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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis Indonesian Journal of Pharmaceutical Science and Technology Jurnal Farmasi Klinik Indonesia Acta Pharmaceutica Indonesia Indonesian Journal of Chemistry JSKK (Jurnal Sains Keolahragaan dan Kesehatan) Marine Research in Indonesia PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) JFIOnline Jurnal Ilmu Kefarmasian Indonesia JURNAL FARMASI GALENIKA Jurnal Farmagazine Narra J Pharmaceutical Sciences and Research (PSR) Molekul: Jurnal Ilmiah Kimia Jurnal Kefarmasian Indonesia Journal of Tropical Pharmacy and Chemistry Jurnal Farmasi Galenika

Sophi Damayanti

Institut Teknologi Bandung

Author-ID : 249183

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Optimasi Reaksi Transesterifikasi Minyak Kelapa Sawit (Elaeis guineensis Jacq.) dan Penetapan Kadar Metil Ester Asam Lemak Menggunakan Kromatografi Gas Sophi Damayanti; Vita Yuanita; Rahmana Emran Kartasasmita
Acta Pharmaceutica Indonesia Vol. 38 No. 1 (2013)
Publisher : School of Pharmacy Institut Teknologi Bandung

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Indonesia merupakan produsen minyak kelapa sawit (Elaeis guineensis Jacq.) terbesar di dunia. Pengolahan menjadi produk turunannya dapat meningkatkan nilai ekonomi minyak kelapa sawit. Salah satu produk turunannya adalah metil ester asam lemak yang memiliki banyak kegunaan dalam industri pangan, farmasi dan kosmetik. Metil ester asam lemak dihasilkan melalui reaksi transesterifikasi minyak kelapa sawit. Penelitian ini bertujuan untuk menentukan kondisi optimum reaksi transesterifikasi minyak kelapa sawit. Optimasi diamati berdasarkan penetapan kadar metil ester asam lemak secara semikuantitatif menggunakan metode kromatografi gas. Metil ester asam lemak disintesis dari minyak kelapa sawit menggunakan katalis natrium hidroksida dan kalium hidroksida masing-masing pada suhu 40, 50 dan 60 ºC selama 1, 2 dan 3 jam. Metil ester asam lemak yang diperoleh dinetralkan dan dicuci. Baku metil ester asam lemak disintesis dari asam palmitat. Kadar metil ester asam lemak ditentukan menggunakan kromatografi gas dengan kolom DB-Wax 30 m x 0,25 mm, detektor FID, dan kondisi oven pada suhu 50 ºC selama 1 menit, kemudian dinaikkan 25 ºC /menit sampai 200 ºC, lalu dinaikkan kembali 3 ºC /menit sampai 230 ºC dan ditahan selama 18 menit. Kadar asam lemak bebas dari minyak kelapa sawit dan metil ester asam lemak ditentukan dengan metode titrasi. Rata-rata bobot hasil reaksi transesterifikasi menggunakan katalis natrium hidroksida dan kalium hidroksida adalah masing-masing 84,63±2,63 dan 84,25±4,77 g. Rata-rata kadar asam lemak bebas metil ester asam lemak hasil reaksi transesterifikasi menggunakan katalis natrium hidroksida dan kalium hidroksida adalah 0,68±0,03% dan 0,65±0,03 %. Kondisi optimum reaksi transesterifikasi minyak kelapa sawit (Elaeis guineensis Jacq.) menggunakan katalis natrium hidroksida dan kalium hidroksida masing-masing adalah pada suhu 60 ºC selama 1 jam, sebesar 34,20% (b/v) dan pada suhu 50 ºC selama 2 jam, sebesar 32,40% (b/v).Kata kunci: Transesterifikasi, minyak kelapa sawit, metil ester asam lemak, kromatografi gas, katalis basa. AbstractIndonesia is the largest producer of crude palm oil (Elaeis guineensis Jacq.) in the world. Processing into derived products can increase the economic value of crude palm oil. One of the derived products is the fatty acid methyl esters that have many uses in the food, pharmaceuticals and cosmetic industry. Fatty acid methyl ester is produced through transesterification reaction of crude palm oil. The aim of this research is to obtain the optimum condition of transesterification reaction of crude palm oil. Optimization was observed by the assay of fatty acid methyl esters as semi-quantitative using gas chromatography method. Fatty acid methyl esters were synthesized from crude palm oil using catalysts sodium hydroxide and potassium hydroxide respectively at 40, 50 and 60 ºC for 1, 2 and 3 hours. The fatty acid methyl esters obtained were neutralized and washed. The standard of fatty acid methyl ester was made by palmitic acid synthesis. The assay of fatty acid methyl esters was carried out using gas chromatography equipped with 30 m x 0,25 mm DB-Wax column, flame ionization detector (FID), using oven temperature at 50 °C, 1 minute, 25 °C /minute to 200 °C, 3 °C /minute to 230 °C, 18 minutes and determination of free fatty acid content from crude palm oil and fatty acid methyl esters. The average mass of the result of the transesterification reaction using sodium hydroxide and potassium hydroxide as catalyst were 84.63±2.63 and 84.25±4.77 g, respectively. The average of free fatty acid content of fatty acid methyl ester synthesized using sodium hydroxide and potassium hydroxide as catalyst were 0.68±0.03 % and 0.65±0.03 %. The optimum conditions of the transesterification reaction of crude palm oil (Elaeis guineensis Jacq.) using sodium hydroxide and potassium hydroxide as catalyst were at 60 ºC for 1 hour and at 50 ºC for 2 hours, which were 34.20% (w/v) and 32.40% (w/v), respectively.Keywords: Transesterification, crude palm oil, fatty acid methyl ester, gas chromatography, base catalyst.

