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All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis JURNAL KIMIA SAINS DAN APLIKASI Jurnal Natur Indonesia Berkala Perikanan Terubuk Biogenesis: Jurnal Ilmiah Biologi Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Jurnal Ilmiah Pertanian Chimica et Natura Acta Akta Kimia Indonesia JURNAL PHOTON Unri Conference Series: Community Engagement Narra J International Journal of Basic and Applied Science PENA ABDIMAS : Jurnal Pengabdian Masyarakat Dinamika Pertanian Jurnal Natur Indonesia
Titania Tjandrawati Nugroho
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Journal : JURNAL KIMIA SAINS DAN APLIKASI

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Isolation of a High Antioxidant Non-Toxic Polar Fraction from Garcinia mangostana Fruit Pericarp by Reverse Phase Column Chromatography Wahyuningsih Wahyuningsih; Miranti Miranti; Hilwan Yuda Teruna; Titania Tjandrawati Nugroho
Jurnal Kimia Sains dan Aplikasi Vol 24, No 1 (2021): Volume 24 Issue 1 Year 2021
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3020.51 KB) | DOI: 10.14710/jksa.24.1.15-21

Abstract

The crude polar extract of mangosteen fruit pericarp not only has a moderate antioxidant activity of (55±4 μg/mL) but also has high cytotoxicity (16±0.5 μg/mL). The high cytotoxicity presumably is caused by the presence of complex cytotoxic compounds from the mangosteen pericarp. To obtain a non-toxic extract preparation with high antioxidant activity, polar crude 50% ethanol extracts of mangosteen pericarp were partially purified using reverse-phase column chromatography with Silica C18 as the stationary phase and acetonitrile-water gradient elution. Six of the ten fractions collected had high antioxidant activities, with IC50 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging antioxidant levels <50 μg/mL. Three fractions (fractions 3, 5, and 7) with the highest antioxidant activities of (16.4 ± 0.6 µg/mL), (17.8 ± 2 µg/mL) and (17.4 ± 1.8 µg/mL) respectively, were chosen for further cytotoxicity, phenolic content and High-Performance Liquid Chromatography (HPLC) analysis. The cytotoxic tests were conducted with the Brine Shrimp Lethality Assay. Fraction 3 had low cytotoxicity (LC50 485 ± 96 µg/mL) and fraction 5 was non-toxic (LC50 ≥ 1000 µg/mL), while fraction 7 still had high cytotoxicity (LC50 2.8 ± 0.8 µg/mL). The chromatogram profiles of HPLC showed that fractions 3 and 5 contained more polar compounds than the compounds present in fraction 7. It can be concluded that the reverse phase method succeeded in the isolation of a non-toxic polar fraction, that is, fraction 5, with a significantly higher (p<0.05) antioxidant activity than in the original crude polar extracts. This fraction had a high total phenolic content of 43.3 ± 0.3 g GAE per 100 g extract.
Microbial Growth as Determinant of Antibiotic Production with Biotic Elicitors Stimulation Citra Hardiyanti; Khairullinas Khairullinas; Jeky Sasemar Lumban; Titania Tjandrawati Nugroho; Yuana Nurulita
Jurnal Kimia Sains dan Aplikasi Vol 23, No 3 (2020): Volume 23 Issue 3 Year 2020
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2803.553 KB) | DOI: 10.14710/jksa.23.3.89-95

Abstract

An antibiotic-resistant and multidrug-resistant (MDR) issue open the role of researchers to continue to search for natural potential as a source of new antimicrobials. One of the potential fungi isolates that can produce antimicrobial active compounds from Indonesian tropical peat soils is Penicillium sp. LBKURCC34. In this study, the production of antimicrobial compounds from local isolates was carried out by batch fermentation method in liquid media with the addition of biotic elicitors to increase the extraction activity and yield. This study aims to optimize the results based on the time the elicitor is added. Staphylococcus aureus was used as a biotic elicitor, which was added on days 2, 3, and 4 in the production of antibiotics by fermentation incubation of 6-14 days. The antibiotic production media was extracted with ethyl acetate and evaporated. The antimicrobial test was carried out by the disk diffusion method against pathogenic bacteria Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Staphylococcus epidermidis using three crude extract contents (19; 38; and 57 µg/disc). Amoxicillin® was used as a positive control (10 µL/disc). The results showed that the addition of S. aureus biotic elicitor extended the log phase growth of the fungus Penicillium sp. LBKURCC34. The optimum condition of production was obtained by adding initiator treatment on the 3rd day for 14 days incubation with the highest yield and could inhibit the growth of all pathogenic microbes.
Co-Authors - Miranti Adja Muhammad Alsan Shaf Andhra Syatapahana Andi Dahliaty Andi Dahliaty Andi Dahliaty Annisa Fitri Ayu Octa Mellani Bustari Hasan Citra Hardiyanti Desmelati Desmelati Dian Iriani Dian Novita Sary Edy Fachrial Feliatra Fifi Puspita Fifi Puspita Fifira Safitri Fitri Rahayu Fitri, Nur Annisa Gaol, Fuji Randa Lumban Gurning, Esther Angelita Halida Sophia Harni Sepriani Hilwan Yuda Teruna Iin Evita Sari Ismawati Ismawati Jeky Sasemar Lumban Khairullinas Khairullinas M Andesba Arifin Miranti Miranti Miranti Miranti Miranti Miranti Miranti, - Narasswati, Nungki Nenci, Nuryani Nova Rianti Putri Novia Sellyna Nur Azlina Nuria Wulandari Nurwijayanti Nuryani Nenci Oktavia, Rani Puja, Krisna Puspita Sari Putri Nabilah Putri, Nova Rianti Ramadhan, Gilang Rani Yuniati Safitri, Selfi Yanti Sakinah, Alifia Andra Saputra, Rachmad Saryono Saryono &#039; Saryono Saryono Saryono, M.Or. S.Pd. Jas. Senjavi Rakhmana Sepriani, Harni Seprianto, Seprianto Sila Silalahi Siregar, Siti Saidah Siregar, Siti Saidah Sri Helianty suraya, nabella Susilawati - Susilawati, Susilawati Utama, Panca Setia Wahyuningsih Wahyuningsih Yanti Lim Yanti Yanti Yanti Yanti Yuana Nuralita Yuana Nurulita Yuana Nurulita Yuharmen - Yuharmen Yuharmen Yum Eryanti Yuslina Octavia Zona Octarya Zona Octarya