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Fakhrurrazi Fakhrurrazi
Fakultas Kedokteran Hewan, Universitas Syiah Kuala
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Jurnal Medika Veterinaria

 1 
12. Isolate Of Bacteriua Cellulase Enzyme Production In The Rumen Of Aceh Cattle Base On Analysis Homology 16S rRNA Indah Melzana; Safika Safika; Darmawi Darmawi; Erina Erina; Fakhrurrazi Fakhrurrazi; Al Azhar
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.3415

Abstract

The rumen of cattle is well known one of a bacterium cellulolitic production that have ability to degrade cellulose for produce cellulase enzymes. The aims of the present study is to determine the type of bacteria produced cellulase enzymes in the rumen of cattle aceh. The diversity of cellulolitic bacteria is determined by  analysis of 16S rRNA genes. Isolation of cellulolytic bacteria, total DNA isolation, agarose gel electrophoresis, amplification of the 16S rRNA gene, determining the DNA sequence, DNA sequence homology and alignment of DNA sequences. Isolation of bacterium cellulolitic, isolating total DNA, gel electroforesis agarosa, amplified of gen 16S rRNA, determination the DNA barcode,  homology DNA barcode, and alignment of DNA sequences. The sample of rumen liquid was taked from RPH , then maked isolation of total DNA to get pure DNA from bacterium whos result and amplification DNA of bacterium cellulolitic separated by electroforesis gel agarosa. Gene of bacterium cellulolitic was isolated based Polymerase Chain Reaction methode. Alignment is done by using BlastN program, then use CLustalW program.  It can be concluded that the species of bacteria (FKH_USK_6) in rumen has a closeness with Coronabacter sakazakii bacteria strain PNP8 of 97% according to the sizeof the DNA sequence and the same position on an existing gene in GenBank.
TOTAL BAKTERI PSIKOTROPIK IKAN NILA (Oreochromis niloticus) YANG DIBERI PENINGKATAN SUHU PADA SAAT PEMELIHARAAN (Total of Phsycotrophic Bacteria of Nile Tilapia (Oreochromis niloticus) Reared in High Water Temperature) Safni Kamal; Nurliana Nurliana; Faisal Jamin; Sulasmi Sulasmi; Hamny Hamny; Fakhrurrazi Fakhrurrazi
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v10i1.4035

Abstract

This research aimed to calculate the number of phsycotrophic bacteria of nile tilapia reared in increased water temperature. For this purpose, 12 nile tilapias with body weight of 35-40 g were randomly allotted into 2 treatments groups with 3 replications. Fish in group 1 (P1) was reared in normal temperature (29±1° C) and group II (P2) was reared in high water temperature (35±1 C). All fish were fed commercial diet 3 times a day for 21 consecutive days. T-test analysis revealed a significant decrease in number of psychotropic bacteria (P0.01) in group P2. Total plate count (TPC) resulted in fish gills, intestinal, and muscle were 1.5×10³, 7.7x10³, and 9.7x10³ cfu/g respectively. In conclusion, increasing of water temperature reduce the number of phsycotrophic bacteria of nile tilapia.
IDENTIFIKASI BAKTERI Staphylococcus aureus PADA PREPUTIUM DAN VAGINA KUDA (Equus caballus) (Identification of Staphylococcus aureus in Preputium and Vagina of Horses (Equus caballus)) Yulina Rahmi; Darmawi Darmawi; Mahdi Abrar; Faisal Jamin; Fakhrurrazi Fakhrurrazi; Yudha Fahrimal
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3805

Abstract

This research aims to identify the Staphylococcus aureus (S. aureus) bacteria from preputium and vagina of horses. Five swabs preputium and 5 swabs vagina samples from 3-9 years olds horse in the village of Bebangka, Gelelungi, Reloop, and Kelapeng, Pegasing Aceh Tengah District were used in this study. Preputium and vagina swabs of horses were dipped into reaction tube containing Nutrient Broth (NB). Staphylococcus aureus was identified on manitol salt agar (MSA). Separated colony colored bright yellow on the media were tested for Gram staining, hemolysis, catalase, and sugar test (glucose and manitol). The result showed that the isolates growth on MSA were colored bright yellow, Gram positive, β hemolisis, and catalase positive. The isolates fermented manitol and glucose. Isolates identified from horses preputium and vagina showed the characteristic of S. aureus. In conclusion S. aureus bacteria were positively identified from all of swabs samples, both in preputium and vagina of horses.Key words: Staphylococcus aureus, horse, preputium, vagina
12. Antibacterial Activity of Ethanol Extract of Siamih Leaf (Ageratum conyzoides) on Staphylococcus aureus bacteria divina dinda hayati; Herrialfian Herrialfian; M. Isa; Darmawi Darmawi; Fakhrurrazi Fakhrurrazi; Abdul Harris
Jurnal Medika Veterinaria Vol 14, No 1 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i1.10333

