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Pemeriksaan Bone Mineral Density dan Pelatihan Senam Osteoporosis Sebagai Upaya Peningkatan Kewaspadaan Osteoporosis pada Penderita Talasemia Mayor Nyoman Suci Widyastiti; Edward Kurnia Setiawan Limijadi; Meita Hendrianingtyas; Ariosta Ariosta; Dwi Retnoningrum; Dwi Ngestiningsih; Yetty Movieta Nency
Journal of Dedicators Community Vol 5, No 2 (2021)
Publisher : Universitas Islam Nahdlatul Ulama Jepara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34001/jdc.v5i2.1348

Abstract

Thalassemia major patients with routine transfusions are at risk of experiencing iron overload and bone turn over disorders which will increase the risk of osteoporosis. Community training activities with BMD examinations and osteoporosis exercise training in thalassemia major patients are expected to identify the prevalence of bone fragility in thalassemia major patients, increase the awareness of thalassemia major patients and their families to the risk of osteoporosis in thalassemia major patients and osteoporosis exercise training as an effort to prevent osteroporosis. The results of BMD on the spine showed 57.1% osteoporosis, 28.6% normal and 14.3% osteopenia. The results of BMD examination on the cervical spine showed 71.4% osteopenia, 21.4% normal and 7.2% osteoporosis. The results of BMD examination on the femur found 50% normal, 42.8% osteopenia and 7.2% osteoporosis. The bone location with the highest percentage of osteoporosis was the spine (57%), and the bone location with the highest percentage of osteopenia was the neck bone (71.4%). The high percentage of osteopenia locations in the cervical spine of thalassemia major patients with repeated transfusions should be become a concern. Based on these findings, we held education and counselling on the risk of osteoporosis in thalassemia patients with repeated transfusions, osteoporosis exercise training and distribution of osteoporosis exercise compact disk (CD) for at home osteoporosis exercise training.
Cigarette Smoke Exposure, but Not High Fat Diet, is Able to Induce Atherosclerosis in Wild-Type Rats Qorry Amanda; Nyoman Suci Widyastiti; Yan Wisnu Prajoko; Nani Maharani; Udin Bahrudin
Journal of Biomedicine and Translational Research Vol 8, No 3 (2022): December 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i3.15744

Abstract

Background: High-fat diet (HFD) and cigarette smoke exposure (CSE) have been used to induce atherosclerosis in wild-type (WT) rats however, their effectivity remains unclear.Objective: To confirm and compare the effectivity of HFD and CSE on both the histopathology of aorta and the level of malondialdehyde (MDA) in WT rats.Methods: Thirty-six WT Wistar rats were divided into four main groups (K0, K1, K2, and K3) and four subgroups (K3A, K3B, K3C, and K3D). The negative control group (K0) was fed with regular diet. Group K1 was treated with an intravenous adrenaline followed by high-fat diet (HFD), K2 was fed with regular diet and given CSE, while K3 was treated with a combination of CSE and HFD. The serum and cardiac MDA levels were measured using ELISA. Hematoxylin eosin and oil red O staining of aorta were done for measuring of the intima-media thickness (IMT) ratio and for counting of foam cells, respectively. Results: Both serum and cardiac tissue MDA levels in either K1, K2, or K3 were significant higher (p<0.01) than that of in K0. IMT ratio in K3 was significant higher compared to other groups (p<0.01). Foam cell numbers were significant higher in K2 and K3 groups than that of in either K0 or K1 (p<0.01).Conclusion: While the HFD fails to induce atherosclerosis in WT rats for 28 days, either CSE or combination of CSE and HFD is able to induce it, and the combination is better than alone. 
Pengaruh pemberian ekstrak bekatul beras hitam (Oryza sativa L. indica) terhadap kadar MDA, SOD dan trigliserida pada tikus diabetes mellitus tipe 2 Monikasari Monikasari; Nyoman Suci Widyastiti; Endang Mahati; Ahmad Syauqy; Ahmad Ni’Matullah Al-Baarri
AcTion: Aceh Nutrition Journal Vol 8, No 1 (2023): March
Publisher : Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/action.v8i1.731

