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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Sain Veteriner Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Biotechnology UNEJ e-Proceeding SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Biosaintifika: Journal of Biology & Biology Education Jurnal Pengabdian Masyarakat Kesehatan Amerta Nutrition Journal of Aquaculture and Fish Health Indian Journal of Forensic Medicine & Toxicology Berkala Penelitian Hayati Exact Paper In Compilation (EPIC) Jurnal Kedokteran Hewan Borneo Journal of Pharmacy Journal of Fisheries & Marine

Win Darmanto

Departement Of Biology, Faculty Of Science And Technology, Airlangga University, Indonesia

Author-ID : 249947

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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PROFIL DISTRIBUSI INOS DAN KADAR NO PANKREAS TIKUS DIABETES MELITUS HASIL INDUKSI MLD-STZ PASCA PEMBERIAN EKSTRAK ETANOL TEMUGIRING (Curcuma heyneana) Betti Lukiati; Aulanni’am a; Win Darmanto
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan menguji pengaruh ekstrak etanol temugiring terhadap distribusi inducible nitric oxide synthase (iNOS) dan kadar nitrogen oksida (NO) pada pankreas tikus diabetes hasil induksi multiple low dose-streptozotocin (MLD-STZ). Tiga macam dosis ekstrak etanol temugiring, yaitu 36, 72, dan 108 mg/kg bobot badan diberikan secara oral untuk terapi tikus diabetes hasil induksi MLD-STZ. Data distribusi iNOS dan kadar NO dianalisis dengan analisis varians jalur tunggal yang dilanjutkan dengan uji Duncan (α=0,05). Hasil analisis menunjukkan bahwa ekstrak etanol temugiring berpengaruh sangat nyata (P0,01) terhadap penurunan distribusi iNOS dan kadar NO pada pankreas tikus diabetes hasil induksi MLD-STZ. Dosis 72 mg/kg bobot badan ekstrak etanol temugiring merupakan dosis optimal untuk terapi tikus diabetes hasil induksi MLD-STZ. Disimpulkan bahwa ekstrak etanol temugiring dapat menurunkan distribusi iNOS dan kadar NO pankreas tikus penderita diabetes melitus.

APLIKASI BERBASIS ANDROID DALAM PEMETAAN DAN IDENTIFIKASI KONTAK PASIEN TB PARU DI KABUPATEN JOMBANG Win Darmanto; Sri Puji Astuti Wahyuningsih; Suhendra Agung Wibowo
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 7, No 2 (2023): June
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v7i2.15203

ABSTRAKTuberkulosis masih menjadi permasalahan yang belum bisa teratasi bagi seluruh dunia. Banyak upaya yang telah dilakukan dalam penanganan dan belum mendapatkan hasil yang diharapkan. Salah satu penyebab kesulitan dalam penanganan tuberkulosis paru adalah karena pasien tersebar di wilayah yang luas pada saat menjalani pengobatan dan belum terpetakan. Melalui bantuan kader kesehatan penulis mencoba untuk memetakannya. Metode pengabdian masyarakat yang digunakan adalah berupa pendidikan dan pelatihan dalam menggunakan aplikasi berbasis android dalam pemetaan dan identifikasi kontak pasien TB Paru di kabupaten Jombang melalui kerjasama dengan TB Care Yayasan Bhanu Yasa Sejahtera (YABHYSA) kabupaten Jombang dan diikuti oleh 40 orang kader pendeteksi dan pengendali TB dibawah naungan TB Care YABHYSA Jombang. Kegiatan diawali dengan penyamaan persepsi pada tim pengabdian masyarakat, kemudian kader sebagai sasaran dikumpulkan dalam satu ruangan untuk menerima penjelasan tentang aplikasi sekaligus mempraktekkan bagaimana cara menggunakannya. Hasil pelatihan menunjukkan nilai post test 100% responden lebih baik daripada pre test. Aplikasi diserahkan kepada kader untuk dapat digunakan dalam pemetaan pasien TB Paru. Berdasarkan hasil post test dapat disimpulkan bahwa kader telah mengerti dan memahami cara penggunaan aplikasi sehingga siap untuk memetakan pasien TB Paru di Kabupaten Jombang. Kata kunci: pencegahan TB; aplikasi, pemetaan. ABSTRACTTuberculosis is still an unresolved problem for the whole world. Many efforts have been made in handling and have not received the expected results. One of the causes of difficulties in treating pulmonary tuberculosis is because patients are spread over a large area at the time of treatment and have not been mapped. Through the help of health cadres the author tries to map it. The community service method used is in the form of education and training in using an android-based application in mapping and identifying contacts of pulmonary TB patients in Jombang district through collaboration with the Bhanu Yasa Sejahtera Foundation (YABHYSA) Jombang district TB Care and attended by 40 TB detection and control cadres under the auspices of TB Care YABHYSA Jombang. The activity began with the equalization of perceptions on the community service team, then the cadres as targets were gathered in one room to receive an explanation about the application as well as practice how to use it. The results of the training showed that the post test scores of 100% of the respondents were better than the pre test. Applications are submitted to cadres to be used in mapping pulmonary TB patients. Based on the results of the post test, it can be concluded that the cadres understand and understand how to use the application so they are ready to map pulmonary TB patients in Jombang Regency. Keywords : TB prevention; application; mapping

Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus Sri Puji Astuti Wahyuningsih; Manikya Pramudya; Intan Permata Putri; Nadyatul Ilma Indah Savira; Dwi Winarni; Listijani Suhargo; Win Darmanto
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11284

Okra (Abelmoschus esculentus) is reported to have various biological functions such as antioxidant, anticancer and anti-inflammation. However, only few studies have been reported immunomodulatory activities of okra to prevent disease caused by bacteria infection. In this study, the immunomodulatory activities of polysaccharides from okra pods were investigated further through the spleen weight and B-lymphocytes proliferation in mice infected by Staphylococcus aureus. Okra polysaccharides were obtained by water extraction and ethanol precipitation. Okra polysaccharides with doses of 25, 50, 75, and 100 mg/kg BW were orally administrated to mice with or without Staphylococcus aureus infection. Spleen weight was evaluated in both treatment group and control group. B-lymphocytes proliferation was evaluated by MTT assay using LPS induction. Results showed that okra polysaccharide at the doses of 50, 75, and 100 mg/kg increased spleen weight (p 0.05) significantly. While at the dose of 75 and 100 mg/kg, it increased the B-lymphocytes proliferation (p 0.05) significantly. There is positive correlation between the spleen weight and B-lymphocytes proliferation by 73.3%. These result reveal that okra polysaccharide could improve the immune response and be utilized as a novel candidate of neutraceutical.

Effect on Polysaccharide Krestin from Coriolus versicolor Extract on Phagocytic Activity and Capacity of Mus musculus Exposed by Pseudomonas aeruginosa Sri Puji Astuti Wahyuningsih; Nadyatul Ilma Indah Savira; Win Darmanto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6957

Pseudomonas aeruginosa is an opportunistic bacterium that causes infections in human. The wall cell of its bacteria contains lipopolysaccharide as virulency factors to protect it from human immunity. Lipopolysaccharide can inhibit phagocytosis in the body. Polysaccharide krestin (PSK) from Coriolus versicolor extract contains β-glucan that can increase phagocytic activity and capacity. This research aimed to identify the effect on polysaccharide krestin from C. versicolor extract on phagocytic activity and capacity of mice exposed by P. aeruginosa. The design of this research was experimental design. There were six treatment groups. The phagocytic activity and capacity were counted on slide smears of mice peritoneal fluid. The data was analyzed by using one way ANOVA. The results of the phagocytic activity and capacity showed that PSK was added before exposure (P1) or after exposure (P2) or before-after exposure (P3) had potential to increase phagocytic activity and capacity. The conclusion of the research was that adding polysaccharide krestin either before exposure or after exposure or both of them could increase phagocytic activity and capacity. The benefits of this research to development of science are expected to reduce human infection and to utilize natural ingredients as immunomodulator.How to CiteWahyuningsih, S. P. A., Savira, N. I. I, Darmanto, W. (2016). Effect on Polysaccharide Krestin from Coriolus versicolor Extract on Phagocytic Activity and Capacity of Mus musculus Exposed by Pseudomonas aeruginosa. Biosaintifika: Journal of Biology Biology Education, 8(3), 308-313.

Kejadian Kelainan Vertebra Ikan Nila (Oreochromis niloticus) pada Media Penetasan Salinitas yang Berbeda [Vertebrae Malformation Tilapia Fish (Oreochromis niloticus) on Different Media Hatching Saline] Lailatul Lutfiyah; Rr Juni Triastuti; Endang Dewi Masithah; Win Darmanto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11312

Abstract Tilapia fish belonged to the euryhaline fish, which has wide range tolerance in salinity (freshwater euryhaline fish has salinity tolerance >8 ppt). Incidence of fish skeletal abnormalities could be the response of the environment influence such as salinity, temperature and hypoxia. This research uses five treatments : 0,5 10, 15, 20 ppt. The results showed that the highest abnormalities found on the treatment of 15 ppt and 20 ppt, while the lowest for the treatment of abnormalities of 10 ppt. The best result to be used in the brackish nila aquaculture is 10-15 ppt in salinity.

