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Optimasi Sterilisasi Eksplan Biji Manggis (Garcinia mangostana L.) Menggunakan Natrium Hipoklorit Secara In vitro Harahap, Fauziyah; Asri, Elita; Hasairin, Ashar; Edi, Syahmi; Suriani, Cicik; Daulae, Abdul Hakim; Panggabean, Nurul Huda
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15281

Abstract

This study evaluates the effects of NaOCl concentration and immersion time on contamination levels and viability of in vitro mangosteen seed explants, aiming to identify the optimal sterilization protocol. Conducted at YAHDI Tissue Culture Laboratory, Medan, Indonesia, a Randomized Complete Block Design tested three treatment combinations: (1) 15% and 10% NaOCl for 5 minutes, (2) 20% and 15% NaOCl for 15 minutes, and (3) 30% and 20% NaOCl for 25 minutes. After immersion, explants were rinsed three times with sterile distilled water. Observations spanned 12 MST, with data analyzed using ANOVA and Games-Howell post hoc tests; contamination sources were qualitatively described. Results showed the first combination had the lowest effectiveness, the second was better but suboptimal, while the third significantly delayed contamination and yielded the highest explant survival rate of 86.1%. The third treatment also prolonged the contamination-free period compared to others. Fungal and bacterial contaminants were predominant, highlighting the need for effective disinfectants against both. These findings indicate that the third combination is a promising sterilization protocol for mangosteen seed explants, especially for hard endocarp tissues prone to contamination.
Pengembangan Instrumen Penilaian Afektif Berbasis Self Assesment pada Materi Biodiversity Kelas X di SMA Negeri 9 Medan Nainggolan, Farizah Handayani; Djulia, Ely; Hasairin, Ashar
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15497

Abstract

This study aims to develop an affective assessment instrument based on self-assessment of biodiversitymaterial class X at SMA Negeri 9 Medan. This research uses research methods used to produce certain products and test the validity and practicality of products with the Thiagarajan development model (4D) which consists of 4 stages of development, namely define, design, develop and disseminate. The subject of this study is class X at SMA Negeri 9 Medan which consists of 108 students. This research focuses on the affective realm of science, namely in the form of scientific attitudes, which include indicators of curiosity, objectivity, cooperation, responsibility, open thinking, and discipline. This development stage consists of instrument validation, reliability analysis and instrument testing. The results of the study showed that (1) after being validly tested with an average value of 3.52 with the category of very feasible using SPSS data. (2) Viewed as a whole from the Alpha Cronbach coefficient, it can be concluded that the assessment device is declared reliable on the high reliability criteria because the test scores in classes X1, X2, and X3 are 0.821, 0.821, and 0.836. (3) The results of the trial that have been carried out are concluded that the effectiveness criteria have been achieved with the average score of the three respondents being 82%.
Analisis Kemampuan Berpikir Tingkat Tinggi Siswa pada Materi Keanekaragaman Hayati di Kelas X Stivani, Elly; Djulia, Ely; Hasairin, Ashar
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.16211

Abstract

This study aims to determine the students' higher order thinking skills on biodiversity material in class X. This research uses a quantitative descriptive approach. The sample of this study was class X students consisting of 104 people determined by cluster random sampling technique. The instrument used was a test question in the form of an essay of 12 items on biodiversity material. The results showed that students' higher order thinking skills on biodiversity material in class X were at very low criteria with the acquisition of an average student score of 44.5%. In each indicator, it was found that the C4 indicator of class X students was very low with a percentage of 44.3%, the C5 indicator was very low with a percentage of 40.0%, and the C6 indicator was very low with a percentage of 46.0%. Thus, it can be concluded that to be able to improve the higher order thinking skills of grade X students, students begin to familiarize themselves with reading various literatures, solve HOTS-based problems, and students must use various learning strategies.
Etnomedisin Param (Kuning) sebagai Obat Tradisional di Desa Bunuraya Kabupaten Karo Nensi Grafika Br Sinuraya; Ashar Hasairin
Jurnal Pendidikan Sains dan Teknologi Terapan | E-ISSN : 3031-7983 Vol. 1 No. 4 (2024): Oktober - Desember
Publisher : CV.ITTC INDONESIA