Pengembangan Metode Penentuan Kadar ZnO dalam Bedak Wajah Menggunakan Spektroskopi Serapan Atom Sophi Damayanti; Eko Prayitno; Farel Fahrizal; Adi Suwandi
Acta Pharmaceutica Indonesia Vol. 43 No. 1 (2018)
Publisher : School of Pharmacy Institut Teknologi Bandung

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Seng (Zn) dalam bentuk seng oksida (ZnO) merupakan bahan tabir surya yang terdapat dalam berbagai kosmetik antara lain dalam produk bedak. ZnO apabila berpenetrasi ke dalam kulit dalam jumlah berlebih dapat menyebabkan terjadinya fotosensitisasi sehingga penggunaannya di dalam kosmetik dibatasi oleh pemerintah. Tujuan dari penelitian ini adalah untuk mengembangkan metode penentuan kadar Zn dalam bedak dan menggunakannya untuk penentuan kadar Zn dalam beberapa sampel bedak yang beredar di Indonesia dengan menggunakan spektroskopi serapan atom (SSA). Persamaan linearitas metode dari hasil pengukuran larutan standar Zn yaitu y = 0,0088x + 0,0029 dengan koefisien regresi 0,9999. Metode analisis memiliki Limit of Detection (LOD) dan Limit of Quantification (LOQ) sebesar 0,101 µg/mL dan 0,306 µg/mL. Pengukuran kadar ZnO pada 12 sampel bedak yang beredar di pasaran menunjukkan hasil yang beragam dengan rentang 1,74 "“ 25,76 %, dari hasil tersebut diketahui bahwa 2 dari 12 sampel mengandung ZnO dengan kadar yang melebihi persyaratan Badan Pengawas Obat dan Makanan (BPOM) Republik Indonesia.