Abstract

The aim of this research is to know the effect of siamih leaf ethanol extract (Ageratum conyzoides) on the growth of Staphylococcus aureus bacteria. The bacteria which is  used are obtained from the Microbiology Laboratory of the Faculty of Veterinary Medicine of Syiah Kuala University on isolate bacteria of the honey bear wound (Helarctos malayanus). Bacterial identification is done by culturing the isolate on lateral NA media then it is planted on MSA media and  staining Gram, catalase test and hemolysis test. The extraction is done by maceration with 96% ethanol solvent. Extract of Siamih leaf  is done by phytochemical test. The results of pythritic test of Siamih leaf show that Siamih leaf contains alkaloid compounds, steroids, terpenoids, flavonoids and tannins. The extract concentration which is used is 20%, 40%, 60% and 80%. Nergative control by using aquades and positive controls using Amoxicillin with three repetitions. The method used is disc diffusion or Kirby-Bauer method. The results show that Siamih leaf ethanol extract inhibit the growth of Staphylococcus aureus bacteria at concentrations of 20% (0,7 mm), 40% (1,4 mm), 60% (2,2 mm), and 80 % (3,0 mm). Thus it can be concluded that the extract of siamih leaf ethanol has inhibitory power to Staphylococcus aureus bacteria with low category.
2. Anti-Bacterial Activity Of N-Hexane Extract Of Malacca Leave (Phyllanthus emblica) On Mice (Mus musculus) Inoculated By Staphylococcus epidermidis In Vivo Nada Sarah Syahputri; Nuzul Asmilia; Rinidar Rinidar; Amalia Sutriana; Fakhrurrazi Fakhrurrazi; Ginta Riady
Jurnal Medika Veterinaria Vol 15, No 1 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i1.19840

Abstract

Malacca plant (Phyllanthus emblica) is one of the medicinal plants. The purpose of this study was to determine the effect of n-hexane extract of Malacca (Phyllanthus emblica) leaves on the growth of Staphylococcus epidermidis bacteria in vivo. All mice were first induced by Staphylococcus epidermidis bacteria. Negative control (K1) was given aquadest, positive control (K2) was given ciproflaxacin suspension at doses of 20 mg/kg BW, while K3, K4, and K5 were given n-hexane extract of Malacca leave at dose of 100 mg/kg BW, 200 mg/kg BW, and 300 mg/kg BW. Respectively blood sampling was carried out on the 5th day after treatment. Data were analyzed using one-way analysis of variance (ANOVA). The results showed that the mean (± SD) number of bacterial colonies in K1 was 656x10² cfu/ml. The average number of bacterial colonies in K2 was 2328x10² cfu/ml. The average number of bacterial colonies given n-hexane extract of malacca leave 100 mg/kg BW on K3 was 359,60x10² cfu/ml. The average number of bacterial colonies given n-hexane extract of malacca leave 200 mg/kg BW at K4 was 200x10² cfu/ml and the average number of bacterial colonies given n-hexane extract of malacca leave 300 mg/kg BW at K5 was 3483x10² cfu/ml. The results showed there were no significant difference among treatment groups (P 0.05). N-hexane extract of malacca leave was unable to inhibit the growth of Staphylococcus epidermidis bacteria in vivo
ISOLASI BAKTERI Salmonella sp. PADA FESES ANAK AYAM BROILER DI PASAR ULEE KARENG BANDA ACEH (Isolation of Salmonella sp. in Feces of Broiler Chicks at Ulee Kareng Market Banda Aceh) Afriyani Afriyani; Darmawi Darmawi; Fakhrurrazi Fakhrurrazi; Zakiah Heryawati Manaf; Mahdi Abrar; Winaruddin Winaruddin
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v10i1.4047

Abstract

The research aimed to isolate Salmonella sp.in feces of broiler chicks. Fifteen feces samples of broiler chick were obtained from Ulee Kareng Market in Banda Aceh. Chick cloaca was swabbed using a sterile cotton swab, put into the microtube, and brought to the Microbiology Laboratory of Veterinary Medicine Faculty, Syiah Kuala University. The samples were incubated in selenite cystine broth (SCB) medium, cultured in salmonella shigella agar (SSA) and separated colonies were inoculated in nutrien agar (NA) at 37° C for 24 hours. Bacteria stained with Gram staining then tested biochemically. The result showed that Salmonella sp. were identified in 9 out of 15 feces chicks samples. It ca be concluded that broiler chick in Ulee Kareng Market Banda Aceh contaminated with bacteria Salmonella sp.
4. Case Study: Gallstones (Cholelithiasis) in Long-tailed Macaques (Macaca fascicularis) J. Susilo; B. Tri Wibowo; Y. Tinasari; M. Nur Salim; Dian Masyitha; Erdiansyah Rahmi; M. Isa; Herialfian Herialfian; Fakhrurrazi Fakhrurrazi; T. Fadrial Karmil; Fadli A. Gani
Jurnal Medika Veterinaria Vol 15, No 2 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i2.25204

