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All Journal Nexus Kedokteran Komunitas Nexus Kedokteran Klinik Nexus Biomedika Nexus Kedokteran Translasional Indonesian Journal of Medicine Oto Rhino Laryngologica Indonesiana Majalah Patologi Indonesia Abdimas Universal The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Plexus Medical Journal
Dyah Ratna Budiani
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Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Education Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences

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Pengaruh kemoterapi neoadjuvant terhadap ekspresi NFκB dan c-myc pada karsinoma nasofaring jenis undifferentiated Farida Nurhayati; Muhardjo M; Made Setiamika; Dyah Ratna Budiani
Oto Rhino Laryngologica Indonesiana Vol 42, No 1 (2012): Volume 42, No. 1 January - June 2012
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.832 KB) | DOI: 10.32637/orli.v42i1.37

Abstract

Background: Epstein Barr, virus ( EBV ) infection has been frequently related with Nasopharyngeal Carcinoma (NPC), this virus makes the tissue of nasopharyng express Nuclear Factor Kappa Beta  (NFκB). NFκB and c-myc are two transcriptional factor for proliferation. NFκB and c-myc are two sign system for malignancy. The administration of neoadjuvant chemotherapy may prevent the apoptosis and inhibit the proliferation. Purpose: To evaluate the effect of neoadjuvant chemotherapy upon the expression level of NFκB and c-myc in undifferentiated NPC. Method: Quasi Experimental research by one group before and after intervention design. Ten sampels were collected from nasopharyngeal biopsy tissue which had been diagnosed as undifferentiated NPC. Wilcoxon signed Ranks test and Spearman’s were used to analized data, utilizing SPSS 15.0 underwindows program. Result: After neoadjuvant chemotherapy,   there were significant increase (p=0.005) for expression of NFκB (1.11 ± 1.14 / 4.92 ± 2.79) and significant  increase for expression of c-myc (1.15 ± 0,78 / 3.04 ± 1.91). There were significant corelation between  expresion of NFκB and c-myc in undifferentiated NPC (p=0.001). Conclussion: There were significant increase in expression of NFκB and c-myc after neoadjuvant chemotherapy in undifferentiated NPC, and   significant corelation between expresion of NFκB and c-myc in undifferentiated NPC. Keywords: nasopharyngeal carcinoma, neoadjuvant chemotherapy, NFκB and c-myc   Abstrak :  Latar belakang: Patogenesis Karsinoma Nasofaring (KNF) banyak dikaitkan dengan infeksi virus Epstein Barr. Virus ini menyebabkan jaringan mengekspresikan Nuclear Factor Kappa Beta (NFκB). NFκB  dan c-myc merupakan faktor transkripsi, yang menyebabkan proliferasi jaringan. Adanya ekspresi NFκB dan c-myc merupakan dua faktor penting sebagai penanda terjadinya keganasan. Pemberian kemoterapi neoadjuvant diharapkan dapat menghambat proses proliferasi dan menyebabkan terjadinya apoptosis. Tujuan: Mengetahui pengaruh kemoterapi neoadjuvant terhadap ekspresi NFκB dan c-myc pada KNF jenis undifferentiated dan hubungan antara ekspresi NFκB dan c-myc. Metode dan bahan penelitian: Penelitian eksperimen kuasi dengan rancangan one group before and after intervention.  Sebanyak 10 sampel dari jaringan biopsi KNF jenis undifferentiated, masing-masing dilakukan pemeriksaan ekspresi NFκB dan     c-myc sebelum dan sesudah pengobatan. Analisa dengan Uji statistk Wilcoxon signed Ranks test dan Spearman’s menggunakan SPSS 15.0 program underwindow. Hasil penelitian: Setelah kemoterapi neoadjuvant terjadi peningkatan signifikan (p=0,005) ekspresiNFκB (1,11 ± 1,14 dibanding 4,92 ± 2,79) dan terjadi peningkatan signifikan (p=0.025) ekspresi c–myc (1,15 ± 0,78 dibanding 3,04 ± 1,91). Analisis hubungan antara ekspresi NFκB dengan c-myc pada KNF jenis undifferentiated memenuhi garis liniar dan signifikan (p=0,001). Kesimpulan: Terdapat peningkatan ekspresiNFκB dan c-myc sesudah kemoterapi neoadjuvant yang signifikanpada KNF jenis undifferentiated. Terdapat hubungan signifikan antara ekspresi NFκB dengan c-myc pada KNF jenis undifferentiated. Kata kunci: karsinoma nasofaring, kemoterapi neoadjuvant, NFκB dan c-myc
The Difference Expressions of EBNA-1 in Epstein-Barr Virus Infection in Low and High Grade Colorectal Carcinoma Epistel Pangujian Simatupang; Marcellus Simadibrata; Rino Alvani Gani; Dyah Ratna Budiani
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 13, NUMBER 1, April 2012
Publisher : The Indonesian Society for Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (773.777 KB) | DOI: 10.24871/13120122-7

