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All Journal HAYATI Journal of Biosciences Media Veteriner Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Journal of Education and Learning (EduLearn) Research Journal of Life Science AGRIVITA, Journal of Agricultural Science Journal of Mathematical and Fundamental Sciences Molecular and Cellular Biomedical Sciences (MCBS) Biosfer: Jurnal Pendidikan Biologi Biosaintifika: Journal of Biology & Biology Education Jurnal Eduscience (JES) Berkala Penelitian Hayati Journal of Global Pharma Technology Jurnal Kedokteran Hewan Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education)
Sutiman Bambang Sumitro
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Brawijaya University
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Social Sciences Veterinary Other

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Direct and Indirect Effect of TNFα and IFNγ Toward Apoptosis in Breast Cancer Cells Wahyu Widowati; Diana Krisanti Jasaputra; Sutiman Bambang Sumitro; Mochammad Aris Widodo; Ervi Afifah; Rizal Rizal; Dwi Davidson Rihibiha; Hanna Sari Widya Kusuma; Harry Murti; Indra Bachtiar; Ahmad Faried
Molecular and Cellular Biomedical Sciences Vol 2, No 2 (2018)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v2i2.21

Abstract

Background: Breast cancer (BC) is the leading cause of death cancer in women. Cancer therapies using TNFα and IFNγ have been recently developed by direct effects and activation of immune responses. This study was performed to evaluate the effects of TNFα and IFNγ directly, and TNFα and IFNγ secreted by Conditioned Medium-human Wharton’s Jelly Mesenchymal Stem Cells (CM-hWJMSCs) toward apoptosis of BC cells (MCF7).Materials and Methods: BC cells were induced by TNFα and IFNγ in 175 and 350ng/mL, respectively. CM-hWJMSCs were produced by co-culture hWJMSCs and NK cells that secreted TNFα, IFNγ, perforin (Prf1), granzyme B (GzmB) for treating BC cells. The BC cells were treated with CM-hWJMSCs in 50%. The expression of apoptotic genes Bax, p53, and the antiapoptotic gene Bcl-2 were determined using RT-PCR.Results: TNFα and IFNγ at concentration of 350 ng/mL induced higher Bax expression compared to 175 ng/mL. TNFα and IFNγ 350 ng/mL, 175 ng/mL induced p53 expression, whilst TNFα and IFNγ at 350 ng/mL decreased Bcl-2 expression. Perf1, GzmB, TNFα and IFNγ-containing CM-hWJMSCs induced significantly apoptosis percentage, induced Bax expression, but did not effect p53, Bcl-2 expression.Conclusion: TNFα and IFNγ directly induce Bax, p53, decrease Bcl-2 gene expression. The Prf1, GzmB, TNFα, IFNγ-containing CM-hWJMSCs induce apoptosis and Bax expression.Keywords: breast cancer, Wharton’s Jelly mesenchymal stem cells, TNFα, IFNγ
Anticancer mechanism of Pisangulin angulata through in silico and development as teaching material Agus Muji Santoso; Bayu Guruh Binangkit; Mohamad Amin; Sutiman Bambang Sumitro; Betty Lukiati; Mumun Nurmilawati; Poppy Rahmatika Primandiri; Ida Rahmawati; S Sulistiono; Yayoi Kodama
Biosfer: Jurnal Pendidikan Biologi Vol 12 No 1 (2019): Biosfer: Jurnal Pendidikan Biologi
Publisher : Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (366.066 KB) | DOI: 10.21009/biosferjpb.v12n1.45-57

