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Journal : Biospecies

Pemanfaatan Acetobacter xylinum terhadap Peningkatan Kualitas Nata de Banana Skin (Utilization of Acetobacter xylinum to improve the quality of Nata de Banana Skin) Harlis HARLIS; Pinta MURNI; Muswita MUSWITA
Biospecies Vol. 8 No. 1 (2015): Januari 2015
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v8i1.2200

Abstract

Banana (Musa sp.) is the most widely consumed fruits since the children age up to the elderly. This is because bananas have quite complete nutrient content such as carbohydrates, proteins, vitamins and minerals, making it beneficial for the human body. People normally only consume and discard any banana skin. Therefore; it needs solutions to deal with the banana peel waste. Banana peels contain high enough nutrients that are carbohydrate 18.5%, phosphorus 28 mg, water 72 g, and some other chemical substances. The high nutrient content on the banana peel is potential to be used as food, one of which is in the form of nata. Nata is a product of microbial fermentation using Acetobacter xylinum. Nata can be made from coconut water waste, liquid waste or waste bark out pineapple and banana peel waste (Nata de Banana Skin). This study aimed to determine the effect of various types of banana peels using A. xylinum on the quality of nata de banana skin. The results showed that different types of banana peel affect the quality of Nata de Banana Skin, both on its flavor and its yield of Nata de Banana Skin. The pisang raja skins give the most optimal results for nata thickness and also perform the best organoleptic values.
Pengaruh Ekstrak Buah Bintaro (Cerbera odollam Gaertn.)Terhadap Petumbuhan Jamur Microsporum canis : Pengaruh Ekstrak Buah Bintaro (Cerbera odollam Gaertn.)Terhadap Petumbuhan Jamur Microsporum canis Farya, Anisa Istiq; Harlis, Harlis; Muswita, Muswita; Budiarti, Retni Sulistiyoning; Mataniari, Raissa; Aswan, Dara Mutiara
Biospecies Vol. 16 No. 2 (2023): Juli 2023
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v16i2.29711

Abstract

This research aims to determine the effect of testing bintaro fruit extract (C. odollam) on the growth of M. canis fungus and to determine the optimal concentration of bintaro fruit extract (C. odollam) on the growth of M. canis fungus. This research used a Complete Randomized Design (RAL) design consisting of 5 treatments, namely P0: ketoconazole control, bintaro fruit extract concentration P1: 25%, P2: 50%, P3: 75%, P4: 100% with 5 repetitions. The parameters observed were the area of the inhibitory zone formed and the optimal concentration of bintaro fruit extract (C. odollam) in inhibiting the growth of M. canis. The data obtained were analyzed using ANOVA and continued with DNMRT test at a 95% confidence level. The results showed that the average diameter of the smallest inhibitory zone in the control was 12.53 mm. The average diameter of the largest inhibitory zone was at 24.10 mm at a concentration of 100% which was not significantly different from the average inhibitory zone at a concentration of 75%, but significantly different from concentrations of 25%, 50% and controls. The conclusion in this research is the effect of giving bintaro fruit extract (C. odollam) on the growth of M. canis fungus and the optimal concentration of bintaro fruit extract (C. odollam) at a concentration of 75%. Keywords: antifungal, Cerbera odollam, fruit extract, Microsporum canis   Abstrak Penelitian ini bertujuan untuk mengetahui pengaruh pengujian ekstrak buah bintaro (C. odollam) terhadap pertumbuhan jamur M. canis dan untuk mengetahui konsentrasi optimal dari ektrak buah bintaro (C. odollam) terhadap pertumbuhan jamur M. canis. Penelitian ini menggunakan rancangan Rancangan Acak Lengkap (RAL) yang terdiri dari 5 perlakuan yaitu P0: kontrol ketokonazole, ekstrak buah bintaro konsentrasi P1: 25%, P2: 50%, P3: 75%, P4: 100% dengan 5 kali pengulangan. Parameter yang diamati adalah luas zona hambat yang terbentuk dan konsentrasi ekstrak buah bintaro (C. odollam) yang optimal dalam menghambat pertumbuhan M. canis. Data yang diperoleh dianalisis dengan menggunakan ANOVA dan dilanjutkan dengan uji DNMRT pada taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa rata-rata diameter zona hambat yang terkecil yaitu pada kontrol yaitu 12,53 mm. Ratarata diameter zona hambat yang terbesar yaitu pada 24,10 mm pada  onsentrasi 100% yang tidak berbeda nyata dengan rata-rata zona hambat pada konsentrasi 75%, namun berbeda nyata dengan konsentrasi 25%, 50% dan kontrol. Oleh sebab itu, dapat disimpulkan bahwa pemberian ekstrak buah bintaro (C. odollam) berpengaruh terhadap pertumbuhan jamur M. canis dan konsentrasi 75% merupakan konsentrasi optimal ekstrak buah bintaro (C. odollam) dalam menghambat pertumbuhan M. canis. Kata kunci: antifungi, Cerbera odollam, ekstrak buah, Microsporum canis  
Uji Aktivitas Anti Bakteri Ekstrak Patikan Kerbau (Euphorbia hirta L.) Terhadap Pertumbuhan Bakteri Penyebab Diare (Eschercia coli) Harlis, Harlis
Biospecies Vol. 3 No. 2 (2010): Februari 2010
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v3i2.248

