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All Journal HAYATI Journal of Biosciences Jurnal Pendidikan dan Pembelajaran Khatulistiwa (JPPK) Prosiding Seminar Biologi BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Biotechnology Majalah Obat Tradisional Jurnal Teknologi Laboratorium JMM (Jurnal Masyarakat Mandiri) Biosaintifika: Journal of Biology & Biology Education Jurnal Labora Medika Gorontalo Journal of Public Health Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Borneo Journal of Medical Laboratory Technology Narra J Quality : Jurnal Kesehatan Sureq: Jurnal Pengabdian Masyarakat Berbasis Seni dan Desain Prosiding University Research Colloquium Media Kesehatan Politeknik Kesehatan Makassar Jurnal Kefarmasian Indonesia
Sri Darmawati
Muhammadiyah Semarang University
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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PRODUCTION OPTIMIZATION, PARTIAL PURIFICATION, AND THROMBOLYTIC ACTIVITY EVALUATION OF PROTEASE OF Bacillus cereus HSFI-10 Ainutajriani, Ainutajriani; Darmawati, Sri; Zilda, Dewi Seswita; Afriansyah, Muhammad Ardi; Saptaningtyas, Ragil; Ethica, Stalis Norma
BIOTROPIA Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1765

Abstract

Cardiovascular disease is the primary cause of mortality in the world due to the formation of blood clots or thrombi in blood vessels. Bacterial proteases commonly function as thrombus dissolver agents in the pharmaceutical industry. Bacterial isolate HSFI-10 (Holothuria scabra Fermented Intestine-10) previously isolated from Rusip fermented sea cucumber had demonstrated thrombolytic activity. This study aimed to produce crude protease of HSFI-10 strain at an optimized incubation time and determine the thrombolytic activity of crude and dialysate proteases on A, B, AB, and O blood types. Isolate HSFI-10 was first molecularly identified and found to be Bacillus cereus with a homology level of 99.80% with Bacillus cereus strain ST06. The optimum crude enzyme was obtained after 48-h incubation with an activity of 222.52 U/mL, which increased to 438.84 U/mL after ammonium sulfate precipitation and dialysis. Clot lysis activity of crude enzymes was measured based on the gravimetry method on blood in the ABO system, showing results that ranged from 68.99% to 69.76%, while the dialysate ranged from 81.16% to 82.52%. In conclusion, partial purification of bacterial protease could increase both its specific and thrombolytic activities on human blood in the ABO system, with only 1% activity variability between A, B, AB, and O blood types.
EDUKASI BAHAYA TUBERKULOSIS PARU KEPADA MASYARAKAT RENTAN DALAM UPAYA ELIMINASI TB DI MALAYSIA Prastiyanto, Muhammad Evy; Iswara, Arya; Darmawati, Sri; Naqib, Ahmad
JMM (Jurnal Masyarakat Mandiri) Vol 8, No 6 (2024): Desember
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v8i6.26772

Abstract

Abstrak:.. Kegiatan edukasi mengenai bahaya tuberkulosis paru (TB) kepada masyarakat rentan di Malaysia bertujuan untuk meningkatkan kesadaran dan pemahaman tentang penyakit ini serta langkah-langkah pencegahannya. Dengan meningkatnya angka kasus TB di Malaysia, yang mencapai 26.781 kasus pada tahun 2022, termasuk 2.623 kematian, kegiatan ini menjadi sangat penting dalam upaya eliminasi TB. Melalui pendekatan partisipatif, edukasi dilakukan di komunitas dengan fokus pada kelompok berisiko tinggi, seperti individu dengan kondisi kesehatan yang mendasari dan mereka yang tinggal di lingkungan padat penduduk. Metode yang digunakan meliputi penyuluhan, distribusi materi informasi, dan penggunaan masker yang benar untuk memastikan pemahaman yang baik tentang gejala, cara penularan, dan pentingnya penggunaan masker serta kepatuhan terhadap pengobatan yang dilakukan di Pusat kecemerlangan perkhidmatan klinik UCMI Malaysia. Pasien rentan terinfeksi oleh bakteri penyebab TB, yang merupakan pasien tetap dengan usia senja pada Pusat kecemerlangan perkhidmatan klinik hemodialisis UCMI Malaysia yang berjumlah 51 pasien. Hasil dari kegiatan ini menunjukkan peningkatan signifikan dalam pengetahuan peserta mengenai TB dari yang tidak paham sebesar 75% menjadi hanya 10% dan perilaku pencegahan setelah mengikuti program edukasi. Diharapkan bahwa dengan meningkatkan kesadaran masyarakat, kita dapat berkontribusi pada pengurangan angka kejadian TB dan mendukung upaya pemerintah dalam mencapai eliminasi TB di Malaysia.Abstract: The education program on the dangers of pulmonary tuberculosis (TB) to vulnerable communities in Malaysia aims to increase awareness and understanding of the disease and preventive measures. With the increasing number of TB cases in Malaysia, reaching 26,781 cases by 2022, including 2,623 deaths, this activity is crucial in TB elimination efforts. Through a participatory approach, education is conducted in the community with a focus on high-risk groups, such as individuals with underlying health conditions and those living in densely populated neighborhoods. The methods used included counseling, distribution of information materials, and the correct use of masks to ensure a good understanding of the symptoms, modes of transmission, and importance of mask use as well as adherence to treatment conducted at the UCMI Malaysia clinical service excellence center. Patients susceptible to infection by TB-causing bacteria, who are permanent patients with old age at the Center for the brilliance of hemodialysis clinic services UCMI Malaysia with a total of 51 patients. The results of this activity showed a significant improvement in the participants' knowledge of TB from 75% to only 10% of non-understanding and preventive behaviors after attending the education program. It is hoped that by increasing public awareness, we can contribute to the reduction of TB incidence and support the government's efforts in achieving TB elimination in Malaysia.
Antibacterial Activity of Myrmecodia pendens (Ant Nest Plant) against Multidrug Resistance Bacteria from Clinical Samples Prastiyanto, Muhammad Evy; Ernanto, Aditya Rahman; Darmawati, Sri; Maharani, Endang Tri Wahyuni; Mulyanto, Arif; Rukmana, Rizal Maarif; Putri, Wahyu Aristyaning; Kusmita, Lia; Sah, Shiv Kumar; Geleta, Daniel
Majalah Obat Tradisional Vol 31, No 1 (2026)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.110761