Abstract

Background: Colorectal carcinoma is a common malignancy with the highest mortality rate. Epstein- Barr virus (EBV) as the virus that most commonly infect humans, also can infect the body in a latent and induce the occurrence of malignancy. This study aimed to prove an association between EBV virus infection with degree of colorectal carcinoma by examining the main EBV oncogene expression, namely Epstein-Barr nuclear antigen-1 (EBNA-1), in low grade and high grade colorectal carcinoma. Method: Cross-sectional study was performed in 14 colorectal cancer patients in Moewardi Hospital, Surakarta between July 2011 and January 2012. The biopsy specimens were stained for EBNA-1 expression using immunohistochemical technique. Statistical analysis was performed using T-test and Mann-Whitney by SPSS software version 19.0 for windows. Results: Of the 14 patients, there were 7 patients with low grade colorectal carcinoma and 7 patients with high grade colorectal carcinoma. EBNA-1 expression was found in epithelium of low grade and high grade colorectal carcinoma with p = 0.01; CI = -5.24-0.88. We also assessed the expression of EBNA-1 on lymphocytes B of low grade colorectal carcinoma and high grade colorectal carcinoma with p = 0.043. Conclusion: Significant differences in the expression of EBNA-1 was found in association with EBV infection either in low grade and high grade colorectal carcinoma. The role of EBNA-1 as tumor initiator needs to be elucidated further. Keywords: carcinoma colorectal, Epstein-Barr virus, EBNA-1
A Ekstrak Etanolik Daun Kelor (Moringa oleifera, Lam) Menurunkan Ekspresi Caspase-3 Testis Tikus Wistar (Rattus norvegicus) Model Sindrom Metabolik Terinduksi Zhahira, Thania Nur; Budiani, Dyah Ratna; Setyawan, Novan Adi
Plexus Medical Journal Vol. 3 No. 2 (2024): April
Publisher : Fakultas Kedokteran, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/plexus.v3i2.229

Abstract

Pendahuluan: Sindrom metabolik berpengaruh terhadap kesuburan pria karena dapat menyebabkan infertilitas. Infertilitas antara lain disebabkan oleh kematian berlebih sel – sel spermatogenik selama spermatogenesis. Caspase-3 memiliki peran penting sebagai eksekutor apoptosis. Kandungan ekstrak etanolik daun kelor diketahui dapat mencegah apoptosis dengan menurunkan ekspresi caspase-3. Penelitian ini dilakukan dengan tujuan mengetahui pengaruh peningkatan dosis ekstrak etanolik daun kelor terhadap tingkat ekspresi caspase-3 pada jaringan testis tikus Wistar model sindrom metabolik. Metode: Metode penelitian yang digunakan adalah eksperimental laboratorik. Tikus Wistar berjumlah 30 ekor dibagi menjadi 5 kelompok dengan jumlah yang sama. KI sebagai kelompok kontrol negatif, KII sebagai kelompok sindrom metabolik terinduksi, KIII, KIV, dan KV sebagai kelompok sindrom metabolik terinduksi yang diberi dosis ekstrak etanolik daun kelor secara berurutan sebesar 150 mg/kgBB, 250 mg/kgBB, dan 350 mg/kgBB. Ekspresi caspase-3 dihitung menggunakan metode semikuantitatif IDS. Selanjutnya, ekspresi caspase-3 dianalisis menggunakan uji One-Way ANOVA, Post Hoc Tukey HSD, dan uji regresi linier. Hasil: KII memiliki skor ekspresi caspase-3 tertinggi yaitu 89,45 dan KV memiliki skor ekspresi caspase-3 terendah yaitu 25,93. Hasil uji One-Way ANOVA ekspresi caspase-3 pada jaringan testis tikus Wistar adalah 0.00 (p<0.05) yang artinya terdapat perbedaan signifikan ekspresi caspase-3 pada semua kelompok. Perbedaan bermakna terdapat antara KI dengan KII dan KII dengan KIII, KIV, dan KV. Hasil uji regresi linier menunjukkan pengaruh negatif antara dosis ekstrak etanolik daun kelor terhadap ekspresi caspase-3. Kesimpulan: Pemberian dosis ekstrak etanolik daun kelor yaitu 150 mg/kgBB, 250 mg/kgBB, dan 350 mg/kgBB dapat menurunkan ekspresi caspase-3.
Pengaruh Ekstrak Etanolik Lidah Buaya (Aloe vera) Terhadap Tingkat Maturasi Sel Sperma pada Testis Tikus Wistar (Rattus norvegicus) Model Sindrom Metabolik Terinduksi Nadzifah Nur Firdaus; Setiawan, Novan Adi; Budiani, Dyah Ratna; Riza Novierta Pesik
Plexus Medical Journal Vol. 3 No. 5 (2024): Oktober
Publisher : Fakultas Kedokteran, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jhfbgj54

