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All Journal Nexus Kedokteran Komunitas Nexus Kedokteran Klinik Nexus Biomedika Nexus Kedokteran Translasional Indonesian Journal of Medicine Oto Rhino Laryngologica Indonesiana Majalah Patologi Indonesia Abdimas Universal The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Plexus Medical Journal
Dyah Ratna Budiani
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Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Education Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences

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Hubungan antara Kejadian Abortus Spontan dengan Paritas dan Usia Ibu Hamil di RSUD Dr Moewardi Dwi Septiadi Badri; Eriana Melinawati; Dyah Ratna Budiani
Nexus Kedokteran Klinik Vol 3, No 2 (2014): Nexus Kedokteran Klinik
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Abortion is one of the health problems that causing high morbidity and mortality. there are various factors suspected to be associated with the cause of abortion including the factor of age and parity. A very young or a very old maternal age can increase the risk of abortion. So does the parity, the more parity of the mothers, the higher the risk of abortion. The purpose of this study was to determine the incidence of spontaneous abortion associated with maternal age and parity status during pregnancy. Methods: This study was an observational analytic study using cross-sectional. A total of 40 study subjects were selected by purposive random sampling was the pregnant women who become inpatients at the Department of Obstetrics and Gynecology at Dr. Moewardi State Hospital Surakarta who met the inclusion criteria . Data were collected by direct interview to the patient . The data were analyzed using logistic regression analysis test. Results: Based on the research status of pregnant women with parity ? 4 are 8.53 times likely to experience spontaneous abortion ( p = 0.000 , OR = 8.53 ). While pregnant women with age of < 20 years and > 35 years are 14.77 times likely to experience spontaneous abortion ( p = 0.000 , OR = 14.77 ). Conclusions: There is a significant relationship between the incidence of spontaneous abortion with parity and maternal age. Pregnant women with parity status ? 4 are 8.53 times likely to experience a spontaneous abortion while pregnant women with age < 20 years and > 35 years are 14.77 times likely to experience spontaneous abortion. Keywords: spontaneous abortion , parity , age
The Difference of Anxietys Score and Depressions Score between Mother with Mild Intellectual Disability and Mother with Moderate Intellectual Disability in SLB Negeri Surakarta Alindina Izzani; Aris Sudiyanto; Dyah Ratna Budiani
Nexus Kedokteran Klinik Vol 5, No 2 (2016): Nexus Kedokteran Klinik
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Children with Intellectual Disability (ID) have low Intelligence Quotient (IQ) and low social interaction so that they have difficulty in self-adaptation. Caring for child with disability will increase the risk of psychological morbidity in family such as anxiety and depression especially in mother. This study is aimed to discover the difference of anxietys score and depressions score between mother with mild intellectual disability and mother with moderate intellectual disability. Methods: This study was a cross-sectional observational analytic. 15 mothers of mild intellectual disability (C) and 15 mothers of moderate intellectual disability (C1) were recruited with consecutive sampling from SLB Negeri Surakarta on October 2015. Lie-Minnesota Multyphasic Personality Inventory (L-MMPI), Taylors Manifest Anxiety Scale (TMAS), and Becks Depression Inventory-II (BDI-II) questionnaires were used to assessed anxiety and depression. The test data was broken down and analyzed by independent t-test and the SPSS 20 for Windows software. Results: Mean and deviation TMAS score of mother with mild intellectual disability is 24,937,648 and mother with moderate intellectual disability is 23,009,736. There is no difference of anxietys score between two group of mothers (p = 0,55 ; p > 0,05). Mean and deviation BDI-II score of mother with mild intellectual disability is 11.279.08 and mother with moderate intellectual disability is 10.9310.39. There is no difference of depressions score between two group of mothers (p = 0,92; p > 0,05). Conclusions: There is no difference of anxietys score and depressions score between mother with mild intellectual disability and mother with moderate intellectual disability in SLB Negeri Surakarta. Keywords: Intellectual disability, Anxiety, Depression