Abstract

The emergence of multidrug-resistant (MDR) bacteria causes a substantial barrier to treatment, demanding the development of new antibacterial medicines. Myrmecodia pendens (ant nest plant), an indigenous medicinal species, is esteemed for its potential medicinal properties. This study aimed to examine the antibacterial efficacy of M. pendens extracts against MDR bacterial strains (Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii, and Pseudomonas aeruginosa) obtained from clinical samples. Extracts were prepared via maceration using ethanol, chloroform, and n-hexane solvents. Antibacterial efficacy was evaluated using the agar diffusion method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays. The ethanol solvent extract exhibited the most significant antibacterial activity, particularly against P. aeruginosa, with an inhibition zone of 23.15 ± 0.21 mm at 100 mg/mL. The MIC and MBC values were 3.13 mg/mL and 12.5 mg/mL, respectively. The chloroform and n-hexane extracts showed lower, but comparable, inhibitory effects against the tested strains. These findings provide compelling evidence that M. pendens extracts, especially the ethanol extracts, represent a promising source for developing alternative antibacterial agents to combat MDR infections.
PELATIHAN DASAR OLAH VOKAL SISWA SANGGAR SENI EX ART SMAN 3 PANGKEP Darmawati, Sri; Inayah, Faidhul; Afia, Selfa; Arisyi, Dani Fajrul; Hattah, Muh. Afif Novaldy
Sureq: Jurnal Pengabdian Masyarakat Berbasis Seni dan Desain Vol 5, No 1 (2026): Januari-Juni
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26858/srq.v5i1.84882

Abstract

Kemampuan vokal merupakan salah satu kompetensi esensial dalam seni pertunjukan, terutama pada kegiatan ekstrakurikuler sanggar seni di lingkungan sekolah, seperti halnya pada sanggar seni EX Art SMAN 3 Pangkep. Namun demikian, sebagian besar siswa belum menguasai teknik dasar olah vokal yang mencakup pernapasan, artikulasi, intonasi, resonansi, dan ekspresi dalam bernyanyi. Keterbatasan tersebut berdampak pada rendahnya kualitas performa seni serta menurunnya kepercayaan diri siswa dalam mempresentasikan karya seni, baik di lingkungan sekolah maupun masyarakat. Kegiatan pengabdian ini dilaksanakan melalui pendekatan pelatihan partisipatif yang mencakup penyampaian materi teoretis, demonstrasi teknik olah vokal, praktik terbimbing, serta evaluasi capaian kemampuan vokal siswa. Pelaksanaan kegiatan dirancang dalam empat tahapan, meliputi tahap persiapan, pelaksanaan pelatihan, pendampingan praktik, dan penilaian hasil latihan. Sasaran kegiatan ini adalah siswa anggota sanggar seni sekolah yang memiliki minat dan potensi di bidang seni musik dan vokal. Hasil kegiatan mengindikasikan adanya peningkatan yang signifikan terhadap pemahaman dan keterampilan siswa dalam mengaplikasikan teknik dasar olah vokal, meliputi pengelolaan pernapasan secara tepat, kejelasan pelafalan, serta kemampuan menjaga ketepatan nada dan tempo. Di samping itu, kegiatan pelatihan ini juga berkontribusi terhadap peningkatan kepercayaan diri, kemampuan kolaboratif, dan daya kreativitas siswa dalam konteks seni pertunjukan. Dengan demikian, pelatihan dasar olah vokal terbukti menjadi salah satu intervensi yang efektif dalam mendukung pengembangan kompetensi seni siswa sekaligus meningkatkan mutu kegiatan sanggar seni di sekolah.
Karakterisasi Imunogenesitas Protein Pilli Salmonella Typhi: Analisis Absorban Antibodi dan Spesifisitas dengan Western Blot pada Mencit Balb/C Hidayani, Al; Darmawati, Sri; Ramdhani , Fitria Hariati; Rahmah, Windya Nazmatur
Borneo Journal of Medical Laboratory Technology Vol. 8 No. 2 (2026): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v8i2.12477

