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All Journal HAYATI Journal of Biosciences Jurnal Pendidikan Matematika dan IPA Prosiding Seminar Biologi AGRIVITA, Journal of Agricultural Science Sawwa: Jurnal Studi Gender ANNALES BOGORIENSES AGROTEK: Jurnal Ilmiah Ilmu Pertanian Jurnal Sains dan Teknologi Pertanian Proceeding Biology Education Conference Makara Journal of Health Research Jurnal Ekonomi, Manajemen, Akuntansi Jurnal El-Thawalib Annales Bogorienses Journal of Islamic Banking, Economics and finances (JIBEF)
Desriani Desriani
Research Centre for Biotechnology, LIPI. Jl. Raya Bogor Km 46, Kec. Cibinong, Kab. Bogor, 16911. Indonesia. Telp: +62-21-8754587.
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences

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Heterologous Expression of Recombinant Human Insulin Glargine (hIG) in Methylotrophic Yeast Pichia pastoris Herawati, Neng; Rubiyana, Yana; Desriani, Desriani; Santoso, Adi
Annales Bogorienses Vol. 26 No. 1 (2022): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2022.v26.n1.13-20

Abstract

World health organization (WHO) announced that diabetic patients increased significantly yearly worldwide. Consequently, the need for insulin becomes very large. Pichia pastoris, defined as methylotrophic yeast and a well-known expression and protein production host, is widely used for biopharmaceutical-based drug production. This research aims to synthesize human insulin glargine (hIG) protein in yeast P. pastoris. Human insulin glargine is a group of long-acting analogue insulin.We used a synthetic hIG-encoding gene constructed in frame with the truncated α-factor secretion signal in a pD902 expression vector. Recombinant plasmid pD902-hIG was linearized using Sac1 enzyme and transformed into P. pastoris genome. Multicopy clones were selected on YPDS plates containing Zeocin™ with concentrations of 100; 500; 1,000; and 2,000 µg//mL. Analyses using SDS-PAGE, slot blot, and Western blot showed that recombinant hIG protein had been obtained with a molecular weight of approximately 6,000 Daltons.
A Preliminary Report on The Syntheses of Oligonucleotide Primers in The National Research and Innovation Agency (NRIA) Atikana, Akhirta; Prasetyoputri, Anggia; Rubiyana, Yana; Herawati, Neng; Desriani, Desriani; Pratiwi, Riyona Desvy; Wulandari, Dwi; Sukmarini, Linda; Kusharyoto, Wien; Santoso, Adi; Putra, Masteria Yunovilsa; Lisdiyanti, Puspita
Annales Bogorienses Vol. 26 No. 1 (2022): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2022.v26.n1.21-27

Abstract

A PolyGen DNA Synthesizer is equipment that is used for synthesizing oligonucleotide primers for any amplification targets. Oligonucleotide primers are indispensable components for any Polymerase Chain Reaction (PCR)-based detections. In the present study a number of oligonucleotide primer sets were synthesized to target (1) the Human Insuline Glargin (HIG) and (2) the Human Erythropoietin (EPO), as well as (3) the RNA-dependent RNA Polymerase (RdRp) and (4) the Nucleocapsid (N) genes of the severe acute respiratory syndrome virus 2 (SARS-CoV-2). A solid-phase oligonucleotide synthesis method was used according to the default protocol of the Polygen’s instrument to synthesize primers at a 40 nmol scale. The synthesized primers in this study were compared to commercially produced primers in their ability to amplify the gene target(s) in PCR and quantitative real-time PCR (qPCR) reactions. The first two sets of primers showed similar results in PCR compared to commercial primers; however, these primers were not tested for qPCR due to sample limitation. In contrast, the primer sets 3 and 4 were not able to produce amplicons in PCR reactions and only the primer set 4 successfully amplified the gene target in qPCR. These results indicate that the crude primers synthesized in this study are promising candidates for molecular detection and diagnostics, but these primers would benefit from further optimization for routine applications.
Analisis Strategi Pemasaran Untuk Meningkatkan Volume Penjualan Pada Toko Emas Anak Rokan Di Kecamatan Kubu Babussalam Kabupaten Rokan Hilir Dalam Ekonomi Islam Zulfikar, Zulfikar; Desriani, Desriani; Wahyudi, Denny
Journal of Islamic Banking, Economics and Finance Vol. 1 No. 1 (2024): October 2024
Publisher : EL-EMIR Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63477/jibef.v1i1.79

Abstract

This research was conducted to see the increase in gold sales at Anak Rokan gold shops in Kubu Babussalam Rokan Hilir District and what strategies are used to increase sales volume and what, from an Islamic perspective, are the marketing strategies used at Anak Rokan gold shops. The research method used in this research is a qualitative method by conducting interviews with customers or buyers who shop at the Emas Anak Rokan store. Based on the results of the IE matrix analysis, Anak Rokan Gold Shop occupies Cell V, which means that the company or Gold Shop tends to carry out market penetration strategies and product development strategies. Based on the results of the Grand Strategy Matrix analysis, the Anak Rokan Gold Shop is located in quadrant 1, because according to the EFE and IFE, the opportunities for strength are greater than the threats and weaknesses. Anak Rokan Gold Shop must be more dominant in carrying out strategies by growing and developing products.
THE INFLUENCE OF WORK ENVIRONMENT ON EMPLOYEE PERFORMANCE AT PT. MESITECHMITRA PURNABANGUN DURI-RIAU Desriani, Desriani; Adriani, Muhammad
Journal of Islamic Banking, Economics and Finance Vol. 1 No. 4 (2025): Juli 2025
Publisher : EL-EMIR Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63477/jibef.v1i4.278