Abstract

The purpose of this case study is to diagnose the cause of death of the long-tailed macaque (Macaca fascicularis). Two cadaver of Macaca fascicularis were received by the Lampung Veterinary Center from a customer. Necropsy was carried out at the Pathology Laboratory of the Lampung Veterinary Center by observing macroscopic changes. The samples taken were then observed by gram staining in the Bacteriology laboratory and hematoxylin-eosin staining at the Pathology laboratory. The results of macroscopic observations showed an enlarged gallbladder with a length of 4 cm and a width of 2 cm, blackish in color. Gallbladder surgery found 15 gallstones measuring 2 mm in diameter, brownish-colored bile, fibrin clots, and blood. The native gram stain of bile showed several types of rod-shaped bacteria form and gram-negative cocci (eosinophilic), and gram-positive rods (basophilic). Specific histopathological changes in the ductus choleductus are multifocal neutrophil inflammatory cell infiltration, enlarged gallbladder lumen filled with multi-bacterial, cell debris, fibrin, and erythrocytes. Multifocal hepatitis occurs by neutrophils, multibacterial, and fibrin. It can be concluded that the causes of death of Macaca fascicularis were cholelithiasis and hepatitis.
8. The Role of Bovine Viral Diarrhea Virus In Bovine Respiratory Disease Complex In Cattle Import Livestock At 2019 Periode J Susilo; J Siswanto; T. Fadrial Karmil; Rusli Rusli; M. Nur Salim; Zainuddin Zainuddin; Ginta Riady; Dasrul Dasrul; Fakhrurrazi Fakhrurrazi; Azhari Azhari; Andi Novita
Jurnal Medika Veterinaria Vol 14, No 1 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i1.17402

Abstract

Bovine viral diarrhea virus (BVDV) is one of the most important infectious diseases in cattle breeding. Bovine respiratory disease complex (BRDC) is a respiratory disease in mature and calf cattle which is a viral and bacterial superinfection including BVDV. The purpose of reseach is to study the role of BVDV in cases of Bovine respiratory disease complex (BRDC), especially with the characteristic of lesions were granulomatous pneumonia. The material in this study was lung tissue that was fixed with 10% formaldehyde buffer of calf sampel collected in October and November 2019. The sample is processed in a pathology laboratory for hematoxyline eosin (HE) staining and followed by immunohistochemical staining (IHC). A total of 11 lung samples sent from imported beef cattle and dairy cattle with a diagnosis of pneumonia complex morphology continued with CPI testing with monoclonal antibodies against bovine viral diarrhea virus. Nine samples showed positive CPI results against BVDV and two other samples were negative. Bovine viral diarrhea virus is detected in all granulomatous pneumonia, so it has an important role in BRDC. Early detection, treatment and prevention of BRDC affected or followed by BVDV must be carried out programmatically.
Co-Authors . Darmawi . Darniati . Nurliana Abdul Harris Afriyani Afriyani Al Azhar Amalia Sutriana Andi Novita Azhari Azhari B. Tri Wibowo Cut Dahlia Iskandar Darmawi Darmawi Darmawi Darmawi Darmawi Darmawi Darniati Darniati Dasrul Dasrul Dian Masyitha divina dinda hayati Erdiansyah Rahmi Erina Erina Erina Erina Erina Erina Erina Erina Fadli A. Gani Faisal Jamin Faisal Jamin Ginta Riady Hamdani Budiman Hamny Sofyan Herialfian Herialfian Herrialfian . Indah Melzana J Siswanto J Susilo J. Susilo M Daud AK M Nur Salim M. Isa M. Isa M. Isa M. Jalaluddin Mahdi Abrar Mahdi Abrar Maryulia Dewi Maryulia Dewi Masda Admi Nada Sarah Syahputri Nucha Nabila Nur Nuzul Asmilia Rinidar Rinidar Rizki Kurniawan Kurniawan Roslizawaty Roslizawaty Rusli Rusli Safika S, Safika Safni Kamal Sugito Sugito Sulasmi Sulasmi T Zahrial Helmi T. Fadrial Karmil T. Fadrial Karmil T. Fadrial Karmil Winaruddin Winaruddin Y. Tinasari Yudha Fahrimal Yulina Rahmi Zainuddin Zainuddin Zakiah Heryawati Manaf Zakiyah Heryawati Manaf