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In type 2 diabetes mellitus, the pancreas can produce the hormone insulin, but the body has difficulty using it. The study aimed to prove the effect of black rice bran extract on MDA, SOD, and triglyceride in type 2 Diabetic Mellitus rats. The method is quasi-experimental with a pre-posttest randomized control group design in 2021. The intervention was given for 21 days to 42 male Wistar rats. MDA examination using the TBARs method, SOD examination using a UV-Visible spectrophotometer. Triglyceride examination using a spectrophotometer. Statistical analysis used Paired T-Test or Wilcoxon test and One-Way ANOVA or Kruskal Wallis test. Descriptive research findings show that the intervention of black rice bran extract at a dose of 60 mg/200g reduced MDA in rats to 2,21 mmol/ml and triglycerides to 85,17 mg/dL. In addition, it can increase SOD to 69,67 units/ml. Almost the same as the intervention of metformin 9 mg/200 g body weight. However, statistically, no significant difference exists in the interventions carried out (p> 0,05). The study concluded a decrease in MDA, SOD, and triglyceride in rats, given the black rice bran extract intervention.
Renoprotective Effect Of Sambiloto (Andrographis Paniculata) Leaf Extract On Lipopolysaccharide – Induced Septic Rats Adhika Bastian Bagas Prananta; Nyoman Suci Widyastiti; Ariosta Ariosta; Dwi Retnoningrum; Rezya Salsabela; Vega Karlowee; Neni Susilaningsih
Journal of Biomedicine and Translational Research Vol 9, No 1 (2023): April 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i1.17286

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Background: Associated with sepsis is the illness known as acute kidney injury (AKI). AKI may be mediated by uric acid, and blood creatinine levels can be utilized to diagnose the condition to measure kidney function. Sambiloto (Andrographis paniculata) is a traditional medicine that has flavonoid compounds that can reduce creatinine levels and Xanthine Oxidase Inhibitors which can reduce uric acid levels.Objective: Septic model rats generated by lipopolysaccharide were used to test the effects of sambiloto (Andrographis paniculata) leaf extract on serum creatinine and uric acid levels (LPS).Methods: This study was experimental employing 25 rats split into 5 groups as the post-test alone control group: healthy control with standard feed (HC), negative control with LPS injection(NC), Treatment (T)1 (A.Paniculata 200 mg/kgBW+LPS), T2(A.paniculata 400 mg/kgBW+LPS), and T3(A.paniculata 500mg/kgBW+ LPS). A.paniculata leaf extract was given via oral gavage on day 8-21. Intraperitoneal injection of LPS 5 mg/kgBW was given on day 22. On the 25th day, the blood serum was analyzed for creatinine levels using Jaffe method and uric acid was analyzed using the enzymatic photometric method. One-way analysis of variance (Kruskal-Wallis) and the Kruskal-Wallis test were used to evaluate the data.Results: The mean creatinine levels of HC,NC,T1,T2,T3 were 0.7±0.01;3.5±0.04;2.9±0.03;1.9±0.05; 1.3 ±0.04 mg/dl respectively. The mean uric acid levels of HC.NC.T1.T2.T3 were 1.7±0.05;8.2±0.11; 4.5±0.03;4.0±0.12;3.0±0.19 mg/dl respectively. There were significant differences (p<0.05) in creatinine levels in groups T2(p=0.031) and T3(p=0.001) to NC group and serum uric acid levels in groups T1(p<0.001), T2(p<0.001), and T3(p<0.001) to NC group which creatinine and uric acid levels were lower than NC group.Conclusion: Andrographis paniculata leaf extract has renoprotective effect against AKI in LPS-induced septic ratsKeywords: Andrographis paniculata, creatinine, uric acid, lipopolysaccharide, AKI, sepsis
Effects of Andrographis paniculata Leaf Extract on C-Reactive Protein and Serum Ferritin in Lipopolysaccharide-Induced Sepsis Model Rat Rezya Salsabela; Nyoman Suci Widyastiti; Dwi Retnoningrum; Ariosta Ariosta; Neni Susilaningsih
Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal) Vol 12, No 3 (2023): JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL)
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dmj.v12i3.37645