Abstract

Pendahuluan: Sindrom metabolik memunculkan gangguan spermatogenesis, akibat adanya peningkatan ROS. Peningkatan ROS mengganggu maturasi sel sperma. Ekstrak etanolik daun lidah buaya memiliki potensi sebagai antioksidan. Tujuan penelitian ini mengetahui pengaruh ekstrak etanolik daun lidah buaya (Aloe vera) terhadap tingkat maturasi sel sperma menggunakan Johnson score. Metode: Penelitian eksperimental laboratorik dengan pretest-posttest control group design. Dua puluh lima tikus Wistar jantan dibagi menjadi 5 kelompok, masing-masing kelompok sebanyak 5 tikus. K1-kelompok kontrol, K2-kelompok sindrom metabolik, K3, K4, dan K5 adalah kelompok tikus dengan sindrom metabolik yang diberi ekstrak etanolik daun lidah buaya dosis 200, 250, dan 300 mg/KgBB/hari. Penghitungan Johnson score menggunakan perparat histopatologi dengan pengecatan Hematoxilin Eosin (HE) setelah terminasi pada hari ke-57. Analisis data menggunakan uji one-way ANOVA, dilanjutkan dengan uji post-hoc Tukey HSD, dan uji korelasi Pearson, untuk mengetahui hubungan antara dosis ekstrak etanolik Aloe vera dengan tingkat maturasi sperma. Hasil:. Berdasarkan hasil uji korelasi Pearson’s kenaikan Johnson score (tingkat maturasi sel sperma) sejalan dengan peningkatan dosis ekstrak etanolik daun lidah buaya dengan R2= 0,783. Hasil uji beda one-way ANOVA terdapat perbedaan antar kelompok p<0,05. Uji post-hoc Tukey HSD K1 secara signifikan berbeda dengan K2. K1 tidak berbeda dengan kelompok K3, K4 dan K5. K2 secara signifikan berbeda dengan K3,K4 dan K5. Rata-rata skor Johnson K1 9,92 0,05, K2 7,04±1,49, K3 8,93 0,76, K4 9,50 0,23, dan K5 9,56 0,53. Kesimpulan: Pemberian ekstrak etanolik daun lidah buaya (Aloe vera) dosis 200 mg/KgBB/hari, 250 mg/KgBB/hari dan 300 mg/KgBB/hari berpengaruh positif terhadap maturasi sperma tikus Wistar model sindrom metabolik.
Effect of Ethanolic Extract in Moringa oleifera, Lam. Leaf on Uric Acid Levels and Body Surface Area of Kidney Glomerulus in Wistar Rats (Rattus novergicus) Induced Metabolic Syndrome Model Darmawan, Muhammad Dzaki; Subandono, Jarot; Setyawan, Novan Adi; Budiani, Dyah Ratna
Indonesian Journal of Medicine Vol. 6 No. 4 (2021)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (32.019 KB)