VERIFIKASI METODE DAN PENENTUAN KADAR LAKTOSA DALAM SAMPEL SUSU YANG BERPERISA MENGGUNAKAN KROMATOGRAPI CAIR KINERJA TINGGI Sophi Damayanti
JSKK (Jurnal Sains Keolahragaan dan Kesehatan) Vol 4 No 1 (2019)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/jskk.2019.4.1.2

Laktosa merupakan gula disakarida dari glukosa dan galaktosa dalam susu. Sebagai sumber sumber gizi, susu dipilih oleh atlet untuk dikonsumsi. Namun demikian. laktosa dapat menyebabkan laktosa intoleransi, oleh karena itu kandungannya perlu ditentukan. Tujuan dari penelitian ini adalah untuk verifikasi metode dan penentuan kadar laktosa dalam susu yang berperisa dengan menggunakan kromatografi cair kinerja tinggi (KCKT). Sampel susu diencerkan dengan asetonitril(1:1) dan dilarutkan hingga 100mL dengan air. Susu kemudian difiltrasi dengan 0.45µm filter membrane. laktosa kemudian dianalisis menggunakan KCKT detektor indeks bias, kolom fase terikat NH2, 10µm (250µm Ã— ID 4.6mm), dan suhu kolom pada 25oC, fase gerak asetonitril dan air (65:35) dengan laju alir adalah 1,0mL/min. Verifikasi metode menunjukkan linearitas dengan koefisien korelasi, r2 = 0.9997 dan persamaan garis y=106434,5500x-77704,1667. Koefisien fungsi regresi yang dihitung (Vx0) untuk laktosa yaitu 0.376. Pengujian presisi menghasilkan koefisien variansi untuk inter dan intraday adalah 0,3844 dan 1,6758. Persentase perolehan kembali untuk laktosa dengan metode standar adisi berada dalam rentang 99,018-101.408 %. Sampel 1 mengandung laktosa 1,277±0,283 dan sampel 2 mengandung 1,094±0,013 %. Sebagai kesimpulan, kandungan laktosa dalam susu berperisa dapat ditentukan dengan menggunakan kromatografi cair kinerja tinggi dengan detektor indek bias.

Potensi Ketergantungan Mahasiswa Terhadap Konsumsi Minuman Berenergi Kusnandar Anggadiredja; Triantri Kartika Putri; Sophi Damayanti
JSKK (Jurnal Sains Keolahragaan dan Kesehatan) Vol 6 No 1 (2021)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/jskk.2021.6.1.6

Minuman berenergi merupakan suplemen makanan yang terdiri dari multivitamin, makronutrien, taurin, kafein dengan tambahan herbal seperti ginseng dan jahe dengan bentuk sediaan cairan dalam kemasan botol, serbuk dan tablet yang dilarutkan menjadi minuman, yang dalam setiap kemasannya mengandung energi minimal 100 kkal. Penggunaannya adalah untuk menambah tenaga, stimulasi metabolisme, memelihara kesehatan dan stamina, yang diminum saat bekerja keras atau setelah olahraga. Konsumsi minuman berenergi meningkat sejak merk minuman berenergi terkenal mulai diperdagangkan tahun 1997. Penelitian di Amerika menunjukkan, 51% mahasiswa dari 496 mahasiswa yang diteliti mengonsumsi minuman berenergi lebih dari satu kali dalam sebulan. Dilihat dari kepopuleran minuman berenergi pada mahasiswa, diperlukan adanya ukuran untuk menilai potensi ketergantungannya. Penelitian ini bertujuan mempelajari mengenai potensi ketergantungan mahasiswa terhadap minuman berenergi. Penelitian ini adalah penelitian deskriptif dengan pengumpulan data berdasarkan pengisian kuesioner yang diisi oleh responden pada Maret 2017 sampai Juli 2017. Data dianalisis dan untuk memperoleh informasi derajat ketergantungan tiap responden menggunakan kriteria Diagnostic and Statistical Manual of Mental Disorder V (DSM V). Jumlah total responden adalah 184 responden. Hasil menunjukkan 58% responden adalah perempuan dan 42% responden adalah laki-laki. Dari 6 merk minuman berenergi, merk E adalah minuman yang paling banyak dikonsumsi. Dua puluh tujuh persen responden mengonsumsi minuman berenergi satu kali dalam sebulan. Berdasarkan kriteria DSM V, 53% responden tidak mengalami ketergantungan, 30% mengalami ketergantungan ringan, 10% mengalami ketergantungan sedang dan 7% mengalami ketergantungan berat. Walaupun lebih dari setengah jumlah responden tidak mengalami ketergantungan, namun ketergantungan minuman berenergi pada mahasiswa tetap harus diperhatikan karena terdapat 47% potensi ketergantungan minuman berenergi pada mahasiswa yang diteliti.

