap Pertumbuhan Jamur Benang dan Kandungan Isoflavon pada Proses Pengolahan Tempe (Effect Inoculation of Micrococcus luteus to Growth of Mold and Content Isoflavone at Tempe Processing) Bintari, Siti Harnina; P, Anisa Dyah; J, Veronika Eka; R, Rivana Citra
Biosaintifika: Journal of Biology & Biology Education Vol 1, No 1 (2009): March 2009
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v1i1.43

Abstract

Tempe content of aglikon isoflavone which have potency to as anticancer compound. During processing of tempe, beside tempe mold (Rhizopus oligosporus) share other microbial namely bacterium. One of the bacterium residing at tempe ecosystem is Micrococcus luteus, having the character of gram positive and can conduct genistein biotransformation and daidzein become factor II compound (6,7,4-tri hydroksi isoflavone). Its problems how amount of R.oligosporus cell and M. luteus at processing of tempe associated is full scale of yielded tempe isoflavone. The study aimed to improve full scalely tempe aglikon isoflavone through inoculation of M.luteus bacteria. Used complete random designd, with M.luteus bacteria, as independent variable and variable depended is the amount of mold cell (R..oligosporus), amount of full scale M.luteus cell and total of isoflavone. Result of one way Anava indicate that inoculation of M.luteus bacteria do not degrade the amount of R.oligosporus cell with F call (53,28) > F tab (3,10) at significancy level 5%. Amount of M.luteus cell from 1,3 x 109 cell / gr [at] fermentation first becomes 3,4 x 106 cell/gr and decrease after day fermentation second becoming 1,75 x 106 cell/gr. Proportion of increasing isoflavone content at day fermentation 1-2 from 19,1 becoming 52,7 mg/100gr. Inoculation of M. luteus bacteria increased to count isoflavone aglicone and no trouble at growth of tempe mold and it relate to the increasing of is full scale of yielded tempe isoflavone.
Isolasi dan Identifikasi Virus Avian Influenza Subtipe H5N1 pada Unggas di Pasar Tradisional Semarang Ulum, Farikhul; Susanti, R.; Bintari, Siti Harnina
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 2 (2013): September 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i2.3914

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Meningkatnya kasus infeksi virus Avian Influenza (AI) subtipe H5N1 atau lebih dikenal dengan flu burung yang menyebabkan kematian pada manusia sangat dikhawatirkan dapat menular dari manusia ke manusia. Penelitian ini bertujuan untuk mendapatkan isolat virus Avian Influenza subtipe H5N1 pada unggas yang diperjualbelikan di pasar tradisional di Semarang. Sebanyak 55 sampel usap kloaka diambil dari unggas sehat dan belum divaksin di 6 pasar tradisional Kota Semarang. Inokulum ditumbuhkan pada telur ayam berembrio specific pathogen free (TAB-SPF) umur sembilan hari. Kemudian telur diinkubasikan selama 4 hari. Cairan alantois dipanen dan diuji kemampuannya mengaglutinasi sel darah merah. Cairan alantois yang menunjukkan aktivitas hemaglutinasi, selanjutnya diekstraksi RNA-nya dan diidentifikasi VAI subtipe H5N1 dengan metode Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) menggunakan primer spesifik H5 dan primer N1. Kemudian DNA hasil RT-PCR dianalisis dengan teknik elektroforesis. Hasil penelitian menunjukkan bahwa terdapat 4 isolat positif VAI subtipe H5N1 dengan sebaran 2 isolat dari sampel yang berasal dari pasar Mangkang, 1 isolat dari pasar Rejomulyo dan 1 isolat dari pasar Karimata. Berdasarkan hasil penelitian dan pembahasan dapat disimpulkan bahwa unggas yang diperjualbelikan di pasar tradisional di Kota Semarang ada yang terinfeksi VAI subtipe H5N1.The increasing cases of viral infection of Avian Influenza (AI) H5N1 subtype or more commonly known as bird flu that causes death in humans very feared to spread from human to human. The aim of this research was to obtain isolates of Avian Influenza virus (AIV) subtype H5N1 that marketable in traditional markets in Semarang. A total of 55 cloacal swab samples taken from healthy and unvaccinated fowl in the 6 traditional market in Semarang. Inoculum was grown in embryonated chicken eggs specific pathogen free (SPF TAB) nine days. Then the eggs were incubated for 4 days. Allantoic fluids were harvested and tested for their ability to agglutinate red blood cells. Allantoic fluid that showed hemagglutination activity, further their RNA was extracted and AIV subtype H5N1 identified with Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) method using specific primers primary H5 and N1. Then the results of RT - PCR were analyzed by electrophoresis technique. The results showed that there are 4 positive isolates with the distribution of the H5N1 subtype AIV 2 isolates samples derived from market Mangkang, 1 isolate from market Rejomulyo and 1 isolate from market Karimata. Based on the results of research and discussion, it can be concluded that the fowl that marketable in traditional markets in Semarang there were AIV infected with subtype H5N1.
Karakter Disiplin dan Percaya Diri Melalui Model Pembelajaran Problem Based Learning Materi Pertumbuhan dan Perkembangan Afifudin, Afifudin; Bintari, Siti Harnina; Ridlo, Saiful
Journal of Biology Education Vol 6 No 2 (2017)
Publisher : FMIPA UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbe.v6i2.19327

