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All Journal Biotika: Jurnal Ilmiah Biologi Microbiology Indonesia International Journal of Integrated Health Sciences Majalah Kedokteran Bandung Jurnal Kesehatan Andalas Biology, Medicine, & Natural Product Chemistry Padjadjaran Journal of Dentistry Paediatrica Indonesiana Jurnal Ilmiah Kesehatan Anakes: Jurnal Ilmiah Analis Kesehatan Journal Medical Informatics Technology
Prima Nanda Fauziah
Universitas Mohammad Husni Thamrin
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Gambaran Kadar Hemoglobin Pada Pasien Malaria di RSPAD Gatot Soebroto Jakarta Indonesia Damayanti, Vila; Permana, Atna; Fauziah, Prima Nanda; Susanti, Ellis; Latifah, Imas; Masdianto
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 1 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i1.2669

Abstract

Malaria is both an acute and chronic disease caused by protozoa of the genus Plasmodium with the clinical manifestation of fever, anemia and spleen enlarfication. People with malaria, which the plasmodium attacks, are red blood cells. Malaria infections destroy erythrocytes, which results in a change in hemoglobin levels far lower than normal value. This study aims to find out the hemoglobin level in malaria patients, knowing the distribution of the number of malaria patients and the hemoglobin levels based on gender, age, and type of Plasmodium sp. The study employed a secondary descriptive method of data analysis by calculating the distribution of hemoglobin levels presented in the form of tables and narratives, the data used by up to 116 samples of malaria patients. Data retrieval took place at rspad gatot soebroto lab with populations and samples from 2013- 2023. Obtained from a hemoglobin level check in malarial patients shows a value of min 7.0 gr/dl, Max 16.9 gr/dl, mean 12.6 gr/dl, low hemoglobin level 82 patients (70.7%), normal 31 patients (26.7%), 3 (2.6%), the highest distribution of the number of patients in 2022 (15.5 percent). With the largest abnormal measured measured in men by 75 patients (64.2%), the results of a lower level check at 19- 44 (adults), with 64 patients (55.2%), the most malaria patients suffer from plasmodium vivax with a lower percentage of 51 patients (44.0%). In conclusion, that the most malaria affected men, an age susceptible to 19-44 (adult) malaria, and most malaria patients have plasmodium vivax infected.  Keywords : Hemoglobin, gender, malaria, Plasmodium sp, age
Gambaran Hasil Pemeriksaan Penderita Demam Tifoid dengan Metode Widal di Laboratorium Kesehatan Daerah Kota Sukabumi Zaranggi, Ahmad; Fauziah, Prima Nanda; Widhyasih, Retno Martini
Jurnal Ilmiah Kesehatan Vol 16 No 2 (2024): Jurnal Ilmiah Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/jik.v16i2.2240

Abstract

Typhoid fever is a systemic disease that becomes a world health problem. Typhoid fever occurs in both tropical and sub-tropical countries, especially in developing countries. To determine the exact diagnosis of this disease required examination Widal test. typhoid characterized by prolonged heat followedby bacteremia and bacterial invasion of Salmonella sp. The study aims to determine whether the diagnosis of Typhoid Fever Suspect isappropriate with the Widal Serology Test. The type of this research is descriptive research. Population of this research is outpatient in Sukabumi city health laboratory patients with typhoid fever in august 2020 – april 2021. The sample in this study were as many as 30 respondents. The results showed that the percentage of Widal Serology Test on positive Typhoid Fever was 18 people and 12 people with negative Widal Serology Test results. Positive results for Salmonella typhi O = 9 samples, Salmonella typhi H = 8 samples, Salmonella paratyphi AO as many as 9 samples, with positive results for Salmonella paratyphi BO as many as 11 samples, with positive results for Salmonella paratyphi CO as many as 13 samples, with positive results for Salmonella paratyphi AH as many as 7 samples, with a positive result of Salmonella paratyphi BH as many as 8 samples, with a positive result of Salmonella paratyphi CH as many as 7 samples. The conclusion of this study for the Widal Serology Test in positive Typhoid Fever was around 60%.The conclusion of the writing of this is more a hepatitis b patients (100%) men than women with age 30 - 65 years, decline and a rise in the number of platelets and SGPT and SGOT levels .
Karakteristik Sifat Fisik, Nilai Gizi serta Mikrobiologi Soyghurt Fauziah, Prima Nanda; Latifah, Imas; Manikam, Ratna Mutu; Masdianto, Masdianto; Nugroho, Heru Purwanto
Jurnal Ilmiah Kesehatan Vol 16 No 2 (2024): Jurnal Ilmiah Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/jik.v16i2.2414