Perbandingan Sensitivitas dan Spesifisitas antara Pengukuran Lingkar Lengan Atas, Lingkar Pinggang dan Lingkar Leher untuk Identifikasi Anak dengan Obesitas Cempaka Irawati; Hari Wahyu Nugroho; Dyah Ratna Budiani
Nexus Kedokteran Klinik Vol 3, No 2 (2014): Nexus Kedokteran Klinik
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Childhood obesity is a global phenomenon. Oneoften childreninthe worldare over weight. Then in Indonesia, the prevalence of childhood obesityis relatively increasedyear to year. Screening forobese childrenneeds to be doneearly, given thatobesity inchildhoodis likely to leadtoadultobesitylater. Anthropometric measurements are easy and inexpensive way. Therefore, there was some studies using anthropometryas the tool for identify obesityin addition to body mass index(BMI), which is the gold standard. However, there is no studies have compared the validity of assorted anthropometricas a tool to identify obese children, so that there searcherintends to compare the sensitivity and specificity between mid-upper arm circumference (MUAC), waist circumference (WC), and neck circumference (NC) in identification obese children. Methods: This study is observational analytic cross sectional approach. A sample included 72 children who were aged 6 to 12 years from four elementary school in Surakarta. Sampling population was obesity and healthy children (controls) who selected by simple random sampling had been carried out previously by fixed disease samples. Then the child was measured height, weight, MUAC, WC, and NC. Height and weight will be converted into BMI to determine obesity or not. Data were analyzed with diagnostic tests are grouped in table 2x2. From diagnostic test, we can determine the sensitivity and specificity. Furthermore, data were analyzed by Chi Square test and analysis receiver operating characteristic curve (ROC) to determine accuracy. Results: Among 72 children, 51,4% are male. MUAC (? percentile 90th), WC (? percentile 90th) and NC (cut off point 27,0-31,5) are significantly correlated with BMI. The sensitivity of MUAC was 58,33% with specificity 100%. The sensitivity of waist circumference was 66,67% with specificity 97,22%. The sensitivity of the neck circumference was 80,56% with specificity 63,89%. Conclusions: MUA Cisthe best measuring tool to identification obesityin children because it hasthe highest specificity, that is 100%. Keywords: Mid-upper arm circumference, waist circumference, neck circumference, obesity, children
Hubungan Antara Kejadian Abortus Spontan dengan Kadar Hemoglobin di RSUD Dr. Moewardi Andreas Peter Sitorus; Eriana Melinawati; Dyah Ratna Budiani
Nexus Kedokteran Klinik Vol 3, No 2 (2014): Nexus Kedokteran Klinik
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background : Many cases of abortions are caused by abnormal hemoglobin levels. World Health Organization (WHO) estimates that 35-75% of pregnant women in developing countries and 18% of pregnant women in developed countries undergo anemia while being pregnant. The purpose of this study is to determine the relationship between the incidence of spontaneous abortion with hemoglobin levels. Methods : This study is an analitic observational study with cross-sectional design. Its population are pregnant women who had been treated at the Obstetrics and Gynecology of Dr. Moewardi General Hospital. Subjects were taken with purposive random sampling technique. The data were collected using direct observation and subjects medical records. Data were analyzed