Improving Case Detection Rate( Cdr) By “Simatb” As Tuberculosis Screening Application Of High Risk Groups In Wonosalam: Upaya Peningkatan Case Detection Rate (Cdr) Tuberkulosis Di Kabupaten Jombang Melalui “Simatb” Sebagai Aplikasi Skrining Tuberkulosis Pada Faktor Beresiko Di Wonosalam Jombang farach; darmanto, Win; Pujiastuti Wahyuningsih , Sri; Puspita Sari, Evi; Susanto, awaluddin
Jurnal Pengabdian Masyarakat Kesehatan Vol. 9 No. 2 (2023): JPM | September 2023
Publisher : UPPM - STIKES Pemkab Jombang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33023/jpm.v9i2.1762

Capaian Case detection rate (CDR) TBC di Kabupaten Jombang Tahun 2021 belum mencapai target yang diinginkan. Sistem rujukan belum optimal, kurangnya keikutsertaan masyarakat dalam menemukan penderita, masyarakat memandang penyakit TBC dapat menggambarkan stigma negative dan mengakibatkan efek rasa malu dan terisolir bagi penderitanya. Kegiatan pengabdian masyarakat yang bertujuan untuk mengetahui kasus baru TB fakto resiko yang berada di Wonosalam sebagai upaya peningkatan CDR. Metode pelaksanaan kegiatan dilakukan (1) Persiapan meliputi penyuluhan penularan dan bahaya penyakit TBC (2) Pelaksanaan meliputi pemeriksaan kesehatan pada semua factor beresiko dan skrining untuk penemuan kasus tuberculosis melalui investigasi kontak dengan sasarannya adalah orang yang memiliki resiko. Hasil kegiatan menunjukkan 100% jumlah sputum beresiko tidak mengandung mikobakteri tuberculosis dan penggunaan aplikasi simaTB sangat berguna dalam kegiatan skrining TB selanjutnya. Kesimpulan yang didapatkan adalah Jumlah sputum factor beresiko di Wonosalam sebanyak 36 sampel dan menghasilkan negative mikobakterium Tuberkulosis.Aplikasi simaTB dapat digunakan sebagai upaya peningkatan case detection rate (CDR) tuberkulosis di kabupaten jombang melalui “simatb” sebagai aplikasi skrining tuberkulosis pada faktor beresiko di wonosalam jombang

THE EFFECTIVENESS OF HERBAL MOSQUITO COILS "MORIZENA” AGAINST Aedes Aegypti DEATH Susilowati, Rina Priastini; Darmanto, Win; Aminah, Nanik Siti
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 2 (2018)
Publisher : Institute of Topical Disease Universitas Airlangga

It has been successfully found new herbal mosquito coils called "Morizena” made from Passiflora foetida leaf extract (40%), Chrysantemum cinerariaefolium flowers seed extract (40%), essential oils of leaves-stems of Cymbopogon nardus (20%). The herbal mosquito coils are more effective to kill Aedes aegypti compared to commercial synthetic mosquito coils made from Transfluthrin. Treatment of exposure to herbal mosquito coils "Morizena” on Aedes aegypti for 8 hours/day with concentration 500 ppm (P1), 1000 ppm (P2), 2000 ppm (P3), 3000 ppm (P4), 4000 ppm (P5) and treatment of exposure to synthetic mosquito coils Transfluthrin 2500 ppm (K1) as a positive control, and without treatment of exposure to mosquito coils (K0) as a negative control. Experimental animals used were adult Ae. aegypti mosquito with 25 mosquitoes in each treatment. Experimental design used is one-way Anova test with linear regression to calculate its LC50 and LC90. The results of test to herbal mosquito coils "Morizena” given for 8 hours/day obtained mortality of Ae. aegypti by 92% to a concentration of 3000 ppm (P4) and 100% to a concentration of 4000 ppm (P5) and 100% for test to synthetic mosquito coils transfluthrin 2500 ppm (K1). Ae. aegypti LC50 and LC90 value for treatment of exposure to herbal mosquito coils "Morizena” are 999 ppm and 2977 ppm. Treatment of herbal mosquito coils "Morizena” with graded doses up to 4000 ppm and synthetic mosquito coils Transfluthrin 2500 ppm causing an increase in the enzyme acetylcholinesterase activity of Ae. aegypti. The conclusion is based on the Ae. aegypti LC90 value is 2977 ppm, which means the effective dose of herbal mosquito coils "Morizena” to kill Ae. aegypti is 2977 ppm.