Abstract

Penelitian ini bertujuan untuk mendeskripsikan potret perkembangan dan pencapaian sikap disiplindan percaya diri siswa, serta faktor yang menyebabkan tidak terekspresinya sikap tersebut. Metodepengambilan data adalah observasi, dokumentasi, dan wawancara. Tujuan observasi adalahmemperoleh data skor siswa, sedangkan data faktor yang menyebabkan tidak terekspresinya karakterdisiplin dan percaya diri diperoleh melalui wawancara. Berdasarkan data skor menunjukkan potretsikap disiplin dan percaya diri siswa selama pembelajaran materi pertumbuhan dan perkembanganmelalui model PBL bersifat fluktuatif. Capaian sikap disiplin siswa secara klasikal tergolong baikdengan persentase 82,5%, sedangkan pencapaian sikap percaya diri tergolong cukup denganpersentase 65%. Selama proses pembelajaran seluruh siswa mengekspresikan karakter disiplin,namun pada karakter percaya diri terdapat 2 siswa yang tidak mengekspresikan. Berdasarkan datahasil wawancara, faktor utama yang menyebabkan siswa tidak mengekspresikan karakter percayadiri adalah perlakuan overdiscipline, overprotection, rejection, dan domination orang tua terhadap siswa.Faktor penyebab lainnya adalah sikap tidak efektif guru pada proses pembelajaran dan lingkungansosial (teman sebaya) siswa.   The study aims to describe the portrait of students in developing and achieving the discipline and confidence, andfactors that cause an unexpressed attitude. The method of data collection is observation, documentation, andinterview. The purpose of observation is acquiring the score data of the students, while the factors that lead to anunexpressed character of the discipline and confidence are obtained through the interview. Based on the scoredata, the students’ discipline and confidence during the material learning of growth and development throughPBL models are fluctuating. The achievement of the discipline attitude classically belongs to good with percentageof 82.5%, while the confidence attitude belongs to enough with percentage of 65%. During the learning process,all students expressed the character of discipline, but there were two students who did not express character of theconfidence. Based on the results of the interview, the main factor that caused the students unabled to express thecharacter of confidence was bacause of the over discipline treatment, overprotection, rejection, and parents’domination towards the students. Another factor is the ineffective attitude of teachers in the learning process andthe social environment (peer) students.
Correlation of Epistemic Curiosity and Cognitive Understanding Biology’s Student in Health and Pharmaceutical Biotechnology Istiani, Fahrun; Bintari, Siti Harnina; Widiatningrum, Talitha
Journal of Biology Education Vol 7 No 1 (2018): April 2018
Publisher : FMIPA UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbe.v7i1.22553

Abstract

Technology growing rapidly because technological development is required in all aspects of humanlife. Biotechnology is one of the branch of biology that is constantly evolving. Health andpharmaceutical biotechnology is one of the biotechnology which became the spotlight of the world.Biotechnology is part of science that commences from curiosity. One of curiosity type is epistemiccuriosity (EC). Epistemic curiosity contribute to give problem solving for the appearing issue. Thisneed higher order thinking. Higher order thinking need good cognitive understanding. The purpose ofthis study is to: 1) describe the epistemic curiosity (EC) Biology’ student to the development of healthand pharmaceutical biotechnology, 2) analyzing the correlation of epistemic curiosity (EC) andcognitive ability of biology’ student in health and pharmaceutical biotechnology. This researchincludes descriptive correlative with the research data is a cognitive understanding and EC ofbiology’ student. Cognitive understanding and EC data taken using a questionnaire (i-d scalecuriosity) and problem (the quiz). Average of EC scores is 26,1. A descriptive analysis of the averageEC is of 65.3% (high category). The average of students cognitive understanding is 38.8%. (lowcategory). Quantitative correlation of cognitive understanding and EC was 0,208 (weak category).Qualitative correlation of cognitive understanding and EC was J category, that have a high EC butlow cognitive understanding.
Implementation of the Practicum Methods with Guided-Discovery Model to the Student Skill of Science Process Hayati, Dwi Puji; Bintari, Siti Harnina; Sukaesih, Sri
Journal of Biology Education Vol 7 No 1 (2018): April 2018
Publisher : FMIPA UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbe.v7i1.23005