Abstract

This study aims to reveal the anticancer mechanism of the bioactive compound of Pisangulin angulata and to assess the feasibility of that results as teaching material. This research includes descriptive explorations. In the first stage, an in silico analysis was performed by molecular docking method between physalin compounds and GLI1 protein. The second steps of this study aim to develop teaching materials based research using the Analysis, Reorganizing, Piloting Class, and Evaluating (ARPE models). Feasibility test was carried out by experts and practitioners. Think Pair Share was used in the pilot project. Student motivation and misconception were recorded using SMI and CRI instrument. This study reveals that physalin B has higher activities than controls. The type of chemical bond that is formed between GLI1 amino acids residues with physalin is hydrogen bonds and hydrophobic bonds. The visualization of the types of bonds in that molecular docking between GLI1 amino acid residues and physalin has a high degree of feasibility (89) and can be used to enrich Chemistry for Biology lectures. The visualization of these chemical bonds can increase learning motivation and can improve the understanding of the concept of chemical bonds.
Bovine And Human Zona Pellucida 3 Gene Glycans Site Prediction Using In Silico Analysis Nurul Jadid Mubarakati; Aulanni'am Aulanni'am; Sutiman Bambang Sumitro; Gatot Ciptadi
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.04.03.08

Abstract

Zona pellucida is one of the protective layer of the egg cell and has a function as an intermediary species-specific fertilization. Glycoproteins of human and bovine zona pellucida is composed of three types, namely ZP1, ZP2 and ZP3. ZP3 gene has amino acid sequence homology with other mammals. Oligosaccharides components of the zona pellucida glycans are composed from units of asparagine residues (N -linked) and serine/threonine (O -linked). The aims of this study was to analyze the DNA sequences of human and bovine and further predicts glycans site on amino acid sequence of human and bovine ZP3. In this study, ZP3 gene fragments have been isolated from bovine and humans were analyzed in Silico. This work were conducted by comparing the data of DNA sequence from human and bovine PCR product using NCBI BLAST. The results showed that there were similarities at amino acid positions number 23-38. bZP3 sequence had three glycans site (Asn-X-Thr/Ser) and one site on hZP3 glycans. One of the sites was conserved between the two species.
The Alteration of SNAIL2 and MMP-9 Protein Expression of JEG-3 Choriocarcinoma Cell by Methanolic Extract of Tamarindus Indica Fruit Candra Rini Hasanah Putri; Sutiman Bambang Sumitro; Setyawati Soeharto; Karyono MIntaroem
Journal of Global Pharma Technology Volume 11 Issue 04.
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.953 KB)

Abstract

Objective: Tamarindus indica is one of the famous natural plant that has various effects on health. Recent research has begun to examine the effect of Tamarindus indica on cell malignancy. One of the things that happens in cell malignancy is an increasing ability of cell migration, which is characterized by increasing expression of Snail2 and MMP-9. This study was conducted to determine the effect of extract of Tamarindus indica Fruit Extract (TIFE) towards Snail2 and MMP-9 expression in JEG-3 cells.Methods :.TIFE were extracted by using methanol. JEG-3 cells were grown in culture medium treated with TIFE (at 0, 25, 50 and 100 µg/ml). Immunofluorescence staining was carried out, and observed under Confocal Laser Scanning Microscope.Result: Snail2 expression after treatment with TIFE 50 and 100 µg/ml higher than control- (P=0.015 ; P=0.001) whereas at MMP-9 expression there was a biphasic pattern, in which the MMP-9 expression peaked at concentration of 50 µg/ml (P=0.002), then MMP-9 expression decreased at 100 µg/ml concentration. Conclusion : The expression of Snail2 influence MMP-2 expression after treat with TIFE, so it might increase the potential for JEG-3 cell migration Keywords : Methanolic extract, Tamarindus indica, Snail2, MMP-9
Isolasi dan Karakterisasi Protein 100kDa dari Membran Kepala Spermatozoa Kambing Bayyinatul Muchtaromah; Sutiman Bambang Sumitro; Soemarno Soemarno; Trinil Susilawati
The Journal of Experimental Life Science Vol. 2 No. 1 (2012)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1053.644 KB) | DOI: 10.21776/ub.jels.2012.002.01.03