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Background: C-reactive protein (CRP) and ferritin are acute-phase reactants that have demonstrated association with the severity of a variety of inflammation-mediated illnesses, including infection and sepsis. Sambiloto (Andrographis paniculata) leaf is traditionally used as an anti-inflammation and anti-bacterial herb. Sambiloto leaf extract possesses anti-inflammatory, antiallergenic, immuno-stimulatory, antiviral, and antioxidant activitiesObjective: To evaluate the effect of Sambiloto leaf extract (Andrographis paniculata) on inflammation biomarker (CRP and serum ferritin) in a rat model of sepsis-induced by lipopolysaccharide (LPS).Methods: This research was a true experimental study with a post-test-only control group design. Twenty five male Wistar rats were randomly divided into 5 groups consisting of a healthy control (HC), Negative control (NC), Treatment 1 (T1) Sambiloto 200mg/kgBW, T2 Sambiloto 400mg/kgBW, T3 Sambiloto 500mg/kgBW). Sambiloto leaf extract was given on days 8-21 and LPS 5mg/kgBW was injected intraperitoneal on day 22. On the 25th day, blood was drawn and  CRP and serum ferritin levels were analyzed using ELISA method. The data were analyzed using One-Way ANOVA and Kruskal Wallis test.Results: The mean CRP levels of HC, NC, T1, T2,T3 were 7.72 ± 2.53; 8.12 ± 1.08; 5.22 ± 1.71; 6.40 ± 1.19; 5.14 ± 1.37 ng/dl respectively. The mean serum ferritin level of HC, NC, T1, T2, T3 were 293.60 ± 66.53; 601.20 ± 100.17; 433.40 ± 194.65; 331.60 ± 75.97; 318.00 ± 69.64 ng/dl respectively. There were differences in CRP levels in groups T1(p=0.012) and T3(p=0.010), serum ferritin levels in groups T2(p=0.014) and T3(p=0.008) to the control group.Conclusion: Sambiloto (Andrographis paniculata) leaf extract  has anti-inflammatory effects in the rat model of sepsis.
The benefits of black rice bran and the potential of its bioactive compounds as antidiabetic agents Rafika Eviana; Nyoman Suci Widyastiti; Endang Mahati
AcTion: Aceh Nutrition Journal Vol 8, No 2 (2023): June
Publisher : Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/action.v8i2.907

Abstract

Prevention strategies for diabetes have been found to minimize the risk of the disease through lifestyle treatments such as physical exercise and nutrition. Plants with strong antioxidant activity have previously been discovered to be effective therapy for Type 2 Diabetes Mellitus. A total of 12 English articles were reviewed. Black rice bran, in its raw extract form, modulate the activity of enzymes, hormones, and molecular signaling related to glucose homeostasis in the body. Black rice bran increases the defense of pancreatic β cells against apoptosis. Bioactive substances in black rice bran such as anthocyanins, proanthocyanidins, γ-oryzanol regulate diabetes-related molecular signaling which ultimately leads to improvement in glucose levels, insulin levels, restoration and protection of pancreatic β cells against apoptosis. These bioactive substances are also possible in suppressing oxidative stress, increasing antioxidant status and decreasing inflammation which is a common condition in diabetes. The conclusion of this literature review was black rice bran has the potential to have an antidiabetic effect through various mechanisms, in which this effect is possible due to the bioactive substances contained in it.
Correlation between Ferritin Levels with Malondialdehyde and Neutrophil Lymphocyte Ratio on Iron Overload Imam Budiwiyono; Purwanto AP; Nyoman Suci Widyastiti; Hadian Hadian; Kusmiyati DK
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 27 No. 2 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i2.1675

