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All Journal ACTA VETERINARIA INDONESIANA Jurnal Manajemen dan Agribisnis Hemera Zoa Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Jurnal Kesehatan Lingkungan indonesia BERITA BIOLOGI Jurnal Medik Veteriner JURNAL KAJIAN VETERINER Livestock and Animal Research Jurnal Kedokteran Hewan Current Biomedicine Journal of Livestock and Animal Health (JLAH)
Herwin Pisestyani
Department Of Veterinary Public Health, Department Of Animal Disease And Veterinary Public Health, Faculty Of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Journal : Jurnal Sain Veteriner

 1 
Microbiological Quality of Milk Flavored Ready to Drink and Hygiene Practice of Street Vendors Herwin Pisestyani; Irena Ivania; Ardilasunu Wicaksono; Denny Widaya Lukman; Mirnawati Sudarwanto
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.60996

Abstract

Milk products consumption has become a new trend of healthy lifestyle among students in order to meet appropriate daily nutrients. This study was aimed to measure the total number of microbes found in milk drinks sold around the IPB Dramaga and Cilibende and to determine factors that influence it. Samples were collected from 13 milk vendors within radius of 2 kilometers from outermost point of campus and taken with 3 repetitions. Calculation of the total number of microbes was done by using the plate count method in accordance with SNI 2897-2008 about testing method of microbial contamination in meat, eggs, and milk, as well as processed products and SNI 01-6366-2000 about the maximum limit of microbial contamination in food. Risk factors’ data was taken by interviewing milk drinks seller using structured questionnaire. Factors that influenced microbial contamination were analyzed using Chi-Square test. The results showed that 74.4% of milk drinks samples had a high total number of microbes. The average total number of microbes in Dramaga and Cilibende samples was 1.0 x 105 ± 1.4 x 105 cfu / ml and 1.1 x 107 ± 1.2 x 107 cfu/ml. The total number of microbes are significantly influenced (p<0.05) by equipments used, the cleanliness of the equipment, the cleanliness of the table, washing hands practice, and the distance from the crowd. The high number of microbial contamination in milk drinks could damage the quality of milk and shorten the shelf life.
Slaughterhouse’s Animal Welfare Assessement at Bekasi City, West Java Katyusa Nandini Dyah Pitaloka; Etih Sudarnika; Herwin Pisestyani
Jurnal Sain Veteriner Vol 41, No 3 (2023): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.84696

Abstract

The quality of meat sold in Bekasi City is affected by the implementation of animal welfare during the slaughtering process in Slaughterhouses. The research aimed to analyze the animal welfare implementation during the slaughtering process at Bekasi City slaughterhouses. The data was conducted by observing the five-freedom implementation for five slaughterhouses throughout Bekasi City, from the shelter cages to the slaughter. Herding officers ensured no disturbance in the herding route, hitting only animals' backs when the animals stopped walking, and confirmed that animals did not pile up on the herding lane and that live animals did not see the slaughter process. However, some officers still wear clothing that contrasts with the environment, hit the animals repeatedly, and shout at the animals excessively during the herding process. Animal welfare aspects of the slaughter process, such as officers already in position when the animal enters the restraining box, the sharpness of the knife being checked before slaughtering, having a spare knife, and animals slaughtered less than 30 seconds after stunning, have been implemented by all officers. The butcher animal welfare implementation, such as checking the animals' death after being slaughtered, still needs to be improved. This research concluded that Bekasi City's slaughterhouse officers had implemented aspects of animal welfare. However, some improvements are still required through training to increase the competence of officers.
Escherichia coli O157:H7 Bacteria Antibiotic Resistant Isolated from Flies at Food Courts in IPB Dramaga Campus Aminudin, Muhammad Rizki; Lukman, Denny Widaya; Sudarwanto, Mirnawati Bachrum; Pisestyani, Herwin
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.99843

Abstract

Several human and animal pathogens transmit into the food chain through houseflies as mechanical vectors, one of which is E. coli O157:H7. E. coli O157:H7 can express Shiga toxin (Stx) which can cause diarrhea, hemorrhagic colitis, and potentially fatal hemolytic-uremic syndrome (HUS) in humans. Some pathogen strains show resistance against various antibiotics, causing complex health problems. This study aims to analyze the presence of antibiotic-resistant E. coli O157:H7 bacteria carried by houseflies (M. domestica) in the food court IPB Dramaga campus area. Detection of E. coli O157:H7 on fly legs using qPCR method based on MU 7.2.3.32-8. E. coli O157:H7 isolates were tested for sensitivity to the antibiotic’s ciprofloxacin, ampicillin, tetracycline, trimethoprim-sulfamethoxazole, cefepime, chloramphenicol, ceftazidime, cefotaxime, and ceftriaxone using the Kirby Bauer disc diffusion method. This study isolated 5 E. coli isolates (5/40; 12.5%), and 2 of them were confirmed as E. coli O157:H7. One isolate of E. coli O157:H7 was resistant against ampicillin and tetracycline, and one isolate was resistant against ampicillin, tetracycline, trimethoprim-sulfamethoxazole, and ceftazidime. The multi-drug resistance was identified only in 1 isolate of E. coli O157:H7.Houseflies collected from the food court have the potential to transmit antibiotic-resistant E. coli O157:H7 around the IPB campus.
Safety Risk Factors of Duck Meat Sold in Bogor City annisa, rizka fajri; Purnawarman, Trioso; Pisestyani, Herwin
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.101423