Abstract

Antigen adalah protein yang dapat menginduksi terbentuknya antibodi. Berat molekul protein sebesar 100 kDa dapat bertindak sebagai imunogen yang kuat. Tujuan dari penelitian ini adalah untuk mencari kandidat vaksin imunokontrasepsi dan melakukan isolasi serta karakterisasi protein 100 kDa yang diduga mempunyai peranan penting di dalam interaksi spermatozoa-ovum. Penelitian ini merupakan penelitian eksploratif. Materi Protein 100 kDa diisolasi dari membran spermatozoa kambing menggunakan detergen N-Octyl-glycopiranoside kemudian dirunning menggunakan metode SDS-PAGE dan dikoleksi dengan elektroelusi. Karakterisasi Protein 100 kDa meliputi penentuan berat molekul melalui SDS-PAGE, penentuan titik isoelektrik (pI) dengan IEF (isoelectric focusing) dan kandungan protein dengan metode Biuret. Hasil running isolat protein membran spermatozoa kambing menggunakan metode SDS-PAGE diperoleh pita protein yang terdiri atas 7 pita dengan berat molekul 166,7; 122; 100; 82,8;  60,4; 29,9 dan 14,8 kDa. Isolat Protein 100 kDa diperoleh dengan purifikasi menggunakan metode elektroelusi. Hasil IEF gel elektroforesis Protein 100 kDa dari membran spermatozoa kambing yang diejakulasikan ini mempunyai tiga titik isoelektrik yaitu 6,37; 6,05 dan 5,64. Kandungan protein dalam isolat Protein 100 kDa sebesar 2375 ± 5,65 µg/mL. Hasil ini menunjukkan bahwa isolat protein 100 kDa dapat digunakan sebagai imunogen. Kata Kunci : isolasi, karakterisasi, membrane spermatozoa, protein 100kDa
Analisis Titer Antibodi Bovine Zona Pellusida 3 (Anti-bZP3) Hasil Induksi Bovine Zona Pellusida 3 (bZP3) pada Kera (Macaca fascicularis) Maris Kurniawati; Sutiman Bambang Sumitro; Aulanni'am Aulanni'am
The Journal of Experimental Life Science Vol. 2 No. 1 (2012)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1078.063 KB) | DOI: 10.21776/ub.jels.2012.002.01.08

Abstract

Zona pellusida 3 merupakan molekul glikoprotein yang berfungsi sebagai reseptor primer spermatozoa dalam proses fertilisasi. Bovine zona pellusida 3 (bZP3) dapat dikembangkan sebagai target antigen untuk vaksin imunokontrasepsi. Efektivitas kerja antibodi sangat dipengaruhi oleh titer antibodi. Tujuan dilakukan  penelitian ini adalah mengetahui profil titer antibodi bovine zona pellusida 3 (anti-bZP3) hasil induksi bovine zona pellusida 3 (bZP3) pada kera (Macaca fascicularis). Imunisasi bZP3 pada kera (Macaca fascicularis) dilakukan secara sub kutan (SC) menggunakan CFA (Complete Freund's Adjuvant) untuk imunisasi pertama dan IFA (Incomplete Freund's Adjuvant) untuk imunisasi booster 1 dan booster 2. Serum dipanen sebanyak 4 kali setelah booster 1 dan 2. Hasil analisa menunjukkan berat molekul anti-bZP3 dari kera adalah 160 kDa. Titer naik mulai minggu ketiga hingga kelima dan turun lagi setelah minggu keenam setelah imunisasi pertama (minggu pertama hingga keempat pasca booster pertama) dan titer naik kembali pada minggu ke tujuh hingga kesembilan serta turun pada minggu ke sepuluh setelah imunisasi pertama (minggu pertama hingga ke empat pasca booster kedua). Titer antibodi tertinggi dicapai pada minggu kesembilan pasca imunisasi pertama atau minggu ketiga pasca booster kedua. Kata kunci: bZP3, imunokontrasepsi, Macaca fascicularis, zona pellusida  
Effect of Ethylene Glycol Concentration and Length of Exposure on In Vitro Fertility of Bovine Oocyte Sri Wahjuningsih; Suhartojo Hardjopranjoto; Sutiman Bambang Sumitro
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.813 KB) | DOI: 10.21157/j.ked.hewan.v4i2.3097

