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All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis JURNAL KIMIA SAINS DAN APLIKASI Jurnal Natur Indonesia Berkala Perikanan Terubuk Biogenesis: Jurnal Ilmiah Biologi Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Jurnal Ilmiah Pertanian Chimica et Natura Acta Akta Kimia Indonesia JURNAL PHOTON Unri Conference Series: Community Engagement Narra J International Journal of Basic and Applied Science PENA ABDIMAS : Jurnal Pengabdian Masyarakat Jurnal Farmasi Indonesia Dinamika Pertanian Jurnal Natur Indonesia
Titania Tjandrawati Nugroho
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Journal : berkala perikanan terubuk

 1 
Aktivitas Enzim Protease dan Lipase Viscera Ikan Kembung (Restrelliger sp) pada pH Dan Konsentrasi Garam Berbeda Bustari Hasan; Desmelati Desmelati; Dian Iriani; Titania Nugroho; M Andesba Arifin; Andhra Syatapahana
Berkala Perikanan Terubuk Vol 43, No 2 (2015): Juli 2015
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.734 KB) | DOI: 10.31258/terubuk.43.2.68 - 76

Abstract

This research was conducted to measure the activity of visceral protease and lipase of mackerel (Rastrelligersp) at different pH and salt concentration. Mackerel, weighing ± 150 g/each were obtained from a fish market in Pekanbaru. Fish weretransported to the laboratory; and at the laboratory, the fish were eviscerated and their visceral organs (intestines, kidneys, liver, spleen, cord, pancreas and gonad) were blended in Tris-HCl buffer and filtered for their crude enzyme extract. The protease activity was measured at pH 6, 7, 8, 9, 10 and 11; and lipase activity was measured at pH2, 3, 4, 5, 5.5, 6, 7, 8, 9, 10 and 11. The protease and lipase activity werealso measured atsalt concentration 0%, 3%, 6%, 9%, 12% and 15%. The results showed that the protease and lipase activity varies with pH value and salt concentration. Protease activity at pH 11 (1,610µmol/ml)and salt concentration 6%(0,869 µmol/ml)showed the highest activity; and pH 10 (0,031µmol/ml) and salt concentration 15% (0,073µmol/ml) was the lowest activity. Lipase activity at pH 4 (2,428 µmol/ml) and salt concentration 3% (4,131µmol/ml) was the highest activity; and pH 11 (0,042µmol/ml) and salt concentration 15% (2,237µmol/ml) was the lowest activity. The protease and lipase activity were decreased withthe increasing of salt concentration.
Co-Authors - Miranti Adja Muhammad Alsan Shaf Andhra Syatapahana Andi Dahliaty Andi Dahliaty Andi Dahliaty Annisa Fitri Ayu Octa Mellani Bustari Hasan Citra Hardiyanti Desmelati Desmelati Dian Iriani Dian Novita Sary Edy Fachrial Feliatra Fifi Puspita Fifi Puspita Fifira Safitri Fitri Rahayu Fitri, Nur Annisa Gaol, Fuji Randa Lumban Gurning, Esther Angelita Halida Sophia Harni Sepriani Hendra, Rudi Hilwan Yuda Teruna Iin Evita Sari Ismawati Ismawati Jasril Jasril Jeky Sasemar Lumban Khairullinas Khairullinas Lestari, Retno Puji M Almurdani M Andesba Arifin Miranti Miranti Miranti Miranti Miranti Miranti Miranti, - Narasswati, Nungki Nenci, Nuryani Nova Rianti Putri Novia Sellyna Nur Azlina Nuria Wulandari Nurwijayanti Nuryani Nenci Oktavia, Rani Puja, Krisna Puspita Sari Putri Nabilah Putri, Nova Rianti Ramadhan, Gilang Rani Yuniati Rizky Abdulah Safitri, Selfi Yanti Sakinah, Alifia Andra Saputra, Rachmad Saryono Saryono ' Saryono Saryono Saryono, M.Or. S.Pd. Jas. Senjavi Rakhmana Sepriani, Harni Seprianto, Seprianto Sila Silalahi Siregar, Siti Saidah Siregar, Siti Saidah Sri Helianty suraya, nabella Susilawati - Susilawati, Susilawati Utama, Panca Setia Wahyuningsih Wahyuningsih Yanti Lim Yanti Yanti Yanti Yanti Yuana Nuralita Yuana Nurulita Yuana Nurulita Yuharmen - Yuharmen Yuharmen Yum Eryanti Yuslina Octavia Zona Octarya Zona Octarya