Interaction Study, Synthesis and Characterization of Molecular Imprinted Polymer Using Functional Monomer Methacrylate Acid and Dimethylamylamine as Template Molecule Saeful Amin; Sophi Damayanti; Slamet Ibrahim
JURNAL ILMU KEFARMASIAN INDONESIA Vol 16 No 1 (2018): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

The research related to the interaction study, synthesis and characterization of molecular imprinted polymer using dimethylamylamine (DMAA) as the template molecule and the functional monomer methacrylate acid has been conducted. Molecular Imprinted Polymer (MIP) is a separation method made by the molecule template in the polymer matrix followed by removing the template molecule by washing for giving the permanent framework groove. The MIP was made by mixing the DMAA as the template molecule, with the methacrylate acid as the functional monomer, and the ethylene glycol dimethacrylate (EGDMA) as the crosslinker with the ratio 1:6:20. Porogen solvents used were the chloroform and the initiator azobisisobutyronitrile (AIBN). The crystal MIP and the NIP without the DMAA were characterized using Infrared Spectrophotometer (FTIR), and the result showed that there have been differences among the MIP, the NIP and the MIP after being extracted. The characterization using Scanning Electron Microscope (SEM) showed that the NIP as the comparison having flat morphology, while the MIP having irregular morphology and less pores. Then the MIP after being extracted has irregular, rough morphology and a lot of pores. The result reveals the interaction between the DMAA and the methacrylate acid that is the hydrogen bonded with the Gibbs free energy obtained is -5.434 j/mol. The imprinting factor of 2,353 is obtained. The highest desorption descending capacity is chloroform with the MIP 738% better. For the MIP and NIP methanol, it is found that the MIP is 123% better. Then the MIP which is desorbed by the chloroform is better 602% than the MIP resorbed by the methanol, and the ethyl acetate cannot desorb the DMAA.

PREDIKSI TOKSISITAS SENYAWA ANTIOKSIDAN ALAMI DAN ANALISIS INTERAKSINYA TERHADAP RESEPTOR VEGF-1 MENGGUNAKAN METODE MOLECULAR DOCKING Dina Pratiwi S.Si, M.Si; Muhammad Insanu; Sophi Damayanti
Jurnal Farmagazine Vol 1, No 1 (2014): Jurnal Farmagazine
Publisher : STF Muhammadiyah Tangerang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47653/farm.v1i1.40

Antioksidan merupakan zat yang mampu melindungi pangan dari proses oksidasi. Penggunaan antioksidan sintetik telah menjadi perdebatan dikarenakan efek negatif yang ditimbulkannya. Antioksidan alami dari tumbuhan dapat menjadi senyawa potensial sebagai bahan tambahan pangan alternatif. Namun belum banyak penelitian mengenai efek toksik antioksidan alami. Penelitian ini dilakukan untuk memperoleh prediksi toksisitas 300 senyawa antioksidan alami tumbuhan menggunakan metode in silico dan analisis interaksinya dengan reseptor VEGF-1 melalui metode molecular docking. Prediksi toksisitas senyawa dilakukan menggunakan perangkat lunak T.E.S.T, Toxtree, ADMET predictor, dan QSAR Toolbox. Analisis senyawa uji dengan reseptor VEGF-1 melalui molecular docking dilakukan menggunakan perangkat lunak Autodock 4.2.6. Hasil analisis molecular docking terlihat bahwa terdapat interaksi antara senyawa uji dengan reseptor melalui adanya kemiripan residu asam amino yang berdekatan seperti pada senyawa pembanding dan adanya ikatan hidrogen. Interaksi dengan reseptor VEGF-1 pada uji karsinogenitas menunjukkan asam rosmarinat dan kapsaisin memiliki energi bebas ikatan terendah dengan nilai -8,54 dan -8,11 kcal/mol. Kata kunci: antioksidan alami, molecular docking, VEGFR-1