Abstract

The purpose of the study was to determine the influence of the concentration of ethylene glycol (EG) and length of exposure to levels of bovine oocytes in vitro fertility. This research was conducted using a completely randomized design factorial 5x3 with 7 replicates. The first factor was the concentration of cryoprotectants EG 10, 20, 30, 40, and 50%. The second factor was the length of exposure 1, 3, and 5 minutes. The results showed that the concentration of EG and the length of exposure were effect on fertilized oocytes (P0.05). The level of oocytes in vitro fertility after vitrification in 30% EG and long exposure to 3 minutes did not different (P0.05) compared to fresh oocytes, while the EG treatment 10, 20, 40, and 50% significantly showed a lower fertility rate than the 30% EG (P0.05). It was concluded that the concentration of EG and length of exposure were effect on fertilized oocytes. The highest oocytes in vitro fertility level were found in 30% EG concentration with 3 minutes exposure time.
PENGARUH INDUKSI EPIDERMAL GROWTH FACTOR (EGF) TERHADAP PROTEIN Cx43 SELAMA EKSPANSI SEL KUMULUS Chomsa Dintasari Umi Baszary; Sutiman Bambang Sumitro; Mochammad Sasmito Djati; Edi Widjajanto
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (562.406 KB) | DOI: 10.21157/j.ked.hewan.v6i1.350

Abstract

Tujuan penelitian ini untuk mengetahui pengaruh induksi EGF terhadap lokalisasi Cx43 dan gap junction selama ekspansi sel kumulus. Hasil penelitian ini menunjukkan bahwa ekspansi sel kumulus mempunyai signifikansi dengan meningkatnya konsentrasi EGF dan waktu kultur. Ekspansi sel kumulus diindikasikan dengan perubahan bentuk sel menjadi memanjang dan menyebar. Ekspresi Cx43 meningkat pada kultur jam ke-5 dan semakin meningkat pada kultur jam ke-10 dengan semakin padatnya protein pada badan sel. Pada kultur jam ke-15 terjadi penurunan ekspresi Cx43 pada badan sel. EGF berpengaruh terhadap peningkatan ekspansi sel kumulus tetapi tidak pada ekspresi Cx43 dengan bertambahnya konsentrasi dan waktu kultur. Hal ini menunjukkan bahwa komunikasi antar sel kumulus menurun, karena jarak antar sel yang semakin jauh.
Analysis of the Relationship Between Concept Mastery and Problem-Solving Skills of Pre-Service Biology Teachers in Human Physiology Courses Finga Fitri Amanda*; Sutiman Bambang Sumitro; Sri Rahayu Lestari*; Ibrohim Ibrohim
Jurnal Pendidikan Sains Indonesia Vol 9, No 3 (2021): JULY 2021
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.243 KB) | DOI: 10.24815/jpsi.v9i3.19956

Abstract

21st century learning focuses on problem-solving skills. A higher level of education requires an understanding of a more systematic concept to elaborate problems. Problem-solving skill is one of the fundamental cognitive processes. It involves higher order thinking to discover solutions or ideas. This research aims to analyze the relationship between concept mastery and problem-solving skill of pre-service biology teacher in human physiology courses. This is descriptive correlational research conducted in Sulthan Thaha Saifuddin State Islamic University Jambi. The sample was selected using the purposive sampling technique. Respondents in this study were 33 students in semester VI. The instrument used in the study was an essay test of concept mastery integrated with problem-solving. The results showed that the average scores of the students' concept mastery and problem-solving were 56.8 and 58.0, respectively. Both included in the poor category. It also showed that there was a relationship between students' concept mastery and problem-solving skill. The correlation value was 0.76, with a significance value of p0.05; therefore, it included in the strong category.
ANALYSIS OF CRITICAL THINKING SKILLS FOR BIOLOGY TEACHER PROSPECTIVE AT UNIVERSITY OF PAPUA Iwan Iwan; Sutiman Bambang Sumitro; Ibrohim Ibrohim; Fatchur Rohman
JURNAL EDUSCIENCE Vol 10, No 2 (2023): Jurnal Eduscience (JES), (Authors from Turkey, Malaysia, Algeria, Indonesia)
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jes.v10i2.4516