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Biology learning in SMA N 2 Batang still emphasized cognitive ability, has not yet oriented on the development of student skill of science process. The purpose of this research is to analyze theimplementation of practicum methods with guided-discovery model on students skill of science process. This research is Pre-Experimental with Pre-Test and Post Test Group Design. The sample was determined by purposive sampling, X MIPA 1 and X MIPA 2 as experiment class-1 and -2. The results obtained from the test and non-test. The result of skill of science process test was analyzed by N-gain. The average of N-gain test results from the experimental class is 11,4% in high category, 64,3% in medium category, and 24,3% in low category. Analysis of post-test result and student observation resulted in good category. The success of this method implementation in MIPA 1 is 95,15% and MIPA 2 is 88,9% with very high category. Students and teachers respond well to the implementation of practicum method with guided-discovery model. In conclusion the implementation of practicum method with guided-discovery model of environmental change material can develop student skill of science process in observing, classifyng, predicting, measuring, concluding, and communicating aspects.
Penerapan Model Pembelajaran Numbered Heads Together (NHT) terhadap Hasil Belajar Siswa pada Materi Sistem Ekskresi Yuliana, Lusia Retno; Susanti, R; Bintari, Siti Harnina
Journal of Biology Education Vol 7 No 2 (2018): August 2018
Publisher : FMIPA UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbe.v7i2.24265

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This aims of this research is to determine the application of NHT learning to student learning outcomes on the material of excretion system. The learning outcomes from this research is the result of learning in the affective and cognitive domain. Research with form of Pre-Experimental Design was held at SMPN 9 Semarang with class VIII D and VIII E as a research sample. Affective learning results show that 62,50% of students are in good categories and 29,69% are in very good category. The results of the N-gain test on students cognitive learning outcomes obtained from the posttest showed a gain of 0,61 with the medium improvement category. The final value analysis shows that 100% of students have complete their learning. Relatively, students give positive responses to the learning method that applied.  Learning is accomplished with a value 90% on very high implementation criteria. The conclusion of this research is the method of learning using NHT method can improve student learning outcomes in the material excretion system
The Potential of Microbial Symbionts Macrotermes gilvus Hagen Termite Gut as Degrading Agents of Cellulose in Bioethanol Production Susilowati, Dewi; Subekti, Niken; Bintari, Siti Harnina
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14965

Abstract

Water hyacinth is a potential feedstock for bioethanol production because of their high cellulose. The microbial symbionts of the Macrotermes gilvus termite’s gut have a high endoglucanase enzyme activity. This research was aimed to analyze the pH, temperature and agitation effects towards cell density, endoglucanase enzyme activity and reducing sugar, and to determine the effective optimum condition that can produce maximum reducing sugar. This research used central composite design (CCD) with the total number of run was . The independent variables were including pH (5.9, 6.4, 7.0, 7.6, 8.0), temperature (30 0C, 33 0C, 37 0C, 41 0C, 44 0C) and agitation (90 rpm,114 rpm, 150 rpm,185 rpm, 210 rpm), with six replications at central points. Parameters measured were cell density, endoglukanase enzyme activity and reducing sugar, thus analyzed by the statistical software package MINITAB 18.0. The Student’s t-test result showed the primary sequence influencing cell density as pH ˃ agitation ˃ temperature and towards endoglucanase enzyme activity and reducing sugar as pH ˃ temperature ˃ agitation, P ˂ 0.05. The maximum reducing sugar (60.13 ± 3.16 mmolL-1) was obtained at pH 6.95, temperature 37 0C and agitation 150 rpm. The results of this research can be used to explore the more potential microbial symbionts of the Macrotermes gilvus Hagen termite’s gut.
Scientific Attitudes, Activities and Learning Outcomes of Student in Material of Fungi Using Predict Observe Explain (Poe) Model Based on Bioedutainment Widyanigrum, Dewi; Bintari, Siti Harnina; Rahayuningsih, Margareta
Journal of Biology Education Vol 7 No 3 (2018): December 2018
Publisher : FMIPA UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbe.v7i3.25815