Abstract

Patikan kerbau (Eupobia hirta L.) contains some anti-diarrheal compounds or containanti-bacterial substances which are alkaloids, flavonoids and tannins. This study aims to determine theeffect of extract of patikan kerbau on the growth of bacteria causing diarrhea (E.coli). This researchtests phytochemical properties and examines inhibition zone (hallow zone). The design used wasCompletely Randomized Design (CRD) with six treatment of patikan kerbau extract (0 ppm, 1500 ppm,1750 ppm, 2000 ppm, 2250 ppm and 2500 ppm) and four replications. Data were analyzed by usingANOVA and if there is a significant difference, the test will be followed by a DNMRT test at 5%significance level. Phytochemical test was based on the formed color intensity. The results showedthat - average inhibition zone diameter on treatment 2500 ppm with a diameter of 10.75 mm was notsignificantly different from 2250 ppm treatment with diameter of 9.75 mm. However, it wassignificantly different with treatments of 2000 ppm, 1750 ppm, 1500 ppm and control. The controlgroup (0 ppm) with a diameter of 6 mm (no inhibition zone formed) was significantly different fromother treatments. Phytochemical test showed that the active compound contained in patikan kerbauare alkaloids, flavonoids and tannins. These compounds have anti-bacterial property and can be usedas an anti-bacteria.
Effect of Type and Arbuskular Mikoriza Mushroom Dosage (CMA) to Growth of Chili [Capsicum annuum L.] at Ultisol Soil Harlis, Harlis; Murni, Pinta; Fitria, Ayu Billy
Biospecies Vol. 1 No. 2 (2008): Agustus 2008
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v1i2.270

Abstract

The research has objective to know the effect of type and arbuskular mikoriza mushroom doses (CMA)to growth of chili (Capsicum annuum L.) at ultisol soil. This research used Completely Randomize Design of Factorialpattern consisting of two factor, that is : first factor of mikoriza type which consist of 3 level, j1 = Glomus sp., j2 =Gigaspora sp. and j3 = Glomus sp. and Gigaspora sp., than second factor of mikoriza doses which consist of 4level, d0 = 0 g, d1 = 5 g, d2 = 10 g. and d3 = 15 g. Data analyzed to use ANOVA and test continued DNMRT at 5% reallevel. The result showed that arbiskular mikoriza doses and type was significantly different to high plant, biomass growon and content of P. Type Gigaspora sp. with dose 15 g can be improve P element absorption and optimal growthvegetative.
Pengaruh Asam Giberelat (Ga3) Terhadap Perkecambahan dan Pertumbuhan Vegetatif Duku (Lansium Dookoo Griff.) Murni, Pinta; Harjono, Danang Puspo; Harlis, Harlis
Biospecies Vol. 1 No. 2 (2008): Agustus 2008
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v1i2.271

Abstract

Penelitian ini bertujuan mengetahui pengaruh Asam Giberelat (GA3) terhadap perkecambahan danpertumbuhan vegetatif duku. Perlakuan dengan pemberian GA3 pada beberapa konsentrasi yaitu 10 ppm, 50 ppm,100 ppm, 150 ppm, 200 ppm, 250 ppm, dan 0 ppm sebagai kontrol. Penelitian menggunakan Rancangan AcakLengkap (RAL) dengan empat kali ulangan. Pengamatan dilakukan terhadap waktu perkecambahan, dayaperkecambahan, tinggi tanaman, dan biomas (berat daun spesifik). Hasil analisis ragam menunjukkan bahwapemberian GA3 pada konsentrasi yang berbeda memberikan pengaruh berbeda nyata terhadap waktuperkecambahan dan daya perkecambahan (kecambah normal),. Berdasarkan hasil penelitian disimpulkan bahwa GA3dapat mempercepat perkecambahan biji duku, konsentrasi optimal untuk parameter perkecambahan yang diamatiadalah 100 dan 150 ppm.
Pengaruh Ekstrak Daun Sirih (Piper betle Linn.) Terhadap Pertumbuhan Bakteri Streptococcus viridans Harlis, Harlis; Wahyuni, Indah
Biospecies Vol. 1 No. 1 (2008): Februari 2008
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v1i1.277