Abstract

This research is a type of descriptive research with the aim of knowing the critical thinking ability profile of prospective biology teachers at the University of Papua. The population in this study were all prospective biology teacher students at the University of Papua. The sampling technique is purposive sampling, namely sampling based on a specific purpose. The number of respondents was 42 prospective biology teachers. The instrument is in the form of essay questions. Based on research data analysis, the average proportion of critical thinking skills for biology teacher candidates is 55.03. Furthermore, the proportions based on critical thinking criteria are 10% uncritical, 53.33% less critical, 36.67% critical and 2.38% very critical. This in general it can be interpreted that the critical thinking skills of prospective biology teachers at the University of Papua are in the less critical category, this is very important to be empowered as a provision to face competition in the era of the industrial revolution 4.0.Keywords: Analysis, Critical Thinking Skills, Prospective Biology TeachersAbstrak Penelitian ini merupakan jenis penelitian deskriptif dengan tujuan untuk mengetahui profil kemampuan berpikir kritis calon guru biologi di Universitas Papua. Populasi dalam penelitian ini adalah seluruh mahasiswa calon guru biologi di Universitas Papua. Teknik pengambilan sampel dengan cara Purposive Sampling yakni pengambilan sampel berdasarkan tujuan tertentu. Jumlah responden sebanyak 42 calon guru biologi. Instrumen berupa soal essay. Berdasarkan  analisis data hasil penelitian  rata-rata persentase  keterampilan berpikir kritis calon guru biologi yaitu 55,03. Selanjutnya persentase berdasarkan kriteria berpikir kritis yaitu  kategori tidak kritis 10%, kurang kritis 53,33%, kritis 36,67 % dan sangat kritis 2,38%. Dengan demikian secara umum dapat disimpulkan bahwa keterampilan berpikir kritis calon guru biologi Universitas Papua berada pada kategori kurang kritis, hal ini kiranya sangat penting  untuk diberdayakan sebagai bekal untuk menghadapi persaingan di era revolusi industry 4.0.Kata Kunci:  Analisis, Keterampilan Berpikir Kritis, Calon Guru Biologi
Co-Authors . AULANI’AM Agus Muji Santoso Ahmad Faried Annisa Amalia Arief Boediono Aucky Hinting Aulanni'am Aulanni'am Aulanni'am Aulanni'am Aulanni’am Aulanni’am Bambang Tri Rahardjo Bambang Tri Rahardjo Basuki B. Purnomo Bayu Guruh Binangkit Bayyinatul Muchtaromah Betty Lukiati Candra Rini Hasanah Putri Chomsa Dintasari Umi Baszary Diana Krisanti Jasaputra Dody Novrial, Dody Dwi Davidson Rihibiha Dwi Davidson Rihibiha Edi Widjajanto Ervi Afifah Ervi Afifah Fatchur Rohman Finga Fitri Amanda Finga Fitri Amanda* Gato Gato Gatot Ciptadi Hanna Sari Widya Kusuma Hanna Sari Widya Kusuma Harry Murti Harry Murti Ibrohim Ida Rahmawati Ignatius Riwanto Indra Bachtiar Indra Bachtiar Indra Wijaya Irvan Sidik Iwan, Iwan Karyono Mintaroem Mahanani Tri Asri Mahanani Tri Asri Maris Kurniawati Moch Sasmito Djati Mochammad Aris Widodo Mochammad Aris Widodo Mohamad Amin Mohammad Mabrur Mumun Nurmilawati Nia Kurniawan Nurul Jadid Mubarokati Philips Onggowidjaja Poppy Rahmatika Primandiri Rizal Rizal Rizal Rizal Setyawati Karyono Setyawati Soeharto Siti Imama Khoiriyah Siti Rasminah Chaelani soehartojo Hardjopranjoto Soemarno Soemarno Sri Rahayu Lestari Sri Wahjuningsih Sri Widyarti Suhartojo Hardjopranjoto Sulistiono TRINIL SUSILOWATI Umi Lestari Wahyu Widowati Wahyu Widowati Widjiati w Yayoi Kodama