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Journal : Makara Journal of Technology

 1 
Digestibility of Betung Bamboo Fiber Following Fungal Pretreatment Fatriasari, Widya; Syafii, Wasrin; Wistara, Nyoman J.; Syamsu, Khaswar; Prasetya, Bambang
Makara Journal of Technology Vol. 18, No. 2
Publisher : UI Scholars Hub

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Abstract

This research evaluated the effect of fungal pretreatment of betung bamboo fibers and enzymatic- and microwaveassisted hydrolysis on the reducing sugar yield. The enzymatic hydrolysis of the pretreated biomass was carried out with cellulase and 10 and 20 FPU/g of substrate in a shaking incubator at 50 °C and 150 rpm for 48 h. The sulfuric acid concentration used in the microwave-assisted acid hydrolysis was 1.0, 2.5, and 5%, either with or without the addition of activated carbon. Microwave irradiation (330 Watt) was applied for 5–12.5 min. The yield of reducing sugar was better with the microwave-assisted acid hydrolysis, and the yield tended to increase with an increase in the irradiation time. Based on the dry weight of the initial biomass (bamboo), pretreatment with 5% inoculum loading resulted in a higher reducing sugar yield (17.06%) than with 10% inoculum loading (14.54%). At a 1% acid concentration, the formation of brown compounds decreased, followed by a reduction in the reducing sugar yield. The addition of activated carbon at a 1% acid concentration seemed to be of no benefit with respect to the yield in the microwaveassisted acid hydrolysis. The pretreatment with the 5% inoculum loading for 12.5 min at 1% acid concentration resulted in the highest reducing sugar yield. Under these conditions, the yield was 6.3-fold that of the reducing sugar yield using 20 FPU/g of cellulase. The rate of bamboo hollocellulose hydrolysis reached 22.75% of the maximum theoretical reducing sugar reducing sugar of dry biomass.
Co-Authors Agus Pratama, Andhika Putra Apip Amrullah Apri Heri Iswanto Apriastini, Marsiti Ashrianis, Devi Nury Asri Peni Wulandari, Asri Peni Bambang Prasetya Budiman, Ismail Danang Sudarwoko Adi Dede Heri Yuli YANTO Deni Zulfiana Dewi Selvia Fardhyanti Dewi Sondari Djarwanto Dyah Ayu Agustiningrum, Dyah Ayu Elviliana, Elviliana Euis HERMIATI Fahriya Puspita Sari Faizatul FALAH Fajriutami, Triyani Gutari, Sesmi Hadiyanto Hadiyanto hamzah, fajar nur Haniif Prasetiawan Himmi, Setiawan Khoirul I Nyoman Jaya Wistara Idrus, Syazwani Ina Winarni, Ina Irshad , Shagufta Ismayati, Maya Jung, Young Hoon Khaswar Syamsu Khorshidi, Fatemeh Hassani Kurnia Wiji Prasetiyo Laksana, Raden Permana Budi Listya Mustika Dewi Lubis, Muhammad Adly Rahandi Lucky Risanto Manggar Arum Aristri Mawarni, Rista Siti Meliana, Yenny Melville, Lynsey Muhammad Ghozali Muryanto Nafi'ah, Riris Waladatun Nafisyah, Ayu Lana Nanang Masruchin Narto Nissa Nurfajrin Solihat Nurhani Aryana, Nurhani Nurindah Obie Farobie Ogino, Chiaki Pari, Rohmah Pizzi, Antonio Pratomo, Bagas Ikhasan Rahman, Delicia Yunita Rahmanto, Raden Gunawan Hadi Ramadhan, Kharisma Panji Ratih Damayanti Riksfardini Annisa ERMAWAR Rohma, Novita Ainur Rusumayanti, Felli S Heris Anita, S Heris Saadatabadi, Ahmad Ramazani Sabrina Sunyoto, Nimas Mayang Septiyanti, Melati Setyawan, Hendrix Yulis Shahnaz Sita Heris Anita Sofianto, Imran Arra'd Sohail, Asma Sri Suhartini Supriyanto Supriyanto Suryanegara, Lisman Swastika Praharyawan, Swastika Teguh DARMAWAN Triwibowo, Dimas Wahyu DWIANTO Wahyu Hidayat Wasrin Syafii Witta Kartika Restu Yadav, Sumit Manohar Yusup AMIN Zia, Sobia