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Aktivitas Antibiotik terhadap Biofilm Staphylococcus aureus Isolat Preputium Sapi Aceh (Antibiotic Activities To Staphylococcus aureus Biofilms Of Aceh Cattle Preputium Isolate) Maryulia Dewi; Darmawi Darmawi; Nurliana Nurliana; T. Fadrial Karmil; T Zahrial Helmi; Fakhrurrazi Fakhrurrazi; Erina Erina; Mahdi Abrar; M. Daud AK; Masda Admi
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.35219

AbstractBiofilm is one of parameters for determining pathogenic pathways of a bacterium. Bacterial virulence activities can be attenuated by antibiotics with inhibiting biofilm. The aim of this study was to examine the characteristics and activities of antibiotics against the biofilm of Stapylococcus aureus isolate which taken from Aceh cattle prepuce. The study was exploratory and experimental, using a Completely Randomized Design (RAL) factorial pattern using three types of antibiotics, three concentrations and replications. Antibiotics were tetracycline, oxytetracycline and phosphomycin with concentrations of 10%, 20% and 30% (mg/mL), respectively. Therefore, the aim of this study was to examine the characteristics and activities of antibiotics to the biofilm of Stapylococcus aureus from Aceh cattle prepuce isolate. U-bottom polystyrene 96 wells microplate and Nutrient Broth (NB) medium were used to biofilm. Bacterial while biofilm was stained on crystals violet 1%. Optical Density (OD) of biofilm were λ 595 nm. Data were analyzed by using Analysis Of Variance (ANOVA). The results showed that the activity of Staphylococcus aureus was moderately positive, i.e 2,667cell/μL. It showed that antibiotics and concetrations have significant effect (P<0.05) to inhibit biofilm staphylococcus aureus. In additioon, tetracycline 30% (mg / mL) has the best ability to inhibit biofilms from 52,18% to 1,262 cell/μL. Based on that fact, it can be concluded that the virulence of Staphylococcus aureus was moderate positive to be false positive. Keywords: biofilm, antibiotics, Staphylococcus aureus, cows aceh. Abstrak Biofilm merupakan salah satu parameter untuk menentukan jalur patogenisitas suatu bakteri. Antibiotik dapat melemahkan aktivitas virulensi bakteri dengan menghambat pembentukan biofilm. Penelitian ini bertujuan untuk melihat karaksteristik dan aktivitas antibiotik terhadap biofilm Stapylococcus aureus isolat preputium sapi Aceh. Penelitian bersifat eksplorasi dan eksperimental, menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan menggunakan tiga jenis antibiotik, tiga konsentrasi dan tiga kali ulangan. Antibiotik yang digunakan adalah tetrasiklin, oksitetrasiklin dan fosfomisin dengan konsentrasi masing-masing 10%, 20% dan 30% (mg/mL). Pengujian biofilm dilakukan pada media Nutrient Broth (NB), menggunakan mikroplat U-bottom polystyrene 96 wells. Pewarnaan biofilm menggunakan kristal violet 1%. Optical Density (OD) biofilm dibaca pada λ 595 nm. Data hasil penelitian dianalisis dengan menggunakan Analysis Of Variance (ANOVA). Hasil penelitian menunjukkan aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik biofilm tergolong positif moderat, yaitu 2,667cell/µL. Uji Biofilm menunjukkan jenis dan konsentrasi antibiotik berpengaruh secara nyata (P<0,05) terhadap daya hambat biofilm Staphylococcus aureus isolat preputium sapi Aceh. Tetrasiklin 30% (mg/mL) mempunyai kemampuan paling baik menghambat pembentukan biofilm Staphylococcus aureus isolat preputium sapi Aceh sebesar 52,18% menjadi 1,262 cell/µL, sehingga aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik biofilm dari positif moderat menjadi positif palsu. Kata Kunci: biofilm, antibiotik, Staphylococcus aureus, sapi aceh.

Isolasi Dan Identifikasi Bakteri Salmonella Sp. Pada Daging Ikan Kuniran (Upeneus Sulphureus) Di Pasar Lampulo Banda Aceh Nucha Nabila Nur; Fakhrurrazi Fakhrurrazi; Cut Dahlia Iskandar; Faisal Jamin; Roslizawaty Roslizawaty; Ginta Riady
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 4 (2022): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i4.21510

