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All Journal HAYATI Journal of Biosciences Indonesian Journal of Tropical and Infectious Disease Oceana Biomedicina Journal Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL Indian Journal of Forensic Medicine & Toxicology Griya Journal of Mathematics Education and Application Narra J Folia Medica Indonesiana
Usman Hadi
Dept. Of Internal Medicine School Of Medicine Airlangga University/Dr. Soetomo Hospital
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Education Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health

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Molecular Genotyping of Tsst-1 Gene Staphylococcus Aureus from Clinical Specimen Puspitarini, Marinda Dwi; Setyarini, Wahyu; Hadi, Usman; Kuntaman, Kuntaman
Folia Medica Indonesiana Vol. 57, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

Staphylococcus aureus (S. aureus) is the most common pathogen in humans that causes various infections. Variations in infections due to S. aureus are related to the presence of virulence factors, one of which is Toxic Shock Syndrome Toxin-1 (TSST-1) which is the main cause of Toxic Shock Syndrome (TSS). This study aimed to explore the genetic pattern of the tsst-1 genes among MSSA and MRSA from clinical isolates of patients at Dr. Soetomo Academic Hospital, Surabaya-Indonesia. PCR examination was performed on 106 clinical samples of S. aureus isolates to determine the presence of the tsst-1 genes; subsequently, phylogenetic analysis was performed. The positive tsst-1 genes were found in 3 (5.7%) MSSA isolates and 2 (3.8%) MRSA isolates from 106 samples, of which were divided into 53 MSSA isolates and 53 MRSA isolates. No significant difference was noticed between the tsst-1 genes on both the MSSA and MRSA bacteria (p = 0.648). The positive tsst-1 genes were found in 1 (4.5%) of pus specimens and 2 (16.7%) of blood specimens from MSSA isolates. However, the positive tsst-1 genes were found in neither sputum nor urine specimens. The genes were found in 2 (16.7%) of blood specimens from MRSA isolates. Based on the phylogenetic tree, Indonesian tsst-1 isolates belonged to the same clade as Japan, Iran, Iraq, India, Egypt and the United Kingdom isolates. The prevalence of tsst-1 genes of both MSSA and MRSA from clinical isolates in Dr. Soetomo Academic Hospital Surabaya are 5.7% and 3.8%, respectively. The tsst-1 genes of Surabaya-Indonesia, Japan, Iran, Iraq, India, Egypt and the United Kingdom belonged to the same clade.
Correlation of Population and Environmental Behavior with Rat Density Rate in Plague Disease-Focus, Threatened and Safe Areas in Tutur District, Pasuruan Regency, 2016 Lestari, Evi Noerista; Andajani, Susilowati; Hadi, Usman
Folia Medica Indonesiana Vol. 55, No. 4
Publisher : Folia Medica Indonesiana

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Plague disease remains a public health problem in the world. According to the International Health Regulations (IHR), an outbreak is an infectious disease that has the potential to cause an outbreak. The purpose of this study was to analyze a significant relationship between human behavior and the environment with the density of the number of rats in the focus, threatened and outbreak-safe areas in Tutur Regency, Pasuruan Regency, in 2016. This was an observational analytic study with cross correlation sectional where the research variables are population behavior, environment, and the number of rat densities observed once at a time. The results showed that there was no significant relationship between the behavior (knowledge, attitude, and practice) of the population with the density of rats in the focus area of the Surorowo hamlet outbreak, in the endangered outbreaks of the Ngaro hamlet, and in the outbreak-safe area of the North Ngandong hamlet in Tutur District. Whereas, there is a significant relationship between environmental conditions and the amount of rat density in the focus area of the outbreak of the Surorowo hamlet (p: 0.047 or p
Analysis of Quality of Antibiotic Usage on Patient with Internal and Surgical Service Ma'rifah, Nurul; Hasmono, Didik; Hadi, Usman; Kuntaman, Kuntaman
Folia Medica Indonesiana Vol. 57, No. 1
Publisher : Folia Medica Indonesiana

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It is well-known that hospitals are health facility with the widely use of antibiotics. It is about 13-37% from the total hospitalized patients in developed countries use antibiotic, even in developing countries can reach 30-80%. There is identified correlation between antibiotic use and the development of bacterial resistance. Even though the resistance cannot be eliminated, but its development can be suppressed by the increasing of prudent use of antibiotics. The aim of this study was to determine the quality of antibiotic use on internal medicine and surgical patients in Aisiyah Hospital Bojonegoro. The study was a prospective cross sectional observational analytical study of among patients of internal and surgical who received antibiotic therapy in the period of August - September 2017. The total 50 samples were collected in this study which consists of 33 internal medicine and 17 surgical patients. From 50 samples, there were 16 types of antibiotics with the total use of 81 of antibiotic use. As the result, in internal medicine patients there were 22 (40%) of appropriate use of antibiotics, 4 (7.27%) of inappropriate use and 29 (52.73%) use of antibiotics without indication. In surgical patients, there were 12 (46.15%) of appropriate use of antibiotics, 2 (7.69%) of inappropriate use and 12 (46.15%) use of antibiotics without indication. This study showed that more than 50% of antibiotic use were inappropriate, and mainly antibiotic with no indication, among patients hospitalized in Aisyiyah Hospital Bojonegoro.
Co-Authors Agung Dwi Wahyu Widodo Agus Santosa Agus Santosa Agustinus Vincent Arfijanto, Muhammad V. Arfijanto, Vitanata Aryati , Aryati Aryati Aryati Aryati Aryati Baehaqi Benediktus Yohan Bramantono Bramantono, Bramantono Didik Hasmono Djazuly Chalidyanto Eddy Soewandojo Eko Budi Koendhori, Eko Budi Erni J. Nelwan, Erni J. Erni Juwita Nelwan, Erni Juwita Erwin Astha Triyono Florentina Sustini Gondo Mastutik Hardiono Hardiono, Hardiono Hari Paraton Haryo N. Yahya Hidajat, Boerhan Insani Budiningsih Iqhrammullah, Muhammad Ismi Masyithah Jaap M. Middeldorp Juanita, Lydia Kuntaman Kuntaman Laila Hayati, Laila Lestari, Evi Noerista Ma'rifah, Nurul Margalin, Brilliant Margyaningsih, Nur Ita Marinda Dwi Puspitarini Mariyatul Qiptiyah, Mariyatul Masyeni, Sri Muchlis AU Sofro Muhammad Miftahussurur Muhammad Vitanata Arifijanto Nenci Siagian Nurul Ma'rifah P. Fans Pathak, Yashwant Prihartini Prihartini Purnomo, Windu Puspitarini, Marinda Dwi R. Tedjo Sasmono Ronald Pratama Adiwinoto Sasmono, R. Tedjo Setyarini, Wahyu Sholeh, Mohammad Akbaruddin Sofro, Muchlis AU Sofro, Muchlis AU. Sri Masyeni, Dewa Ayu Putri Sripatmi Sripatmi, Sripatmi Suci Andriani Sugiyatmi, Tri A. Suharto Suharto Susilowati Andajani, Susilowati Wahyu Setyarini Windu Purnomo Yoes Prijatna Dachlan Yohan, Benediktus