Abstract

Background: There has been an increasing prevalence of metabolic syndrome (MS) caused by life style such as sedentary behavior and western diet. Metabolic syndrome causes degeneration in ren’s structure and processes the elimination of by product in metabolism, which is uric acid. Moringa oleifera Lam. leaves contain antioxidant that can repair damages caused by MS. Studies about improvement of ren’s structure and uric acid level related to Moringa oleifera Lam. leaves’ consumption has not been found yet. Therefore, this study was intended to examine the effects of ethanolic extract in Moringa oleifera Lam. leaves to uric acid and glomerular surface area in male wistar rat (Rattus norvegicus) with induced metabolic syndrome model.Method: This study is an experimental laboratory study. The subjects of this study consisted of 30 rats which were divided into 5 groups with 6 in each group. K1 is control group, K2 is MS group, and K3, K4, and K5 are MS groups given variety of ethanolic extract doses. The induction of MS was done by giving high-fat diet in 28 days and injection of streptozotocin-nicotinamide (STZ-NA) in the 25th day. Rats in group K3, K4, and K5 were given doses of 150, 250, and 350 mg/kgBW in 28 days.Results: The administration of high-fat diet for 28 days and injection of STZ-NA caused MS condition in rats.  Repeated ANOVA and One-Way Anova test showed that the administration of ethanolic extract in Moringa oleifera Lam. leaves with doses of 150, 250, and 350 mg/kgBW in 28 days decreased uric acid significantly (p= 0.001; p=0.001; p= 0.001). Another result also found that ethanolic extract from Moringa oleifera Lam. leaves with doses of 250 and 350 mg/kgBW increased area of glomerular surface area in rats significantly.Conclusion: The administration of ethanolic extract from Moringa oleifera Lam. leaves with doses of 150, 250, and 350 mg/kgBW for 28 days decreased uric acid level in rats. Ethanolic extract of Moringa oleifera Lam. leaves 250 and 350 mg/kgBw doses increase glomerular surface area.Keywords: Moringa Oleifera, uric acid, glomerular cross-sectional area, metabolic syndrome, kidneyCorrespondence: Muhammad Dzaki Darmawan. Study Program of Medical Doctor Education, Faculty of Medicine, Universitas Sebelas Maret. Email: mdddzaki@student.uns.ac.id.Indonesian Journal of Medicine (2021), 06(04): 412-422https://doi.org/10.26911/theijmed.2021.06.04.07
Effect of Ethanolic Extract of Moringa oleifera, Lam. Roots on Expression of Cyclin D1 in Testicular Tissue of Metabolic Syndrome Induced Wistar Rats Lutfi, Sephendra; Budiani, Dyah Ratna; Setyawan, Novan Adi
Indonesian Journal of Medicine Vol. 8 No. 2 (2023)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26911/theijmed.2023.08.02.05

Abstract

Background: Metabolic syndrome (MS) impacts on inhibition process of spermatogenesis by damaging various components of cell cycle proteins. Cyclin D1 protein which plays role in regulating the cell cycle of spermatogenic cells is depleted due to the metabolic syndrome. Variation results of M. oleifera studies on fertility are interesting for further research. This study aimed to determine the effect of ethanolic extract of M. oleifera roots towards expression of testicular cyclin D1 of metabolic syndrome induced Wistar rats.Subjects and Method: This study was an experimental laboratory. Thirty subjects of male Wistar rats were randomly divided into 5 groups consisted of 6 rats, control group (K1), MS group (K2), and treatment groups (K3, K4, and K5) which were given doses of 150, 250, and 350 mg/kgBW, respectively for 28 days. Induction of MS was achieved by high-fat diet for 28 days and injection of streptozotocin-nicotinamide (STZ-NA) on day 25. Cyclin D1 data was calculated using Intensity Distribution Score (IDS) and analyzed using one-way ANOVA test followed by post hoc multiple comparisons with Tukey HSD. Simple liniar regression test conducted on cyclin D1 data of K2, K3, K4, and K5.Results: One-way ANOVA test showed that the administration of ethanolic extract M. oleifera Lam. roots with doses of 150, 250, and 350 mg/kgBW in 28 days increased cyclin D1 significantly (p= 0.000). There were significant differences between K1 and K2 (p= 0.000), K2 and K3 (p= 0.000), K2 and K4 (p= 0.000), K2 and K5 (P= 0.000). Liniar regression test showed significant (p= 0.000) and moderate effect (R2= 0.65) of doses of ethanolic extract of M. oleifera roots on the expression of cyclin D1.Conclusion: The administration of ethanolic extract Moringa oleifera Lam. roots with doses of 150, 250, and 350 mg/kgBW for 28 days increased cyclin D1 in testicular rats.
Hepatoprotector Effect of Moringa Oleifera Lam. Seeds Extract Through Decrease Liver Inflammation of Rats with Metabolic Syndrome Rezkita, Bastomy Eka; Pertiwi, Ina Agustin; Dhelima, Ismi Cahya; Budiani, Dyah Ratna; Irving, Steven
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy Vol 23, No 3 (2022): VOLUME 23, NUMBER 3, December 2022
Publisher : The Indonesian Society for Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24871/2332022174-179

