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All Journal Agrikultura Zuriat Jurnal Fakultas Pertanian Buletin Plasma Nutfah JURNAL AGROTEKNOLOGI Planta Tropika Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Kultivasi Jurnal Sains Teh dan Kina Chimica et Natura Acta Syntax Literate: Jurnal Ilmiah Indonesia Jurnal Riset Akuakultur AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Teknika Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Jurnal Indonesia Sosial Teknologi Perwira Journal of Economics and Business (PJEB) Perwira Journal of Community Development (PJCD) TarbiyahMU
Nono Carsono
Fakultas Pertanian Universitas Padjadjaran
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Journal : JURNAL AGROTEKNOLOGI

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DETEKSI GEN KETAHANAN HAWAR DAUN BAKTERI PADA PADI FAMILI F7 PERSILANGAN SINTANUR X PTB33 DAN FAMILI F5 PANDANWANGI X PTB33 SEBAGAI UPAYA AWAL SKRINING GENOTIPE POTENSIAL Prayitno, Prayitno; Carsono, Nono; Anas, Anas; Mahardhika, Dimas Kembara
Jurnal Agroteknologi Vol 15, No 2 (2025): Februari 2025
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v15i2.35238

Abstract

The decrease in rice production due to bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) has been reported to be quite high, ranging from 15–23%. Based on its spread and rice losses due to this pathogen, efforts are highly needed to reduce and control it. The BLB resistance gene is called the Xa gene. Currently, there are 42 Xa genes controlling BLB resistance that have been identified in some cultivars, mutant population and wild species of rice. The Xa3, Xa4 and xa8 genes are the three well-known of bacterial leaf blight resistance genes in hybrid rice in China and South Asian countries. Considering their dominant existence in Indonesia, molecular screening by means of PCR detection has been done in eight families of F7 Sintanur x PTB33 and two families of F5 Pandanwangi x PTB33 for these three Xa genes. Molecular markers BB3RF, BB3RR, MP1, MP2, and RM21044 were applied to detect those three genes. Based on DNA visualization it was found that SP101-3-1-5-8, SP101-3-1-19-26, SP101-3-1-38-4, SP87-1-1-7-7, PP48-5-1, and PP48-5-24 had the Xa3 gene. The genotypes that carry the Xa4 gene were SP101-3-1-19-27, SP101-3-1-38-4 and SP101-3-1-38-25. Meanwhile, the xa8 gene was only detected in Pandanwangi, meanwhile the SP101-3-1-38-4 carried the Xa3 and Xa4 genes. No genotypes were found with three genes. The selected genotypes will be further developed in order to assembly multiple resistance genes for this particular disease.
Co-Authors Abd. Rasyid Syamsuri Ade Ismail Adilah Nurul Fitrah Afifah, Zulfa Agung Karuniawan Al Findy Yuhibba Fitriah Amalia Purdianty Amaliah, Lia Anas Anas Anas Anas, Anas Anas Zubair, Anas Anggita Dewi Ani Apriani Anita R. S. Wardani Anita Rachmasari S.W. Arif Solehudin Baehaki S.E. Baehaki S.E. Bambang Sriyadi Bambang Sriyadi, Bambang Betty Natalie Fitriatin Christian Bachem Christian Bachem, Christian Citra Bakti, Citra Danar Dono Dedi Ruswandi Desy Lidiasari Dewi, Atri Prautama Dwi Ariyani Eldikara, Ranggi Ema Komalasari Ema Komalasari, Ema Fachrunnisa, Laras Sitta Fadli Kurnia Farida Damayanti Feliatra Fitri Utami Hasan Fitri Widiantini Gatut Bayuaji Gigih Ibnu Prayoga Hasan, Fitri Utami Hasan, Fitri Utami Hera Hera Heri Syahrian Heri Syahrian, Heri Hersanti - Ibnu Dwi Buwono Ibrahim, Muhammad Yasser Inez Nur Indrayani Irma Mangatur Juniati, Atie Tri Kang, Seung Won kinya toriyama Leni Nurlaeli Irmayanti Lukman, Pradita N. M. Untung Kurnia Mahardhika, Dimas Kembara Mas Dadang Enjat Munajat Maulana, Haris Meddy Rachmadi Mitalo, Oscar W. Muhamad Kadapi Murdaningsih H. Karmana Nenet Susniahti Neni Rostini Neni Rostini Neni Rostini Nita Fitria Noladhi Wicaksana Nuryani Tinumbia, Nuryani Pradita N. Lukman Prayitno Prayitno Puji Syara Anggia Putra, Fitriansyah Rangga Jiwa Wibawa Ranggi Eldikara Reginawanti Hindersah Reisyi Rinola Tambunan Riski Gusri Utami Riski Gusri Utami Riski Gusri Utami Rofiuddin, Muhammad M. A. S Imam Wahyudi Safitri, Adam Santika Sari Santika Sari Santika Sari Santika Sari Santika Sari Santika Sari Santika Sari Santika Sari Santika Sari Santika Sari Sari, Santika Sari, Santika SATRIYAS ILYAS Slamet, Whitea Yasmine Soedarsono Soedarsono Solehudin, Arif Sri Nurlianti Syindy Raffini Nasihin Tomohiko Yoshida Tomohiko Yoshida, Tomohiko Tri Joko Santoso Untung Susanto Untung Susanto Veithzal Rivai Zainal Vitria Puspitasari Rahadi Vitria Puspitasari Rahadi Wendy Wijaya Whitea Yasmine Slamet Wieny H. Rizky Yoana Saragih Yuniar Mulyani Zubair, Anas Zulfa Afifah