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All Journal HAYATI Journal of Biosciences Media Veteriner Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Journal of Education and Learning (EduLearn) Research Journal of Life Science AGRIVITA, Journal of Agricultural Science Journal of Mathematical and Fundamental Sciences Molecular and Cellular Biomedical Sciences (MCBS) Biosfer: Jurnal Pendidikan Biologi Biosaintifika: Journal of Biology & Biology Education Jurnal Eduscience (JES) Berkala Penelitian Hayati Journal of Global Pharma Technology Jurnal Kedokteran Hewan Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education)
Sutiman Bambang Sumitro
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Brawijaya University
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Social Sciences Veterinary Other

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Cigarette Smoke Induces Colorectal Carcinogenesis in Wistar Rats by Decreasing The Expression of APC, MSH2 and MLH1 Dody Novrial; Ignatius Riwanto; Sutiman Bambang Sumitro; Indra Wijaya
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.8439

Abstract

Colorectal carcinogenesis induced by cigarette smoke requires at least 30-40 years. This long time duration causes an animal research conducted becomes relevant. This research was carried out to observe colorectal carcinogenesis due to cigarette smoke exposure in Wistar Rat. The observations focused on changes in epithelial morphology and expression of APC, MSH2 and MLH1. Twenty male Wistar rats inbreed strain were randomly allocated into control group and experimental group exposure to cigarettes smoke for 14 weeks and 28 weeks sequentially.  Colorectal epithelial morphology was assessed on the histopathology examination, whereas the expression of APC, MSH2 and MLH1 was assessed on aspect of immunohistochemistry. The comparative analysis between the two groups was performed using non-parametric Mann-Whitney U test. Histology of colorectal epithelium showed pattern of colitis associated cancer that was significant both in 14 weeks and 28 weeks of treatment.  This research indicated negative expression of APC, MSH2 and MLH1 in the colorectal cancer that were significant at 28 weeks of exposure. This research implies that chronic exposure to cigarette smoke can induce colitis associated colorectal cancer through decreased expression of APC, MSH2 and MLH1. 
THE EFFECT OF PBL INTEGRATED WITH IGYA SER HANJOB'S LOCAL WISDOM TO IMPROVE STUDENTS' PROBLEM SOLVING SKILLS IN CONSERVATION BIOLOGY COURSES Iwan Iwan; Sutiman Bambang Sumitro; Ibrohim Ibrohim; Fatchur Rohman
JURNAL EDUSCIENCE Vol 11, No 1 (2024): Jurnal Eduscience (JES), (Authors from Nigeria, India, and Indonesia)
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jes.v11i1.5537

Abstract

This research aims to determine the effect of PBL integrated with Igya Ser Hanjob's local wisdom on students' problem solving skills in the Conservation Biology course. The sample for this research was Biology Education Semester VII students taking Conservation Biology courses, totaling 48 students, namely class A (experimental class) with 24 students and class B (control class) with 24 students. This type of research is quasi-experimental research using a nonequivalent control group design. The instruments used in this research were description tests and questionnaires. The data obtained were analyzed using descriptive and inferential statistics which had previously met the requirements for normality and homogeneity, then the Anacova test was carried out using the SPSS IMB 20 program. The results of the research showed 1) there was an increase in the average score of students' problem solving skills after implementing PBL containing local wisdom 2 ) there is a difference in student problem solving skills between the group of students who took part in PBL containing Igya ser hanjob local wisdom and the group of students who took part in conventional learning as evidenced by a significance value of 0.000 < 0.05. Therefore, it can be concluded that PBL learning integrated with Igya Ser Hanjob's local wisdom can improve students' problem solving skills.Keywords: PBL, Problem solving skills, Igya ser hanjop, Local wisdom, Biology Conservation
MECHANISM OF INFECTION Spodoptera litura Nucleopolyhedrosis Multiple Virus (SpltMNPV) ON MIDGUT EPITHELIAL CELL ARMY WORM (Spodoptera litura) Observed by TEM Mahanani Tri Asri; Siti Rasminah Chaelani; Bambang Tri Rahardjo; Sutiman Bambang Sumitro
AGRIVITA Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i1.115

Abstract

Spodoptera litura is one of  agricultural crop pests. They are resistant to chemical insecticides. One of arternate biological control is Spodoptera litura Nucleopolyhedrosis Multiple Virus (SpltMNPV) Penelitian ini dilakukan untuk mengetahui bagaimana mekanisme SpLtMNPV dalam menginfeksi sel epithel midgut larva S. The research was conducted to determine how SpltMNPV infected midgut epithelial cells of S. litura secara in vitro . litura larvae in vitro.  The mechanism of infection was observed by a transmission electron microscopy (TEM).  The result of this observation showed that infecting midgut cell by  SpltMNPV involved 5 phases, they are, 1) the attachment of  SpltMNPV at the membrane of suitable host, 2) the penetration, formation of channels and release of protein envelope, 3) the biosynthesis of virus components in the cell nucleus, 4) the assembling of virus components, and 5) the releasing of MNPV/multiplenucleocapsid through budding. Keywords: mechanisms of infection, Spodoptera litura multiple nucleopolyhedrosis virus, midgut army worm larvae cells.
Co-Authors . AULANI’AM Agus Muji Santoso Ahmad Faried Annisa Amalia Arief Boediono Aucky Hinting Aulanni'am Aulanni'am Aulanni'am Aulanni'am Aulanni’am Aulanni’am Bambang Tri Rahardjo Bambang Tri Rahardjo Basuki B. Purnomo Bayu Guruh Binangkit Bayyinatul Muchtaromah Betty Lukiati Candra Rini Hasanah Putri Chomsa Dintasari Umi Baszary Diana Krisanti Jasaputra Dody Novrial, Dody Dwi Davidson Rihibiha Dwi Davidson Rihibiha Edi Widjajanto Ervi Afifah Ervi Afifah Fatchur Rohman Finga Fitri Amanda Finga Fitri Amanda* Gato Gato Gatot Ciptadi Hanna Sari Widya Kusuma Hanna Sari Widya Kusuma Harry Murti Harry Murti Ibrohim Ida Rahmawati Ignatius Riwanto Indra Bachtiar Indra Bachtiar Indra Wijaya Irvan Sidik Iwan, Iwan Karyono Mintaroem Mahanani Tri Asri Mahanani Tri Asri Maris Kurniawati Moch Sasmito Djati Mochammad Aris Widodo Mochammad Aris Widodo Mohamad Amin Mohammad Mabrur Mumun Nurmilawati Nia Kurniawan Nurul Jadid Mubarokati Philips Onggowidjaja Poppy Rahmatika Primandiri Rizal Rizal Rizal Rizal Setyawati Karyono Setyawati Soeharto Siti Imama Khoiriyah Siti Rasminah Chaelani soehartojo Hardjopranjoto Soemarno Soemarno Sri Rahayu Lestari Sri Wahjuningsih Sri Widyarti Suhartojo Hardjopranjoto Sulistiono TRINIL SUSILOWATI Umi Lestari Wahyu Widowati Wahyu Widowati Widjiati w Yayoi Kodama