Article Per Year (5 Year)
p-Index From 2021 - 2026
3.291
P-Index
This Author published in this journals
All Journal Jurnal Sain Veteriner BALI MEDICAL JOURNAL (BMJ) Archive of Community Health Itepa : Jurnal Ilmu dan Teknologi Pangan INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES E-Jurnal Medika Udayana Indonesia Medicus Veterinus Bali Journal of Anesthesiology Journal Of Vocational Health Studies Oceana Biomedicina Journal ISM (Intisari Sains Medis) : Jurnal Kedokteran Interdental Jurnal Kedokteran Gigi (IJKG) Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) OISAA Journal of Indonesia Emas Bali Dental Journal Oceana Biomedicina Journal Indonesian Journal of Cancer INDONESIAN JOURNAL OF URBAN AND ENVIRONMENTAL TECHNOLOGY Journal of Global Pharma Technology Neurona Indonesian Journal of Pharmacology and Therapy
I Dewa Made Sukrama
Laboratorium Mikrobiologi, Fakultas Kedokteran, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Dentistry Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

Published : 56 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search
Journal : Journal of Global Pharma Technology

 1 
Inverse Correlation between Combination of Antiretroviral Therapy with the level of Serum Interleukin-6, Ferritin, and Hepcidin in HIV Patients with Anemia Chronic Disease I Ketut Agus Somia; Ketut Tuti Parwati Merati; I Made Bakta; Ida Bagus Putra Manuaba; Wayan Putu Sutirta Yasa; I Dewa Made Sukrama; Ketut Suryana; Rudi Wisaksana
Journal of Global Pharma Technology .
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: The purpose of this study was to determine whether the cARV therapy correlates with the serum level of IL-6, ferritin, and hepcidin in HIV infected patients with ACD.Methods: A cross-sectional study conducted in HIV patients with ACD, aged 15-65 years with exclusion criteria such as chronic renal disease, chronic liver disease, treated for anemia in the last 3 months, taking iron supplements in the last 3 months, history of blood transfusion in the last 1 year, suffering from acute infection, tuberculosis infection, malignancy, hepatitis C virus infection, acute hypersensitivity reaction and pregnancy. Blood specimen examined by high sensitivity human IL-6 In vitro ELISA, DRG Hepcidin- 25 ELISA, serum ferritin by agglutination method and CD4 cell count by flow cytometry. Results: A total of 86 HIV patients with ACD consist of 42 subjects with experienced cARV and 44 subjects with naive cARV. There are several differences between cARV naïve and experienced cARV including: Hb (g/dl) [ 10.8 ( 9.88 – 11.75) Vs. 11.60 (11.00 – 12.34) p: 0.058 ], serum iron (mg/dl) [ 50.00 (31 – 66.8) Vs. 71.40 (56.00 – 108.00) p: 0.121 ), serum ferritin (ug/dl) [ 630 ( 194 – 1101) Vs. 195.40 (128.25 – 589.47) p: 0.008 ], IL-6 (pg/mL) [7.20 (3.88 – 10.76) Vs. 4.03 (1.69 – 8.49) p: 0.009), serum hepcidin (ng/mL) [45.54 (16.74 – 95.31) Vs. 23.35 (6.58 – 49.99) p: 0.007] , CD4 cell count (cell/uL) [31.5 (14.25 – 90.75) Vs. 307 (168.5 – 458.75) p : < 0.001)] There is a significant inverse correlation between the cARV therapy with level of serum IL-6 ( r: - 0. 285, p: 0.008), ferritin (r: - 0.321, p: 0.007) and hepcidin (r: - 0.293, p: 0.006). While there was a significant positive correlation between the cARV therapy with CD4 cell count (r: 0.676, p: 0.001). There was no correlation between the cARV therapy with serum iron levels ( r: 0.176, p: 0.121).Conclusion: There is an inverse correlation between the cARV therapy with serum level of IL-6, ferritin, and hepcidin in HIV patients with ACD.  Keywords: cARV, ACD, IL-6, Ferritin, Hepcidin.