PROFIL DISTRIBUSI INOS DAN KADAR NO PANKREAS TIKUS DIABETES MELITUS HASIL INDUKSI MLD-STZ PASCA PEMBERIAN EKSTRAK ETANOL TEMUGIRING (Curcuma heyneana) Lukiati, Betti; a, Aulanniam; Darmanto, Win
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.343

Penelitian ini bertujuan menguji pengaruh ekstrak etanol temugiring terhadap distribusi inducible nitric oxide synthase (iNOS) dan kadar nitrogen oksida (NO) pada pankreas tikus diabetes hasil induksi multiple low dose-streptozotocin (MLD-STZ). Tiga macam dosis ekstrak etanol temugiring, yaitu 36, 72, dan 108 mg/kg bobot badan diberikan secara oral untuk terapi tikus diabetes hasil induksi MLD-STZ. Data distribusi iNOS dan kadar NO dianalisis dengan analisis varians jalur tunggal yang dilanjutkan dengan uji Duncan (=0,05). Hasil analisis menunjukkan bahwa ekstrak etanol temugiring berpengaruh sangat nyata (P0,01) terhadap penurunan distribusi iNOS dan kadar NO pada pankreas tikus diabetes hasil induksi MLD-STZ. Dosis 72 mg/kg bobot badan ekstrak etanol temugiring merupakan dosis optimal untuk terapi tikus diabetes hasil induksi MLD-STZ. Disimpulkan bahwa ekstrak etanol temugiring dapat menurunkan distribusi iNOS dan kadar NO pankreas tikus penderita diabetes melitus.

Evaluation of Mangrove, Acanthus ilicifolius Aqueous Extract on Inhibition of Dengue Virus Replication: In Vitro and Molecular Docking Approach Wiradana, Putu Angga; Wiartini, Ni Wayan Ayu Wiartini; Sucipto, Teguh Hari; Herdiansyah, Mochammad Aqilah; Permatasari, Anak Agung Ayu Putri; Widhiantara, I Gede; Sari, Ni Kadek Yunita Sari; Darmanto, Win; Panjaitan, Novaria Sari Dewi Panjaitan; Kusala, Muhammad Khaliim Jati Kusala
Indonesian Journal of Tropical and Infectious Disease Vol. 13 No. 3 (2025)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v13i3.71217

Dengue virus (DENV) infection is a worldwide health risk, presenting severe symptoms with possibly lethal consequences. The search for effective antiviral drugs against DENV has largely focused on natural products. Mangrove, Acanthus ilicifolius, has been empirically shown to exhibit various biological activities and good safety profiles. The purpose of this investigation was to examine the antiviral activity of A. ilicifolius aqueous extract against DENV, and the way it functions of action of its bioactive components function was explored utilizing an in vitro and molecular docking method. Aqueous extract of A. ilicifolius leaves was made, and its therapeutic potential was examined using half cytotoxic concentration (CC50) and effective concentration (EC50) on Vero cells using the MTT method. The selectivity index (SI) value was determined from the ratio of CC50 and EC50. A total of 12 ligand compounds reported to be contained in the extract were prepared for molecular docking using AutoDock Vina, followed by in silico drug-like potential. A. ilicifolius water extract achieved the greatest Vero cell viability rate of 159.80% at a concentration of 31.25 μg/mL. A. ilicifolius extract at 100 μg/mL demonstrated the greatest DENV-3 inhibitory rate of 79.69%. The CC50 value was 78.55 μg/ml, and the EC50 value was 64.37 μg/mL. The SI value calculated from the ratio of CC50 to EC50 was 1.22. Molecular docking identified stigmasterol, stigmasterol-3-O-β-d-glucopyranoside, and quercetin as the primary compounds with the greatest docking values (-7.9 kcal/mol, -7.6 kcal/mol, and -7.7 kcal/mol, respectively). The findings of this study demonstrate the anti-DENV capability of the A. ilicifolius extract compound, which can limit viral multiplication via biological agents that interact with virus attachment within host cells, with an SI value of 1.22.