Abstract

Background: Metabolic syndrome has been associated with chronic inflammation due to the increase of lymphocyte focus on hepatic lobular tissue. Moringa oleifera Lam. is an herbal plant that potentially reduce the inflammation process. This study aims to evaluate the effect of Moringa seed extract (MSE) on hepatic lobularinflammation in rats with hepatic tissue metabolic syndrome (MetS).Method: Twenty-four male Wistar rats (n = 24) were assigned into four groups: one control group (C) and three MetS groups, fed with a high-fat, high-fructose diet (HFHFD) daily for 53 days. After 53 days, respectively, MetS 2 and MetS 3 groups were given 150 and 200 mg/kg MSE. After 28 days of MSE administration, the rats were sacrificed, and then hepatic lobular inflammation measured with lobular inflammation score. The effect of MSE on hepatic lobular inflammation was analyzed with Kruskal-Wallis and Mann-Whitney test.Results: There was a significant difference in hepatic lobular inflammation between four groups (p = 0.000). Mann-Whitney showed a significant difference between C vs. MetS 1, MetS 1 vs. MetS 2, and MetS 1 vs. MetS 3 (p = 0.000), but not significant C vs. MetS 2 (p = 0.364), C vs. MetS 3 (p = 0.109), MetS 2 vs. MetS 3 (p = 0.533).Conclusion: MSE at the dose of 150 mg/kg and 200 mg/kg significantly reduces lobular inflammation in hepatic tissue of MetS rats.
Co-Authors Alindina Izzani Ambar Mudigdo Andreas Peter Sitorus Andreas Peter Sitorus Ari Natalia Probandari Aris Sudiyanto Arsita Eka Prasetyawati Atika Zahro Nirmala Betty Saptiwi Cempaka Irawati Cempaka Irawati Danus Hermawan Darmawan, Muhammad Dzaki Dhelima, Ismi Cahya Dwi Septiadi Badri Eko Dewi Ratna Utami Epistel Pangujian Simatupang Eriana Melinawati Farida Nurhayati Fikar Arsyad Hakim Hari Wahyu Nugroho Harsono Salimo Ida Ayu Sinthia Pradnya Swari Ida Nurwati Imam Prabowo Irving, Steven Jarot Subandono Jarot Subandono Krisnawati Intan Suwignyo Lutfi, Sephendra Made Setiamika Marcellus Simadibrata Marcellus Simadibrata Martini Martini Melani Ratih Mahanani Muhardjo M Muthmainah Muthmainah Nadzifah Nur Firdaus Ni Kadek Ayu Septiani Sarjana Novan Adi Setyawan Pertiwi, Ina Agustin Rezkita, Bastomy Eka Rifai Hartanto Rino Alvani Gani Rino Alvani Gani Risya Cilmiaty A. R. Riza Novierta Pesik Riza Novierta Pesik S. Bambang Widjokongko Sarsono Sarsono Selfi Handayani Setiawan, Novan Adi Sintin Khotijah Pribadi Sunardo Budi Santoso Zhahira, Thania Nur