using Chi Square. Results : The results of this study found 40 subjects due to the case of this study. Subjects with anemia who did not experience abortion counted only 2 people (5.0%) and subjects who experienced abortion counted as many as 17 people (42.5%). While subjects with normal hemoglobin levels who did not experienced abortion aggregates to 18 people (45%) and subjects who experienced abortion counted as many as 3 people (7.5%). The calculation obtained p-value = 0.000 <0.05, and OR = 0.020, which means there is a relationship between the incidence of spontaneous abortion with hemoglobin levels. Conclusions : There is a significant relationship between the incidence of spontaneous abortion with hemoglobin levels. Low hemoglobin levels will increase the risk of abortion.Keywords: abortion, hemoglobin levels, anemia
The Correlation between Inflammatory Cells Density and NF-kB Expression in Fibroinflammatory Nasal Polyps Krisnawati Intan Suwignyo; Dyah Ratna Budiani; Fikar Arsyad Hakim
Nexus Biomedika Vol 5, No 2 (2016): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Introduction: Asian nasal polyps have more neutrophil-dominant type than Europeans (>50%), the difference of histopathology feature may develope because different etiopathogenesis so they need to treat differently. NF-kB is a transcription factor that plays an important role in corticosteroid resistance mechanism in nasal polyps, while corticosteroid is the first choice of medical treatment for nasal polyps. The aim of this research was to determined the correlation between inflammatory cells density and NF-kB expression in fibroinflammatory nasal polyps. Methods: This analytic observational study had cross-sectional design which was done on 15 paraffin embedded fibroinflammatory nasal polyps tissues from patients who had their first nasal polyps surgery and fixed with buffered formalin. The blocks were taken from Anatomical Pathology Department of RSUD Dr. Moewardi and quota sampling was used to collect the samples. Each samples was stainned using hematoxylin-eosin and NF-kB immunohistochemistry technique, both inflammatory cells and NF-kB nuclear positive were counted, then analyzed using Spearman correlation. Result: Coeficient correlation (r) between lymphocytes and nuclear NF-?B in this study was 0.129 (p = 0.647), plasma cells and nuclear NF-?B was 0.404 (p = 0.135), macrophages and nuclear NF-?B was 0.367 (p = 0.178), while neutrophils and nuclear NF-?B was 0.285 (p = 0.301). Conclusions: There is no significant correlation between inflammatory cells density and NF-?B expression in fibroinflammatory nasal polyps. Key Words: nasal polyps, nuclear NF-kB, inflammatory cells
Pengaruh Ekstrak Air Buah Nanas terhadap Ekspresi Caspase-3 dan Fragmentasi DNA Sel Adenokarsinoma Kolorektal WiDr Sintin Khotijah Pribadi; Dyah Ratna Budiani; Riza Novierta Pesik
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Colorectal adenocarcinoma is the third most diagnosed neoplasm in the world. Many approaches have been taken for therapy, especially targeted therapy. However, the resistance event still occurs due to constitutive activation of intracellular signaling. In the other hand, pineapple fruit [Ananas comosus (L.) Merr.] has bromelain enzyme that can specifically influence intracellular signaling and not only lead to inhibit proliferation but also lead apoptosis induction in neoplasm cell. The purpose of this study was to elucidate the effect of pineapple fruit aqueous extract to caspase-3 expression and DNA fragmentation of apoptosis process in WiDr colorectal adenocarcinoma cells. Methods: This research was an experimental laboratory study with the post test only control group design. The samples of this research were WiDr colorectal adenocarcinoma cells divided into 6 groups: Negative control group, Positive control group with doxorubicin 5 ?g/ml and four Treatment groups with 270 ?g/ml, 202.5 ?g/ml, 135 ?g/ml and 67.5 ?g/ml of extract concentration, each. The samples were incubated for 48 hours in 37oC and 5% CO2. The caspase-3 expression was