SIMULTANEOUS DETERMINATION OF PARACETAMOL AND IBUPROFENE MIXTURES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Sophi Damayanti; Slamet Ibrahim; Kurnia Firman; Daryono H Tjahjono
Indonesian Journal of Chemistry Vol 3, No 1 (2003)
Publisher : Universitas Gadjah Mada

Analytical method for the determination of paracetamol and ibuprofene mixtures has been developed by High Performance Liquid Chromatography using C-18 column and acetinitrile - phosphate buffer pH = 4.5 (75:25) containing 0.075% sodium hexanesulfunate as a mobile phase. The detector was set at 215 nm. Using such conditions, retention time for paracetamol and ibuprofen was 4.89 and 7.11 min, respectively. The recovery for paracetamol and ibuprofen was found to be 101.07± 0.73% and 102.02 ± 1.58%, respectively. The detector limits of the method was 1.30 and 1.60 μg/mL with the relative standard deviation (RSD) 0.74 and 1.52% for paracetamol and ibuprofen, respectively.

Molecular Docking, Dynamics Simulation, and Scanning Electron Microscopy (SEM) Examination of Clinically Isolated Mycobacterium tuberculosis by Ursolic Acid: A Pentacyclic Triterpenes Dian Ayu Eka Pitaloka; Sophi Damayanti; Aluicia Anita Artarini; Elin Yulinah Sukandar
Indonesian Journal of Chemistry Vol 19, No 2 (2019)
Publisher : Universitas Gadjah Mada

The purpose of this study was to analyze the inhibitory action of ursolic acid (UA) as an antitubercular agent by computational docking studies and molecular dynamics simulations. The effect of UA on the cell wall of Mycobacterium tuberculosis (MTB) was evaluated by using Scanning Electron Microscopy (SEM). UA was used as a ligand for molecular interaction and investigate its binding activities to a group of proteins involved in the growth of MTB and the biosynthesis of the cell wall. Computational docking analysis was performed by using autodock 4.2.6 based on scoring functions. UA binding was confirmed by 30 ns molecular dynamics simulation using gromacs 5.1.1. H37Rv sensitive strain and isoniazid-resistant strain were used in the SEM study. UA showed to have the optimum binding affinity to inhA (Two-trans-enoyl-ACP reductase enzyme involved in elongation of fatty acid) with the binding energy of -9.2 kcal/mol. The dynamic simulation showed that the UA-inhA complex relatively stable and found to establish hydrogen bond with Thr196 and Ile194. SEM analysis confirms that UA treatment in both sensitive strain and resistant strain affected the morphology cell wall of MTB. This result indicated that UA could be one of the potential ligands for the development of new antituberculosis drugs.

In Silico Study on Interaction and Preliminary Toxicity Prediction of Eleutherine americana Components as an Antifungal and Antitoxoplasmosis Candidate Sophi Damayanti; Nadiyah Athifah Salim Martak; Benny Permana; Adi Suwandi; Rika Hartati; Indra Wibowo
Indonesian Journal of Chemistry Vol 20, No 4 (2020)
Publisher : Universitas Gadjah Mada

Red bulbs of Eleutherine americana (Aubl.) Merr. ex K. Heyne has been known for its high content of naphthoquinones that have antifungal and antiparasitic activities. In this research, in silico interaction study was performed between 31 compounds reported to be found in E. americana with the selected target proteins for antifungal and antitoxoplasmosis activity using the molecular docking method. An ORPs (OSBP-related proteins), Osh4 (PDB ID: 1ZHX), and N-myristoyltransferase (Nmt, PDB ID: 1IYL) were used as the antifungal target proteins. Toxoplasma gondii purine nucleoside phosphorylase (TgPNP, PDB ID: 3MB8) and calcium-dependent protein kinase-1 (TgCDPK1, PDB ID: 4M84) were used as antitoxoplasmosis target proteins. Three-dimensional structures of the test compounds were made and optimized using GaussView 6.0 and Gaussian 09W. The target proteins were prepared using the Discovery Studio 2016 Program. Aquatic toxicity prediction as the preliminary assessment of the safety of the compounds was performed using ECOSAR v2.0. The results suggest that the compound having both the smallest free binding energy compared with positive control and other test compounds and low predicted toxicity is β-sitosterol with a free binding energy of ‒11.55 and ‒11.18 kcal/mol towards Osh4 and Nmt and ‒8.06 and ‒10.29 kcal/mol towards TgPNP and TgCDPK1, respectively.

