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All Journal HAYATI Journal of Biosciences Jurnal Kesehatan Reproduksi Biogenesis: Jurnal Ilmiah Biologi Jurnal Kesehatan Andalas Mutiara Medika: Jurnal Kedokteran dan Kesehatan Jurnal Teknologi Laboratorium BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan BIOEDUSCIENCE Jurnal Biologi Tropis SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Jurnal Pengabdian Masyarakat AbdiMas JSFK (Jurnal Sains Farmasi & Klinis) Jurnal Ilmu Kesehatan Masyarakat (The Public Health Science Journal) 2-TRIK: TUNAS-TUNAS RISET KESEHATAN Health Sciences and Pharmacy Journal Health and Medical Journal SULUH: Jurnal Abdimas Abdimas UNWAHAS : Jurnal Pengabdian Masyarakat UNWAHAS BERNAS: Jurnal Pengabdian Kepada Masyarakat Bioscientist : Jurnal Ilmiah Biologi PUINOVAKESMAS Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal Kesehatan Reproduksi SEGARA : Jurnal Pengabdian Kepada Masyarakat Jurnal Kedokteran Meditek Jurnal Ilmu Kesehatan Masyarakat (The Public Health Science Journal)
Meiskha Bahar
Universitas Pembangunan Nasional Veteran Jakarta
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Journal : Jurnal Kesehatan Andalas

 1 
Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro Saskia Arientika Wahyuningrum; Meiskha Bahar; Andri Pramesyanti Pramono
Jurnal Kesehatan Andalas Vol 10, No 1 (2021): Online March 2021
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v10i1.1595

Abstract

Pneumonia is a lung parenchymal infection caused by Pseudomonas aeruginosa.It is Gram negative bacteria that have developed antibiotic resistance. Actinomycetes are Gram-positive bacteria that produce secondary metabolites which have the ability as antimicrobial. Objectives: To identified the ability of Actinomycetes isolates to inhibit the growth of the bacterium Pseudomonas aeruginosa ATCC 27853. The samples in this experiment were from Kebun Raya Bogor that had been rejuvenated on Starch Casein Agar (SCA). Methods: Six dilution series 10-1; 10-2; 10-3; 10-4; 10-5; 10-6 Actinomycetes isolates were used to observe the inhibition zone of P.aeruginosa growth on Mueller Hinton Agar (MHA) media by diffusion method. Results: The effective incubation time occurred at 24 hours, and then it resulted in the average clear zone diameter of 14.70 mm, 10.57 mm, 8.53 mm, 8.47 mm, 6.97 mm, and 5.30 mm. The results of the One – Way Anova test with p-value = 0.000 (p < 0.005) showed some differences at each concentration to inhibit the growth of P.aeruginosa ATCC 27853 at 24 hours incubation period. Conclusion: The most effective concentration of Actinomycetes isolates that can potentially be antibacterial was the concentration of 10-1 with potential solid inhibitory power.Keywords: Actinomycetes, antibacterial, Pseudomonas aeruginosa
Aktivitas Daya Hambat Isolat Actinomycetes dengan Lama Fermentasi yang Berbeda terhadap Pertumbuhan Klebsiella pneumoniae Annisa Nur Insani; Meiskha Bahar; Nunuk Nugrohowati; Retno Yulianti
Jurnal Kesehatan Andalas Vol 11, No 2 (2022): Online July 2022
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v11i2.2014

Abstract

Actinomycetes are filamentous, Gram-positive bacteria widely distributed in soil and can produce secondary metabolites useful as antibiotics. The synthesis of secondary metabolites of Actinomycetes is influenced by the growth conditions of each isolate, including the fermentation period. Klebsiella pneumoniae is an opportunistic bacterial pathogen causing lower respiratory tract infection. Objectives: To determined the inhibition activity of Actinomycetes with different fermentation periods against the growth of  K.pneumoniae. Methods: Actinomycetes were isolated from soil in Kebun Raya Bogor and grown on Starch Casein Agar (SCA) medium before being fermented in liquid media consisting of 2% of mannitol, 2% of peptone, and 1% of glucose with an incubation time of 6,7, and 8 days. An antibacterial activity test was conducted on Mueller Hinton Agar (MHA) medium using the well diffusion method. Results: The activity of Actinomycetes isolates with fermentation duration of 6,7 and 8 were able to inhibit the growth of K. pneumoniae with an average inhibition zone of 4,46 mm; 4,94 mm; and 5,04 mm, respectively. Actinomycetes' inhibitory mechanism ranges from inhibition of cell wall synthesis to nucleic acid and protein synthesis. One Way ANOVA test showed significant differences between the inhibition zone produced by each fermentation group. Conclusion: Actinomycetes isolates have antibacterial activity against the growth of K. pneumoniae with the highest activity achieved by Actinomycetes with a fermentation time of 8 days.Keywords:  Actinomycetes, antibacterial, fermentation, Klebsiella pneumoniae
Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro Wahyuningrum, Saskia Arientika; Bahar, Meiskha; Pramono, Andri Pramesyanti
Jurnal Kesehatan Andalas Vol. 10 No. 1 (2021): Online March 2021
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v10i1.1595

