Article Per Year (5 Year)
p-Index From 2021 - 2026
3.366
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Silvikultur Tropika (JST) Agrotrop : Journal on Agriculture Science Bionatura Zuriat Jurnal Natur Indonesia Atom Indonesia Journal Jurnal Ilmiah Aplikasi Isotop Radiasi Jurnal Agro Teknologi Tropika Jurnal Ilmu Komputer dan Agri-Informatika Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Pelita Perkebunan Jurnal Agroekoteknologi Jurnal Agro Jurnal Penelitian Karet Tropical Animal Science Journal Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Journal of Tropical Crop Science JURNAL BUDIDAYA PERTANIAN Jurnal Agrotek Lestari Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Warta Perkaretan Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Jurnal Natural
Desta Wirnas
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Neuroscience Physics Social Sciences Veterinary

Published : 79 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : HAYATI Journal of Biosciences

 1 
Characterization of cDNA for PMT: a Partial Nicotine Biosynthesis-Related Gene Isolated from Indonesian Local Tobacco (Nicotiana tabacum cv. Sindoro1) SESANTI BASUKI; NURHAJATI MATTJIK; DESTA WIRNAS; . SUWARSO; . SUDARSONO
HAYATI Journal of Biosciences Vol. 20 No. 4 (2013): December 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (807.08 KB) | DOI: 10.4308/hjb.20.4.187-195

Abstract

Nicotine is the major alkaloid compound in cultivated tobacco (Nicotiana tabacum) that could potentially be converted into carcinogenic compound (nor-nicotine). The PMT gene encoding putrescine N-methyltransferase (PMT) is one of the two key genes that play a prominent role in nicotine biosynthesis. The aimed of this study was to isolate and characterize the cDNA sequence originated from Indonesian local tobacco cv. Sindoro1 (Ntpmt_Sindoro1). The results showed that the Ntpmt_Sindoro1 was 1124 bp in length. This cDNA fragment encodes for 374 amino acid residues. The predicted polypeptide from the cDNA is a hidrophilic protein, and has a predicted molecular weight of 40.95 kDa. The predicted amino acids sequence also showed high similarity to the PMT gene product Nicotiana sp. available in the GenBank data base. The amino acid sequences also exert conserved residues specifically exhibited only by PMT gene originated from N. tabacum. Clustering analysis revealed that Ntpmt_Sindoro1 belongs to the same clade as the PMT3 gene, a member of the N. tabacum PMT gene family. The Ntpmt_Sindoro1 cDNA sequence covering exon1-exon8 of the PMT gene fragment has been registered in the GenBank data base, under the accession number JX978277.
Co-Authors ,, Mawaddah . SUDARSONO . SUWARSO Achmad . Adinda Wuriandani Adinda Wuriandani Agung Wahyu Susilo Agung Wahyu Susilo Agung Wahyu Susilo Ahmad Ansori Mattjik Ahmad Ansori Mattjik AHMAD JUNAEDI Aidi Noor Akhmadi, Gerland Alaydi, Nafian Amris Makmur Anita Rahayu Arvita Netti Sihaloho, Arvita Netti Atika Bakti Sari Azri Kusuma Dewi Bambang Sapta Purwoko Buang Abdullah Dedek hanafiah Dewi Andriani Diana Sofia Hanafiah Didy Sopandie Edi Santosa Edison Jambormias EDIZON JAMBORMIAS Eka Bobby Febrianto, Eka Bobby Eni Widajati Faqih Udin Faradila Median Rini Firman Ardiansyah Gopar, R. A. HAJRIAL ASWIDINNOOR Halimah Tus Sa'diah Halimatus Syahdia Hasibuan HESTI MAULIDA Hidayatun, Nurul Human, dan Soeranto I. Prihantoro, I. Imam Widodo Ishmatalhaq, Zannuba Iskandar Lubis Iswari S. Dewi Iswari Saraswati Dewi Jaisyurahman, Usamah Jasmi Jekki Irawan Junaedi, dan Ahmad Karlin Agustina Khairil Anwar L. Abdullah Lela Marlenasari M A Chozin Mahpuzah, Marfiatun Manalu, Victor Manotar Pademan Mardhiyyah, Rodhiyatan Marlenasari, Lela Maryono, Marina Yuniawati Mattjik, Ahmad A Maulida, Hesti Mayang Sari Memen Surahman Mizan, Muhammad Rauful Mizan, Muhammad Rauful Momongan, Jorex Daniel Muhamad Syukur Muhammad Arif Yudiarto Munarti Munif Ghulamahdi Nindita, Anggi Nita Kartina, Nita Nofika Senjaya NUR KHOLISOH NURHAJATI AA MATTJIK NURHAJATI MATTJIK Nurheni Wijayanto OTIH ROSTIANA P. D. M. H. Karti Patty, Jacob R Pipit Werdhiwati Prasetiyono, dan Joko Prasetiyono, Joko Pratama, Muhammad Antony Jefri Purbokurniawan . Putra, B. Putri Andini Mandasari Ratih Irma Khairani Saragih REFLINUR REFLINUR Rini, Erin Puspita Rini, Faradila Median S. Human Saniaty, Arina Saragih, Ery Leonardo Saragih, Ratih Irma Khairani Sayurandi Sayurandi Sayurandi Sayurandi Sayurandi Sayurandi Sayurandi Sayurandi, Sayurandi Saļ¬tri, Heni Sekar Woelan Sekar Woelan Sekar Woelan Sekar Woelan SESANTI BASUKI SESANTI BASUKI Sherly Rahayu Siti Marwiyah Siti Marwiyah Sobir Sobir Sobir Sobir Sobir Sobir Soeranto Human SRI WARDANI Sudarsono SUDARSONO SUDARSONO Sudarsono, dan Sudirman Yahya Sudirman Yahya Sudirman Yahya Sudirman Yahya - Sumiati, dan Sungkono Sungkono Sunyoto Sunyoto Surjono H Sutjahjo, Surjono H Surjono Hadi Sutjahjo Suwarno, Punjung Medaraji SUWARSO SUWARSO Tasliah, nFN Tias Arlianti Tri Budiyanti Trikoesoemaningtyas Trikoesoemaningtyas Trikoesoemaningtyas Trikosoemaningtyas Trikosoemaningtyas Trikosoemaningtyas Usamah Jaisyurahman W. Puspitasari Wage Ratna Rohaeni WAGE RATNA ROHAENI Wardhani, Azhahara Putri Kusuma Wibawa, Rentang Fajar Cakra Wuriandani, Adinda Yani Nurhadryani Yudiwanti Wahyu E. Kusumo Yulidar Yulidar