Abstract

This study aims to determine the effectiveness of POE model based on bioedutainmentimplementation in material of fungi towards activity, scientific attitude and learning outcome of VII grade students. This study uses a one-shot case study research design. The population of this study is students of class VII SMPN 1 Secang, while the sample is students of class VII B. The hypothesis used is that POE method based on bioedutainment is effective towards the learning outcome of the students.  The sample was taken by purposive sampling method. POE based on bioedutainment was applied to the sample class by using game elements in each POE syntax. The results of this study indicate the percentage of student activity is 83% and the percentage of their scientific attitude is 70% in the category of good and very good. The result of student learning completeness is 70% with an average value of 80.33. The teacher's response stated that students became active and enthusiastic about learning. The conclusion of the study is that the POE model based on bioedutainment is effective towards the activity, scientific attitude, and learning outcome of the students.
Co-Authors A.A. Ketut Agung Cahyawan W Alam, Aulia Nuanza Alamsyah - Alfiyan, Jamil Maulana Zahriyan Anam, M. Khairul Andin Vita Amalia, Andin Vita Anhari, Saeful Ani Nur Fauziah, Ani Nur Anik Ulfah, Anik Anisa Dyah P Anisa Dyah P Astrid Farmayati Caska - Chulia Mubtadiah, Chulia Danang Dwi Saputro Deddy Muchtadi DEDDY MUCHTADI Dewi Mustikaningtyas Dewi Susilowati Dhimas Fajar Eka Purnama Dwi Handayani DWI SUSILANINGSIH DWI YULIANTI Dyah Rini Indriyanti Eka - Mukaromah, Eka - Ely Rudyatmi, Ely Endah Peniati Enni Suwarsi Rahayu Evi Widowati Farikhul Ulum Farikhul Ulum Fazat Haniyya, Fazat Fernando, G. S.N. Fidia Fibriana Fitri Andiniyati Hadi Nasbey Hayati, Dwi Puji Hayati, Dwi Puji Heni Pujiastuti Ibnul - Mubarok, Ibnul - Ibnul Mubarok Ibnul Mubarok Ibnul Mubarok Ichsani, Nadya Iin Kurnia Iin Kurnia Intan Zainafree, Intan Isnaeni Karunia Annisa Istiani, Fahrun Istiani, Fahrun Jamil Maulana Zahriyan Alfiyan Jayanti, Septi Jumiyati - Jumiyati - Kartika Nugraheni Kartika Nugraheni Khairunisa, Nisrina L. Putri, Afra Fauziah Lail Isro, Abda Latipah, Rina April Lisdiana Lisdiana MADE ASTAWAN Mafaza Khaisuntaha Mafaza Khaisuntaha Maftuhah, Anis Margareta Rahayuningsih Nugroho Edi K Martien Herna Susanti Maryani Suwarno Maryani Suwarno Maryani Suwarno Masru'ah, Masru'ah Milah, Nihayatul Moeis, Siti Fatimah Muhammad Agus Muljanto Muhammad Khumaedi Mursyid . Mursyid Mursyid Mutiara Bintang Ramadani Nadya Ichsani Najah, Khoirun Nana Kariada Trimartuti Natalia Desy Putriningtyas, Natalia Desy Natalia, Lidya Ayu Nicky, Dhea Niken Subekti Nikmatul Hidayah Ning Setiati Ning Setiati Ning Setiati Nugraini, Anisa Ratna Nurika, Nurika P. Eko Prasetyo Parmin - Pramesti Dewi Pratama, Rizka Nur Priyantini Widiyaningrum Puput Roy Purnawati R. Susanti R. Susanti Rahina Nugrahani Rahmawati, Fahmi Retno - Istifarini, Retno - Retno Sri Iswari Rivana Citra R Rivana Citra r Rohman, Shohihatur Rosidah Rosidah Sadikin, Nadya Audina Nurkhafiya Saiful Ridlo Sarjadi Sarjadi Sarjadi Sarjadi Siti Alimah Siti Fatimah Moeis Siti Maesaroh Sri Sukaesih Sri Sukaesih Sri Utami Sri Widowati Sri Widowati Stephani Diah Pamelasari, Stephani Diah Sucihatiningsh Dian Wisika Prajanti, Sucihatiningsh Dian Wisika Sudarman Sudarman Sunyoto Sunyoto Sunyoto Sunyoto Syukron Rizqi, Syukron Talitha Widiatningrum Tiara Linanti, Annisa Tirtaningrum, Fitria Ayu Tri Budi Kurniawan Tri Budi Kurniawan, Tri Budi Tutik Wresdiyati Veronika Eka J Veronika Eka J Widya Hary Cahyati Widyanigrum, Dewi Widyanigrum, Dewi Winanti, Ruri Wiwi Isnaini Wulan Christijanti Wulandari K. E, Yenni Tyas Yanuar Revandi Yatin Mulyono Yuliana, Lusia Retno Yuliana, Lusia Retno Yuniarti Yuniarti Yustinus Ulung Anggraito