ABSTRAKIkan kuniran merupakan salah satu bahan makanan yang memiliki sumber protein tinggi, mengandung asam amino esensial yang diperlukan oleh tubuh. Kondisi pasar lampulo yang ramai dan kurangnya higienitas dari tempat penjualan ikan, merupakan penyebab ikan terkontaminasi oleh bakteri Salmonella. Penelitian ini bertujuan untuk mendeteksi adanya cemaran bakteri Salmonella sp. pada daging ikan kuniran (Upeneus sulphureus) di Pasar Lampulo Banda Aceh. Penelitian ini menggunakan metode Carter. Sampel yang digunakan dalam penelitian berjumlah 8 sampel yang diambil menggunakan metode observasional. Ikan kuniran diambil sebanyak 1 gram/sampel, lalu digerus dan ditanam pada media SCB (Salmonella Cystine Broth) dan diinkubasi dengan suhu 37oC selama 24 jam. Bakteri yang tumbuh ditanam pada media SSA (Salmonella Shigella Agar) dan diinkubasi dengan suhu 37oC selama 24 jam. Setelah itu dilakukan pewarnaan Gram dan penanaman bakteri pada media NA miring. Media NA miring diinkubasi pada suhu 37ºC selama 24 jam lalu dilakukan uji biokimia. Hasil penelitian dianalisis secara deskriptif dan data disajikan dalam bentuk gambar dan tabel. Dapat disimpulkan bahwa ikan kuniran positif tercemar oleh bakteri Salmonella sp. berdasarkan pemeriksaan mikrobiologi.ABSTRACTKuniran fish is one of the foodstuffs that has a high protein source, containing essential amino acids needed by the body. The crowded conditions of the Lampulo market and the lack of hygiene from fish selling places are the causes of fish being contaminated by Salmonella bacteria. This study aims to detect the presence of Salmonella sp. on the flesh of kuniran fish (Upeneus sulphureus) at the Lampulo Market, Banda Aceh. This study uses the Carter method. The sample used in the study amounted to 8 samples taken using the observational method. Kuniran fish were taken as much as 1 gram/sample, then planted on SCB (Salmonella Cystine Broth) media and incubated at 37oC for 24 hours. The bacteria that grew were grown on SSA (Salmonella Shigella Agar) media and incubated at 37oC for 24 hours. After that, Gram staining and bacteria were planted on slanted NA media. The inclined NA medium was incubated at 37ºC for 24 hours and then a biochemical test was performed. The results of the study were analyzed descriptively and the data was presented in the form of pictures and tables. It can be concluded that the positive kuniran fish contaminated by Salmonella sp. based on microbiological examination.

Invasif dan Persisten Pasteurella multocida Setelah Diinokulasi Secara Intratracheal pada Kalkun Mahdi Abrar; Fakhrurrazi Fakhrurrazi
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

The objective of this study was to evaluate the invasiveness and persistence of Pasteurella multocida after intratracheal inoculation on turkeys. Four strains of Pasteurella multocida, P-1059, T-325, CU, and M-9 were used in this study and intratracheally (IT) inoculated into turkeys. The number of viable bacteria in the trachea, lung, liver, spleen, and the blood were enumerated during periods of 6 and 9 hrs post inoculation (PI). The result showed that four strains of organism were present in the organ and the tissues observed. In the liver, spleen, and blood, the virulent P-1059 strain showed significant increases within 6 or 9 hrs PI. Strain CU showed significant increase only in the spleen. Two other strains did not show significant increase in any organs; strain M-9, on the other hand, showed significant decrease in the trachea. These results indicated that P-1059 and three other strains showed their ability to invade the tissues or organs when they are intratracheally inoculated.

Isolation and Characterization of Avian Influenza Virus Based on Macroscopic Examination of Native Chicken Embryo Fakhrurrazi Fakhrurrazi; Hamdani Budiman
Jurnal Kedokteran Hewan Vol 3, No 2 (2009): September
Publisher : Universitas Syiah Kuala

The aim of this research was to isolate and characterize the virus based on macroscopic examination of native chickens embryo infected with the AI virus. This research used 10 infected native chickens from Ateuk Pahlawan and Doi villages. All chickens were examined to identify the present of AI using rapid test and in ovo virus propagation. Rapid test showed 7 chickens from Ateuk Pahlawan villageand 5 chickens from Doi village were AI positive. Propagation of the viruses in 11 days old chicken embryo in ovo showed that embryo died 5 days post inoculated whereas macroscopic examination showed dwarfed embryo, hemorrhagic, and loss of feather.