Abstract

Soyghurt merupakan produk fermentasi dari susu kedelai. Salah satu bakteri yang digunakan untuk memfermentasi susu kedelai adalah Lactobacillus bulgaricus. L. bulgaricus merupakan salah satu bakteri asam laktat (BAL) atau probiotik yang memiliki manfaat bagi kesehatan tubuh manusia dan merupakan salah satu bahan baku susu yang paling banyak digunakan sehingga dapat dikonsumsi di seluruh dunia. Bakteri ini merupakan Lactobacillus pertama yang diumumkan sebagai bakteri yang mampu memfermentasi susu dan berbagai produk fermentasi lainnya. Bakteri ini telah terbukti aman digunakan serta memiliki efek probiotik yang dapat mengatasi kondisi intoleransi laktosa. Produk fermentasi berbasis tanaman saat ini mengalami perkembangan yang cukup besar, sebagai hasil dari evolusi kebiasaan konsumen, dalam konteks umum transisi pangan. Hal ini memerlukan penelitian dan pengembangan, dan dengan demikian pengetahuan ilmiah, untuk memungkinkan transisi tersebut, termasuk pengembangan susu kedelai yang difermentasi dengan penambahan bakteri L. bulgaricus atau dikenal dengan sebutan soyghurt. Tujuan dari penelitian ini adalah untuk mengetahui nilai gizi, karakteristik sifat fisik dan kimia dari susu kedelai yang difermentasi dengan Lactobacillus bulgaricus. Penelitian ini menggunakan metode eksperimental di Laboratorium Kimia dan Mikrobiologi dan uji mutu hedonik untuk menilai aroma, rasa dan warna dari soyghurt dengan rancangan acak lengkap (RAL) monofaktorial 11 perlakuan konsentrasi soyghurt dan 3 kali pengulangan. Hasil penelitian menunjukkan bahwa kandungan nilai gizi susu kedelai per 100 g memiliki energi sebesar 322,6 kkal, protein 15,7 g, lemak 9,9 g, karbohidrat 42,1 g, serat 7,5 g, kolesterol 0 mg dan sodium 1 mg, sedangkan soyghurt per 100 g memiliki energi sebesar 617,6 kkal, protein 54 g, lemak 14,5 g, karbohidrat 80,3 g, serat 28,5 g, kolesterol 0 mg, sodium 51 mg, dan kalium 2000 mg. Soyghurt pada konsentrasi 60% memiliki rasa, warna dan aroma yang banyak disukai oleh panelis. Fermentasi susu kedelai dengan L. bulgaricus dapat meningkatkan nilai protein dan lemak pada susu kedelai, serta memberikan rasa yang lebih enak.
Perbedaan Kadar Kolesterol Total Metode CHOD-PAP Dalam Sampel Serum Segera Dan Ditunda Selama 2, 4, 6 Jam Pada Suhu Ruang Pratiwi, Isnaini Nisa Restu; Susanti, Ellis; Fauziah, Prima Nanda; Latifah, Imas; Masdianto
Jurnal Ilmiah Kesehatan Vol 17 No 2 (2025): Jurnal Ilmiah Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/jik.v17i2.2847

Abstract

Cholesterol examination is important to monitor the presence or absence of abnormalities in fat metabolism characterized by an increase or decrease in fat, the sample used for cholesterol examination is fresh serum and immediately examined. This study aims to determine the difference in total cholesterol levels CHOD-PAP method in serum samples immediately and delayed for 2, 4, 6 hours at room temperature. This research uses experimental designs with a cross sectional design which is tested on 40 samples of Regular Students of Semester 6 of the DIII Medical Laboratory Technology Study Program at Mohammad Husni Thamrin University. The sample examination method is done by taking venous blood and processed into serum. Research results that have been statistically tested on the Friedman test can be concluded for serum samples immediately and delayed for 2, 4, 6 hours with a significant value of 0.711> α (0.05), there is no difference in the results of the examination of total cholesterol levels with immediate work and a delay of 2, 4, 6 hours. In the percentage test, it was concluded that there was a decrease in the results of checking cholesterol levels in serum samples delayed 2, 4 hours by 2.9% and 5.7% in serum samples delayed 6 hours. Total cholesterol examination should be carried out immediately without delay. If the examination is delayed, the storage temperature and how long the delay should be considered. Keywords: CHOD-PAP, Cholesterol, Serum, Delay Time
Activity of Antimycobacterial Candidate from Bacteriocins and Lactic Acid Isolated from Lactobacillus bulgaricus Against Mycobacterium tuberculosis Prima Nanda Fauziah; Imas Latifah; Ratna Mutu Manikam; Masdianto Masdianto
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1605-1610