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Abstract

This study aims to document the use of plants used in making param (kuning) as a traditional medicine of the Karo Tribe in Bunuraya Village so that the knowledge of the community is not lost with the progress of the times. The location of the research was carried out in Bunuraya Village, Tigapanah District, Karo Regency. The methods used in this study are qualitative and quantitative descriptive methods, using semi-structured interview methods and participatory observation. Research result show that the number of plants used as raw material for param (kuning) is 22 species covering 13 families. The most widely used percentage of plant families is the Zingiberaceae family with a percentage of 22.72%. The most widely used organ is the rhizome organ with a percentage of 22.72%. The most widely used plant habitus is herbs with a percentage of 63.63%. The origin of the most plants obtained is cultivation with a percentage of 54.54%. The Karo Tribe people believe that the use of plants in making param (kuning) as a traditional medicine can treat various diseases, improve health and maintain health. The important cultural value in this study is divided into 4 categories, namely very high, high, medium and low. Very high values consist of 5 species with a value of 100, high values consist of 11 species with a value (66-99), medium values consist of 5 species with a value (24-40) and low values consist of 1 species with a value of 12. The use value of param (kuning) as the highest traditional medicine consists of 16 benefits with a value of 0.72.
Etnobotani Tumbuhan Pada Upacara Adat Patuaekkon Masyarakat Etnis Mandailing Di  Aliran Sungai Batang Lubu Kabupaten Padang Lawas Fepi Hasibuan; Ashar Hasairin; Adi Hartono
Jurnal Pendidikan Sains dan Teknologi Terapan | E-ISSN : 3031-7983 Vol. 1 No. 4 (2024): Oktober - Desember
Publisher : CV.ITTC INDONESIA

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Abstract

Penelitian ini bertujuan untuk Melihat pandangan masyarakat Suku Mandailing terhadap pemanfaatan tumbuhan, Spesies yang digunakan, tumbuhan yang digunakan, bentuk pemanfaatan dan indeks nilai penting budaya (ICS) tumbuhan dalam upacara adat pernikahan Mandailing. Lokasi penelitian dilakukan di 3 Desa yaitu Desa Botung, Desa Aek Sorik dan Desa Huta Godang, Kecamatan Batang Lubu Sutam, Kab. Padang Lawas. Metode penelitian bersifat kualitatif dan kuantitatif. Pengumpulan data dilakukan melalui wawancara, observasi, dokumentasi dan identifkasi tumbuhan. Hasil penelitian menunjukkan bahwa tumbuhan yang digunakan dalam upacara adat Pernikahan Suku Mandailing berjumlah 10 spesies yang mencakup 7 famili tumbuhan. Famili yang banyak digunakan berasal dari famili Rutaceae sebanyak 3 spesies. Organ daun merupakan organ tumbuhan yang banyak digunakan dengan persentase 36,36%. Habitus tumbuhan yang banyak digunakan adalah Perdu sebanyak 6 habitus, Nilai penting budaya pada penelitian ini terbagi atas dua kategori yaitu kategori tinggi dan sedang. Nilai tinggi terdiri dari 7 spesies dengan nilai dari 50 sampai 91, nilai sedang terdiri dari 3 spesies dengan nilai dari 43 sampai 49.
Analisis Berbagai Jenis Tanaman yang dapat Dimanfaatkan Sebagai Obat, Kosmetik, Rempah, dan Bumbu: Analisis Berbagai Jenis Tanaman Aritonang, Putri; Akbar, Habil; Sinaga, Maya Febrina; Sinaga, Nadia Rouli; Sastra, Siti Fathiya Dwinda; Sinaga, Yefta Darniati; Nasution, Aswarina; Hasairin, Ashar
Jurnal Sains Indonesia Vol. 6 No. 3 (2025): Vol 6 No 3 (2025): Volume 6, Nomor 3, 2025 (November)
Publisher : PUSAT SAINS INDONESIA

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Abstract

This study aims to examine the use of medicinal plants by the community based on local knowledge that has been passed down from generation to generation. An ethnobotanical approach is used to explore information about the types of medicinal plants, the parts of the plants that are used, how to process them, and the types of diseases that can be treated. Data were collected through literature studies of books, scientific journals, research reports, and other related documents. The results of the study indicate that the community is still very dependent on medicinal plants in their daily medical practices. Various types of plants were found to be used to treat mild to chronic diseases, with processing methods such as boiling, pounding, or brewing. These findings emphasize the importance of preserving local knowledge and conserving medicinal plants as part of a cultural heritage that has great potential in the development of alternative medicine.
Korelasi Tugas Rekayasa Ide, Mini Riset, Dan Proyek Terhadap Minat Meneliti Mahasiswa BIologi Pada Mata Kuliah Taksonomi Spermatophyta Di Universitas Negeri Medan Hasanah, Dwi; Hasairin, Ashar
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 2 (2025): September 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i2.12813