Abstract

Penelitian ini dilakukan untuk melihat pengaruh dari ekstrak daun sirih terhadap pertumbuhan bakteriStreptococcus viridans penyebab penyakit karies gigi. Rancangan percobaan yang digunakan adalahRancangan Acak Lengkap (RAL) dengan 6 perlakuan konsentrasi (0%, 10%, 30%, 50%, 70% dan 90%).Pengamatan dilakukan terhadap diameter daerah hambat menggunakan kertas cakram. Berdasarkan hasilpenelitian diperoleh rata-rata diameter daerah hambat (zona hallow) tertinggi pada pemberian konsentrasi90%, tidak berbeda nyata dengan konsentrasi 50% dan 70%. Selanjutnya pemberian konsentrasi ekstrakdaun sirih 0% dan 10% memberikan hasil terendah terhadap diameter daerah hambat dan berbeda nyatadengan perlakuan lainnya. Konsentrasi ekstrak daun sirih 50%-90% merupakan konsentrasi yang terbaikdalam menghambat pertumbuhan bakteri Streptococcus viridans.
Optimasi Pembentukan Gula Cair Dari Limbah Kulit Singkong (Manihot utilisima Pohl) Oleh Bacillus Licheniformis dalam Usaha Menumbuhkan Jiwa Kewirausahaan. Budiarti, Retni S; Harlis, Harlis; Kapli, Hari
Biospecies Vol. 11 No. 2 (2018): Juli 2018
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (224.827 KB) | DOI: 10.22437/biospecies.v11i2.5745

Abstract

This study aims to determine the optimal concentration of Bacillus licheniformis required in the formation of liquid sugar from cassava waste, the value of sugar and value starch contained in degraded cassava skin waste. The design used in this study was Completely Randomized Design (RAL) with 4 treatment concentration of cassava leaf extract (Manihot utilissima Pohl.), Ie 0 concentration, 1.5 ml concentration, 2.5 ml concentration and 3.5 ml of concentration B. licheneformis. Data were analyzed using analisis (ANOVA) followed by Duncan's range test (DMRT) with a 5% confidence level. The results of the study showed that all treatments had a significant effect on the administration of B. licheniformis concentrations. Treatment using 1.5 ml B. licheniformis concentration on cassava skin substrate weighing 1 gram after Duncan's test range (DMRT) with 5% confidence level resulted Reduction sugar value of 6.07%.
Produksi Pupuk Cair dari Isolat Bakteri Limbah Sayur Pasar Angso Duo Jambi dalam Meningkatkan Perekonomian dan Kesehatan Lingkungan Masyarakat Jambi Harlis, Harlis; Budiarti, Retni S; Kapli, Hari; Sanjaya, M Erick
Biospecies Vol. 12 No. 1 (2019): Januari 2019
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.195 KB) | DOI: 10.22437/biospecies.v12i1.6577

Abstract

Angso Duo Market is the largest traditional market in the city of Jambi, with an area of ​​± 5 Ha. The amount of waste produced by Angso Duo market every day is around 105 tons per day with a ratio of 70% biodegradable waste, 25% recycled (organic and paper) and 5% other waste. Of the 75% of waste that is around 60% is waste in the form of vegetables, most of which are mustard and cabbage. Vegetable waste has great potential to be used as organic fertilizer because it has good and complex nutrients such as protein, fat carbohydrates, calcium, phosphorus, iron, vitamins A, B and C, folic acid, fiber, water and sodium. The advantage of liquid organic fertilizer is that it contains enough nitrogen as a constituent of plant protein and chlorophyll. The results showed 6 types of bacteria namely Planococus, Neisseria, Halobacter, Azomonas, Azotobacter and Bruccella which were formulated into liquid fertilizers with a basic substrate of vegetable cabbage and mustard waste.Then tested the ability of bacteria to dissolve nitrogen, phosphorus and potassium as potential bacteria as liquid fertilizer
ZOOPLANKTON KEANEKARAGAMAN ZOOPLANKTON SEBAGAI INDIKATOR KUALITAS DANAU SIPIN KOTA JAMBI SEBAGAI PENGAYAAN PRAKTIKUM TAKSONOMI MONERA DAN PROTISTA Pangestu, Retno; SUKMONO, TEDJO; HARLIS, HARLIS
Biospecies Vol. 13 No. 2 (2020): Juli 2020
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v13i2.8374

Abstract

Abstract. This study aims to determine the diversity of zooplankton in Lake Sipin. This study uses a descriptive exploratory method by determining the location by purposive sampling. The results showed that found 6 zooplankton classes, namely: Tubulinea, Monogononta, Maxillopoda, Euglenophyceae, Imbricatea, and Eurotatoria consisting of 15 types, namely: Arcella sp., Difflugia globulosa, Difflugia limnetica, Difflugia pyriformis, Asplanchna sp., Brachionus sp., Cyclopoid sp., Macrocyclops varians, Trachelomonas rachiborskii, Euglypa cristata, Polyartha trigla, Trichocerca sp., Trichocerca birostris, Trichocerca bicristata. Abundance ranges from 0.0031 Ind / L - 0.0051 Ind / L; Evenness index ranges from 0.876 - 0.915; diversity index 1.41 - 2.164; and a dominance index of 0.128 - 0.292. Diversity is classified as moderate because the diversity index obtained is greater than 1 and smaller than 3 and no species dominates. It can be concluded that the highest diversity is found in the Tubulinea class. Lake water quality can be tolerated by zooplankton because it tends to have good value. Researchers are advised to conduct research at night to see the relationship between the distribution of zooplankton with the intensity of sunlight, as well as conducting research by determining the location of more diffuse points.