determined by cytologic score of samples stained with caspase-3 antihuman antibody. The DNA fragmentation was determined by 1% agarose gel electrophoresis. The cytologic score was analyzed by one way ANOVA test and LSD test. Result: The result showed that there was significant difference between Positive control group and the other groups (p=0.000), but there wasnt significant difference between the Negative control group and the Treatment groups (p>0.05). The result from electrophoresis showed that there wasnt DNA fragmentation in WiDr colorectal adenocarcinoma cells. Conclusion: The pineapple fruit aqueous extract did not increase caspase-3 expression and did not lead DNA fragmentation to WiDr colorectal adenocarcinoma cells for 48 hours of treatment. Keywords: pineapple fruit aqueous extract, WiDr colorectal adenocarcinoma cell, caspase-3 expression, DNA fragmentation
Metformin increases protein expression of Bax and p21 in WiDr cancer cell line Melani Ratih Mahanani; Dyah Ratna Budiani; Ari Natalia Probandari
Nexus Kedokteran Translasional Vol 4, No 1 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Colorectal cancer is malignancy in the gastrointestinal tract which leads to high morbidity and mortality rates globally. Colorectal cancer is the fourth highest cancer in Indonesia. 5-Fluorouracil (5-FU) is the first choice of chemotherapy for treating colorectal cancer but this therapy isless effective due to severeadverse effects. Metformin, a biguanidediabetes drug, has a potential anticancer, which is able to inhibit growth of some cancer cell lines like breast cancer, prostate cancer, hepatocellular cancer, and nasopharynx cancer. The aim of this study was to investigate protein expression ofBax (pro apoptotic protein) and p21 (cell cycle inhibitor) on colorectal cancer cell line treated with metformin. Methods: This study was a laboratory experimental study with the posttest only control group design. WiDrcancer cell line was cultured in RPMI1640 medium supplemented with 10% fetal bovine serum, 100 U penicillin and 100 g/ml streptomycin and incubatedin 5% CO2for 48 h at 37 C.Following day, cellswere treated with medium only, 7.68 mM5-FU and various doses of metformin(20, 10 and 5 mM) respectively. Protein expression of Bax and p21 was assessed by using immunocytochemistry staining against monoclonal antibodies antihuman Bax and p21.Intensity staining of each cell group was cytological scored and statistically analyzed by using Kruskal Wallis test. Results:Higher intensity score of Bax immunostaining was observed in WiDr cell line treated with three doses of metformin, compared to that of incontrol WiDr cell line and reached significantly difference with p= 0,00. Interestingly, these intensity scores of immunostaining were higher than the intensity score of immunostaining in WiDr cell line with 5-FU treatment. Moreover, administration of metformin increased intensity staining score against p21 monoclonal antibody with negatively dose dependent manner. WiDr cell line administered with 5 mM metformin had the highest intensity score of immunostaining but the intensity score was lower than the intensity score of immunostaining in WiDr cell line with 5-FU treatment. Conclusion: Metformin up regulates protein expression of Bax and p21 in WiDr cancer cell line and may become a promising anticancer. Further investigation is required to confirm the up regulation of both proteins. Keywords: Bax,colorectal cancer, metformin, p21
Hubungan antara Kadar Feritin dengan Kreatinin Serum pada Pasien Thalassemia di RSUD Dr. Moewardi Eko Dewi Ratna Utami; Harsono Salimo; Dyah Ratna Budiani
Nexus Kedokteran Klinik Vol 2, No 3 (2013): Nexus Kedokteran Klinik