Determination of lactic acid bacteria isolated from the gastrointestinal tract of Bali cattle with potential probiotic role Dewa Made Sukrama; I Wayan Suardana
Journal of Global Pharma Technology Volume 10 Issue 08: (2018) August 2018
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bali cattle is known as pioneer’ cattle resulted by their high ability to adapt in poor quality of feed so that was assumed for finding the specific species of lactic acid bacteria.  The study aimed to investigate the potency of lactic acid bacteria isolated from the gastrointestinal tract of Bali’ cattle as a candidate for new probiotic. Fifteen isolates of lactic acid bacteria isolated from 15 intestinal tracts of Bali cattle were grown on the specific medium deMan Rogosa Sharpe (MRS) broth followed by Gram staining, catalase test, the formation of CO2 gas, and antimicrobial activities against Bacillus cereus. The result of this study confirmed 15 isolates were Gram-positive bacteria, and 14 out of 15 isolates were categorized as homofermentative. Probiotic potency test showed 11 out of 15 isolates were tolerant against lower pH (pH 2, 4 and 6), and also resistant to bile acid with NaDC concentration 0.2, 0.4, and 0.6 mM.  Identification of isolate using API 50CH test kit found 4 out of 5 isolates as Lactobacillus sp. and the remainder as Lactococcus sp. The study concluded isolates of lactic acid bacteria isolated from the gastrointestinal tract of Bali cattle showed potential as a probiotic candidate.
High Expression F2-Isoprostan (F2-IsoP), High Sterol Regulatory Element Binding Protein-2 (SREBP-2) and Low 2-Methoxyestradiol (2-ME) On Placenta Tissue as a Risk Factor of Pre-Eclampsia Jaya Kusuma AAN; Suwiyoga K; Mantik AN; Sukrama DM
Journal of Global Pharma Technology Volume 09 Issue 09
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

To date, pre-eclampsia (PE) still a problem of Maternal Fetal Medicine related to high incidence, maternal and neonatal morbidity and mortality. Pre-eclampsia is caused by pregnancy; however, the mechanism has not been established so it is still a disease of theories. This relates to differences in treatment, resulting in different ways of prevention and output of PE itself. Recently, the role of F2-IsoP ,SREBP-2 and 2-ME was suspected to be very important in the mechanism of the PE. Meanwhile, the placenta acts as a source regulatory protein production, so that the material of this study was taken from placental tissue. The study objective was to prove the high F2I-soP, high SREBP-2 and low 2-ME expression in the placenta as risk factors for PE Case control study has been conducted in the department of obstetrics and gynecology Sanglah Hospital with 62 samples in 2015. The case group consisted of 31 mothers with PE and control groups consisted of 31 non-PE mother. The study material is a placental tissue. F2-IsoP and SREBP-2 expression study was performed using immunohistochemistry and 2-ME with ELISA techniques in Pathobiology Laboratory Faculty of Veterinary Medicine Udayana University. Data were analyzed with chi square test and discriminant using SPSS. The statistical test results are presented in tabular form and narrative. In this study, it was found that high expression of F2-IsoP increased the risk of PE  4 times higher (OR = 4.44; 95% CI = 1.53 to 12.94; p = 0.005) ; high expression of SREBP-2 increased the risk of PE 8 times higher  (OR = 8.19, CI95% = 2,311 to 29.073; p = 0.001) and low expression of 2-ME increased the risk of PE 5 times higher (OR = 5.23; CI95% = 1.75 to 15.55; p = 0.002). On the discriminant test, we obtained contributing risk factor for the occurrence of PE were SREBP-2, F2Isop and 2-ME (p = 0.002) respectively. Conclusion, high F2-IsoP expression, high SREBP-2 expression and low 2-ME expression in placenta were risk factors for PE. The most dominant risk factor of PE mechanism was SREBP-2.Keywords: Pre-eclampsia, F2-Isoprostane, Sterol Regulatory Element Binding Protein-2, 2-Methoxyestradiol.
