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All Journal HAYATI Journal of Biosciences Media Veteriner Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of Education and Learning (EduLearn) Research Journal of Life Science AGRIVITA, Journal of Agricultural Science Journal of Mathematical and Fundamental Sciences Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosfer: Jurnal Pendidikan Biologi Biosaintifika: Journal of Biology & Biology Education Jurnal Eduscience (JES) Berkala Penelitian Hayati Journal of Global Pharma Technology Jurnal Kedokteran Hewan Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education)
Sutiman Bambang Sumitro
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Brawijaya University
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Social Sciences Veterinary Other

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MECHANISM OF INFECTION Spodoptera litura Nucleopolyhedrosis Multiple Virus (SpltMNPV) ON MIDGUT EPITHELIAL CELL ARMY WORM (Spodoptera litura) Observed by TEM Mahanani Tri Asri; Siti Rasminah Chaelani; Bambang Tri Rahardjo; Sutiman Bambang Sumitro
AGRIVITA Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i1.115

Abstract

Spodoptera litura is one of  agricultural crop pests. They are resistant to chemical insecticides. One of arternate biological control is Spodoptera litura Nucleopolyhedrosis Multiple Virus (SpltMNPV) Penelitian ini dilakukan untuk mengetahui bagaimana mekanisme SpLtMNPV dalam menginfeksi sel epithel midgut larva S. The research was conducted to determine how SpltMNPV infected midgut epithelial cells of S. litura secara in vitro . litura larvae in vitro.  The mechanism of infection was observed by a transmission electron microscopy (TEM).  The result of this observation showed that infecting midgut cell by  SpltMNPV involved 5 phases, they are, 1) the attachment of  SpltMNPV at the membrane of suitable host, 2) the penetration, formation of channels and release of protein envelope, 3) the biosynthesis of virus components in the cell nucleus, 4) the assembling of virus components, and 5) the releasing of MNPV/multiplenucleocapsid through budding. Keywords: mechanisms of infection, Spodoptera litura multiple nucleopolyhedrosis virus, midgut army worm larvae cells.
The Effectiveness of the Problem-Based Learning Model to Enhance Students' Critical Thinking Skills on Conservation Biology Courses Iwan; Sutiman Bambang Sumitro; Ibrohim; Fatchur Rohman
Jurnal Penelitian Pendidikan IPA Vol 10 No 8 (2024): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i8.6959

Abstract

The purpose of this study is to ascertain how the problem-based learning approach affects students' critical thinking abilities in courses on conservation biology. This study design uses a non-equivalent control group in a quasi-experiment. Essay exam questions totaling 10 valid and reliable numbers served as the study's instrument. Pre-test and post-test questions were used to gather data. Descriptive statistics and inferential statistical tests, namely the covariance (ANCOVA) analysis with pretest values as variables at a significance level of 0.05%, were used to evaluate the research data. The IMB 23 SPSS program was utilized for data analysis because the data were homogeneous and regularly distributed. The findings demonstrated that, with a significance value of 0.000 < 0.05, there was a significant difference in the critical thinking abilities of students taught using the conventional model and those taught using the problem-based learning model. The conclusion is that in conservation biology classes, the problem-based learning approach helps students develop their critical thinking skills.
Oxyhydrogen Nanobubbles Suppress FoxP3 and Ki-67 Expression in a Wistar Rat Model of Hepatocellular Carcinoma Natasya Adiba Zahrah; Sri Widyarti; Dyah Kinasih Wuragil; Wibi Riawan; Olly Indrajani; Ahmad Lubab; Aditya Tri Hernowo; Sutiman Bambang Sumitro; Aulanni'am
Jurnal Penelitian Pendidikan IPA Vol 12 No 1 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i1.13858

Abstract

HCC is difficult to treat due to its complex tumor microenvironment and the ineffectiveness of available therapies. Gas-based anticancer approaches are promising, but are hampered by instability and difficulties in targeted delivery. HHOnbs (containing hydrogen (H2), oxygen (O2), and low-dose hydrogen peroxide (H2O2) at the nanoscale) have been proposed to improve the stability and precision of gas-based therapies. This study aimed to investigate the effects of HHOnbs on the immunoregulatory marker FoxP3 and the proliferation marker Ki-67 in a Wistar rat model of HCC induced by diethylnitrosamine (DEN)/carbon tetrachloride (CCl4). The design used was a true experimental design. HHOnbs were administered intravenously. Liver tissue was assessed using immunohistochemistry to measure FoxP3 and Ki-67 expression. HHOnbs treatment significantly reduced FoxP3 and Ki-67 expression (p<0.05). FoxP3 is a marker of regulatory T cells (Tregs), which often inhibit antitumor immune responses. Its decrease indicates an increased immune response potential. Ki-67 is a marker of cell proliferation. Its decrease indicates the suppression of cancer cell growth. Further investigations are warranted to compare its efficacy with standard therapies, assess different treatment durations, and elucidate the underlying molecular signaling pathways.
Co-Authors . AULANI’AM Aditya Tri Hernowo Agus Muji Santoso Ahmad Faried Ahmad Lubab Annisa Amalia Arief Boediono Aucky Hinting Aulanni'am Aulanni'am Aulanni'am Aulanni’am Aulanni’am Bambang Tri Rahardjo Bambang Tri Rahardjo Basuki B. Purnomo Bayu Guruh Binangkit Betty Lukiati Candra Rini Hasanah Putri Chomsa Dintasari Umi Baszary Diana Krisanti Jasaputra Dody Novrial, Dody Dwi Davidson Rihibiha Dwi Davidson Rihibiha Dyah Kinasih Wuragil Edi Widjajanto Ervi Afifah Ervi Afifah Fatchur Rohman Finga Fitri Amanda Finga Fitri Amanda* Gato Gato Gatot Ciptadi Hanna Sari Widya Kusuma Hanna Sari Widya Kusuma Harry Murti Harry Murti Ibrohim Ida Rahmawati Ignatius Riwanto Indra Bachtiar Indra Bachtiar Indra Wijaya Irvan Sidik Iwan Iwan, Iwan Karyono Mintaroem Mahanani Tri Asri Mahanani Tri Asri Moch Sasmito Djati Mochammad Aris Widodo Mochammad Aris Widodo Mohamad Amin Mohammad Mabrur Mumun Nurmilawati Natasya Adiba Zahrah Nia Kurniawan Nurul Jadid Mubarokati Olly Indrajani Philips Onggowidjaja Poppy Rahmatika Primandiri Rizal Rizal Rizal Rizal Setyawati Karyono Setyawati Soeharto Siti Imama Khoiriyah Siti Rasminah Chaelani soehartojo Hardjopranjoto Sri Rahayu Lestari Sri Wahjuningsih Sri Widyarti Suhartojo Hardjopranjoto Sulistiono TRINIL SUSILOWATI Umi Lestari Wahyu Widowati Wahyu Widowati Wibi Riawan Widjiati Widjiati, Widjiati Yayoi Kodama