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All Journal HAYATI Journal of Biosciences Jurnal Kesehatan Reproduksi Biogenesis: Jurnal Ilmiah Biologi Jurnal Kesehatan Andalas Mutiara Medika: Jurnal Kedokteran dan Kesehatan Jurnal Teknologi Laboratorium BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan BIOEDUSCIENCE Jurnal Biologi Tropis SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Jurnal Pengabdian Masyarakat AbdiMas JSFK (Jurnal Sains Farmasi & Klinis) Jurnal Ilmu Kesehatan Masyarakat (The Public Health Science Journal) 2-TRIK: TUNAS-TUNAS RISET KESEHATAN Health Sciences and Pharmacy Journal Health and Medical Journal SULUH: Jurnal Abdimas Abdimas UNWAHAS : Jurnal Pengabdian Masyarakat UNWAHAS BERNAS: Jurnal Pengabdian Kepada Masyarakat Bioscientist : Jurnal Ilmiah Biologi PUINOVAKESMAS Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal Kesehatan Reproduksi SEGARA : Jurnal Pengabdian Kepada Masyarakat Jurnal Kedokteran Meditek Jurnal Ilmu Kesehatan Masyarakat (The Public Health Science Journal)
Meiskha Bahar
Universitas Pembangunan Nasional Veteran Jakarta
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan)

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EFFECT OF FERMENTATION OPTIMIZATION WITH PH CONTROL ON ACTINOMYCETES ISOLATE AS ANTIBACTERIAL STAPHYLOCOCCUS AUREUS Joanne, Anastasia; Bahar, Meiskha; Muktamiroh, Hikmah; Setyaningsih, Yuni
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 10 No. 2 (2024): February
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v10i2.10529

Abstract

Actinomycetes are Gram-positive bacilli noted for their secondary metabolites that are effective as antibacterials. Production of the antibacterial compounds is determined by nutrition, temperature, pH, and fermentation time. This study aims to study the antibacterial activity of Actinomycetes isolates from Bogor Botanical Gardens soil by fermentation optimization with pH control on the 3rd, 4th, and 5th day against the growth of Staphylococcus aureus bacteria. Actinomycetes isolates were inoculated on Starch Casein Agar (SCA) and fermented in liquid media containing 7 g glucose, 14 g mannitol, 14 g peptone for 5 days with the pH controlled within the range of 6.2–6.8. Antibacterial test method against S.aureus using well diffusion method with Nutrient Agar (NA) media. The inhibition zone diameter was measured every 24 hours and and the results obtained were 3.73 mm; 6.05mm; and 5.34mm. Those data were analyzed using the Kruskal-Wallis test and then continued with the Mann Whitney test which had significant differences between groups. The conclusion of the study is Actinomycetes isolates have antibacterial potential against the growth of S. aureus with the largest average diameter of inhibition zone in the 4th day fermentation group, which had moderate inhibition.
Co-Authors Abdul Kolib Agneta Irmarahayu Ahmad Fauzantoro Andre Fahriz Perdana Harahap Andri Pramesyanti Andri Pramesyanti Pramono Anisa Sri Mulyani Anisah Anisah Annisa Nur Insani Aulia Chairani Aulia Chairani, Aulia Basra Ahmad Amru Cut Fauziah Cut Fauziah Cut Fauziah Cut Fauziah Cut Fauziah Cut Fauziah Cut Fauziah Erna Harfiani Fadel Abima Fadel Abima, Fadel Fajriati Zulfa Fajriati Zulfa Halimah Anggi Rahmani Hany Yusmaini Insani, Annisa Nur Joanne, Anastasia Kori Yati Mahdi Jufri Makkiyah, Feda Anisah Maria Selvester Thadeus Melly Kristanti Misri Gozan Mitta Nurfitri Saridewi Monica, Astried Muhammad Yusuf Arya Ramadhan Muhammad Zaidan Tsani Ariandi Muharany, Raden Roro Ariesna Mulyani, Anisa Sri Nasihin Saud Irsyad Nunuk Nugrohowati Nurfitri Bustamam Oktania Sandra Oktania Sandra Puspita Oktavia, Sabina Pasiak, Taufiq Fredrik Pertiwi Sudomo Pramesyanti, Andri Prananda, Faadhilah Widya Purwaningastuti, Diana Agustini Putrialif, Hayatunufus Retno Yulianti Rizki Putri Andini Rahmah Saskia Arientika Wahyuningrum Sembiring, Rinawati Setyaningsih, Yuni Sheila Azelya Fernanda Sri Wahyuningsih Sudomo, Pertiwi Sukrisno, Adi Taufiq Fredrik Pasiak Taufiq Fredrik Pasiak Tri Faranita Triandini, Erdieny Fahliza Ufi Dewintera Utomo, Muhammad Gifta Venita Octavia Tambunan Wahyuningrum, Saskia Arientika Wibowo, Naufal Rafi Kusuma Yanti Harjono Hadiwiardjo Yudhi Nugraha Yudhi Nugraha Zulfa , Fajriati