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All Journal HAYATI Journal of Biosciences Bioscience Jurnal Tumbuhan Obat Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Buletin Penelitian Tanaman Rempah dan Obat Sains: Jurnal Manajemen dan Bisnis Pharmauho: Jurnal Farmasi, Sains, dan Kesehatan Jurnal SOLMA JFIOnline Buletin Penelitian Tanaman Rempah dan Obat Sainstech Farma: Jurnal Ilmu Kefarmasian Jurnal Pengabdian Masyarakat Indonesia Jurnal Bina Pengabdian Kepada Masyarakat Jurnal Farmasi Etam Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Indonesian Journal of Cancer SAINSTECH: Jurnal Penelitian dan Pengkajian Sains dan Teknologi Mestaka: Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Masyarakat Bangsa GEMBIRA (Pengabdian Kepada Masyarakat) PEDAMAS (Pengabdian Kepada Masyarakat) Borneo Journal of Pharmacy JCSE: Journal of Community Service and Empowerment Masyarakat: Jurnal Pengabdian Jurnal Kesehatan Masa Depan
Febriani, Amelia
Institut Sains Dan Teknologi Nasional
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Journal : Bioscience

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Isolation and characterization of lactic acid bacteria in fermented sweet corn (Zea mays l) as antibacterial Rosario Trijuliamos Manalu; Amelia Febriani; S. Syamsinar
Bioscience Vol 5, No 2 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202152113708-0-00

Abstract

Sweet corn (Zea mays L.) is a staple food that is known to produce Lactic Acid Bacteria (LAB) during the fermentation process, LAB is a probiotic bacterium that can produce antimicrobial components. This study aims to examine the characteristics and antibacterial activity of LAB sweet corn against Escherchia coli and Staphylococcus aureus.The study was conducted by isolating LAB from corn fermentation spontaneously which then tested the characteristics of the isolates obtained and tested for antibacterial activity. From the isolation results 3 isolates were obtained, namely FJM 1, FJM 2 and FJM 3 with morphological characteristics of rod-shaped and Gram-positive cells and biochemical test results; negative catalase, citrate, indole, motility, VP and positive TSIA. The results of antibacterial activity tests on E. coli were obtained respectively at 14.18 mm, 12.38 mm and 11.18 mm and S. aureus respectively at 13.05 mm, 11.12 mm and 10.35 mm. All three isolates had strong category antibacterial activity. Jagung manis (Zea mays L.) adalah makanan pokok yang diketahui menghasilkan Bakteri Asam Laktat (BAL) selama proses fermentasi, BAL merupakan suatu bakteri probiotik yang dapat menghasilkan komponen antimikroba. Penelitian ini bertujuan untuk menguji karakteristik dan aktivitas antibakteri BAL jagung manis terhadap Escherchia coli dan Staphylococcus aureus. Penelitian dilakukan dengan mengisolasi BAL dari fermentasi jagung secara spontan yang kemudian diuji karakteristik dari isolat yang didapatkan serta diuji aktivitas antibakterinya. Dari hasil isolasi didapatkan 3 isolat yaitu FJM 1, FJM 2 dan FJM 3 dengan karakteristik morfologi sel berbentuk batang dan Gram positif serta hasil uji biokimia; katalase, sitrat, indol, motilitas, Vp negatif dan TSIA positif. Hasil uji aktivitas antibakteri terhadap E. coli didapatkan berturut sebesar 14,18 mm, 12,38 mm dan 11,18 mm dan S. aureus berturut sebesar 13,05 mm, 11,12 mm dan 10,35 mm. Ketiga isolat memiliki aktivitas antibakteri kategori kuat.
Co-Authors Ainun Wulandai Ainun Wulandari Ajeng Wahyuningrum Akhatik, Nurul Amaniah, Naila Ananda Digita Andini, Issri Mila Nova Arum, Fitria Diah Asishe Asishe Aulia Rahimi Berna Elya Chori Azizah Dea Selia Dede Heri Yuli Yanto Dede Heri Yuli Yanto Deni Zulfiana Deni Zulfiana Desy Muliana Wenas Desy Satrana Devi Purwita Sari Dini Andiani Dini Andiani, Dini Dini Juliana Diny Auliya Mawarni Dwi Okta Mellya Erna Yanti Erwi Putri Setyaningsih Eva Oktavia Fathin Hamida Firdamayanti, Firdamayanti Fitri Sulistyaningsih Fitria Rahayu Fitria Yustisiani Herdini Herdini , Herdini Hervianti Nurfitria Nugrahani Hery Syahrial Husna, Fadia Hutagaol, Sartika Ika Maruya Ika Maruya Kusuma Jaya, Rendy Indra Jerry, Jerry Jessica Khairunnisa Krisnawati, Indah Kusuma Hidayatullah Kusuma, Ika Maruya Lidia Anggita Ramadhani Lili Musnelina Maghfirullah, Achmad Ilham Mahdi Jufri Manalu, Mia Aty Dolok Rosa Marsa Falihah Maulida Oktaviani Mega Hariyani Melva Novriana Saragih Muh. Faris Hidayat Mulandita Munawarohthus Sholikha Munawarohthus Sholikha Munawarohthus Sholikha Munawarohthus Sholikhah Muzallifah, Muzallifah Nafisah Nuha Nopianti, Hermila Novita, Windi Nugrahani, Hervianti Nurfitria Nurfitri Nurfitri Nurhasanah, Sari Nurul Akhatik Oktan Dwi Nurhayat Pontoan, Jenny Pratiwi Ally Florentina Pujiastuti, Fitria Putri , Elvina Triana Putri, Elvina Triana Rachmatiah, Tiah Rachmayanti Dewi Rahmi Syariifatul Ratna Nilam Cahya Refdanita Refdanita Refdanita Risma Eka Putri Ritha Widyapratiwi Rogahang, Preisilia Rohmawati, Fitri Rosario Trijuliamos Manalu Rosario Trijuliamos Manalu S. Syamsinar Saiful Bahri Saiful Bahri Setiadi, Fandy Rezha Shella Utami Sholikha, Munnawarohthus Sister Sianturi Siswati Siswati Sita Heris Anita Sita Heris Anita Siti Fadila Azahra Siti Koriah, Siti Siti Nurmiati Subaryanti Subaryanti, Subaryanti Suci Asriatul Nirmala Suci Tiara Suryadi Suryadi Suyatno Suyatno Syafriana, Vilya Tarigan, Eka Dewi Setia Taufik Hidayat Teodhora, Teodhora Teti Indrawati Tiah Rachmatiah Tri Soehartati Veriyanti , Putu Rika Veryanti, Putu Rika Welisa, Selfina Junita Widyapratiwi, Ritha Winahayu, Neng Esti Winnugroho Wiratman, Manfaluthy Hakim, Tiara Aninditha, Aru W. Sudoyo, Joedo Prihartono Yayah Siti Djuhariah Yunisa Simehete Zabitha Zahwa Zahra, Nada