Isolasi Dan Identifikasi Bakteri Eschericia Coli Pada Produk Ceker Ayam Bakar Di Gampong Ulee Lheue Kota Banda Aceh Rizki Kurniawan Kurniawan; Darniati Darniati; Mahdi Abrar; Fakhrurrazi Fakhrurrazi; M. Jalaluddin; Erina Erina
JURNAL ILMIAH MAHASISWA VETERINER Vol 7, No 2 (2023): FEBRUARI-APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v7i2.20644

ABSTRAKPenelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Eschericia coli yang mungkin mengontaminasi produk ceker ayam bakar di Gampong Ulee Lheue, Kecamatan Meuraxa, Kota Banda Aceh. Penelitian ini dilakukan berdasarkan metode Carter (1987) yang telah dimodifikasi. Sampel penelitian yang digunakan berjumlah 10 sampel dan diambil dari 5 pedagang yang berbeda. Sampel diisolasi pada media Nutrient Broth (NB) dan diinkubasikan selama 24 jam dengan suhu 37° C. Hasil positif pertumbuhan pada media NB kemudian diinokulasikan pada media Eosin Methylen Blue Agar (EMBA) untuk dilakukan pengamatan morfologi koloni bakteri secara makroskopis. Selanjutnya, dilakukan pewarnaan Gram terhadap koloni bakteri untuk pengamatan morfologi secara mikroskopis. Identifikasi bakteri Eschericia coli dilakukan melalui uji biokimia pada media Indol, Methyl Red-Voges Proskauer (MR-VP), Simmon’s Citrate Agar (SCA), Triple Sugar Iron Agar (TSIA), Sulfide Indol Motility (SIM), serta media gula-gula (glukosa, sukrosa, laktosa, maltosa, dan manitol). Data hasil penelitian ini dianalisis secara deskriptif dan ditampilkan dalam bentuk gambar dan tabel. Hasil penelitian menunjukkan 9 sampel (90%) positif terkontaminasi Eschericia coli yang disimpulkan bahwa produk ceker ayam bakar yang dijual di Gampong Ulee Lheue Kota Banda Aceh telah terkontaminasi oleh bakteri Eschericia coli.Kata kunci: Ceker ayam, Eschericia coli, Foodborne disease, Ulee LheueABSTRACTThis study aims to isolate and identify the possibility of Eschericia coli contamination on grilled chicken feet products in Gampong Ulee Lheue, Meuraxa District, Banda Aceh City. This research is based on the modified of Carter’s (1987) method. The research sampel used were 10 samples and were taken from 5 different traders. Samples were isolated on Nutrient Broth (NB) media and incubated for 24 hours at 37° C. The positive results of growth on NB media were then inoculated on Eosin Methylen Blue Agar (EMBA) media for macroscopic bacterial colonies morphological observations. Furthermore, Gram staining was performed on bacterial colonies for microscopic morphological observations. Identification of Eschericia coli conducted through biochemical tests on Indole, Methyl Red-Voges Proskauer (MR-VP), Simmon's Citrate Agar (SCA), Triple Sugar Iron Agar (TSIA), Sulfide Indol Motility (SIM), and sugars (glucose, sucrose, lactose, maltose, and mannitol). The data from this research were analyzed descriptively and displayed in the form of pictures and tables. The results showed that 9 samples (90%) were positively contaminated with Eschericia coli which concluded that grilled chicken feet products sold in Gampong Ulee Lheue, Banda Aceh City have been contaminated by Eschericia coli bacteria.Keyword: Chicken feet, Eschericia coli, Foodborne disease, Ulee Lheue

Invasif dan Persisten Pasteurella multocida Setelah Diinokulasi Secara Intratracheal pada Kalkun Mahdi Abrar; Fakhrurrazi Fakhrurrazi
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i2.9815

The objective of this study was to evaluate the invasiveness and persistence of Pasteurella multocida after intratracheal inoculation on turkeys. Four strains of Pasteurella multocida, P-1059, T-325, CU, and M-9 were used in this study and intratracheally (IT) inoculated into turkeys. The number of viable bacteria in the trachea, lung, liver, spleen, and the blood were enumerated during periods of 6 and 9 hrs post inoculation (PI). The result showed that four strains of organism were present in the organ and the tissues observed. In the liver, spleen, and blood, the virulent P-1059 strain showed significant increases within 6 or 9 hrs PI. Strain CU showed significant increase only in the spleen. Two other strains did not show significant increase in any organs; strain M-9, on the other hand, showed significant decrease in the trachea. These results indicated that P-1059 and three other strains showed their ability to invade the tissues or organs when they are intratracheally inoculated.

Isolation and Characterization of Avian Influenza Virus Based on Macroscopic Examination of Native Chicken Embryo Fakhrurrazi Fakhrurrazi; Hamdani Budiman
Jurnal Kedokteran Hewan Vol 3, No 2 (2009): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v3i2.9788

The aim of this research was to isolate and characterize the virus based on macroscopic examination of native chickens embryo infected with the AI virus. This research used 10 infected native chickens from Ateuk Pahlawan and Doi villages. All chickens were examined to identify the present of AI using rapid test and in ovo virus propagation. Rapid test showed 7 chickens from Ateuk Pahlawan villageand 5 chickens from Doi village were AI positive. Propagation of the viruses in 11 days old chicken embryo in ovo showed that embryo died 5 days post inoculated whereas macroscopic examination showed dwarfed embryo, hemorrhagic, and loss of feather.