Abstract

Mycobacterium tuberculosis (Mtb) is a bacterium that causes a respiratory infectious disease known as tuberculosis. Tuberculosis (TB) is a global infectious disease that causes death. The current TB treatment method has a weakness, namely the tendency of patients to disobey the rules in taking drugs because they have to take several tablets of medicine for a long time. As a result, some strains of M.tuberculosis can strengthen themselves and become resistant to antimycobacterial drugs used during therapy. Based on these tuberculosis control problems, the WHO declared a global TB emergency.  The low effectiveness of currently used antitubercular drugs encourages the development of new antimycobacterial drugs with low side effects, fast-acting, broadly targeted, and can damage the virulence properties of M.tuberculosis so that the immune system can phagocytose M.tuberculosis. A recent solution from some researchers is to induce immunomodulatory effects that help during the tuberculosis infection process, including from lactic acid bacteria (LAB). This encourages researchers to reveal the interaction of probiotic L.bulgaricus on reducing the growth of M.tuberculosis by finding the main substance with antitubercular activity to obtain optimal results. This study used sensitive M.tuberculosis strain H37Rv to get complete preliminary data. This study tested lactic acid and bacteriocin of Lactobacillus bulgaricus at concentrations of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100%. M.tuberculosis bacteria were inoculated on media containing lactic acid and L. bulgaricus ATCC 11842 bacteriocin, then incubated at 37°C and observed every week from the fourth to the eighth week. Of the five concentrations of lactic acid and bacteriocin tested, only the 60-100% concentration could inhibit the growth of M.tuberculosis. In conclusion, lactic acid and bacteriocin of L. bulgaricus can hinder the growth of M.tuberculosis and can be developed into antimycobacterial drug candidates.
Karakterisasi Profil Leukosit Diferensial pada Penderita Tuberkulosis Paru sebagai Data Dasar Profil Imunologis Pasien TB Regina Parahita Agustin; Prima Nanda Fauziah; Cahyawati Rahayu; Lenggo Geni; Imas Latifah; Ellis Susanti; Masdianto Masdianto; Atna Permana
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 12 No. 1 (2026): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v12i1.3656

Abstract

Pulmonary tuberculosis (TB) remains a major global health problem with high morbidity and mortality rates. Infection with Mycobacterium tuberculosis triggers immune responses that can alter leukocyte distribution, making differential leukocyte counts valuable in assessing patients’ immune status. This study aimed to describe the leukocyte differential count results in pulmonary TB patients at RSUD Pasar Rebo during 2023–2025, analyzed by gender and age. A quantitative secondary data analysis was conducted by grouping leukocyte parameters based on sex and age, then presented in frequency distribution and percentages. The results showed that most patients TB had normal basophil values (99.6%). Eosinophils were almost equally distributed between normal (44.4%) and elevated (44.4%). Neutrophils were predominantly normal (58.7%), while lymphocytes tended to decrease (52%). Monocytes were mostly within the normal range (56.5%), although elevated levels were also frequent (43.5%). By gender, males more often showed elevated eosinophils, decreased lymphocytes, and high monocytes, whereas females more frequently exhibited elevated neutrophils and normal monocytes. In adolescents, all parameters were normal except for monocytes, which were elevated; in adults, lymphocytes were mostly low; and in the elderly, increased eosinophils and decreased lymphocytes were predominant. In conclusion, pulmonary TB patients demonstrated varied leukocyte patterns, particularly decreased lymphocytes and increased eosinophils and monocytes, reflecting immune responses to Mycobacterium tuberculosis infection. Keywords: Differential Count, Leukocytes, Pulmonary Tuberculosis
Co-Authors Aditia, Erie Affiyanti, Wirda Akhmad Yogi Pramatirta, Akhmad Yogi Alislam, Mochamad Arief Ani Melani Maskoen Aruna Gamarani Atna Permana Atna Permana Audia, Desi Basyir, Vaulinne Budi Handono Cahyawati Rahayu Catu Umirestu Nurdiani Chrysanti - Damayanti, Vila Dany Hilmanto Desi Audia Despiagia Pitaloka Dian Firmansyah Dida A. Gurnida DIKI HILMI Dzulfikar Djalil Lukmanul Hakim Ellis Susanti Ellis Susanti Erie Aditia Esti Istiqomah Fadil Oenzil Fakhrizal Hariyanto Wahdi Harsono Setiawan Heru Purwanto Nugroho IIS HERAWATI Imas Latifah Imas Latifah Imas Latifah Januar Rhamadani Jeffry Iman Gurnadi Jetty Nurhajati Jetty Nurhajati Johanes C. Mose Johanes C. Mose Latifah, Imas Lenggo Geni Lenggo Geni Lestari, Estu Mahmudah Mahmudah Masdianto Masdianto Masdianto Masdianto Masdianto Masdianto Masdianto Masdianto Masdianto Masdianto Masdianto Masdianto, Masdianto Mochamad Arief Alislam Mukti, Septiana Widya Murleni Dorawati Nugroho, Heru Purwanto Nurdiani, Catu Umirestu Permana, Atna Pitaloka, Despiagia Pratiwi, Isnaini Nisa Restu Rano Agustino Ratna Mutu Manikam Regina Parahita Agustin Retno Martini Widhyasih Septiana Widya Mukti Subastiono, Aditia Suhara, Intan Ryatni Syahrial Harun Syarifah Miftahul El Jannah Vaulinne Basyir Vira Amalia Wahdi, Fakhrizal Hariyanto Widhyasih, Retno Martini Widiarsih, Erlina Widiarsih, Erlina Wirda Affiyanti Yanwirasti Yanwirasti Yanwirasti Yanwirasti Yuliati, Tri Yuliati, Tri Yunia Qonitatin Al Masyani Zaranggi, Ahmad ZURAIDA ZURAIDA Zuraida Zuraida