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Journal : CHEMPUBLISH JOURNAL

Antioxidant Activity of Bioactive Compounds in the Ethyl Acetate Extract of Putat Leaves (Planchonia valida) Nelson; Silaban, Desy Vania; Farid, Faizar; Puspitasari, Ratih Dyah; Tarigan, Indra Lasmana; Ramadhan, Ilham Ifandi; Latief, Madyawati
Chempublish Journal Vol. 6 No. 4 (2022): Chempublish Journal
Publisher : Department of Chemistry, Faculty of Science and Technology Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/chp.v6i4.41632

Abstract

The Putat leaf (Planchonia valida) is a plant traditionally used by the community in the Tanjung Lanjut area, Jambi, both as a salad ingredient and as a traditional medicine to treat skin diseases and promote health. This identifies that the putat plant contains chemical compounds that potentially have antioxidant activity. In this study, putat leaf samples (Planchonia valida) that potentially have antioxidant activity. In this study, Planchonia valida (P. valida) leaf samples using n-hexane and ethyl acetate solvents through a multistep maceration process. Subsequently, phytochemical screening and antioxidant activity tests were conducted to obtain information about fractions that have the potential to be further isolated. The extraction percentage with a yield value of 1,28% was obtained in the maceration process. Further isolation was carried out using isolate liquid chromatography and FT-IR instruments. The obtained isolate was in the form of a wet solid, and through characterization, it was classified as a steroid compound suspected to be ergosterol. Free radicals or oxidants have reactive effects on the human body and can trigger various types of diseases. Antioxidants are known to have the ability to inhibit or neutralize free radicals in the human body. This study tested antioxidant activity using the DPPH (1,1-diphenyl-1,1-picrylhydrazyl) method on isolating F1. Isolate F1 was found to have antioxidant activity with a value of 714.637 ppm, falling into the range of weak antioxidant activity
Isolasi Proses Bioaktif Ekstrak Metanol Daun Putat (Planchonia valida) sebagai Antioksidan Farid, Faizar; Br Sembiring, Vera Lorensya; Nelson, Nelson; Saputra, Muhammad Furqon Novryan; Putri, Silvi Ayudiah; Tarigan, Indra Lasmana; Latief, Madyawati
Chempublish Journal Vol. 6 No. 4 (2022): Chempublish Journal
Publisher : Department of Chemistry, Faculty of Science and Technology Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/chp.v6i4.41634

Abstract

Putat leaves (Planchonia valida) are a plant that is traditionally used by people in the Tanjung Lanjut areas, Jambi as fresh vegetables, and as a traditional medicine to treat skin diseases and restore health. This identifies that the putat plant contains chemical compounds that have the potential to have antioxidant activity. Extraction was carried out using n-hexane and ethyl acetate solvents using multistage maceration. Next, phytochemical screening and antioxidant activity tests are carried out to obtain information about fractions that can potentially proceed to the isolation stage. In the maceration process, an extract percentage was obtained with a yield value of 1.39%. Next, isolation was carried out using vacuum liquid chromatography. The isolates obtained were then characterized using UV-Vis and FT-IR spectrophotometer instruments. The isolate obtained was in the form of a wet solid, and if seen through characterization, it belonged to the class of steroid compounds; it was suspected to be an ergosterol compound. Antioxidants are known to have the ability to inhibit and reduce the presence of free radicals in the human body. In this study, antioxidant activity was tested using the DPPH (2,2-diphenyl-1-picrihidazil) method on F1 isolates. The F1 isolate obtained was proven to have antioxidant activity with a value of 99.2265 ppm, which is classified in the strong antioxidant activity range.
Co-Authors -, Maison Afrianto, Muhammad Ficky Agnes Vionita Yohana Agus Syarif Alfi Khatib, Alfi Ali Nurdin Hidayat Arsa, Daniel Azira, Ayu Azril Bela, Anisa Br Sembiring, Vera Lorensya Daryati, Daryati Defia Indah Permatasari Denny Prabawa, Aditya Dewi, Tribuana Tungga Dhian Eka Wijaya Diah Riski Gusti Dwi Tata Damayanti Edwin Permana Edwin Permana Eka Nurshafni Erna Yustika Hutasoit Fandi Oktasendra Gusti, Diah Rizki Havizur Rahman Helga Dwi Fahyuan Helga Dwi Fahyuan Heriyanti Heriyanti Iful Amri Indra Lasmana Tarigan Intan Lestari Intan Lestari ira kusuma dewi Istiqomah Malinda Jesi Pebralia Juventa, Juventa Larasati, Nurrani Linda Handayani Madyawati Latief Mahraja, Mahra Mardian Peslinof Megakusuma, Yosha Mia Aina Muhammad Ikhsan Muhammad Ikrar Lagowa Nadea Arie Saragih Nelson Nelson Nelson Ngatijo Ngatijo Novianti Novianti Nurhidayah Nurhidayah Nurrani Larasati Oky Dwi Syaputra Prabawa, Satria Puspitasari, Ratih Dyah Putri Maya Sari Putri Maya Sari Putri, Silvi Ayudiah Rahman, Havizur Rahmat Basuki Ramadhan, Ilham Ifandi Resta, Ichy Lucya Restina Bemis Revis Asra Revis Asra Rikaldo Pratama Rikaldo Pratama Rustan Sampe Napitupulu Sampe Napitupulu Sampe Napitupulu, Sampe Samsidar Samsidar Samsidar Samsidar Samsidar Samsidar Samsidar Samsidar Samsidar Samsidar Samsidar Samsidar Saputra, Muhammad Furqon Novryan Sarinah Pakpahan Silaban, Desy Vania Sukor, Mohd Zaki Suwarni Suwarni Suwarni Suwarni Uce Lestari Uce Lestari Usna, Sri Rahayu Alfitri Wahyudi, Abdi Wahyudi, Lutfi Wibowo, Yudha Gusti Wiratama, Jarot Wiwid Joni Yohana, Agnes Vionita Yoza Fendriani Yuliawati Yusnaidar, Yusnaidar Zahar, Wahyudi