Abstract

Thalassemia major is one of the types of thalassemia that need a routine blood transfusion. If not treated immediately, the patient will only last for 1-8 months. Blood transfusions performed at least or more than 10 times causes iron overload. Excess levels of Fe ions in the body will be stored in the form of ferritin. If the ferritin level is high, cell damage will occur in the presence of a fat peroxidation reaction or Malondialdehyde (MDA). Cell damage can trigger proinflammation, which increases neutrophil counts and decreases lymphocyte counts. The Neutrophil-Lymphocyte Ratio (NLR), which measures the ratio between Absolute Neutrophil Count (ANC) divided by Absolute Lymphocyte Count (ALC) with a manual peripheral blood picture. This study aimed to determine the correlation of ferritin levels with MDA and NLR in iron overload. This study used an analytical observational design with a cross-sectional approach, with samples were thalassemia patients who received repeated blood transfusions at the General Hospital Dr. R Soetrasno, Rembang City and Regional General Hospital Dr. R Soedjati, Grobogan Purwodadi. Inclusion criteria were age 10-18 years, transfusion 10-20 times, normal body temperature. Exclusion criteria were Fe therapy orally, leukocytosis, chronic kidney disease. In the MDA levels, there was no significant difference where p=0.25 by Spearman test. In the NLR there was no significant difference where p=0.91 by Spearman test. There is no correlation between ferritin levels with MDA and NLR in iron overload.
PERBEDAAN NEUTROPHIL-LYMPHOCYTE RATIO PADA SUBJEK BUKAN PEROKOK, PEROKOK RINGAN DAN PEROKOK SEDANG-BERAT Galang Bela Nusa; Nyoman Suci Widyastiti
Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal) Vol 5, No 4 (2016): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.159 KB) | DOI: 10.14710/dmj.v5i4.14451

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Latar Belakang : Merokok merupakan problem kesehatan serius yang menyebabkan angka kesakitan dan kematian yang tinggi. Paparan asap rokok yang berlangsung lama dapat memicu inflamasi pada saluran nafas dan parenkim paru perokok serta mempengaruhi jumlah dan hitung jenis leukosit. White Blood Cell Count (WBC) menggambarkan status inflamasi. Neutrofil dan limfosit merupakan bagian terbesar dari leukosit sehingga mampu menggambarkan sebagian besar respon imun tubuh. Neutrophil-Lymphocyte Ratio (NLR) dapat menggambarkan status inflamasi pada perokok.Tujuan : Membuktikan adanya perbedaan NLR antara subjek bukan perokok, perokok ringan dan perokok sedang-berat.Metode : Penelitian deskriptif analitik dengan pendekatan belah lintang dengan subjek penelitian sebanyak 36 orang yang dibagi menjadi tiga kelompok menurut klasifikasi perokok Sitepoe, yaitu kelompok subjek bukan perokok, perokok ringan dan perokok sedang berat. NLR didapat dengan membandingkan jumlah neutrofil absolut dengan jumlah limfosit absolut. Uji statistik menggunakan uji one way Anova dan uji Post Hoc Bonferroni.Hasil : Rerata NLR pada kelompok subjek bukan perokok sebesar 2,42 ± 0,51, kelompok subjek perokok ringan sebesar 3,01 ± 1,29 dan kelompok subjek perokok sedang-berat sebesar 2,02 ± 0,63. Uji Post Hoc menunjukkan ada perbedaan antara nilai NLR kelompok subjek perokok ringan dan perokok sedang berat (p=0,030) dan tidak ada perbedaan antara nilai NLR kelompok subjek bukan perokok dengan kedua kelompok lainnya (p=0,348; p=0,821).Kesimpulan : Terdapat perbedaan antara nilai NLR subjek perokok ringan dan perokok sedang-berat, namun tidak ada perbedaan antara nilai NLR antara subjek bukan perokok dengan perokok ringan maupun perokok sedang-berat.
Increased Levels of IFN-γ, PAI-1, and NT-proBNP are Associated with the Occurrence of Hypoxemia in COVID-19 Fathur Nur Kholis; Nur Farhanah; Charles Limantoro; Nyoman Suci Widyastiti; Mochamad Ali Sobirin
The Indonesian Biomedical Journal Vol 15, No 5 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i5.2457