Abstract

This study aims to analyze how the work environment influences employee performance at PT. Mesitechmitra Purnabangun. How to improve employee performance by considering the work environment within the company. The method used in this study is a survey, this type of research is quantitative. The number of samples in this study was 46 people, using a saturated sampling technique where all populations were sampled. The results of this study indicate that the work environment has a significant positive effect on employee performance at PT. Mesitechmitra Purnabangun Duri-Riau branch, seen from the T Statistic value of 11,698> 1,684 which illustrates that the work environment influences employee performance. And also strengthened by the results of the Determination Coefficient (R2) of 0.778 which contains the understanding that the influence of the work environment on employee performance at PT. Mesitechmitra Purnabangun is 77.8%.
Pengaruh Bantuan Langsung Tunai (Blt) Terhadap Peningkatan Ekonomi Masyarakat Di Desa Kijang Jaya, Kecamatan Tapung Hilir, Kabupaten Kampar Fitri, Fitri; Marabona Munthe; Desriani, Desriani; Dwiana Indah Lestara; Edi Haryono
EKOMA : Jurnal Ekonomi, Manajemen, Akuntansi Vol. 5 No. 3: Maret 2026
Publisher : CV. Ulil Albab Corp

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56799/ekoma.v5i3.15785

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh Bantuan Langsung Tunai (BLT) terhadap peningkatan ekonomi masyarakat di Desa Kijang Jaya. Bantuan Langsung Tunai merupakan salah satu program pemerintah yang bertujuan untuk membantu masyarakat miskin atau terdampak ekonomi, terutama akibat situasi krisis seperti pandemi atau kondisi ekonomi lainnya. Penelitian ini menggunakan pendekatan kuantitatif dengan metode survei dan teknik analisis data regresi linear sederhana. Data dikumpulkan melalui penyebaran kuesioner kepada 48 responden yang menerima BLT. 1. Berdasarkan analisis secara parsial, hasil uji hipotesis menunjukkan thitung (5,638) > ttabel (1,678) dengan sig 0,000 < probabilitas 0,05 yang berarti hal ini Ho ditolak Ha diterima, sehingga dapat disimpulkan bahwa ada pengaruh yang signifikan Bantuan Langsung Tunai (BLT) terhadap Peningkatan Ekonomi Masyarakat pada pada Desa Kijang Jaya,Kecamatan Tapung Hilir,Kabupaten Kampar. Berdasarkan perhitungan koefisien Determinasi (R2) diketahui bahwa nilai R Square sebesar 0,682. Hal ini menunjukkan bahwa variabel Bantuan Langsung Tunai (BLT)secara keseluruhan memberikan pengaruh sebesar 68,2% terhadap Peningkatan Ekonomi Masyarakat. Hasil penelitian menunjukkan bahwa BLT memiliki pengaruh positif dan signifikan terhadap peningkatan ekonomi masyarakat, yang ditunjukkan melalui peningkatan daya beli, konsumsi rumah tangga, dan stabilitas ekonomi keluarga penerima manfaat. Dengan demikian, program BLT dapat dikatakan efektif dalam membantu meringankan beban ekonomi masyarakat di wilayah penelitian.
Co-Authors Abna, Inherni Marti Adi Santoso Agustiyanti, Dian Fitria Andri Wardiana, Andri Atikana, Akhirta Azamris, Azamris Azamris, Azamris Baehaqi Budiarto, Bugi R BUGI RATNO BUDIARTO Chaeriyah, Naeilul Dwi Endah Kusumawati Dwi Wulandari Dwiana Indah Lestara Edi Haryono ERNAWATI ARIFIN GIRI-RACHMAN Ferry Iskandar Fibriani, Azzania Filasofa, Lilif Muallifatul Khorida Fitri Fitri Hapsari, Betalini Widhi Harahap, Risalan Basri Harahap, Wirsma A Hariyatun, Hariyatun Hasibuan, zulfan Efendi Haz, Aufa Rizqia Isna Nurhayati, Isna Jumardi Jumardi Kumala, Ajeng Putri Kusharyoto, Wien LINDA SUKMARINI MARIA BINTANG Marissa Angelina Muhammad Adriani, Muhammad Munawarah Munthe, Marabona Mursid Mursid Neneng Hasanah Neng Herawati, Neng Noorohmah, Siti Okasa, Andi Muliarni Permatasari, Fitri Aulia Popi Hadi Wisnuwardhani, Popi Hadi Prasetyoputri, Anggia PUSPITA LISDIYANTI Putra, Masteria Yunovilsa Putri Kumala, Ajeng Rahayu, Resa Sri Raihan, Eldrian Daffa Rantau, Deritha Ellfy RATIH ASMANA NINGRUM Rinal Effendi Riyona Desvy Pratiwi, Riyona Desvy Siti Zubaidah Sitti Fatimah Syahid Sofa Muthohar Stephanie, Rebecca Sulaiman Sulaiman Syamsul Falah Taharuddin, Audrey Angelina Putri Wahyudi, Denny Warisman, M Ali Warisman, M. Ali Wiwit Amrinola Wulandari, Dyah Retno Yamahoki, Nicholas Yana Rubiyana, Yana Yuliawati Yurika, Oktri