12. Isolate Of Bacteriua Cellulase Enzyme Production In The Rumen Of Aceh Cattle Base On Analysis Homology 16S rRNA Indah Melzana; Safika Safika; Darmawi Darmawi; Erina Erina; Fakhrurrazi Fakhrurrazi; Al Azhar
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.3415

The rumen of cattle is well known one of a bacterium cellulolitic production that have ability to degrade cellulose for produce cellulase enzymes. The aims of the present study is to determine the type of bacteria produced cellulase enzymes in the rumen of cattle aceh. The diversity of cellulolitic bacteria is determined by analysis of 16S rRNA genes. Isolation of cellulolytic bacteria, total DNA isolation, agarose gel electrophoresis, amplification of the 16S rRNA gene, determining the DNA sequence, DNA sequence homology and alignment of DNA sequences. Isolation of bacterium cellulolitic, isolating total DNA, gel electroforesis agarosa, amplified of gen 16S rRNA, determination the DNA barcode, homology DNA barcode, and alignment of DNA sequences. The sample of rumen liquid was taked from RPH , then maked isolation of total DNA to get pure DNA from bacterium whos result and amplification DNA of bacterium cellulolitic separated by electroforesis gel agarosa. Gene of bacterium cellulolitic was isolated based Polymerase Chain Reaction methode. Alignment is done by using BlastN program, then use CLustalW program. It can be concluded that the species of bacteria (FKH_USK_6) in rumen has a closeness with Coronabacter sakazakii bacteria strain PNP8 of 97% according to the sizeof the DNA sequence and the same position on an existing gene in GenBank.

TOTAL BAKTERI PSIKOTROPIK IKAN NILA (Oreochromis niloticus) YANG DIBERI PENINGKATAN SUHU PADA SAAT PEMELIHARAAN (Total of Phsycotrophic Bacteria of Nile Tilapia (Oreochromis niloticus) Reared in High Water Temperature) Safni Kamal; Nurliana Nurliana; Faisal Jamin; Sulasmi Sulasmi; Hamny Hamny; Fakhrurrazi Fakhrurrazi
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v10i1.4035

This research aimed to calculate the number of phsycotrophic bacteria of nile tilapia reared in increased water temperature. For this purpose, 12 nile tilapias with body weight of 35-40 g were randomly allotted into 2 treatments groups with 3 replications. Fish in group 1 (P1) was reared in normal temperature (29±1° C) and group II (P2) was reared in high water temperature (35±1 C). All fish were fed commercial diet 3 times a day for 21 consecutive days. T-test analysis revealed a significant decrease in number of psychotropic bacteria (P0.01) in group P2. Total plate count (TPC) resulted in fish gills, intestinal, and muscle were 1.5×10³, 7.7x10³, and 9.7x10³ cfu/g respectively. In conclusion, increasing of water temperature reduce the number of phsycotrophic bacteria of nile tilapia.

IDENTIFIKASI BAKTERI Staphylococcus aureus PADA PREPUTIUM DAN VAGINA KUDA (Equus caballus) (Identification of Staphylococcus aureus in Preputium and Vagina of Horses (Equus caballus)) Yulina Rahmi; Darmawi Darmawi; Mahdi Abrar; Faisal Jamin; Fakhrurrazi Fakhrurrazi; Yudha Fahrimal
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3805

This research aims to identify the Staphylococcus aureus (S. aureus) bacteria from preputium and vagina of horses. Five swabs preputium and 5 swabs vagina samples from 3-9 years olds horse in the village of Bebangka, Gelelungi, Reloop, and Kelapeng, Pegasing Aceh Tengah District were used in this study. Preputium and vagina swabs of horses were dipped into reaction tube containing Nutrient Broth (NB). Staphylococcus aureus was identified on manitol salt agar (MSA). Separated colony colored bright yellow on the media were tested for Gram staining, hemolysis, catalase, and sugar test (glucose and manitol). The result showed that the isolates growth on MSA were colored bright yellow, Gram positive, β hemolisis, and catalase positive. The isolates fermented manitol and glucose. Isolates identified from horses preputium and vagina showed the characteristic of S. aureus. In conclusion S. aureus bacteria were positively identified from all of swabs samples, both in preputium and vagina of horses.Key words: Staphylococcus aureus, horse, preputium, vagina

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