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BACKGROUND: Acute respiratory distress syndrome (ARDS) is one of the severe complications of Coronavirus disease 2019 (COVID-19) that can lead to the occurrence of hypoxemia. Hypoxemia occurs due to the role of pro-inflammatory cytokines and coagulation factors. Several studies have shown that interferon (IFN)-γ, plasminogen activator inhibitor 1 (PAI-1) and N-terminal pro-B-type natriuretic peptide (NT-proBNP) are biological markers that can be used to evaluate the severity and prognosis of the disease in severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) infection. This study was conducted to evaluate the association between IFN-γ, PAI-1, NT-proBNP and hypoxemia in COVID-19 patients.METHODS: This was a cross-sectional study of COVID-19 subjects with hypoxemia. Hypoxemia assessment was performed based on arterial blood gas analysis. IFN-γ and PAI-1 were measured with ELISA, while NT-proBNP levels were measured with Roche NT-proBNP.RESULTS: Fifty-five COVID-19 subjects with hypoxemia were observed. Thirty subjects experiencing moderate to severe hypoxemia and 25 with mild hypoxemia. Levels of IFN-γ, PAI-1, and NT-proBNP were higher in COVID-19 subjects with moderate to severe hypoxemia compared to those with mild hypoxemia (261.14 (121-348.60) pg/mL vs. 145.50 (59.90-348.60) pg/mL, p<0.001; 5.47 (3.50-8.50) pg/mL vs. 3.40 (2.20-9.30) pg/mL, p<0.001; 760 (112-34,066) pg/mL vs. 71 (48-364) pg/mL, p<0.001).CONCLUSION: Elevated levels of IFN-γ, PAI-1, and NT-proBNP are associated with hypoxemia in COVID-19 patients, suggesting that these markers may be useful in assessing hypoxemia in COVID-19 patients.KEYWORDS: IFN-γ, PAI-1, NT-proBNP, hypoxemia, COVID-19
Effects of fiber and resistant starch in "Mangpis" cookies on changes in blood lipid profile in obese patients obesity Masdayani Junita Napitupulu; Ahmad Syauqy; Nyoman Suci Widyastiti; Diana Nur Afifah; Etika Ratna Noer
AcTion: Aceh Nutrition Journal Vol 9, No 1 (2024): March
Publisher : Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/action.v9i1.1269