Abstract

Protein pili berperan dalam adhesi dan bersifat imunogenik, berpotensi sebagai kandidat vaksin subunit. Penelitian ini menganalisis imunogenesitas protein pili S. typhi isolat BA07.4 melalui pengukuran absorbansi antibodi dan karakterisasi spesifisitasnya. Protein pili diisolasi dengan metode biphasic, dimurnikan, dan digunakan untuk imunisasi 30 ekor mencit Balb/C (6 kelompok: kontrol dan dosis 2, 3, 4, 5, 6 µg) secara intraperitoneal tiga kali. Kadar antibodi diukur dengan inhouse ELISA (λ 405 nm) dan spesifisitasnya dianalisis dengan Western blot. Rerata absorbansi tertinggi pada dosis 5 µg (2,827), diikuti dosis 6 µg (2,678), 4 µg (2,456), 3 µg (2,134), 2 µg (1,856), dan kontrol (0,423). Analisis One Way ANOVA menunjukkan perbedaan signifikan antar kelompok (p=0,000) dengan semua kelompok perlakuan berbeda signifikan terhadap kontrol (p<0,05). Western blot menunjukkan pita spesifik pada berat molekul 87, 58, dan 42 kDa pada kelompok dosis 5 µg, sementara kontrol tidak menunjukkan pita. Protein pili S. typhi bersifat imunogenik dengan dosis optimum 5 µg, menghasilkan antibodi yang secara spesifik mengenali sub unit protein pili 87, 58, dan 42 kDa, mendukung potensinya sebagai kandidat vaksin subunit.
Co-Authors A.A. Ketut Agung Cahyawan W Afia, selfa Afriansyah, M. Ardi Afriansyah, Muhammad Ardi Ainutajriani, Ainutajriani Al Ansyari, Mas’ud Uqbah Ana Hidayati Mukaromah Anak Agung Wiradewi Lestari Aprilia Indra Kartika, Aprilia Indra Arif Mulyanto, Arif Arisyi, Dani Fajrul Arya Iswara Batara, Murni Budi Santosa Budi Santosa BUDI SETIADI DARYONO Cahyaningrum, Dea Galuh Daryono, Budi S. Dewi Seswita Zilda Edward Kurnia Setiawan Limijadi, Edward Kurnia Setiawan Endang Tri Wahyuni Maharani Ernanto, Aditya Rahman Ethica, Stalis N. Faisal Faisal Fandhi Adi Wardoyo Fariza Yulia Kartika Sari Felim, Jethro Fuad, Hayatun Geleta, Daniel Hariutami, Desi Hattah, Muh. Afif Novaldy Hidayani, Al Hikmah, Annisa Nurul Inayah, Faidhul Jannah, Erni M Joharlinda, Joharlinda La’lang, Marselaonety LANGKAH SEMBIRING Lia Kusmita Listiyanti, Apriliya Manufandu, Anjar Richardo Mardiyah Hayati Masashi Kawaichi, Masashi Maulida, Gita Rossa NANIK RAHMANI Naqib, Ahmad Nasruddin, N Nur Hidayah Palimbongan, Junita Rensa Permana, Atna Prastiyanto, Muhammad E. Prastiyanto, Muhammad Evy Prastiyanto, Muhammad Evy Putri, Desty Ratna Putri, Wahyu Aristyaning Ragil Saptaningtyas, Ragil Ramdhani , Fitria Hariati Retnaningrum, Endah Rinaldi, Muhamad Rizqi Rizal Maarif Rukmana, Rizal Maarif Rosnita . Rustiyarso . Sah, Shiv Kumar Salsabila, Nia Sa’adah, Arina Lis Setianingsih, Erna Silvestre, Richard David Sri Sinto Dewi Stalis Norma Ethica Sukoharjanti, Bintari Tri Sulistyaningtyas, Ayu Rahmawati Susanti, A.Meryam Syaiful Anwar Trianes, Juwy Tulus Ariyadi Wayan T. Artama Wayan T. Artama Widya Asmara Wilson, Wildiani Windya Nazmatur Rahmah Zilda, Dewi S. Zuraida Zuraida