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background : Patients with thalassemia that underwent routine blood transfusion are developing iron overload. In the state of excess iron, ferritin levels increase. When iron storage capacity has been depleted, free iron will cause tissue damage. One of that damaged organ is kidney. One of parameter used to know the function of kidney is GFR (Glomerular Filtration Rate). A simple way to estimate GFR is serum creatinine. Increased 1-2 mg/dL of creatinine serum showed decrease 50% of GFR. This research was aimed to determine the correlation between ferritin levels and serum creatinine of patients with thalassemia. Methods : A cross-sectional study which the subjects were 30 patients with thalassemia in the pediatric ward RSUD Dr. Moewardi who met the inclusion criteria. Ferritin levels and serum creatinine obtained from medical records or patient report book. Data normality was tested with Shapiro-Wilk normality test. Correlation between ferritin levels and serum creatinine were analyzed with non-parametric correlation test Spearman using Statistical Product and Service Solutions (SPSS) 20.0 for Windows. Results : Ferritin levels ranged from 1004.2 to 9600 ng/mL. Creatinine serum were 0.2 mg/dL; 0.3 mg/dL; 0.4 mg/dL; 0.5 mg/dL with almost equal distribution. Results of the analysis with non-parametric correlation test Spearman obtained R = 0.044 and p = 0.816. Conclusion : There was no correlation between ferritin levels and serum creatinine of patients with thalassemia. Keywords : thalassemia, ferritin, creatinine
Kombinasi Ekstrak Etanolik Kunyit dan Temulawak Lebih Berpotensi sebagai Fitofarmaka Hepatoprotektif: Studi Efek Hepatoprotektif pada Mencit Balb/C yang Diinduksi Parasetamol Atika Zahro Nirmala; S. Bambang Widjokongko; Dyah Ratna Budiani
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

The occurrence of liver damage especially caused by consuming non steroidal anti inflammatory medicine is high in some countries. In the other hand, turmeric and curcuma are frequently consumed as a herbal treatment. Those herbals contain antioxidant. The purposes of this experiment were to know the differences of hepatoprotective effect of ethanolic extract of turmeric, curcuma, and the combination of both in mice Balb/C induced by paracetamol. This research was a laboratory experimental study with the post test only control group design. The research used 25 male Balb/C mice that were devided into 5 groups: negative [KK (-)] and positive control group [KK (+)] were administered 0.2 ml of aquades, while group treatment 1 (KP1), 2 (KP2) and 3 (KP3) consecutively were administered 2.8 mg of turmeric ethanolic extract, 14 mg of curcuma ethanolic extract and the combination of 1.4 mg of turmeric extract and 7 mg of curcuma ectract. The treatments were conducted in 14 days. The dose of paracetamol toxic was treated to KK (+), KP1, KP2, and KP3 on the 12th, 13th, and 14th days. Hepatoprotective effect was assessed by the number of disfunctional cells of liver. The data were analyzed by One Way ANOVA and LSD test. The result of one way ANOVA showed a significant difference in the five groups with p = 0.03. LSD test showed a significant difference among KK (-), KP1, and KP3 against KK (+) and KP2. There were some differences of the hepatoprotective effect of ethanolic extract of turmeric, curcuma, and the combination of both in mice Balb/C induced by paracetamol. Keywords: hepatoprotective, turmeric, curcuma, paracetamol consecutively
Pengaruh kemoterapi neoadjuvant terhadap LMP1 dan rasio CD4+/CD8+ pada karsinoma nasofaring tak-berdiferensiasi Sunardo Budi Santoso; Muhardjo M; Made Setiamika; Imam Prabowo; Dyah Ratna Budiani
Oto Rhino Laryngologica Indonesiana Vol 42, No 2 (2012): Volume 42, No. 2 July - December 2012
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (459.568 KB) | DOI: 10.32637/orli.v42i2.30

Abstract