Efektivitas Ekstrak Etanol Umbi Decalobanthus mammosus (Lour.) A.R.Simões & Staples Terhadap Makrofag Paru dan Hati Mencit yang Diinduksi Mycobacterium tuberculosis: The Effectiveness of Ethanol Extract Decalobanthus mammosus (Lour.) A.R.Simões & Staples Tubers Against Mycobacterium tuberculosis Induced Lung and Liver Macrophages in Mice Evy Diah Woelansari; Win Darmanto; Firli Rohmah Primula Dewi; Khambali Khambali
Amerta Nutrition Vol. 10 No. 1SP (2026): AMERTA NUTRITION SUPPLEMENTARY EDITION
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/amnt.v10i1SP.2026.52-57

Background: Tuberculosis (TB) cases in Indonesia rank third in the highest number of cases. Incomplete TB treatment causes drug resistance. Ethanol extract of Decalobanthus mammosus (Lour.) A.R.Simões & Staples, tubers has the potential as an immunomodulator and antibacterial. Objectives: This research purpose to decide potency of ethanol essence of Decalobanthus mammosus (Lour.) A.R.Simões & Staples tubers on lung and liver macrophage cells in mice induced by Mycobacterium tuberculosis. Methods: Laboratory experimental research using posttest only with control group. The research sample using Mus muculus mice was divided into control and experimental groups, with the administration of extract doses of 50 mg/kgBW, 100 mg/kgBW and 150 mg/kgBW, and injection of Mycobacterium tuberculosis. Result: The results showed that Decalobanthus mammosus extract dose of 150 mg/kgBW, macrophages were able to overcome Mycobacterium tuberculosis infection, with the lowest number of necrotic cells in the lungs. activation of liver macrophages increased at a dose of 50 mg/kgBW extract. Conclusions: There are differences in macrophages between the liver and lungs. Ethanol Extract of Decalobanthus mammosus (Lour.) A.R.Simões & Staples Tubers on lung macrophage cells, the frequency of macrophage activity is much higher compared to the liver.

Co-Authors A, Aulanniam Agoes Soegianto Agus Abadi Agus Heri Setyo Wahyudi Alifah Hayati Andita Ayu Mandasari Aulanni'am, Aulanni'am Bambang Poernomo Betti Lukiati Betti Lukiati Binti Yunaida Djoko Agus P. Dwi Winarni Eko Prihiyantoro Eko Prihiyantoro Endah Rochmatika Endang Dewi Masithah Evy Diah Woelansari farach Firli Rohmah Primula Dewi Harianto Notopuro Herdiansyah, Mochammad Aqilah I Gede Widhiantara I.B. Rai Pidada Inge Permatasari Intan Ayu Pratiwi Intan Permata Putri Intan Permata Putri Is Yuniar Juliana Christyaningsih Khambali Khambali Kris Cahyo Mulyatno Kusala, Muhammad Khaliim Jati Kusala Lailatul Lutfiyah, Lailatul Listijani Suhargo, Listijani Mammed Sagi Manikya Pramudya Mochammad Amin Alamsjah Mochammad Aqilah Herdiansyah Mufasirin Mutiarawati, Diah Titik Nadyatul Ilma Indah Savira Nanik Siti Aminah Natalia Tri Astuti Nunuk Dyah Retno Lastuti Nurmalasari Darsono Nurmalasari Darsono P Kumaladewi Panjaitan, Novaria Sari Dewi Panjaitan Permatasari, Anak Agung Ayu Putri Pieter Kakisina Pujiastuti Wahyuningsih , Sri Puspita Sari, Evi Putri Andreyna Saragi Putu Angga Wiradana Ramadhana Ramadhana Retnosari Apriasti Ria Harmonis Rifaid Nur Arsad Rohmah, Etik Ainun Rosalia Nuril Qolbi Rr. Juni Triastuti Samekto Wibowo Sapto Andriyono Sari, Ni Kadek Yunita Sari Siti Fatimatuz Zahra Siti Rahayu Sri Kadarsih Soedjono Sri Puji Astuti Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Sri Subekti Sugiharto Suhendra Agung Wibowo Sukardiman Susanto, Awaluddin Susilowati, Rina Priastini Suvia Widyaningrum Teguh Hari Sucipto, Teguh Hari Tri Dewanti W. Victorin Mega Putri Wiartini, Ni Wayan Ayu Wiartini Widhi Dyah Sawitri YULIA IRNIDAYANTI Yuni Kilawati Zubaidah Ya’cob

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