MOLECULAR ANALYSIS OF LACTIC ACID BACTERIA ISOLATE SR2 FROM BALI CATTLE RUMEN I Dewa Made Sukrama; Komang Januartha Putra Pinatih; I Wayan Suardana
Journal of Global Pharma Technology Volume 09 Issue 05
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bali cattle with its distinctively genetic characteristics, namely simple life or easy to adapt on unfavorable environmental condition was known as cattle pioneer. These characteristics were due to specific type of lactic acid bacteria (LAB). These facts indicate that the LAB identification with specific advantages was absolutely necessary for further research in order to prevent incorrect interpretation. Generally, molecular approach is the accurate method of identifying microorganism, and in this case, we used the analysis of the 16S rRNA gene. Research activities began with cultivation of SR2 isolates on specific media deMann Rogosa Sharpe (MRS) followed by Gram stain, catalase test, and formation of CO2 gas. Molecular analysis was performed by PCR of the 16S rRNA gene using primers 27F and 1492R. The PCR products were sequenced and nucleotides data were analyzed by Mega 5.2 Software tool. The results showed that phenotypical isolates of SR2 showed characteristics as LAB isolates, and the results of the 16S rRNA gene analysis showed that isolates SR2 included into Lactococcus lactis strain with a bootstrap value of 100.Keywords - Bali cattle, lactic acid bacteria, molecular analysis, rumen, SR2 isolate
Co-Authors AA. Raka Karsana Adinda Putra Pradhana Adiputra, I Komang Hotra Amin, Yusuf Sidang Anak Agung Ngurah Jaya Kusuma Anggita Ratri Pusporini Astawa N. M., Astawa N. Astawa P., Astawa Bagus Komang Satriyasa Biantara, I Wayan Arya Cokorda Agung Wahyu Purnamasidhi Darren Junior Dennis Yulianto Desak Gde Diah Dharma Santhi Dewa Ayu Fony Prema Shanti Dewa Ayu Putu Rasmika Dewi Dewa Gede Agung Widyadnyana Dewa Ngurah Suprapta Dewa Ngurah Suprapta Dewa Putu Satria Juristiasa Dewi Anggraini Dik Megaputri Handayani Ekaputra Ekaputra, Ekaputra Ema Surya Pertiwi Gema Zakharian Hendrawan, Gresya Hervina Hervina I D.P. Kartika Pratiwi I Dewa Ayu Agung Warmadewanthi I Dewa Gde Mayun Permana I Gede Purna Weisnawa I Gusti Agung Ayu Anjani Kartika Dewi I Gusti Agung Dyah Ambarawati I Gusti Ayu Fienna Novianthi Sidiartha I Gusti Ayu Mas Putri Dharmayanti I Gusti Ngurah Kade Mahardika I Ketut Agus Somia I Ketut Suwiyoga I Komang Hotra Adiputra I M. Sumarya, I M. I Made Bakta I Made Gustama Heryawan I Made Jawi I Made Reza Pramudya I Made Sathya Vijayananda I Nengah Sujaya I NYOMAN MANTIK ASTAWA I Wayan Adi Pranata I Wayan Arya Biantara I Wayan Muda Suta Arta I Wayan Putu Sutirta Yasa I Wayan Suardana I Wayan Suranadi I. K. Sukardika I.D.A.A Warmadewanthi I.K. Suata Ichlazul Ma’ruf Ida Bagus Nyoman Putra Dwija Ida Bagus Putra Manuaba Ida Sri Iswari Jerry Jerry Junior, Darren K. Sukardika Kadek Diana Harmayani Kadek Tresna Yuwana Ketut Suryana Ketut Tuti Parwati Merati KOMANG AYU NOCIANITRI Komang Ayu Witarini Komang Januartha Putra Pinatih Luh Wayan Ayu Rahaswanti Made Agus Dwianthara Sueta Made Agus Hendrayana Mantik AN Mia Ayustina Prasetya N. Adiputra Ni Kadek Fiora Rena Pertiwi Ni Kadek Lyming Lestari Ni Luh Putu Harta Wedari Ni Made Adi Tarini Ni Made Ayu Aryati Dinarini Ni Made Susilawathi Ni Nengah Dwi Fatmawati Ni Nengah Dwi Fatmawati Ni Nyoman Puspawati Ni Nyoman Sri Budayanti Ni Wayan Arni Sardi NYOMAN SEMADI ANTARA Pramitasuri, Tjokorda Istri Putra, I Dw. Gd. Bayu Artha Pratama Putu Anda Tusta Adiputra Putu Aprilyana Eka Astuti Putu Bagus Redika Janasuta Putu Kintan Wulandari Putu Wiswananta Parama Raka-Sudewi A. A. Reny Rosalina Rian Ka Praja Rudi Wisaksana Saranova, Hilda Saraswati P. Yogita Satoto D., Satoto Shanti, Dewa Ayu Fony Prema Sherly Yunita Sieny Veronica Sonia Elvira Salim Sri Maliawan Steffano Aditya Handoko SUMARNO Suryadi N. T., Suryadi N. Susilawathi, Ni Made Tjokorda Gde Agung Senapathi Tjokorda Gde Bagus Mahadewa W. A., W. Wahyu Hidayati Wayan Redi Aryanta Weisnawa, I Gede Purna Wibisana, I Dewa Nyoman Adi Ningrat Winatha, I Gde Pangestu Putrama Wira Guna, I Gede Bhima Wiryana M., Wiryana Wulandari, Putu Kintan Yan Ramona