Background: Epstein-Barr virus (EBV) infection in undifferentiated type nasopharyngealcarcinoma (NPC) will express antigenic proteins such as LMP1 and triggering a cascade ofimmunocompetent cells (CD4+ and CD8+). The ratio of CD4+/CD8 illustrates the potential eliminationof intracellular pathogens and tumor cells. Neoadjuvant chemotherapy will suppress the growth of tumorcells and immune system cells   that leads to cellular immune decline. Objective: To know the influence ofneoadjuvant chemotherapy on the expression of LMP1, the immune system and the relationship betweenthe expression of LMP1 with the ratio of CD4+/CD8++. Method: The design was one group before andafter intervention, with 10 samples of undifferentiated NPC, biopsied before and after neoadjuvant chemotherapy, and got immunohistochemical examination. We used mouse antihuman LMP1, mouse monoclonal antihuman CD4+ and antihuman CD8 antibodies. Data were analyzed with the WilcoxonSigned Ranks test, and Spearman’s Linear Regression. Result: After neoadjuvant chemotherapy, we + found statistically significant decline in LMP1 expression (p = 0.007), CD4+ (p = 0.041) and CD8   (p= 0.005). The ratio of CD4+/CD8 increase was not statistically significant (p = 0.646). The relationshipbetween the expression of LMP1 with the ratio of CD4++/CD8was very weak (r = 0.17) and no statisticallysignificant (p = 0.646). Conclusion: Neoadjuvant chemotherapy in undifferentiated type NPC causes adecrease in the expression of LMP1 and immunological status (CD4 + +, CD8 ) and increase in the ratioof CD4+/CD8+. The relationship between the expression of LMP1 with the ratio of CD4+ was veryweak and not significant. Keywords: nasopharyngeal carcinoma, expression of LMP1, CD4+, CD8++/CD8, ratio of CD4,neoadjuvant chemotherapy.+++/CD8+  Abstrak :  Latar belakang: Infeksi Epstein-Barr virus (EBV) pada karsinoma nasofaring (KNF) jenis takberdiferensiasiakanmengekspresikan protein antigen antara lain LMP1 dan memicu hadirnyasel-selimunokompeten(CD4+ dan CD8+). Rasio CD4+/CD8 menggambarkan potensi eliminasi patogen intraseldan sel tumor. Kemoterapi neoadjuvant akan menghambat pertumbuhan sel tumor dan juga menghambatpembentukan sel-sel sistem imun tubuh sehingga berefek pada penurunan imunitas seluler. Tujuan:Mengetahui pengaruh kemoterapi neoadjuvant terhadap ekspresi LMP1, sistem imun dan hubunganantara ekspresi LMP1 dengan rasio CD4+/ CD8++. Metode: Desain penelitian one group before and afterintervention, menggunakan 10 sampel biopsi KNF tak-berdiferensiasi, sebelum dan sesudah kemoterapineoadjuvant dilakukan pemeriksaan imunohistokimia. Antibodi yang digunakan ialah antibodi mouseantihuman LMP1, monoclonal mouse antihuman CD4+ dan antihuman CD8. Data penelitian dianalisisdengan Wilcoxon Signed Ranks test, Regresi Linier dan Spearman’s dengan program statistik SPSS forWindows. Hasil: Setelah kemoterapi neoadjuvant terjadi penurunan signifikan secara statistik baikekspresi LMP1 (p=0,007); CD4+ (p=0,041), maupun CD8+ (p=0,005). Rasio CD4++/CD8 meningkat tidaksignifikan secara statistik (p=0,646).   Hubungan antara ekspresi LMP1 dengan rasio CD4 sangatlemah (r = 0,17) dan tidak signifikan secara statistik (p=0,646). Kesimpulan: Kemoterapi neoadjuvantpada KNF jenis tak-berdiferensiasi menyebabkan penurunan ekspresi LMP1 dan status imunologi(CD4+,CD8+) serta peningkatan rasio CD4+/CD8++++/CD8/CD8. Hubungan antara ekspresi LMP1 dengan rasio CD4/CD8 sangat lemah dan tidak signifikan. Kata kunci: karsinoma nasofaring, ekspresi LMP1, CD4++, CD8+, rasio CD4, kemoterapineoadjuvant.
Co-Authors Alindina Izzani Ambar Mudigdo Andreas Peter Sitorus Andreas Peter Sitorus Ari Natalia Probandari Aris Sudiyanto Arsita Eka Prasetyawati Atika Zahro Nirmala Betty Saptiwi Cempaka Irawati Cempaka Irawati Danus Hermawan Darmawan, Muhammad Dzaki Dhelima, Ismi Cahya Dwi Septiadi Badri Eko Dewi Ratna Utami Epistel Pangujian Simatupang Eriana Melinawati Farida Nurhayati Fikar Arsyad Hakim Hari Wahyu Nugroho Harsono Salimo Ida Ayu Sinthia Pradnya Swari Ida Nurwati Imam Prabowo Irving, Steven Jarot Subandono Jarot Subandono Krisnawati Intan Suwignyo Lutfi, Sephendra Made Setiamika Marcellus Simadibrata Marcellus Simadibrata Martini Martini Melani Ratih Mahanani Muhardjo M Muthmainah Muthmainah Nadzifah Nur Firdaus Ni Kadek Ayu Septiani Sarjana Novan Adi Setyawan Pertiwi, Ina Agustin Rezkita, Bastomy Eka Rifai Hartanto Rino Alvani Gani Rino Alvani Gani Risya Cilmiaty A. R. Riza Novierta Pesik Riza Novierta Pesik S. Bambang Widjokongko Sarsono Sarsono Selfi Handayani Setiawan, Novan Adi Sintin Khotijah Pribadi Sunardo Budi Santoso Zhahira, Thania Nur