Abstract

Tugas Rekayasa Ide mendorong mahasiswa untuk berpikir lebih kritis dan mengasah kemampuan mereka dalam mengemukakan ide-ide kreatif. Mini riset merupakan salah satu dari enam tugas KKNI, bertujuan untuk melatih mahasiswa dalam melakukan penelitian dan merupakan lanjutan dari tugas Rekayasa Ide. Tugas Proyek merupakan hasil akhir dari tugas Mini Riset. Oleh karena itu, ketiga tugas ini saling terkait dan memiliki hubungan yang erat dalam mendorong minat penelitian di kalangan mahasiswa. Dengan demikian, peneliti memilih ketiga tugas ini sebagai fokus penelitian untuk mengamati minat penelitian mahasiswa Jurusan Biologi di Universitas Negeri Medan. Penelitian terdahulu yang dilakukan oleh Maulidiya (2020) dengan judul “Hubungan Pelaksanaan Tugas Rekayasa Ide, Mini Riset dan projek Pada Mata Kuliah MikroBiologi Terhadap Minat Meneliti Mahasiswa Biologi Universitas Negeri Medan Angkatan 2017”
Inventarisasi Tanaman Obat Keluarga di Pekarangan Rumah Masyarakat Suku Jawa di Desa Perkebunan Ramunia Kecamatan Pantai Labu Provinsi Sumatera Utara Fathimatuzzahro'a, Fathimatuzzahro'a; Hasairin, Ashar
BIO-CONS : Jurnal Biologi dan Konservasi Vol. 7 No. 2 (2025): BIO-CONS: Jurnal Biologi dan Konservasi
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biocons.v7i2.2497

Abstract

Penelitian ini bertujuan untuk menginventarisasi tanaman obat keluarga yang ditanam di pekarangan rumah masyarakat Suku Jawa di Desa Perkebunan Ramunia, Kecamatan Pantai Labu, Kabupaten Deli Serdang, Provinsi Sumatera Utara. Penelitian dilakukan dengan metode deskriptif kualitatif dan kuantitatif melalui observasi langsung dan wawancara semi-terstruktur kepada 16 informan yang dipilih menggunakan teknik purposive sampling. Data dikumpulkan berupa spesies tanaman, organ yang dimanfaatkan, habitus, serta cara pengolahan dan penggunaan tanaman. Hasil penelitian menunjukkan bahwa terdapat 52 spesies tanaman obat yang termasuk dalam 34 famili, dengan bagian tanaman yang paling banyak dimanfaatkan adalah daun (46%), diikuti oleh rimpang dan buah. Habitus tanaman yang paling dominan adalah terna (54%), kemudian perdu dan pohon. Cara pengolahan tanaman yang paling sering digunakan adalah direbus (58%), sedangkan cara penggunaan yang umum adalah dengan diminum (58%). Penelitian ini menunjukkan bahwa masyarakat Suku Jawa masih memanfaatkan tanaman obat sebagai alternatif pengobatan tradisional yang diwariskan secara turun-temurun, meskipun pengetahuannya belum terdokumentasi secara sistematis. Oleh karena itu, penting dilakukan dokumentasi ilmiah untuk pelestarian pengetahuan tentang tanaman obat keluarga.  
Peran Vital Kepemimpinan Himpunan Masyarakat Pakpak (HIMPAK) Dalam Mempertahankan Jati Diri Silima Suak Ujung, Siti Nuraisah A; Amanda, Putri; Harahap, Fauziyah; Hasairin, Ashar
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 2 (2025): September 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i2.13232

Abstract

Penelitian ini mengulas peran krusial kepemimpinan Himpunan Masyarakat Pakpak (HIMPAK) dalam mempertahankan dan memperkuat jati diri Silima Suak sebagai fondasi identitas Suku Pakpak. Metode penelitian yang digunakan adalah pendekatan kualitatif deskriptif. Pengumpulan data dilakukan melalui wawancara terstruktur dengan Ketua Harian sebagai sumber primer, serta studi dokumentasi sebagai sumber sekunder. Data dianalisis melalui tahapan reduksi, penyajian, dan penarikan kesimpulan untuk memperoleh gambaran mendalam tentang peran dan filosofi kepemimpinan HIMPAK dalam konteks budaya Suku Pakpak. HIMPAK memposisikan diri sebagai “rumah bersama” yang secara efektif menjembatani tradisi dan modernitas. Temuan utama menunjukkan bahwa kepemimpinan HIMPAK menerapkan prinsip inklusif dengan memastikan adanya keterwakilan yang adil dari seluruh Silima Suak dalam struktur kepengurusan. Pengambilan keputusan organisasi dijiwai oleh filosofi luhur, yang menuntun pada musyawarah mufakat. Fokus program HIMPAK tidak hanya pada pelestarian seni, budaya, dan adat, tetapi juga pada kaderisasi pemuda untuk kelanjutan tradisi, serta perhatian pada peningkatan kesejahteraan ekonomi masyarakat (melalui IKM dan Koperasi)