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Abnormal lipid profiles can cause obesity-associated heart diseases. Increased total cholesterol, Low-Density Lipoprotein (LDL), triglyceride (TG), and High-Density Lipoprotein (HDL) levels are indicators of obesity. This study aimed to identify the dietary fiber and resistant starch in mangpis cookies and analyze the effects of mangpis cookies on reducing lipid profiles. This study used a pre-test and post-test  design to measure lipid profiles before and after eating mangpis. Mangpis cookies were prepared without wheat flour and replaced with 50% Bruguiera gymnorhiza mangrove flour and 50% banana flour. This experiment was conducted on 16 obese subjects. The sampling technique used was purposive. A decrease in the lipid profile was observed based on the results of blood tests before and after the consumption of mangpis cookies. The effect of the consumption of mangpis cookies on reducing lipid profiles was assessed using the Paired T-test and Wilcoxon test. The analysis results of the food fiber content and resistant starch contained in the mangpis cookies were 7,86 ± 004 and 6,65 ± 0,01 with p-value TG (p=0,001 and HDL (p=0,01). In conclusion, mangpis cookies were identified to contain 17,8% dietary fiber and 15% resistant starch, which could influence the lipid profile, especially TG levels.
Co-Authors Abd. Rasyid Syamsuri Adhika Bastian Bagas Prananta Adieba Warda Hayya Adriyan Pramono Afriana, Rizky Dyas Agus Fitrianto Ahmad Ni’Matullah Al-Baarri Ahmad Syauqy Ahmad Syauqy Ainun Najib Amelia, Sesa Anies Anies Ardhea Jaludamascena Ariosta Ariosta Ariosta Ariosta Ariosta Ariosta Ariosta Ariosta Ariosta Ariosta Arulita Ika Fibriana Astika Widy Utomo, Astika Widy Azizah, Arfianty Nur Banundari Rachmawati Brian Eka Putera Budhi Setianto Charles Limantoro Dea Amanda Caressa Devi Ermawati Dian Novita Diana Nur Afifah, Diana Nur Djuara P Lubis Dwi Retnoningrum Dwi Retnoningrum Dwi Retnoningrum Dyah Mustika Nugraheni Edi Dharmana Edi Dharmana Edward Kurnia Setiawan Edward Kurnia Setiawan Limijadi Edward Kurnia Setiawan Limijadi Ekowati, Liana Elida Soviana Elsa Naviati Endang Mahati Endang Mahati Endy Cahyono Kristiawan Erna Mirani Etika Ratna Noer Etika Ratna Noer Etika Ratna Noer Fathur Nur Kholis Fathur Nur Kholis Fathur Nur Kholis Galang Bela Nusa Hadian Hadian Hardhono Susanto Hardhono Susanto Henna Rya Abdurachmin Hertanto Wahyu Subagio I Edward Kurnia Setiawan L I Made Miarta Yasa Ima Arum Lestarini Imam Budiwiyono Indranila Kustarini Samsuria K. Heri Nugroho HS, K. Heri Kurniawan, Muhammad Farhan Kusmiyati DK Kusmiyati Tjahjono Mabruratussania Maherdika Mahalul Azam Maria Mexitalia Martha Irene Kartasurya Masdayani Junita Napitupulu Meira Erawati Meita Hendrianingtyas Meita Hendrianingtyas Meita Hendrianingtyas Michael Kevin Mochamad Ali Sobirin Moedrik Tamam Moedrik Tamam Monikasari Monikasari Muji Rahayu Mulya Agustina Mulyono Mulyono Muniroh, Muflihatul Nahwa Arkhaesi Najma Khairani Harahap Nani Maharani Naufal, Haidar Satya Neni Susilaningsih Neni Susilaningsih Neni Susilaningsih Ninik Rustanti Novianti, Anugrah Nugroho, Trilaksana Nur Farhanah Nur Farhanah Partiwi, Rina Purwanto Adipireno Purwanto Adipireno Purwanto AP Qorry Amanda R. Lindeman Rafika Eviana Ratih Budinastiti Retnoningrum, Dwi Rezya Salsabela Rezya Salsabela Rina Pratiwi Roziana Roziana, Roziana Rr. Sri Ratna Rahayu Ruth Hanna Kristina Sabar Widodo Salma Tyas Salsabila Saputra, Taufik Selamat Budijitno Selamat Budijitno Setianingrum, Elisabeth Levina Sari Silitonga, Marisi Elizabeth R. Suhartono Suhartono, Suhartono Susanti Lestari Thijs Eijsvogels Titis Yoga Safitri Tri Indah Winarni Udin Bahrudin Udin Bahrudin Umi S Intansari Vega Karlowee Widyastuti, Trioni Yan Wisnu Prajoko Yan Wisnu Prajoko Yanuarita Tursinawati Yanuarius Alvin Pratama Budianto Yetty Movieta Nancy Yetty Movieta Nency Yetty Movieta Nency Yetty Movieta Nency Yetty Movieta Nency, Yetty Movieta Yuliana Yuliana Zetta, Adelia Paradya