Simple Method of 9,10-Anthraquinone Assay in Eleutherine americana (Aubl.) Merr. ex K. Heyne using High-Performance Liquid Chromatography Sophi Damayanti; Samuel Gunadi Tanusondjaja; Benny Permana; Rika Hartati; Dian Ayu Eka Pitaloka; Indra Wibowo
Indonesian Journal of Chemistry Vol 21, No 6 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.64701

Eleutherine americana (E. americana) is a medicinal plant commonly found on the island of Borneo, Indonesia. This plant is known to have several biological activities. However, anthraquinone residues are generally present as contaminants. This study was aimed to develop a method of determining the levels of 9,10-anthraquinone in plant extracts and fractions using High Performance Liquid Chromatography (HPLC). The research aims to optimize the mobile phase, the system suitability test, and the system validation. The optimal mobile phase was acetonitrile:distilled water 1:1 v/v with a flow rate of 1.25 mL/min. The validation result shows that the linearity was obtained with a correlation coefficient (r) of 0.9995 and an r2 coefficient of 0.9991. The estimated limits for detection and quantification values were 0.178 and 0.594 µg/mL, respectively. In the intraday and inter-day accuracy test, the coefficient of variance for reference was 0.627 and 0.774, while the results for the sample were 2.966 and 2.658. The percentage recovery rate for reference was between 98.976–101.452%, and for the sample, the result was 89.191–94.667%. The average 9,10-anthraquinone content in the acetate fraction of E. americana plant was 9.799 µg/g ± 5.243.

Co-Authors Adi Suwandi Adi Suwandi Ahsogan, Kanaka L. Aluicia Anita Artarini Anjar Hermadi Saputro Bambang Gunawan Choong Chie Weng Daryono H Tjahjono Dian Ayu Eka Pitaloka Dina Pratiwi S.Si, M.Si Dwiyanti, Jessica Eko Prayitno Elin Yulinah Sukandar Engrid Juni Astuti Farel Fahrizal Fathurohman, Mochamad Fitria Muharini Framesti Frisma Sriarumtias Galih, Wening Gunawan, Bambang Hadi, Daryono Hariyanti Hariyanti HENI RACHMAWATI Indra Maulana` Indra Maulana Indra Topik Maulana Indra Wibowo Indra Wibowo Irma M. Puspitasari Irma M. Puspitasari, Irma M. Iskandar, Fery Julianti, Elin Julianti, Elin Kanaka L. Ahsogan Kartasasmita, Rahmana Emran Khonsa Khonsa Kurnia Firman Kusnandar Anggadiredja LIA AMALIA Mardiana Mardiana Mardiana, Mardiana Marlia Singgih Marlia Singgih Masdiana Tahir Muhammad Ali Zulfikar Muhammad Insanu Muharini, Fitria Nadiyah Athifah Salim Martak Nur Mita Nurwulan, Sita Permana, Benny Permana, Benny Permatasari, Fitri A. Pinanga, Yangie Dwi Marga Rahmana Emran Kartasasmita Rahmana Emran Kartasasmita Rasida, Rima Reyaan, Irianti Bahana Maulida Rika Hartati Saeful Amin Saeful Amin, Saeful Samuel Gunadi Tanusondjaja Sasanti Tarini Darijono Slamet Ibrahim Slamet Ibrahim - Sriarumtias, Framesti Frisma Sukrasno Sukrasno Sukraso Sukraso Tasia Amelia Tjahyono Tjahyono Triantri Kartika Putri Vita Yuanita Wanti Megawati Weng, Choong Chie Wibowo, Indra Wijayanti, Ni PAD. Yuanita, Vita

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