Abstract

With the increasing demand for duck meat in Bogor City, this poses a risk of the presence of pathogenic bacteria throughout the food supply chain. Traditional markets are one of the places that have a high risk of contamination with pathogenic bacteria that can endanger public health. This study aims to determine the presence of Salmonella and E. coli in duck meat sold in traditional markets in Bogor City and the risk factors that influence the presence of these pathogenic bacteria. This study was a cross sectional study with a total of 45 duck meat samples obtained from 3 traditional markets in Bogor City. Samples were taken to the SKHB IPB Veterinary Public Health Laboratory for microbiological testing. Risk factors that may affect the safety of duck meat were assessed using a questionnaire. The results showed that 13 samples (29%) were positive for Salmonella and 27 samples (60%) were positive for E. coli. The presence of Salmonella and E. coli in duck meat indicates cross-contamination during slaughter, evisceration and poor sanitation and hygiene practices. Continuous monitoring and reporting of the presence of Salmonella and E. coli is needed so that duck meat consumed by the public is safe for consumption.
Penerapan Sanitasi dan Higiene dalam Produksi Ikan Asap: Implikasi terhadap Keamanan Pangan dan Kesehatan Masyarakat Tina, Lymbran; Sudarnika, Etih; Ridwan, Yusuf; Sudarwanto, Mirnawati B.; Pisestyani, Herwin
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.105214

Abstract

Keamanan pangan merupakan aspek krusial dalam kesehatan masyarakat, terutama pada produk pangan yang diproses secara tradisional seperti ikan asap. Penelitian ini bertujuan mengevaluasi penerapan sanitasi dan higiene dalam produksi ikan asap di Kota Kendari serta mengkaji dampaknya terhadap keamanan pangan dan kesehatan masyarakat. Penelitian menggunakan desain observasional deskriptif dengan pengambilan sampel secara purposive pada 13 unit produksi ikan asap selama Juli sampai Oktober 2024. Data dikumpulkan melalui observasi langsung dan checklist kepatuhan terhadap standar Badan Pengawas Obat dan Makanan (BPOM). Hasil penelitian menunjukkan kurang dari sebagian produsen (48,4%) yang memastikan peralatan produksi tetap bersih dan terawat secara menyeluruh. Seluruh produsen (100%) menggunakan air bersih selama produksi, namun tanpa fasilitas pembersihan bahan baku yang memadai. Toilet yang memenuhi standar kebersihan hanya ditemukan pada 69,2% lokasi produksi, sementara tidak ada satu pun tempat sampah tertutup (0%). Evaluasi praktik higiene pekerja, didaptkan sebanyak 76,9% pekerja mencuci tangan sebelum dan sesudah bekerja, namun tidak ada pekerja yang mamakai alat pelindung diri (0%). Selain itu, seluruh lokasi produksi tidak memiliki program pembersihan dan sanitasi berkala (0%), dan terdapat hewan peliharaan di sekitar area produksi, yang dapat meningkatkan risiko kontaminasi mikroorganisme patogen. Kondisi ini menunjukkan bahwa industri ikan asap tradisional di Kendari belum memenuhi stadar sanitasi dan higiene yang baik sehingga memerlukan intervensi berbasis kesehatan masyarakat. Diperlukan edukasi kepada produsen mengenai pentingnya sanitasi dan higiene, peningkatan infrastruktur sanitasi, serta pengawasan lebih ketat dari pihak berwenang guna memastikan kepatuhan terhadap standar keamanan pangan. Kata kunci: keamanan pangan, higiene, kesehatan masyarakat, sanitasi, ikan asap
Co-Authors Abdul Zahid Adi Winarto Agik Suprayogi Ahmad, Hasniah Amalo, Gabryella Fransina Aminudin, Muhammad Rizki Andriani Andriani Anggraini, Raden Isma annisa, rizka fajri Ardilasunu Wicaksono Arifin B. Nugraha Bambang Pontjo Priosoeryanto Chaerul Basri Denny Lukman Denny Widaya Lukman Devi Yanti Sari Etih Sudarnika Fitria, Nisa Nurul Gabryella Fransina Amalo Hadri Latif Harsi D. Kusumaningrum Herwin Iccha Pradipta Patsiwi Indra Permana, Indra Irena Ivania Isdoni Bustaman Jannah, Zahratul Jannatun Delvia Katyusa Nandini Dyah Pitaloka koekoeh santoso Komala, Iyep Lymbran Tina Maya Shofa Mayang Rosana Mila Meha, Margie Pristiantine Milawarni Milawarni Mirnawati B. Sudarwanto Mirnawati Bachrum Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto MIRNAWATI SUDARWANTO Muhammad Pauzi Lubis Nadhear Nadadyanha Dannar Naoaki Misawa Nida, Lailatun Nugraha, Arifin Budiman Ramadhani, Nada Nursaffana Shodiq, Nadim Sudarwanto, Mirnawati Bachrum Supriyono, Supri Surachmi Setiyaningsih Surachmi Setyaninigsih Susanti, Oli Tangkonda, Elisabet Trioso Purnawarman Wahyudi, Ading Yunindika, Thufeil Yusuf Ridwan