Abstract

Pneumonia is a lung parenchymal infection caused by Pseudomonas aeruginosa.It is Gram negative bacteria that have developed antibiotic resistance. Actinomycetes are Gram-positive bacteria that produce secondary metabolites which have the ability as antimicrobial. Objectives: To identified the ability of Actinomycetes isolates to inhibit the growth of the bacterium Pseudomonas aeruginosa ATCC 27853. The samples in this experiment were from Kebun Raya Bogor that had been rejuvenated on Starch Casein Agar (SCA). Methods: Six dilution series 10-1; 10-2; 10-3; 10-4; 10-5; 10-6 Actinomycetes isolates were used to observe the inhibition zone of P.aeruginosa growth on Mueller Hinton Agar (MHA) media by diffusion method. Results: The effective incubation time occurred at 24 hours, and then it resulted in the average clear zone diameter of 14.70 mm, 10.57 mm, 8.53 mm, 8.47 mm, 6.97 mm, and 5.30 mm. The results of the One – Way Anova test with p-value = 0.000 (p < 0.005) showed some differences at each concentration to inhibit the growth of P.aeruginosa ATCC 27853 at 24 hours incubation period. Conclusion: The most effective concentration of Actinomycetes isolates that can potentially be antibacterial was the concentration of 10-1 with potential solid inhibitory power.Keywords: Actinomycetes, antibacterial, Pseudomonas aeruginosa
Aktivitas Daya Hambat Isolat Actinomycetes dengan Lama Fermentasi yang Berbeda terhadap Pertumbuhan Klebsiella pneumoniae Insani, Annisa Nur; Bahar, Meiskha; Nugrohowati, Nunuk; Yulianti, Retno
Jurnal Kesehatan Andalas Vol. 11 No. 2 (2022): Online July 2022
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v11i2.2014

Abstract

Actinomycetes are filamentous, Gram-positive bacteria widely distributed in soil and can produce secondary metabolites useful as antibiotics. The synthesis of secondary metabolites of Actinomycetes is influenced by the growth conditions of each isolate, including the fermentation period. Klebsiella pneumoniae is an opportunistic bacterial pathogen causing lower respiratory tract infection. Objectives: To determined the inhibition activity of Actinomycetes with different fermentation periods against the growth of  K.pneumoniae. Methods: Actinomycetes were isolated from soil in Kebun Raya Bogor and grown on Starch Casein Agar (SCA) medium before being fermented in liquid media consisting of 2% of mannitol, 2% of peptone, and 1% of glucose with an incubation time of 6,7, and 8 days. An antibacterial activity test was conducted on Mueller Hinton Agar (MHA) medium using the well diffusion method. Results: The activity of Actinomycetes isolates with fermentation duration of 6,7 and 8 were able to inhibit the growth of K. pneumoniae with an average inhibition zone of 4,46 mm; 4,94 mm; and 5,04 mm, respectively. Actinomycetes' inhibitory mechanism ranges from inhibition of cell wall synthesis to nucleic acid and protein synthesis. One Way ANOVA test showed significant differences between the inhibition zone produced by each fermentation group. Conclusion: Actinomycetes isolates have antibacterial activity against the growth of K. pneumoniae with the highest activity achieved by Actinomycetes with a fermentation time of 8 days.Keywords:  Actinomycetes, antibacterial, fermentation, Klebsiella pneumoniae
Co-Authors Abdul Kolib Agneta Irmarahayu Ahmad Fauzantoro Andre Fahriz Perdana Harahap Andri Pramesyanti Andri Pramesyanti Pramono Anisa Sri Mulyani Anisah Anisah Annisa Nur Insani Aulia Chairani Aulia Chairani, Aulia Basra Ahmad Amru Cut Fauziah Cut Fauziah Cut Fauziah Cut Fauziah Cut Fauziah Cut Fauziah Cut Fauziah Erna Harfiani Fadel Abima Fadel Abima, Fadel Fajriati Zulfa Fajriati Zulfa Halimah Anggi Rahmani Hany Yusmaini Insani, Annisa Nur Joanne, Anastasia Kori Yati Mahdi Jufri Makkiyah, Feda Anisah Maria Selvester Thadeus Melly Kristanti Misri Gozan Mitta Nurfitri Saridewi Monica, Astried Muhammad Yusuf Arya Ramadhan Muhammad Zaidan Tsani Ariandi Muharany, Raden Roro Ariesna Mulyani, Anisa Sri Nasihin Saud Irsyad Nunuk Nugrohowati Nurfitri Bustamam Oktania Sandra Oktania Sandra Puspita Oktavia, Sabina Pasiak, Taufiq Fredrik Pertiwi Sudomo Pramesyanti, Andri Prananda, Faadhilah Widya Purwaningastuti, Diana Agustini Putrialif, Hayatunufus Retno Yulianti Rizki Putri Andini Rahmah Saskia Arientika Wahyuningrum Sembiring, Rinawati Setyaningsih, Yuni Sheila Azelya Fernanda Sri Wahyuningsih Sudomo, Pertiwi Sukrisno, Adi Taufiq Fredrik Pasiak Taufiq Fredrik Pasiak Tri Faranita Triandini, Erdieny Fahliza Ufi Dewintera Utomo, Muhammad Gifta Venita Octavia Tambunan Wahyuningrum, Saskia Arientika Wibowo, Naufal Rafi Kusuma Yanti Harjono Hadiwiardjo Yudhi Nugraha Yudhi Nugraha Zulfa , Fajriati